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The map label for this gene is pyrG

Identifier: 54020334

GI number: 54020334

Start: 113986

End: 115590

Strand: Reverse

Name: pyrG

Synonym: mhp100

Alternate gene names: 54020334

Gene position: 115590-113986 (Counterclockwise)

Preceding gene: 54020335

Following gene: 54020333

Centisome position: 12.95

GC content: 31.96

Gene sequence:

>1605_bases
ATGGCTAAATTTATTTTCGTTACCGGTGGTGTTTTATCTGGAATTGGTAAGGGCGTATCAGTTGCCTCAGTTGCTAATTT
ATTAAAAAGTTGTGGTTATTCTGTTTATATTCTCAAATTAGATCCGTATCTAAACGTTGATCCTGGTGTTTTATCCCCTT
ATGAACATGGTGAAGTTTTTGTTACTGCCGATGGAGGTGAAACGGATTTAGACTTAGGCCATTATGAAAGATTTGTTGCC
CAAAATTTTTCAAAGGATTCAAATCATACAAGTGGCAAAATTTTACTTTCAATTATTGAAAAAGAACGCAAAGGTTTTTA
TCAAGGAAAAACAGTTCAGATAATTCCACATGTAATCGATGAAATAATTTTAAGAATTAAAAATGTAGCAAAAAAATATA
AAACTGATTTTGTTCTAGTCGAAATAGGCGGAACTGTTGGCGATATGGAATCAAATCCGTTTTATTTTGCAGCATCCCAA
ATGGCATCCGAATCGAATTTTAAGGATGTTTTTTTTATTCATACAACTTACATTCCATTTTTAAATGCATCCGGGGAGTT
TAAAACAAAGCCGGCACAATTTTCAATTGCTAAGTTAAATTCACGCGGAATTCGTGCGAATGCAATTTTTCTTCGCTTAG
AAAATGATAAAATTGACGAAAAAATTGCTAAAAAGGTAGCAAAAAGCGCCTTTTTATCATTAGAAAATATTATAATTATT
CCTAATTTACAAAATATTTATCAACTACCATTATTACTTGATAAAACAGCTTTATTAAAATCAATTTTTTTACATTTTCA
GTTGGAATTTCGCCAACCAAAATTAGAAAAATGAAAAGAATTTACTAATTTATTACTAAAAAAATGGGAAAAAACAGTGA
AAATAGGTGCGCTCGGCAAATATACCGAATTTCTTGATGCTTATAAGTCAATTTTAGAGGCTTTTAAAATTTGTGCTGCG
CACCAAAAAGTTAATCTTGAATTAGAGTTTGTTGATACGGCAGATAAAAATTTTTCTCTGGCCAGACTAAAAAATTTTCA
GGGAATTATTATCCTTCCTGGTTTTGGCTTTCGCGGCTTTGAAAATAAAGTAGAAGCAGCAATTTTTACCTACCAAAATA
ATATACCAACAATTGGAATTTGTCTTGGAATGCAGGTGATGACAGTAGCGATGGCCAGGCTTAACGGAATAAAAAATGCC
CATTCAGCCGAATTTTTAGCAGAAAAACCTGATCAGATAAGCGTTCTTGATTATAATCACCAAGAAGGTTCAAAATTACA
GATTGGCGGGACATTGCGACTCGGTGAATACCAAGTAATTTTTGCAGAAAATTCAAAAATTGCAAAAATTTATGGTAAAA
ATTCGGCTTTTGAACGTCATAGACACCGATTTGAGGTGGTTAAAAAATATTCGGCTAAAATTGAAAATACTGATTTTATC
TTTAGTGGTAAAGATGCAAAAACCGACTTGATTGAAGTATGTGAGTCAAAATCACATATTTTTTATATTGGAGTTCAATA
TCATCCTGAATTTATCACGCGTCCACTCGATCCACACCCACTTTTTAATAGTTTTATAGCAGCTATTATTGAGAATGTTT
ACTAA

Upstream 100 bases:

>100_bases
GAAATTAGTTTATAATATTTTCTTTTGAAATTAATTAAACTTAATTTTTTATGCAAAAAACGCTTAAATTTATAGTATAA
TTTTTAATTTAAAGGAAATT

Downstream 100 bases:

>100_bases
TTTTTTATTTTTTATTTAATTTTAGCTATCCGAAATAGAATTTTTAAATAAATAAACCCCGAAATTAATGAAACTAAACA
AGCCAGAATTACTGGTGAGT

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase [H]

Number of amino acids: Translated: 534; Mature: 533

Protein sequence:

>534_residues
MAKFIFVTGGVLSGIGKGVSVASVANLLKSCGYSVYILKLDPYLNVDPGVLSPYEHGEVFVTADGGETDLDLGHYERFVA
QNFSKDSNHTSGKILLSIIEKERKGFYQGKTVQIIPHVIDEIILRIKNVAKKYKTDFVLVEIGGTVGDMESNPFYFAASQ
MASESNFKDVFFIHTTYIPFLNASGEFKTKPAQFSIAKLNSRGIRANAIFLRLENDKIDEKIAKKVAKSAFLSLENIIII
PNLQNIYQLPLLLDKTALLKSIFLHFQLEFRQPKLEKWKEFTNLLLKKWEKTVKIGALGKYTEFLDAYKSILEAFKICAA
HQKVNLELEFVDTADKNFSLARLKNFQGIIILPGFGFRGFENKVEAAIFTYQNNIPTIGICLGMQVMTVAMARLNGIKNA
HSAEFLAEKPDQISVLDYNHQEGSKLQIGGTLRLGEYQVIFAENSKIAKIYGKNSAFERHRHRFEVVKKYSAKIENTDFI
FSGKDAKTDLIEVCESKSHIFYIGVQYHPEFITRPLDPHPLFNSFIAAIIENVY

Sequences:

>Translated_534_residues
MAKFIFVTGGVLSGIGKGVSVASVANLLKSCGYSVYILKLDPYLNVDPGVLSPYEHGEVFVTADGGETDLDLGHYERFVA
QNFSKDSNHTSGKILLSIIEKERKGFYQGKTVQIIPHVIDEIILRIKNVAKKYKTDFVLVEIGGTVGDMESNPFYFAASQ
MASESNFKDVFFIHTTYIPFLNASGEFKTKPAQFSIAKLNSRGIRANAIFLRLENDKIDEKIAKKVAKSAFLSLENIIII
PNLQNIYQLPLLLDKTALLKSIFLHFQLEFRQPKLEK*KEFTNLLLKKWEKTVKIGALGKYTEFLDAYKSILEAFKICAA
HQKVNLELEFVDTADKNFSLARLKNFQGIIILPGFGFRGFENKVEAAIFTYQNNIPTIGICLGMQVMTVAMARLNGIKNA
HSAEFLAEKPDQISVLDYNHQEGSKLQIGGTLRLGEYQVIFAENSKIAKIYGKNSAFERHRHRFEVVKKYSAKIENTDFI
FSGKDAKTDLIEVCESKSHIFYIGVQYHPEFITRPLDPHPLFNSFIAAIIENVY
>Mature_533_residues
AKFIFVTGGVLSGIGKGVSVASVANLLKSCGYSVYILKLDPYLNVDPGVLSPYEHGEVFVTADGGETDLDLGHYERFVAQ
NFSKDSNHTSGKILLSIIEKERKGFYQGKTVQIIPHVIDEIILRIKNVAKKYKTDFVLVEIGGTVGDMESNPFYFAASQM
ASESNFKDVFFIHTTYIPFLNASGEFKTKPAQFSIAKLNSRGIRANAIFLRLENDKIDEKIAKKVAKSAFLSLENIIIIP
NLQNIYQLPLLLDKTALLKSIFLHFQLEFRQPKLEK*KEFTNLLLKKWEKTVKIGALGKYTEFLDAYKSILEAFKICAAH
QKVNLELEFVDTADKNFSLARLKNFQGIIILPGFGFRGFENKVEAAIFTYQNNIPTIGICLGMQVMTVAMARLNGIKNAH
SAEFLAEKPDQISVLDYNHQEGSKLQIGGTLRLGEYQVIFAENSKIAKIYGKNSAFERHRHRFEVVKKYSAKIENTDFIF
SGKDAKTDLIEVCESKSHIFYIGVQYHPEFITRPLDPHPLFNSFIAAIIENVY

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen [H]

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI148491070, Length=552, Percent_Identity=37.6811594202899, Blast_Score=377, Evalue=1e-104,
Organism=Homo sapiens, GI28559085, Length=561, Percent_Identity=37.2549019607843, Blast_Score=365, Evalue=1e-101,
Organism=Homo sapiens, GI28559083, Length=561, Percent_Identity=37.2549019607843, Blast_Score=365, Evalue=1e-101,
Organism=Homo sapiens, GI221316689, Length=561, Percent_Identity=37.2549019607843, Blast_Score=365, Evalue=1e-101,
Organism=Escherichia coli, GI1789142, Length=539, Percent_Identity=39.5176252319109, Blast_Score=388, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI25148299, Length=600, Percent_Identity=33.6666666666667, Blast_Score=347, Evalue=9e-96,
Organism=Saccharomyces cerevisiae, GI6322563, Length=564, Percent_Identity=38.2978723404255, Blast_Score=363, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6319432, Length=560, Percent_Identity=38.3928571428571, Blast_Score=362, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24664469, Length=560, Percent_Identity=36.6071428571429, Blast_Score=368, Evalue=1e-102,
Organism=Drosophila melanogaster, GI21357815, Length=505, Percent_Identity=33.8613861386139, Blast_Score=293, Evalue=2e-79,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase [H]

EC number: =6.3.4.2 [H]

Molecular weight: Translated: 60036; Mature: 59905

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKFIFVTGGVLSGIGKGVSVASVANLLKSCGYSVYILKLDPYLNVDPGVLSPYEHGEVF
CCEEEEEECHHHHHCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCEEE
VTADGGETDLDLGHYERFVAQNFSKDSNHTSGKILLSIIEKERKGFYQGKTVQIIPHVID
EEECCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEHHHHHH
EIILRIKNVAKKYKTDFVLVEIGGTVGDMESNPFYFAASQMASESNFKDVFFIHTTYIPF
HHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCEEEEEHHHCCCCCCCEEEEEEEEEEEE
LNASGEFKTKPAQFSIAKLNSRGIRANAIFLRLENDKIDEKIAKKVAKSAFLSLENIIII
ECCCCCCCCCCCEEEEEEECCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCEEEE
PNLQNIYQLPLLLDKTALLKSIFLHFQLEFRQPKLEKKEFTNLLLKKWEKTVKIGALGKY
CCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHEEECCCHHH
TEFLDAYKSILEAFKICAAHQKVNLELEFVDTADKNFSLARLKNFQGIIILPGFGFRGFE
HHHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCEEHHHHCCCCCEEEECCCCCCCCC
NKVEAAIFTYQNNIPTIGICLGMQVMTVAMARLNGIKNAHSAEFLAEKPDQISVLDYNHQ
CCEEEEEEEEECCCCEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCEEEEEECCCC
EGSKLQIGGTLRLGEYQVIFAENSKIAKIYGKNSAFERHRHRFEVVKKYSAKIENTDFIF
CCCEEEECEEEEECCEEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCEEEE
SGKDAKTDLIEVCESKSHIFYIGVQYHPEFITRPLDPHPLFNSFIAAIIENVY
ECCCCCHHHHHHHCCCCCEEEEEEEECHHHHCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKFIFVTGGVLSGIGKGVSVASVANLLKSCGYSVYILKLDPYLNVDPGVLSPYEHGEVF
CEEEEEECHHHHHCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCEEE
VTADGGETDLDLGHYERFVAQNFSKDSNHTSGKILLSIIEKERKGFYQGKTVQIIPHVID
EEECCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEHHHHHH
EIILRIKNVAKKYKTDFVLVEIGGTVGDMESNPFYFAASQMASESNFKDVFFIHTTYIPF
HHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCEEEEEHHHCCCCCCCEEEEEEEEEEEE
LNASGEFKTKPAQFSIAKLNSRGIRANAIFLRLENDKIDEKIAKKVAKSAFLSLENIIII
ECCCCCCCCCCCEEEEEEECCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCEEEE
PNLQNIYQLPLLLDKTALLKSIFLHFQLEFRQPKLEKKEFTNLLLKKWEKTVKIGALGKY
CCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHEEECCCHHH
TEFLDAYKSILEAFKICAAHQKVNLELEFVDTADKNFSLARLKNFQGIIILPGFGFRGFE
HHHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCEEHHHHCCCCCEEEECCCCCCCCC
NKVEAAIFTYQNNIPTIGICLGMQVMTVAMARLNGIKNAHSAEFLAEKPDQISVLDYNHQ
CCEEEEEEEEECCCCEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCEEEEEECCCC
EGSKLQIGGTLRLGEYQVIFAENSKIAKIYGKNSAFERHRHRFEVVKKYSAKIENTDFIF
CCCEEEECEEEEECCEEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCEEEE
SGKDAKTDLIEVCESKSHIFYIGVQYHPEFITRPLDPHPLFNSFIAAIIENVY
ECCCCCHHHHHHHCCCCCEEEEEEEECHHHHCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11353084 [H]