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The map label for this gene is proS

Identifier: 54020489

GI number: 54020489

Start: 475990

End: 477429

Strand: Reverse

Name: proS

Synonym: mhp397

Alternate gene names: 54020489

Gene position: 477429-475990 (Counterclockwise)

Preceding gene: 54020490

Following gene: 54020488

Centisome position: 53.48

GC content: 30.76

Gene sequence:

>1440_bases
ATGAAAAAACCTGAGAAAATAACATCAAGAACAGCCAATTTTGCAAAATGATATGTAGATGTTATTACTCAGGCAGATTT
AATGAATTATGGCCCAATTAAGGGAACAATTTATTTTAAACCTTTAGGTTATAAAATTTGGGAAAATATCGTAAAAATTG
TTAATGCATATTTTGTTAAGCAAAAAATTGAAAATGTTTATTTCCCTCTGTTAATTCCACAGGATTTTATTGAAAAAGAA
AAAAAGCACATTGAAGGTTTTGCTCCTGAATTACTAACCATTACCAAAGTTGGGGATAAAAATTTAGTCGAAAATATTTA
TATAAGACCAACAAGCGAGCTTCTTTTTGCAGATTATTTTAAGGCAGAAATTGCAAAAAATAACATTTTGCCAATTAAAT
TAAACCAGTGAAGTCAAGTTTTACGTTGAGAAAAAACTACTAACCCGTTTTTACGAAATACAGAATTTTTATGACAAGAA
GGTCACACAATCCATGCAAGTAAGGTCGAAGCTGACCAATTTGCTAAAAAAATAGCCCGATTTTACAAGTATTTTTTAGA
AAATTATTTGGCCATTCCGGTTATTTCAGGCCAAAAAACTGAGCGGGAAAAGTTTGCTGGGGCTGTAAATACTTATACAG
TTGAAGCAATGATGCAAAATTTTCGCGCCCTACAGTCAGCTACTGCTCACTTTTTAGGTCAAAATTTTGCAAAAAATTTT
GAAATTTTCTATAAAAATAAAGAAAATAAAAGTCAAATCCCTTTCCAAACCTCTTGAGGACTTTCAACACGGCTAATCGG
GGCAATTGTAATGGTCCATAGCGATGATAACGGACTTATTTTCCCACCTAAAATTGCTCCAATTCAGGTTGATATTCTTG
AATTTTTTTCTAAAAAAAATCAAGAAGTTAAAATTTTTGCAAAGAAAATCGCAAAAATTTTAAAAAATGCTAAAATTTCT
TATAAAATTGATGACACGGATCAACAAATCGGATACAAAATTAATAATTCAGAGGTTCACGGTGCACCAATTCGAATTGA
AATTGGTCCTAATGAGGTCAAAAATAATCAAATTTGCTTAGTTCGGCGTGATAACCACCAAAAATTTTTTTTCAATATTG
ATCTGTTAAAGGAAAAATGCCGTAAAATTCTTGAACAAATTCAAGCTGATTTATTCAAAAAAGCAAAAATTCGACTTCTT
GAAAATACAGTTTTTGTAAATTCTATTAATGAATTTGAGCATGCAGTAAAAAATAATAAATTTGTAATTGCCCCTTTTAG
TGAATCTCCCGAAAGAGAGCAAGAAATTCAGGAAAAAACGGGAGCTACTGCCCGCTGCATCCTTCCCAAAAATTCATTTT
TTGCCTTACCACAGACAGGAAATTCAATTTTTAGTGGCGAAAAAACAAATAAATTTGTTTTGTTTGCAAAATCTTACTAA

Upstream 100 bases:

>100_bases
AGGCAACGGTTTTTGGTACCGTCATTCGTTAGTTCGAATCTAACTGGGGCAGCCATATGTTAATTCACCACACCCAAAAA
GTTATAAAAAAAACAAGTTT

Downstream 100 bases:

