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The map label for this gene is eda [H]

Identifier: 54293414

GI number: 54293414

Start: 507040

End: 507702

Strand: Direct

Name: eda [H]

Synonym: lpl0463

Alternate gene names: 54293414

Gene position: 507040-507702 (Clockwise)

Preceding gene: 54293413

Following gene: 54293415

Centisome position: 15.16

GC content: 42.84

Gene sequence:

>663_bases
ATGTCCATTTCTGAATGGAAAACACAACCGGGCGCTGTATTTGCTGCATCTGCGGTTATTCCAGTTATTGTAATCAAAGA
GTTAGAGGATGCGTTACCTTTGGCCGAGGCTTTATTTGCCGGAGGAATTCATGTTCTTGAAGTGACATTAAGAACACCAG
TGGCAATAAAAGCACTGGAATTATTGAACAATACGTTTCCAGATGTATTAATCGGTGCCGGAACCGTGATTACCCCTAGT
CAGTTTCATGATGTGGTAGCAGCAGGCGCTCGATTTGCCATTAGCCCTGGCCAAACAAGGGAATTATTAATAGCAGGACA
GAAAAGTGAAATTCCTCTCATTCCAGGTGTAGCGAGTGTTTCTGAATTAATGGAAGGTTTGAGGATGGGATATAACCATT
TTAAATTTTTTCCTGCGGCAGCGGCTGGAGGCATCCCTATGTTGAAAGCGATATCGGGCGTATTCCCCCAAGTTAAATTT
TGTCCAACAGGCGGAATTAATTCCAAAAATTATGAGGAGTATCTTTGCTTGCCAAATGTAGCTTGCGTTGGAGGGTCCTG
GATTGTACCGGAAGAGGCTATCAAGAATCATAACTGGTCTTTAATTACAGAATTATGTATGGCGGTGAGTTCTCAGAAGC
GAGAACTTTGCTCGCAGAATTAA

Upstream 100 bases:

>100_bases
GATTTTAATGCCTTAATTCCCACTTATGTCATTGCAGCCTCTCAACCAGGTATTTTAGGCGCGAGCGTCAGTTTAAAACA
AATATTGACAGGAGCTTCTC

Downstream 100 bases:

>100_bases
TCTATTCGGGTTGAAAGAGCGTTTTTATATGATGTAAGGGATAGTGCCAGCTAAAACAGTTCATTGTGTAGAGTAGAGAC
AGAGGAAAAGAATTTAAAAT

Product: hypothetical protein

Products: NA

Alternate protein names: 4-hydroxy-2-oxoglutarate aldolase; 2-keto-4-hydroxyglutarate aldolase; KHG-aldolase; 2-dehydro-3-deoxy-phosphogluconate aldolase; 2-keto-3-deoxy-6-phosphogluconate aldolase; KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase [H]

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MSISEWKTQPGAVFAASAVIPVIVIKELEDALPLAEALFAGGIHVLEVTLRTPVAIKALELLNNTFPDVLIGAGTVITPS
QFHDVVAAGARFAISPGQTRELLIAGQKSEIPLIPGVASVSELMEGLRMGYNHFKFFPAAAAGGIPMLKAISGVFPQVKF
CPTGGINSKNYEEYLCLPNVACVGGSWIVPEEAIKNHNWSLITELCMAVSSQKRELCSQN

Sequences:

>Translated_220_residues
MSISEWKTQPGAVFAASAVIPVIVIKELEDALPLAEALFAGGIHVLEVTLRTPVAIKALELLNNTFPDVLIGAGTVITPS
QFHDVVAAGARFAISPGQTRELLIAGQKSEIPLIPGVASVSELMEGLRMGYNHFKFFPAAAAGGIPMLKAISGVFPQVKF
CPTGGINSKNYEEYLCLPNVACVGGSWIVPEEAIKNHNWSLITELCMAVSSQKRELCSQN
>Mature_219_residues
SISEWKTQPGAVFAASAVIPVIVIKELEDALPLAEALFAGGIHVLEVTLRTPVAIKALELLNNTFPDVLIGAGTVITPSQ
FHDVVAAGARFAISPGQTRELLIAGQKSEIPLIPGVASVSELMEGLRMGYNHFKFFPAAAAGGIPMLKAISGVFPQVKFC
PTGGINSKNYEEYLCLPNVACVGGSWIVPEEAIKNHNWSLITELCMAVSSQKRELCSQN

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788156, Length=203, Percent_Identity=51.2315270935961, Blast_Score=204, Evalue=4e-54,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000887
- InterPro:   IPR013785 [H]

Pfam domain/function: PF01081 Aldolase [H]

EC number: =4.1.3.16; =4.1.2.14 [H]

Molecular weight: Translated: 23430; Mature: 23298

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS00159 ALDOLASE_KDPG_KHG_1 ; PS00160 ALDOLASE_KDPG_KHG_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISEWKTQPGAVFAASAVIPVIVIKELEDALPLAEALFAGGIHVLEVTLRTPVAIKALE
CCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHH
LLNNTFPDVLIGAGTVITPSQFHDVVAAGARFAISPGQTRELLIAGQKSEIPLIPGVASV
HHHCCCCHHHHCCCCEECHHHHHHHHHHCCEEEECCCCCCEEEEECCCCCCCCCCCHHHH
SELMEGLRMGYNHFKFFPAAAAGGIPMLKAISGVFPQVKFCPTGGINSKNYEEYLCLPNV
HHHHHHHHHCHHHHEEECCHHCCCCHHHHHHHHCCCCEEECCCCCCCCCCCCCEEECCCE
ACVGGSWIVPEEAIKNHNWSLITELCMAVSSQKRELCSQN
EEECCCEECCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SISEWKTQPGAVFAASAVIPVIVIKELEDALPLAEALFAGGIHVLEVTLRTPVAIKALE
CCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHH
LLNNTFPDVLIGAGTVITPSQFHDVVAAGARFAISPGQTRELLIAGQKSEIPLIPGVASV
HHHCCCCHHHHCCCCEECHHHHHHHHHHCCEEEECCCCCCEEEEECCCCCCCCCCCHHHH
SELMEGLRMGYNHFKFFPAAAAGGIPMLKAISGVFPQVKFCPTGGINSKNYEEYLCLPNV
HHHHHHHHHCHHHHEEECCHHCCCCHHHHHHHHCCCCEEECCCCCCCCCCCCCEEECCCE
ACVGGSWIVPEEAIKNHNWSLITELCMAVSSQKRELCSQN
EEECCCEECCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8145647 [H]