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The map label for this gene is tpiA

Identifier: 54020428

GI number: 54020428

Start: 309630

End: 310358

Strand: Reverse

Name: tpiA

Synonym: mhp277

Alternate gene names: 54020428

Gene position: 310358-309630 (Counterclockwise)

Preceding gene: 54020429

Following gene: 54020427

Centisome position: 34.76

GC content: 31.28

Gene sequence:

>729_bases
ATGAAAAAAATTATTATTGGAAATTGAAAAATGAATAAAACCGTAAGTGAAACACGTGATTTTATTCAAAAATTTGACAT
TTTCTATCAGGAAAATGTGGGCAAAATCAAAGAAGATTTAGATTTTGCAATAGCTCCAAGTTTTATATCTTTATCACTAA
TTTCTAAGTCCTTGACTAAAAAATTAGAAATTGCTGCTCAAAATCTTAGTCAGTTTGATTCAGGAGCCTTTACTGGGGAA
ATCAGTGGCAAAATGCTGCAGGATTTAGGGACAAAATATGTAATTATTGGGCATTCTGAAAGAAGAGAAATTTTTAAAGA
AAAAGATGAAGAACTAAAAAATAAAATTTTACAAGCACAAAAATATGATTTAATTCCTGTTTTTTGTGTTGGTGAAAGTC
TTTTAGAATTTGAAGCCGGCCTAACTAAAAAAGTGATAATTTCGCAGATAAATGCTATAAAATCAGTGCTAAATTTTCAA
AAGGCAATTATTGCATATGAACCAATTTGGGCCATTGGAACTGGCAAAACAGCAACGGCTGCAATAGCAGAAAAAGTTTG
TGGACTGATTAAGGAAAATTTTGGGAAAAATACAATGGTAATTTATGGAGGCTCTGTTAATTCTAAGAATATTAACGAGT
TAGTATCCCAGAAAAGCATCGATGGCGCCCTTGTAGGTGGAGCCTCTCTTGATCCAGAAGAATTTGGAAAAATTTTAGTT
AATAGTTAA

Upstream 100 bases:

>100_bases
TTTTTTTTCTTAAATGAAAAAATACTTTAATTTAAAGGATAATTTAAATTTTTTACCTTTAAGTTAAACGAAAAAATGAT
ATAATTGATTAATATTTATT

Downstream 100 bases:

>100_bases
TTTTTTACTTTTTTATTGTATAATTTAGAAAGATTTGATTTATTACTTGGTTTTAATAGTTTTCGCGGATTAATACTAGG
TTTTAAATTTTTAATTAAAT

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase [H]

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MKKIIIGNWKMNKTVSETRDFIQKFDIFYQENVGKIKEDLDFAIAPSFISLSLISKSLTKKLEIAAQNLSQFDSGAFTGE
ISGKMLQDLGTKYVIIGHSERREIFKEKDEELKNKILQAQKYDLIPVFCVGESLLEFEAGLTKKVIISQINAIKSVLNFQ
KAIIAYEPIWAIGTGKTATAAIAEKVCGLIKENFGKNTMVIYGGSVNSKNINELVSQKSIDGALVGGASLDPEEFGKILV
NS

Sequences:

>Translated_242_residues
MKKIIIGN*KMNKTVSETRDFIQKFDIFYQENVGKIKEDLDFAIAPSFISLSLISKSLTKKLEIAAQNLSQFDSGAFTGE
ISGKMLQDLGTKYVIIGHSERREIFKEKDEELKNKILQAQKYDLIPVFCVGESLLEFEAGLTKKVIISQINAIKSVLNFQ
KAIIAYEPIWAIGTGKTATAAIAEKVCGLIKENFGKNTMVIYGGSVNSKNINELVSQKSIDGALVGGASLDPEEFGKILV
NS
>Mature_242_residues
MKKIIIGN*KMNKTVSETRDFIQKFDIFYQENVGKIKEDLDFAIAPSFISLSLISKSLTKKLEIAAQNLSQFDSGAFTGE
ISGKMLQDLGTKYVIIGHSERREIFKEKDEELKNKILQAQKYDLIPVFCVGESLLEFEAGLTKKVIISQINAIKSVLNFQ
KAIIAYEPIWAIGTGKTATAAIAEKVCGLIKENFGKNTMVIYGGSVNSKNINELVSQKSIDGALVGGASLDPEEFGKILV
NS

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family [H]

Homologues:

Organism=Homo sapiens, GI226529917, Length=211, Percent_Identity=43.6018957345972, Blast_Score=175, Evalue=3e-44,
Organism=Homo sapiens, GI4507645, Length=211, Percent_Identity=43.6018957345972, Blast_Score=175, Evalue=3e-44,
Organism=Escherichia coli, GI1790353, Length=208, Percent_Identity=44.2307692307692, Blast_Score=168, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI17536593, Length=209, Percent_Identity=43.5406698564593, Blast_Score=158, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6320255, Length=235, Percent_Identity=35.7446808510638, Blast_Score=134, Evalue=2e-32,
Organism=Drosophila melanogaster, GI28572008, Length=185, Percent_Identity=43.2432432432432, Blast_Score=146, Evalue=1e-35,
Organism=Drosophila melanogaster, GI28572006, Length=185, Percent_Identity=43.2432432432432, Blast_Score=146, Evalue=1e-35,
Organism=Drosophila melanogaster, GI28572004, Length=185, Percent_Identity=43.2432432432432, Blast_Score=146, Evalue=1e-35,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861 [H]

Pfam domain/function: PF00121 TIM [H]

EC number: =5.3.1.1 [H]

Molecular weight: Translated: 26503; Mature: 26503

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: PS00171 TIM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIIIGNKMNKTVSETRDFIQKFDIFYQENVGKIKEDLDFAIAPSFISLSLISKSLTKK
CCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCHHHHHHHHHHHHHHHH
LEIAAQNLSQFDSGAFTGEISGKMLQDLGTKYVIIGHSERREIFKEKDEELKNKILQAQK
HHHHHHHHHHHCCCCCCCCCHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHC
YDLIPVFCVGESLLEFEAGLTKKVIISQINAIKSVLNFQKAIIAYEPIWAIGTGKTATAA
CCCEEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHH
IAEKVCGLIKENFGKNTMVIYGGSVNSKNINELVSQKSIDGALVGGASLDPEEFGKILVN
HHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCCEEECCCCCCHHHHHHHHCC
S
C
>Mature Secondary Structure
MKKIIIGNKMNKTVSETRDFIQKFDIFYQENVGKIKEDLDFAIAPSFISLSLISKSLTKK
CCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCHHHHHHHHHHHHHHHH
LEIAAQNLSQFDSGAFTGEISGKMLQDLGTKYVIIGHSERREIFKEKDEELKNKILQAQK
HHHHHHHHHHHCCCCCCCCCHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHC
YDLIPVFCVGESLLEFEAGLTKKVIISQINAIKSVLNFQKAIIAYEPIWAIGTGKTATAA
CCCEEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHH
IAEKVCGLIKENFGKNTMVIYGGSVNSKNINELVSQKSIDGALVGGASLDPEEFGKILVN
HHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCCEEECCCCCCHHHHHHHHCC
S
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA