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The map label for this gene is truB

Identifier: 54020337

GI number: 54020337

Start: 118572

End: 119555

Strand: Reverse

Name: truB

Synonym: mhp104

Alternate gene names: 54020337

Gene position: 119555-118572 (Counterclockwise)

Preceding gene: 54020338

Following gene: 54020336

Centisome position: 13.39

GC content: 29.27

Gene sequence:

>984_bases
ATGGCAGACACGCTAGATTTAGGCTCTAGTGCCTTCGGGTGTAAGGGTTCAAGTCCCTTCTTGCGCACCACGATGATAGG
TCAACCTTGTGTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAAAAAAAAAAAGATGATTACATTTTTATATA
AACCAAAAAAAATTAGCTCCGCAAAATTTCTAAGGCAGTGATCAAAAACTAATTTGATTAAAAAAGCAGGCCATGCAGGG
ACTCTTGATCCATTAGCATCCGGACTTTTATTGGTTGCAACTGAAGATGATACAAAATTATTACAATATTTAGATCAGAA
GACAAAAACTTATTTAGCAAAAATTCAGTTTGGTTTTTGATCAACAACCTATGATGCCGAAGGTCAAATTTACGGGGTTG
AACAGCCGATTAAAGTTACTAAAGACAATTTAGAACAAGCATTAAATAGGTTATCAGAAAGCCAAAAACAGGTTCCTCCA
GTTTTTTCAAGTAAAAAAGTATCTGGAAAATCGGCTTATCATTATGCTCGTCAAGGTAAACAGATTGAATTAAAACCGAT
TAGTATTAAAATTAGTAAAACAATTTTAATAAATTTTGATGAAAAGCTGCAAAATTGTGTTATAATGTGACAAGTTTCTC
GCGGTTGCTATATTCGCTCACTTGCTGATGATTTAGGAAAAATGCTAAAAACTAGAGCATACTTAAGTGATCTTGAAAGA
ACAAAAATTGGGAATTTTGATAAAAAATTTTTAAATCAATCTTTAAAACCTCAAGATTTATTTGATTTGCAGCAGGTAAA
ATTAGATCTTGAAAATTTAGAATTATTATTACAAGGTAAAAAAATTAATTATTTTGCCAAAAACAGCGAGCTAAACACCT
TAATTTTCAAAGATGAAGTTGTTGGTTTTGGTAAAATAATTAATAATGTACTAATTACGAAAAAATTGTTTGGCAACCGT
ATAAAAAAAATAATTAATACTTAA

Upstream 100 bases:

>100_bases
AGTCGCCTTGTAAGCGAAAGGTCGTAGGTTCGATTCCTATAAGTGGCACCAAAAATAAAATTATTTCTTAAACTGCGTGT
ACTTTTGCGCGAGTGGTGAA

Downstream 100 bases:

>100_bases
AATTCACTACAAAATGAATAAATTTAAACTTTTTGCCACAGATATCGATGATACAATAGTGCCCCATGGTGGTCAGATAA
TTCCTGATCAAATCCAGTTA

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MADTLDLGSSAFGCKGSSPFLRTTMIGQPCVDFFFFFFFFFKKKKKKMITFLYKPKKISSAKFLRQWSKTNLIKKAGHAG
TLDPLASGLLLVATEDDTKLLQYLDQKTKTYLAKIQFGFWSTTYDAEGQIYGVEQPIKVTKDNLEQALNRLSESQKQVPP
VFSSKKVSGKSAYHYARQGKQIELKPISIKISKTILINFDEKLQNCVIMWQVSRGCYIRSLADDLGKMLKTRAYLSDLER
TKIGNFDKKFLNQSLKPQDLFDLQQVKLDLENLELLLQGKKINYFAKNSELNTLIFKDEVVGFGKIINNVLITKKLFGNR
IKKIINT

Sequences:

