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The map label for this gene is bstVIM [H]

Identifier: 54020460

GI number: 54020460

Start: 397129

End: 398550

Strand: Reverse

Name: bstVIM [H]

Synonym: mhp340

Alternate gene names: 54020460

Gene position: 398550-397129 (Counterclockwise)

Preceding gene: 54020461

Following gene: 54020459

Centisome position: 44.64

GC content: 25.46

Gene sequence:

>1422_bases
ATGCATGAGAATGTTAGTCAAAAATTAAATGGGCAAGTTTTTACACCAGATTTATTGGTTGACCTTATTTTAGATCAAGC
TGGTTACAAAGAAAACATACTTAAAAAACATATTATTGATAACAGTTGTGGTAATGGTCAATTTTTAGTTAAAATTATTG
AACGTTATTGCAAAGCTTTTTTTAGAGAAAATTCGAATTTAAAATCGCTTAAACATCAATTAGAAACTTATATTCATGGC
ATTGATATTGAAGAAAAACATATAAAAAATGCCATTTTCCGTGCAAATTTAGTTGTTCAAAATTATAAAATTGAAGGAGT
AAACTGAGATTTTAAAGCTAAAAATACCCTTGAAATTGAACATTTTAACGGAAAAATGGATTTTGTTGTCGGAAATCCAC
CTTATATTCGCATTCATAATTTAAATAATAATAAATTGTTGAAAAATTTTAATTTTCCAACAATTGGTATGACAGATATT
TACCTTGCTTTTTATGAAATTGGTATTAAAATGTTAAGTAAAAATGGAATTTTATCTTATATTAGTCCATCTTCATTTTT
TACATCCAAAGCAGGCTCAATTTTTCGGGAATTTTTATACAAAAATAAAATCATAAAATCAGTTGTTGATCATAAACATC
ACCAATTTTTTAAAGCAACAACTTATACAACGATTTTCACAATTGATAAATCAGCTAGAAAACAAGTGGTCGATTATTTT
AATTTTGATAATAAAACAAATTCAATTTTTCTTGTTTCTAAATTAGAATATGAACAATTTTATTTAGATAAGAATTTTTA
TTTTTCAACTCCAGAAAAGCTATCTTTTCTAAAGGAAATTATTTACAACAAGAAAAAAAGTGATATTAGTGTCAAAAATG
GTTTGGCAACCTTGGCTGATTCAGTTTTTATTGGCGATTTTAAATTTAATTCTAAATATATTTTGCCCGTTTTAAAAGCA
TCAAATGGAAAATGGGCAAAAATCATTTTTCCGTATAATACCAAAAATTTCCAAATTATTTCAGATTCTGAATTAAAACA
GCAGCTAGAAATTTTTGAACACCTTAGTTCTTTTAAGGAAAAACTGCAAAAACGTAGCTTTGACCATTCAAATAAAAATT
TTTGGTATAGTTTTGGTCGAAGACAAGCTATTTCAGACACTGATAAAGAAAGACTGGTTTTAAATTCATTAGTCAAGGAA
AACAGACCTTTAAAAATAAGTTTAATCCAAAAAAACACCTGTATTTATAGTGGTTTTTACATTATAAGCGAGAAAATTGA
CTATAAATCAATTGCTGAAAAATTACAAAGTAAAGTTTTTCTTGACTTTGTAGAATTATTAGGAAAGTACAAAAATGGTG
GTTATTATACTTTTTCAACAAAAGATGTTAAAAAATATTTAGACTTTATGTTAGGGACGTAA

Upstream 100 bases:

>100_bases
TTTTTATCAATGATTTTGAATGCATAACTTTTGCTAATTTTTGGTATAATTTTATATTAACTTCGTAATTTAAAATAAAA
TAAGAAATAAAAGTTTATTT

Downstream 100 bases:

>100_bases
ATGAAAAAATTAAAGAAAGTTTTATAACTTATCTTCATAAAGGTTCGTCAAGATCAACTGAAAAAATAAAGATAATTCAT
AGTTTTGTTGCTAGTTCAAT

Product: hypothetical protein

Products: NA

Alternate protein names: M.BstVI; Adenine-specific methyltransferase BstVI [H]

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MHENVSQKLNGQVFTPDLLVDLILDQAGYKENILKKHIIDNSCGNGQFLVKIIERYCKAFFRENSNLKSLKHQLETYIHG
IDIEEKHIKNAIFRANLVVQNYKIEGVNWDFKAKNTLEIEHFNGKMDFVVGNPPYIRIHNLNNNKLLKNFNFPTIGMTDI
YLAFYEIGIKMLSKNGILSYISPSSFFTSKAGSIFREFLYKNKIIKSVVDHKHHQFFKATTYTTIFTIDKSARKQVVDYF
NFDNKTNSIFLVSKLEYEQFYLDKNFYFSTPEKLSFLKEIIYNKKKSDISVKNGLATLADSVFIGDFKFNSKYILPVLKA
SNGKWAKIIFPYNTKNFQIISDSELKQQLEIFEHLSSFKEKLQKRSFDHSNKNFWYSFGRRQAISDTDKERLVLNSLVKE
NRPLKISLIQKNTCIYSGFYIISEKIDYKSIAEKLQSKVFLDFVELLGKYKNGGYYTFSTKDVKKYLDFMLGT

Sequences:

>Translated_473_residues
MHENVSQKLNGQVFTPDLLVDLILDQAGYKENILKKHIIDNSCGNGQFLVKIIERYCKAFFRENSNLKSLKHQLETYIHG
IDIEEKHIKNAIFRANLVVQNYKIEGVN*DFKAKNTLEIEHFNGKMDFVVGNPPYIRIHNLNNNKLLKNFNFPTIGMTDI
YLAFYEIGIKMLSKNGILSYISPSSFFTSKAGSIFREFLYKNKIIKSVVDHKHHQFFKATTYTTIFTIDKSARKQVVDYF
NFDNKTNSIFLVSKLEYEQFYLDKNFYFSTPEKLSFLKEIIYNKKKSDISVKNGLATLADSVFIGDFKFNSKYILPVLKA
SNGKWAKIIFPYNTKNFQIISDSELKQQLEIFEHLSSFKEKLQKRSFDHSNKNFWYSFGRRQAISDTDKERLVLNSLVKE
NRPLKISLIQKNTCIYSGFYIISEKIDYKSIAEKLQSKVFLDFVELLGKYKNGGYYTFSTKDVKKYLDFMLGT
>Mature_473_residues
MHENVSQKLNGQVFTPDLLVDLILDQAGYKENILKKHIIDNSCGNGQFLVKIIERYCKAFFRENSNLKSLKHQLETYIHG
IDIEEKHIKNAIFRANLVVQNYKIEGVN*DFKAKNTLEIEHFNGKMDFVVGNPPYIRIHNLNNNKLLKNFNFPTIGMTDI
YLAFYEIGIKMLSKNGILSYISPSSFFTSKAGSIFREFLYKNKIIKSVVDHKHHQFFKATTYTTIFTIDKSARKQVVDYF
NFDNKTNSIFLVSKLEYEQFYLDKNFYFSTPEKLSFLKEIIYNKKKSDISVKNGLATLADSVFIGDFKFNSKYILPVLKA
SNGKWAKIIFPYNTKNFQIISDSELKQQLEIFEHLSSFKEKLQKRSFDHSNKNFWYSFGRRQAISDTDKERLVLNSLVKE
NRPLKISLIQKNTCIYSGFYIISEKIDYKSIAEKLQSKVFLDFVELLGKYKNGGYYTFSTKDVKKYLDFMLGT

Specific function: This methylase recognizes the double-stranded sequence CTCGAG, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the BstVI endonuclease [H]

COG id: COG1002

COG function: function code V; Type II restriction enzyme, methylase subunits

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR002296
- InterPro:   IPR011639 [H]

Pfam domain/function: PF07669 Eco57I [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 55128; Mature: 55128

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHENVSQKLNGQVFTPDLLVDLILDQAGYKENILKKHIIDNSCGNGQFLVKIIERYCKAF
CCCCHHHHHCCCEECHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
FRENSNLKSLKHQLETYIHGIDIEEKHIKNAIFRANLVVQNYKIEGVNDFKAKNTLEIEH
HHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHEEEEEEEEEECCCCCCCCCCEEEEEE
FNGKMDFVVGNPPYIRIHNLNNNKLLKNFNFPTIGMTDIYLAFYEIGIKMLSKNGILSYI
ECCEEEEEECCCCEEEEEECCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEE
SPSSFFTSKAGSIFREFLYKNKIIKSVVDHKHHQFFKATTYTTIFTIDKSARKQVVDYFN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEEECHHHHHHHHHHHC
FDNKTNSIFLVSKLEYEQFYLDKNFYFSTPEKLSFLKEIIYNKKKSDISVKNGLATLADS
CCCCCCEEEEEEEECHHHEEECCCCEECCHHHHHHHHHHHHCCCCCCCCHHCCHHHHHCC
VFIGDFKFNSKYILPVLKASNGKWAKIIFPYNTKNFQIISDSELKQQLEIFEHLSSFKEK
EEEEEEEECCEEEEEEEECCCCCEEEEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHH
LQKRSFDHSNKNFWYSFGRRQAISDTDKERLVLNSLVKENRPLKISLIQKNTCIYSGFYI
HHHCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCEEEEEEEE
ISEKIDYKSIAEKLQSKVFLDFVELLGKYKNGGYYTFSTKDVKKYLDFMLGT
EECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHCCC
>Mature Secondary Structure
MHENVSQKLNGQVFTPDLLVDLILDQAGYKENILKKHIIDNSCGNGQFLVKIIERYCKAF
CCCCHHHHHCCCEECHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
FRENSNLKSLKHQLETYIHGIDIEEKHIKNAIFRANLVVQNYKIEGVNDFKAKNTLEIEH
HHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHEEEEEEEEEECCCCCCCCCCEEEEEE
FNGKMDFVVGNPPYIRIHNLNNNKLLKNFNFPTIGMTDIYLAFYEIGIKMLSKNGILSYI
ECCEEEEEECCCCEEEEEECCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEE
SPSSFFTSKAGSIFREFLYKNKIIKSVVDHKHHQFFKATTYTTIFTIDKSARKQVVDYFN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEEECHHHHHHHHHHHC
FDNKTNSIFLVSKLEYEQFYLDKNFYFSTPEKLSFLKEIIYNKKKSDISVKNGLATLADS
CCCCCCEEEEEEEECHHHEEECCCCEECCHHHHHHHHHHHHCCCCCCCCHHCCHHHHHCC
VFIGDFKFNSKYILPVLKASNGKWAKIIFPYNTKNFQIISDSELKQQLEIFEHLSSFKEK
EEEEEEEECCEEEEEEEECCCCCEEEEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHH
LQKRSFDHSNKNFWYSFGRRQAISDTDKERLVLNSLVKENRPLKISLIQKNTCIYSGFYI
HHHCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCEEEEEEEE
ISEKIDYKSIAEKLQSKVFLDFVELLGKYKNGGYYTFSTKDVKKYLDFMLGT
EECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8370531 [H]