>100_bases
ATTTTTAATTAAATTTTTTTCTAAAAATTCAAAAAATGTAGTAAAATTAAATAAAAAAGGAGAAATATGCCAATTTTTGA
CATCCATTCAACTGAGGATA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MKKPEKITSRTANFAKWYVDVITQADLMNYGPIKGTIYFKPLGYKIWENIVKIVNAYFVKQKIENVYFPLLIPQDFIEKE
KKHIEGFAPELLTITKVGDKNLVENIYIRPTSELLFADYFKAEIAKNNILPIKLNQWSQVLRWEKTTNPFLRNTEFLWQE
GHTIHASKVEADQFAKKIARFYKYFLENYLAIPVISGQKTEREKFAGAVNTYTVEAMMQNFRALQSATAHFLGQNFAKNF
EIFYKNKENKSQIPFQTSWGLSTRLIGAIVMVHSDDNGLIFPPKIAPIQVDILEFFSKKNQEVKIFAKKIAKILKNAKIS
YKIDDTDQQIGYKINNSEVHGAPIRIEIGPNEVKNNQICLVRRDNHQKFFFNIDLLKEKCRKILEQIQADLFKKAKIRLL
ENTVFVNSINEFEHAVKNNKFVIAPFSESPEREQEIQEKTGATARCILPKNSFFALPQTGNSIFSGEKTNKFVLFAKSY

Sequences:

>Translated_479_residues
MKKPEKITSRTANFAK*YVDVITQADLMNYGPIKGTIYFKPLGYKIWENIVKIVNAYFVKQKIENVYFPLLIPQDFIEKE
KKHIEGFAPELLTITKVGDKNLVENIYIRPTSELLFADYFKAEIAKNNILPIKLNQ*SQVLR*EKTTNPFLRNTEFL*QE
GHTIHASKVEADQFAKKIARFYKYFLENYLAIPVISGQKTEREKFAGAVNTYTVEAMMQNFRALQSATAHFLGQNFAKNF
EIFYKNKENKSQIPFQTS*GLSTRLIGAIVMVHSDDNGLIFPPKIAPIQVDILEFFSKKNQEVKIFAKKIAKILKNAKIS
YKIDDTDQQIGYKINNSEVHGAPIRIEIGPNEVKNNQICLVRRDNHQKFFFNIDLLKEKCRKILEQIQADLFKKAKIRLL
ENTVFVNSINEFEHAVKNNKFVIAPFSESPEREQEIQEKTGATARCILPKNSFFALPQTGNSIFSGEKTNKFVLFAKSY
>Mature_479_residues
MKKPEKITSRTANFAK*YVDVITQADLMNYGPIKGTIYFKPLGYKIWENIVKIVNAYFVKQKIENVYFPLLIPQDFIEKE
KKHIEGFAPELLTITKVGDKNLVENIYIRPTSELLFADYFKAEIAKNNILPIKLNQ*SQVLR*EKTTNPFLRNTEFL*QE
GHTIHASKVEADQFAKKIARFYKYFLENYLAIPVISGQKTEREKFAGAVNTYTVEAMMQNFRALQSATAHFLGQNFAKNF
EIFYKNKENKSQIPFQTS*GLSTRLIGAIVMVHSDDNGLIFPPKIAPIQVDILEFFSKKNQEVKIFAKKIAKILKNAKIS
YKIDDTDQQIGYKINNSEVHGAPIRIEIGPNEVKNNQICLVRRDNHQKFFFNIDLLKEKCRKILEQIQADLFKKAKIRLL
ENTVFVNSINEFEHAVKNNKFVIAPFSESPEREQEIQEKTGATARCILPKNSFFALPQTGNSIFSGEKTNKFVLFAKSY

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro) [H]

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI62241042, Length=487, Percent_Identity=36.9609856262834, Blast_Score=285, Evalue=6e-77,
Organism=Caenorhabditis elegans, GI71991342, Length=491, Percent_Identity=34.826883910387, Blast_Score=284, Evalue=9e-77,
Organism=Caenorhabditis elegans, GI71991346, Length=357, Percent_Identity=39.4957983193277, Blast_Score=251, Evalue=4e-67,
Organism=Saccharomyces cerevisiae, GI6321808, Length=436, Percent_Identity=37.8440366972477, Blast_Score=291, Evalue=2e-79,
Organism=Drosophila melanogaster, GI24649468, Length=488, Percent_Identity=34.0163934426229, Blast_Score=261, Evalue=5e-70,
Organism=Drosophila melanogaster, GI24649466, Length=488, Percent_Identity=34.0163934426229, Blast_Score=261, Evalue=6e-70,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004499
- InterPro:   IPR017449
- InterPro:   IPR016061 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF09180 ProRS-C_1; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 54508; Mature: 54508

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKPEKITSRTANFAKYVDVITQADLMNYGPIKGTIYFKPLGYKIWENIVKIVNAYFVKQ
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHH
KIENVYFPLLIPQDFIEKEKKHIEGFAPELLTITKVGDKNLVENIYIRPTSELLFADYFK
HHHHEEEEEECCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHCEEEECCCHHHHHHHHHH
AEIAKNNILPIKLNQSQVLREKTTNPFLRNTEFLQEGHTIHASKVEADQFAKKIARFYKY
HHHHCCCEEEEEECHHHHHHHHCCCCCCCCCHHHHCCCEEEECCCCHHHHHHHHHHHHHH
FLENYLAIPVISGQKTEREKFAGAVNTYTVEAMMQNFRALQSATAHFLGQNFAKNFEIFY
HHHCCEEEEEECCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
KNKENKSQIPFQTSGLSTRLIGAIVMVHSDDNGLIFPPKIAPIQVDILEFFSKKNQEVKI
ECCCCCCCCCEECCCCCCEEEEEEEEEEECCCCEEECCCCCCEEHHHHHHHCCCCCHHHH
FAKKIAKILKNAKISYKIDDTDQQIGYKINNSEVHGAPIRIEIGPNEVKNNQICLVRRDN
HHHHHHHHHHCCEEEEEECCCCHHCCEEECCCEECCCEEEEEECCCCCCCCEEEEEEECC
HQKFFFNIDLLKEKCRKILEQIQADLFKKAKIRLLENTVFVNSINEFEHAVKNNKFVIAP
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECHHHHHHHHCCCCEEEEE
FSESPEREQEIQEKTGATARCILPKNSFFALPQTGNSIFSGEKTNKFVLFAKSY
CCCCCHHHHHHHHHCCCEEEEEECCCCEEECCCCCCCCCCCCCCCEEEEEEECC
>Mature Secondary Structure
MKKPEKITSRTANFAKYVDVITQADLMNYGPIKGTIYFKPLGYKIWENIVKIVNAYFVKQ
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHH
KIENVYFPLLIPQDFIEKEKKHIEGFAPELLTITKVGDKNLVENIYIRPTSELLFADYFK
HHHHEEEEEECCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHCEEEECCCHHHHHHHHHH
AEIAKNNILPIKLNQSQVLREKTTNPFLRNTEFLQEGHTIHASKVEADQFAKKIARFYKY
HHHHCCCEEEEEECHHHHHHHHCCCCCCCCCHHHHCCCEEEECCCCHHHHHHHHHHHHHH
FLENYLAIPVISGQKTEREKFAGAVNTYTVEAMMQNFRALQSATAHFLGQNFAKNFEIFY
HHHCCEEEEEECCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
KNKENKSQIPFQTSGLSTRLIGAIVMVHSDDNGLIFPPKIAPIQVDILEFFSKKNQEVKI
ECCCCCCCCCEECCCCCCEEEEEEEEEEECCCCEEECCCCCCEEHHHHHHHCCCCCHHHH
FAKKIAKILKNAKISYKIDDTDQQIGYKINNSEVHGAPIRIEIGPNEVKNNQICLVRRDN
HHHHHHHHHHCCEEEEEECCCCHHCCEEECCCEECCCEEEEEECCCCCCCCEEEEEEECC
HQKFFFNIDLLKEKCRKILEQIQADLFKKAKIRLLENTVFVNSINEFEHAVKNNKFVIAP
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECHHHHHHHHCCCCEEEEE
FSESPEREQEIQEKTGATARCILPKNSFFALPQTGNSIFSGEKTNKFVLFAKSY
CCCCCHHHHHHHHHCCCEEEEEECCCCEEECCCCCCCCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA