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The map label for this gene is ywbI [H]

Identifier: 54293221

GI number: 54293221

Start: 300655

End: 301539

Strand: Direct

Name: ywbI [H]

Synonym: lpl0269

Alternate gene names: 54293221

Gene position: 300655-301539 (Clockwise)

Preceding gene: 54293216

Following gene: 54293222

Centisome position: 8.99

GC content: 44.41

Gene sequence:

>885_bases
ATGGAATTTAAACTGCTTCAGACTTTCATAGAGGTTGTACGGCAGGGTAGTTTTTCCAAAGCAGCTGAAAATCTATTTGC
GACCCAGTCTACGGTGAGTAAAGCCATAAAACAGCTTGAAGAGGAGTTAGGTATCACTCTGATTGAGCGGTTTAAAAAGC
GTAATGTACCAACAACAGCAGGTGAAATTGTTTATCAGCGAGGAACCAAACTACTGGCTGATCGAGATGATCTTCTTAAA
GAATTGGATGAAATCCGAGGTTTGAAACAAGGTCGACTGCGTTTGGGTATTTCTCCGGTGGGCAGCAGTACTTTATTTGC
CCCTCTTTTTGTTCTGTACAGACAACGTTATCCAGGCATTGAAGTAGAGCTTGTTGAGCATGGCAGCGATAAACTTGCAG
AGTGTTTGCGTGCAGGAGAGATAGATTTTGCTGGAACCTTATTACCCATTTCCGAAGAATTTGATTGCCAATTAGTACGC
TCTGAACCGTTGGTCGCGCTCCTGGCATCTGGACATCCATTAGCAACTAAACATTGCATTTCTCTACCAGAACTTAAAGA
AATACCTTTCATCCTTTTTGGCAGTGGTTTCGCACTACATCGAATTATATTGGACGCGTGCCGTCACGCTGGTTTCGAAC
CTAAGGTAGTGGCTCAAAGCAGTCAGATTGATTTTATGCTGGAGCTTGTTGCAGCAGGCCTTGGGGTTACTTTTTTGCCA
AGGATGATTGCCACTCAAAGGCATACGACACAAATTCGCTCTCTTTTACTTAATGGAAGTGAGTTCCAATGGAATATGGC
GATGACCTGGCGCAGAAACTCTTATCTCTCCGAGGCTGCGCAAGCCTGGTTGGCTCTGGTGCGCGAAGTGCATGTTGATA
ATTAG

Upstream 100 bases:

>100_bases
TGCTCATGATGGATATTATTTTTTCATTTAAGTTTACACTTTTAATTAAGATGAATAAAATGAATTTATAGACTAATAAC
CTTTCCAATATGGAATAATT

Downstream 100 bases:

>100_bases
TTTTAGATTGGGGAATGCAAGAGATCTATTTTCCTATTATCCAAATAAGGCAGGTGTATTGAAAATCATTGCTGGTGTAG
CCATACTTCATATCTAAACT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MEFKLLQTFIEVVRQGSFSKAAENLFATQSTVSKAIKQLEEELGITLIERFKKRNVPTTAGEIVYQRGTKLLADRDDLLK
ELDEIRGLKQGRLRLGISPVGSSTLFAPLFVLYRQRYPGIEVELVEHGSDKLAECLRAGEIDFAGTLLPISEEFDCQLVR
SEPLVALLASGHPLATKHCISLPELKEIPFILFGSGFALHRIILDACRHAGFEPKVVAQSSQIDFMLELVAAGLGVTFLP
RMIATQRHTTQIRSLLLNGSEFQWNMAMTWRRNSYLSEAAQAWLALVREVHVDN

Sequences:

>Translated_294_residues
MEFKLLQTFIEVVRQGSFSKAAENLFATQSTVSKAIKQLEEELGITLIERFKKRNVPTTAGEIVYQRGTKLLADRDDLLK
ELDEIRGLKQGRLRLGISPVGSSTLFAPLFVLYRQRYPGIEVELVEHGSDKLAECLRAGEIDFAGTLLPISEEFDCQLVR
SEPLVALLASGHPLATKHCISLPELKEIPFILFGSGFALHRIILDACRHAGFEPKVVAQSSQIDFMLELVAAGLGVTFLP
RMIATQRHTTQIRSLLLNGSEFQWNMAMTWRRNSYLSEAAQAWLALVREVHVDN
>Mature_294_residues
MEFKLLQTFIEVVRQGSFSKAAENLFATQSTVSKAIKQLEEELGITLIERFKKRNVPTTAGEIVYQRGTKLLADRDDLLK
ELDEIRGLKQGRLRLGISPVGSSTLFAPLFVLYRQRYPGIEVELVEHGSDKLAECLRAGEIDFAGTLLPISEEFDCQLVR
SEPLVALLASGHPLATKHCISLPELKEIPFILFGSGFALHRIILDACRHAGFEPKVVAQSSQIDFMLELVAAGLGVTFLP
RMIATQRHTTQIRSLLLNGSEFQWNMAMTWRRNSYLSEAAQAWLALVREVHVDN

Specific function: Positive Regulatory Protein For The Cyn Operon. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=282, Percent_Identity=29.7872340425532, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1788887, Length=293, Percent_Identity=27.3037542662116, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787879, Length=249, Percent_Identity=28.1124497991968, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1790399, Length=279, Percent_Identity=24.7311827956989, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1788748, Length=300, Percent_Identity=26, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI145693105, Length=295, Percent_Identity=24.406779661017, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787530, Length=246, Percent_Identity=24.7967479674797, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI2367136, Length=249, Percent_Identity=25.7028112449799, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1790208, Length=246, Percent_Identity=26.0162601626016, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787601, Length=171, Percent_Identity=28.0701754385965, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1789506, Length=294, Percent_Identity=22.7891156462585, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1789204, Length=247, Percent_Identity=23.4817813765182, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788296, Length=249, Percent_Identity=26.9076305220884, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790262, Length=254, Percent_Identity=27.5590551181102, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786401, Length=120, Percent_Identity=35, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788297, Length=247, Percent_Identity=25.5060728744939, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI145693193, Length=141, Percent_Identity=34.0425531914894, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1788481, Length=204, Percent_Identity=26.9607843137255, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789639, Length=123, Percent_Identity=32.520325203252, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787589, Length=174, Percent_Identity=26.4367816091954, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787128, Length=173, Percent_Identity=30.0578034682081, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32935; Mature: 32935

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFKLLQTFIEVVRQGSFSKAAENLFATQSTVSKAIKQLEEELGITLIERFKKRNVPTTA
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCH
GEIVYQRGTKLLADRDDLLKELDEIRGLKQGRLRLGISPVGSSTLFAPLFVLYRQRYPGI
HHHHHHHCCHHHCCHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCC
EVELVEHGSDKLAECLRAGEIDFAGTLLPISEEFDCQLVRSEPLVALLASGHPLATKHCI
EEEEECCCCHHHHHHHHCCCCCCCCEEECCCCCCCCEEECCCCEEEEEECCCCCHHHHHC
SLPELKEIPFILFGSGFALHRIILDACRHAGFEPKVVAQSSQIDFMLELVAAGLGVTFLP
CCCCHHHCCEEEECCCHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHCCHHHHH
RMIATQRHTTQIRSLLLNGSEFQWNMAMTWRRNSYLSEAAQAWLALVREVHVDN
HHHHHHHHHHHHHHHHHCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEFKLLQTFIEVVRQGSFSKAAENLFATQSTVSKAIKQLEEELGITLIERFKKRNVPTTA
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCH
GEIVYQRGTKLLADRDDLLKELDEIRGLKQGRLRLGISPVGSSTLFAPLFVLYRQRYPGI
HHHHHHHCCHHHCCHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCC
EVELVEHGSDKLAECLRAGEIDFAGTLLPISEEFDCQLVRSEPLVALLASGHPLATKHCI
EEEEECCCCHHHHHHHHCCCCCCCCEEECCCCCCCCEEECCCCEEEEEECCCCCHHHHHC
SLPELKEIPFILFGSGFALHRIILDACRHAGFEPKVVAQSSQIDFMLELVAAGLGVTFLP
CCCCHHHCCEEEECCCHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHCCHHHHH
RMIATQRHTTQIRSLLLNGSEFQWNMAMTWRRNSYLSEAAQAWLALVREVHVDN
HHHHHHHHHHHHHHHHHCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]