>Translated_327_residues
MADTLDLGSSAFGCKGSSPFLRTTMIGQPCVDFFFFFFFFFKKKKKKMITFLYKPKKISSAKFLRQ*SKTNLIKKAGHAG
TLDPLASGLLLVATEDDTKLLQYLDQKTKTYLAKIQFGF*STTYDAEGQIYGVEQPIKVTKDNLEQALNRLSESQKQVPP
VFSSKKVSGKSAYHYARQGKQIELKPISIKISKTILINFDEKLQNCVIM*QVSRGCYIRSLADDLGKMLKTRAYLSDLER
TKIGNFDKKFLNQSLKPQDLFDLQQVKLDLENLELLLQGKKINYFAKNSELNTLIFKDEVVGFGKIINNVLITKKLFGNR
IKKIINT
>Mature_326_residues
ADTLDLGSSAFGCKGSSPFLRTTMIGQPCVDFFFFFFFFFKKKKKKMITFLYKPKKISSAKFLRQ*SKTNLIKKAGHAGT
LDPLASGLLLVATEDDTKLLQYLDQKTKTYLAKIQFGF*STTYDAEGQIYGVEQPIKVTKDNLEQALNRLSESQKQVPPV
FSSKKVSGKSAYHYARQGKQIELKPISIKISKTILINFDEKLQNCVIM*QVSRGCYIRSLADDLGKMLKTRAYLSDLERT
KIGNFDKKFLNQSLKPQDLFDLQQVKLDLENLELLLQGKKINYFAKNSELNTLIFKDEVVGFGKIINNVLITKKLFGNRI
KKIINT

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=244, Percent_Identity=29.9180327868852, Blast_Score=89, Evalue=4e-18,
Organism=Escherichia coli, GI2367200, Length=199, Percent_Identity=34.1708542713568, Blast_Score=100, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6324037, Length=138, Percent_Identity=37.6811594202899, Blast_Score=76, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780 [H]

Pfam domain/function: PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 36804; Mature: 36672

Theoretical pI: Translated: 10.38; Mature: 10.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADTLDLGSSAFGCKGSSPFLRTTMIGQPCVDFFFFFFFFFKKKKKKMITFLYKPKKISS
CCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHH
AKFLRQSKTNLIKKAGHAGTLDPLASGLLLVATEDDTKLLQYLDQKTKTYLAKIQFGFST
HHHHHHHHHHHHHHCCCCCCHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHEEECCCC
TYDAEGQIYGVEQPIKVTKDNLEQALNRLSESQKQVPPVFSSKKVSGKSAYHYARQGKQI
CCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCEE
ELKPISIKISKTILINFDEKLQNCVIMQVSRGCYIRSLADDLGKMLKTRAYLSDLERTKI
EEEEEEEEEEEEEEEEEHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
GNFDKKFLNQSLKPQDLFDLQQVKLDLENLELLLQGKKINYFAKNSELNTLIFKDEVVGF
CCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHEEEECCEEEEEECCCCCEEEEEHHHHHHH
GKIINNVLITKKLFGNRIKKIINT
HHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ADTLDLGSSAFGCKGSSPFLRTTMIGQPCVDFFFFFFFFFKKKKKKMITFLYKPKKISS
CCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHH
AKFLRQSKTNLIKKAGHAGTLDPLASGLLLVATEDDTKLLQYLDQKTKTYLAKIQFGFST
HHHHHHHHHHHHHHCCCCCCHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHEEECCCC
TYDAEGQIYGVEQPIKVTKDNLEQALNRLSESQKQVPPVFSSKKVSGKSAYHYARQGKQI
CCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCEE
ELKPISIKISKTILINFDEKLQNCVIMQVSRGCYIRSLADDLGKMLKTRAYLSDLERTKI
EEEEEEEEEEEEEEEEEHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
GNFDKKFLNQSLKPQDLFDLQQVKLDLENLELLLQGKKINYFAKNSELNTLIFKDEVVGF
CCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHEEEECCEEEEEECCCCCEEEEEHHHHHHH
GKIINNVLITKKLFGNRIKKIINT
HHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA