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The map label for this gene is yhjG [H]

Identifier: 54293192

GI number: 54293192

Start: 263695

End: 265137

Strand: Direct

Name: yhjG [H]

Synonym: lpl0238

Alternate gene names: 54293192

Gene position: 263695-265137 (Clockwise)

Preceding gene: 54293191

Following gene: 54293193

Centisome position: 7.88

GC content: 43.38

Gene sequence:

>1443_bases
ATGAGCGCACAAGTTGCAGACGTTGTAATTGTCGGTGCGGGTCCAGTAGGGCTCATGTGTGCTTATTTGGGACAACTTTG
TGGTATCCATACCGTGATTGTCGATAAGTCTGATGGTCCTTTAGAGGTCGGTAGAGCGGATGCCCTTAACGCGCGCACTT
TACAACTTTTCGAATTAGTTGATTTATTTGATGAACTTTATCCTTTAGGAAAGACCTGCAATACCAGTTCTGTTTGGGCA
GATGGACAATTCATTTCCCGACAATCGTCATGGTGGGAGGAATTGGAGGGTTGTTTGCATAAACATTTCCTTATGCTTGG
TCAATCCTATGTAGAAAAATTGTTAGATGACAAGCTGAAGGAAACTGTGGCAGCAGTAAAACGTTCAACAGCGATTGTCA
ACATTGAGATTAATGAAGCAGGATGTCTTACGACTCTTGCCAATGGAGAGCGTATCCAGTCACGCTACGTCATCGGAGCC
GATGGGGCTCGTTCCTTTGTTCGCAACCACTTTGCAATACCTTTTGAGATCATACGGCCACAAATTATATGGGCGGTCAT
CGATGGTATGATTGAAACCGACTTTCCAAAAGTCCCTGAAATCATCGTGTTTCAGGCAGAAACTTCGGATGTGGCCTGGA
TTCCAAGGGAAGGGGAAATCGACAGGTTTTACGTAAGAATGGATACTAAAGACTTTACCTTGCAGGAAGCAATGGACAAA
ATTAATCATGCTGTGCGGCCCCATTCCTTAAATTTTAAGAACATTGTCTGGTTTTCACAATTTTCAGTTAAAGAATCTGT
CGCTGAGCGTTTCTTCATTCAGAATCGCATTTTTCTTGCCGGTGACTCCTGCCATATTCATTCGGTAAACGGTGGGCAAG
GTCTTAACACTGGTCTTGCAGATGCGTTTAATCTCATGTGGAAATTGCACATGGTCATGCATTTTGGTGCCCCCAAAGAA
CTTTTGCACAGTTATGAAGCTGAGCGTAAACCAGTAGCTCACGGTGTGATAGAGACTTCCGGTGAGCTTGTGCGCTCGAC
CAAGTATTCGCTTAATGGAACCCATGCCCAAGACTATGTCAAGATTGTGCAAAAGCGCGCGGGTTATATCACTGGGATGG
GGATTCGTTATGGAGAAACAGGTTTGTGTGGTTCTCGACTTTTTGATTTTGAAATTTTTAATGGCATTGCCAAAACACGG
CTTTACTCTCTTTTGGATTATATGAAATTCACTTTATTTATCTTTGGTGACCGTGAAGTCGAGTTACAGCCTCCGGAGTT
TGTCAAAATCATTCCAATATATCCAAACCAATGCCAGGAAAATTTTTGGACCAATAACACACACTATGCAAACCAGGCCA
TTTTAGTAAGGCCTGACTCCTACATCCAATACGCTGTGCCGCTGGATAAAATAGAATCCTTACTTGAGTTGGCGCAGTTA
TGA

Upstream 100 bases:

>100_bases
ATGGCAGAGAAGGGTTTGTCTCCAGGTCGCCGCGTCATATCCAACGCGTTCATGTTTTGAGTAATCCGCCTTTTGATAAC
CCGGGTCTGGAGTCTTATCA

Downstream 100 bases:

>100_bases
AGAAAAGCCTGATTTTTTTAGCAATAGGCATGTTTACAGTAGGTTGCAATACATTTCTGATTGCTGGTCTGCTTCCTCAA
ATAGGCGAAACGCTTGGGCA

Product: hypothetical protein

Products: H2O; 3-(2,3-dihydroxyphenyl)propionate; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MSAQVADVVIVGAGPVGLMCAYLGQLCGIHTVIVDKSDGPLEVGRADALNARTLQLFELVDLFDELYPLGKTCNTSSVWA
DGQFISRQSSWWEELEGCLHKHFLMLGQSYVEKLLDDKLKETVAAVKRSTAIVNIEINEAGCLTTLANGERIQSRYVIGA
DGARSFVRNHFAIPFEIIRPQIIWAVIDGMIETDFPKVPEIIVFQAETSDVAWIPREGEIDRFYVRMDTKDFTLQEAMDK
INHAVRPHSLNFKNIVWFSQFSVKESVAERFFIQNRIFLAGDSCHIHSVNGGQGLNTGLADAFNLMWKLHMVMHFGAPKE
LLHSYEAERKPVAHGVIETSGELVRSTKYSLNGTHAQDYVKIVQKRAGYITGMGIRYGETGLCGSRLFDFEIFNGIAKTR
LYSLLDYMKFTLFIFGDREVELQPPEFVKIIPIYPNQCQENFWTNNTHYANQAILVRPDSYIQYAVPLDKIESLLELAQL

Sequences:

>Translated_480_residues
MSAQVADVVIVGAGPVGLMCAYLGQLCGIHTVIVDKSDGPLEVGRADALNARTLQLFELVDLFDELYPLGKTCNTSSVWA
DGQFISRQSSWWEELEGCLHKHFLMLGQSYVEKLLDDKLKETVAAVKRSTAIVNIEINEAGCLTTLANGERIQSRYVIGA
DGARSFVRNHFAIPFEIIRPQIIWAVIDGMIETDFPKVPEIIVFQAETSDVAWIPREGEIDRFYVRMDTKDFTLQEAMDK
INHAVRPHSLNFKNIVWFSQFSVKESVAERFFIQNRIFLAGDSCHIHSVNGGQGLNTGLADAFNLMWKLHMVMHFGAPKE
LLHSYEAERKPVAHGVIETSGELVRSTKYSLNGTHAQDYVKIVQKRAGYITGMGIRYGETGLCGSRLFDFEIFNGIAKTR
LYSLLDYMKFTLFIFGDREVELQPPEFVKIIPIYPNQCQENFWTNNTHYANQAILVRPDSYIQYAVPLDKIESLLELAQL
>Mature_479_residues
SAQVADVVIVGAGPVGLMCAYLGQLCGIHTVIVDKSDGPLEVGRADALNARTLQLFELVDLFDELYPLGKTCNTSSVWAD
GQFISRQSSWWEELEGCLHKHFLMLGQSYVEKLLDDKLKETVAAVKRSTAIVNIEINEAGCLTTLANGERIQSRYVIGAD
GARSFVRNHFAIPFEIIRPQIIWAVIDGMIETDFPKVPEIIVFQAETSDVAWIPREGEIDRFYVRMDTKDFTLQEAMDKI
NHAVRPHSLNFKNIVWFSQFSVKESVAERFFIQNRIFLAGDSCHIHSVNGGQGLNTGLADAFNLMWKLHMVMHFGAPKEL
LHSYEAERKPVAHGVIETSGELVRSTKYSLNGTHAQDYVKIVQKRAGYITGMGIRYGETGLCGSRLFDFEIFNGIAKTRL
YSLLDYMKFTLFIFGDREVELQPPEFVKIIPIYPNQCQENFWTNNTHYANQAILVRPDSYIQYAVPLDKIESLLELAQL

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=347, Percent_Identity=28.5302593659942, Blast_Score=120, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 54219; Mature: 54088

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAQVADVVIVGAGPVGLMCAYLGQLCGIHTVIVDKSDGPLEVGRADALNARTLQLFELV
CCCCEEEEEEECCCHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHH
DLFDELYPLGKTCNTSSVWADGQFISRQSSWWEELEGCLHKHFLMLGQSYVEKLLDDKLK
HHHHHHHCCCCCCCCCCEECCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETVAAVKRSTAIVNIEINEAGCLTTLANGERIQSRYVIGADGARSFVRNHFAIPFEIIRP
HHHHHHHHCEEEEEEEECCCCCEEEECCCCEECCEEEEECCHHHHHHHCCCCCCHHHHHH
QIIWAVIDGMIETDFPKVPEIIVFQAETSDVAWIPREGEIDRFYVRMDTKDFTLQEAMDK
HHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEECCCCCCCEEEEEEECCCCCHHHHHHHH
INHAVRPHSLNFKNIVWFSQFSVKESVAERFFIQNRIFLAGDSCHIHSVNGGQGLNTGLA
HHHCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHCEEEEECCCEEEEECCCCCCCCCHHH
DAFNLMWKLHMVMHFGAPKELLHSYEAERKPVAHGVIETSGELVRSTKYSLNGTHAQDYV
HHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHCCCHHHHHHCCCCCCCCHHHHH
KIVQKRAGYITGMGIRYGETGLCGSRLFDFEIFNGIAKTRLYSLLDYMKFTLFIFGDREV
HHHHHHCCEEEECCEECCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEE
ELQPPEFVKIIPIYPNQCQENFWTNNTHYANQAILVRPDSYIQYAVPLDKIESLLELAQL
EECCCCEEEEEECCCCCHHHCCCCCCCEECCEEEEECCCCCEEEECCHHHHHHHHHHHCC
>Mature Secondary Structure 
SAQVADVVIVGAGPVGLMCAYLGQLCGIHTVIVDKSDGPLEVGRADALNARTLQLFELV
CCCEEEEEEECCCHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHH
DLFDELYPLGKTCNTSSVWADGQFISRQSSWWEELEGCLHKHFLMLGQSYVEKLLDDKLK
HHHHHHHCCCCCCCCCCEECCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETVAAVKRSTAIVNIEINEAGCLTTLANGERIQSRYVIGADGARSFVRNHFAIPFEIIRP
HHHHHHHHCEEEEEEEECCCCCEEEECCCCEECCEEEEECCHHHHHHHCCCCCCHHHHHH
QIIWAVIDGMIETDFPKVPEIIVFQAETSDVAWIPREGEIDRFYVRMDTKDFTLQEAMDK
HHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEECCCCCCCEEEEEEECCCCCHHHHHHHH
INHAVRPHSLNFKNIVWFSQFSVKESVAERFFIQNRIFLAGDSCHIHSVNGGQGLNTGLA
HHHCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHCEEEEECCCEEEEECCCCCCCCCHHH
DAFNLMWKLHMVMHFGAPKELLHSYEAERKPVAHGVIETSGELVRSTKYSLNGTHAQDYV
HHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHCCCHHHHHHCCCCCCCCHHHHH
KIVQKRAGYITGMGIRYGETGLCGSRLFDFEIFNGIAKTRLYSLLDYMKFTLFIFGDREV
HHHHHHCCEEEECCEECCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEE
ELQPPEFVKIIPIYPNQCQENFWTNNTHYANQAILVRPDSYIQYAVPLDKIESLLELAQL
EECCCCEEEEEECCCCCHHHCCCCCCCEECCEEEEECCCCCEEEECCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-(3-hydroxyphenyl)propionate; NADH; O2 [C]

Specific reaction: 3-(3-hydroxyphenyl)propionate + NADH + O2 = H2O + 3-(2,3-dihydroxyphenyl)propionate + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]