Click here to switch to the map view.

The map label for this gene is cls

Identifier: 54020323

GI number: 54020323

Start: 51969

End: 53495

Strand: Reverse

Name: cls

Synonym: mhp040

Alternate gene names: 54020323

Gene position: 53495-51969 (Counterclockwise)

Preceding gene: 54020324

Following gene: 54020322

Centisome position: 5.99

GC content: 23.71

Gene sequence:

>1527_bases
ATGCATAAATTTATAAAATGATTTTTTTACTATTGTTTCTTAGTAATATTTTTAGTTGCTTTTAGTGCCACAATTTATTT
AATTCATTATTTTTTAGCAAAGAATATGAATTGAATTTCAATTTTGACTATTTTTATAGTTTATGCTTCTACTTCTTTTT
TTAATTTATTTATATTATTACAAAAAAGAAGGTATGAAACGAAAATTTCTTGATTAATTGCTTGTTCAATTTTGCCAATA
ATAGGCCCAATTTCATATATTTTTTTAGGCAGAAAATACTTAAAAAGTCAAAATATTAAAAAATATTTTGCACAATACAG
AAATTTTACTAACCTAAAAGAAAAAGTTCAAACCTGGAAAAATCAAGAATCTAAAGATACTTTACTAAGTTTTTCTAGTG
AATATTTTTCATCCCCAATTAAAGAATTTAATGGAAATTTGCTAGTTGATGGTCATAGTTTTTTTGAAAAACTTTTTGCT
GACATTAAAAATGCAAAAAAATTTATTTTTATTGATGTTTATATAATAAAAAATGATTTTATTTGAAAAAAATTAAAAAA
AATACTGGTTGATAAGCGGAAATGCGGGGTGATAATTAAAATTTTAGTCGATTCTTTTGGCACCTATCTTATAAAAAGGC
GCCATTGAATCGAATTAAAGAAGCAAAAAATTGAGGTTTTGCACTTTAACACCTTTAAAATTCCATTTGTTTCCGGACAA
AGTTTTTACCGAAATCACCGAAAAGTTTATATTATTGACGGAAAAATTGCTTATACAGGCGGAAATAATATATCCGAAGA
ATATTCAAGTTTTGATAAAAATTATGGATATTGAATGGATTTAAACCTTAGACTTGAAGGTGAAATTGTTCAAACTTACT
GTAAAAATTTTATGTTCCATTGAACAAAATGAGGCAAAAAAAAGATATCAAAAGAAAAAATCAATGGATTTTGCAGTATT
AAAAATTCTAATCTAAAGCAAGACAAAAACGATAATCTTGGTGTAGTTATTCAAAATGGACCCAACCTCGAGCACTCATT
AATTGAGGGTTTTATTTTAAAAAAAATTTATTCAGCAAAAAAAAATATAAAAATTTTTACCCCTTATCTTGTTCCCTCGC
AAAAAATTATTGATGCTTTAAAGGATATTTTGCTTGCTAAAATTGAGGTTAATTTTTTCTTACCGGGGAGAAATGATTCA
AAAATTATTAAAACTTTCAATGATTTTTTTGCTAAAAAATTATTGAAATATGGAGCAAAAATACACTATTTTAAAGAACT
TTTCTTCCATGGAAAGTCAATAATCATTGATGATAAATATGGAATGATAGGAACCTCGAATTTAGATTATCGATCGCTTC
TTTTTCAATATGAAACTAATTTATTTTTTAAAGGAAAAATTTTAAGTGACTTTTTAAATTATATTCAGGTACTAAAAAAT
CAAAATATTATTATTGAAATTAATAAGGTATCAAAAGTTTTTTTTATCCTTCGTTTTTTTATATTTTTTTTAAAAACTGT
TATATAA

Upstream 100 bases:

>100_bases
AATAATACCAAATTATTTAATGAAATAAAGGATCAAATTATTGAAAAACTAAAGGAAAATCAACAACAATCCCAAACATT
ATAGTAAAATTATTATTATA

Downstream 100 bases:

>100_bases
AACTGTCTAATTTATAGTATAATTATTAATATTATTATTGGTGTTTTTGAATTATGTGAAGAACAATCATCGTTACGTTT
ATTGCCATTTTTGGTGTTTT

Product: cardiolipin synthetase

Products: NA

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 508; Mature: 508

Protein sequence:

>508_residues
MHKFIKWFFYYCFLVIFLVAFSATIYLIHYFLAKNMNWISILTIFIVYASTSFFNLFILLQKRRYETKISWLIACSILPI
IGPISYIFLGRKYLKSQNIKKYFAQYRNFTNLKEKVQTWKNQESKDTLLSFSSEYFSSPIKEFNGNLLVDGHSFFEKLFA
DIKNAKKFIFIDVYIIKNDFIWKKLKKILVDKRKCGVIIKILVDSFGTYLIKRRHWIELKKQKIEVLHFNTFKIPFVSGQ
SFYRNHRKVYIIDGKIAYTGGNNISEEYSSFDKNYGYWMDLNLRLEGEIVQTYCKNFMFHWTKWGKKKISKEKINGFCSI
KNSNLKQDKNDNLGVVIQNGPNLEHSLIEGFILKKIYSAKKNIKIFTPYLVPSQKIIDALKDILLAKIEVNFFLPGRNDS
KIIKTFNDFFAKKLLKYGAKIHYFKELFFHGKSIIIDDKYGMIGTSNLDYRSLLFQYETNLFFKGKILSDFLNYIQVLKN
QNIIIEINKVSKVFFILRFFIFFLKTVI

Sequences:

>Translated_508_residues
MHKFIK*FFYYCFLVIFLVAFSATIYLIHYFLAKNMN*ISILTIFIVYASTSFFNLFILLQKRRYETKIS*LIACSILPI
IGPISYIFLGRKYLKSQNIKKYFAQYRNFTNLKEKVQTWKNQESKDTLLSFSSEYFSSPIKEFNGNLLVDGHSFFEKLFA
DIKNAKKFIFIDVYIIKNDFI*KKLKKILVDKRKCGVIIKILVDSFGTYLIKRRH*IELKKQKIEVLHFNTFKIPFVSGQ
SFYRNHRKVYIIDGKIAYTGGNNISEEYSSFDKNYGY*MDLNLRLEGEIVQTYCKNFMFH*TK*GKKKISKEKINGFCSI
KNSNLKQDKNDNLGVVIQNGPNLEHSLIEGFILKKIYSAKKNIKIFTPYLVPSQKIIDALKDILLAKIEVNFFLPGRNDS
KIIKTFNDFFAKKLLKYGAKIHYFKELFFHGKSIIIDDKYGMIGTSNLDYRSLLFQYETNLFFKGKILSDFLNYIQVLKN
QNIIIEINKVSKVFFILRFFIFFLKTVI
>Mature_508_residues
MHKFIK*FFYYCFLVIFLVAFSATIYLIHYFLAKNMN*ISILTIFIVYASTSFFNLFILLQKRRYETKIS*LIACSILPI
IGPISYIFLGRKYLKSQNIKKYFAQYRNFTNLKEKVQTWKNQESKDTLLSFSSEYFSSPIKEFNGNLLVDGHSFFEKLFA
DIKNAKKFIFIDVYIIKNDFI*KKLKKILVDKRKCGVIIKILVDSFGTYLIKRRH*IELKKQKIEVLHFNTFKIPFVSGQ
SFYRNHRKVYIIDGKIAYTGGNNISEEYSSFDKNYGY*MDLNLRLEGEIVQTYCKNFMFH*TK*GKKKISKEKINGFCSI
KNSNLKQDKNDNLGVVIQNGPNLEHSLIEGFILKKIYSAKKNIKIFTPYLVPSQKIIDALKDILLAKIEVNFFLPGRNDS
KIIKTFNDFFAKKLLKYGAKIHYFKELFFHGKSIIIDDKYGMIGTSNLDYRSLLFQYETNLFFKGKILSDFLNYIQVLKN
QNIIIEINKVSKVFFILRFFIFFLKTVI

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=452, Percent_Identity=25, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1787007, Length=366, Percent_Identity=24.3169398907104, Blast_Score=104, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR015679
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 58758; Mature: 58758

Theoretical pI: Translated: 10.34; Mature: 10.34

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKFIKFFYYCFLVIFLVAFSATIYLIHYFLAKNMNISILTIFIVYASTSFFNLFILLQK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RRYETKISLIACSILPIIGPISYIFLGRKYLKSQNIKKYFAQYRNFTNLKEKVQTWKNQE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHCCCC
SKDTLLSFSSEYFSSPIKEFNGNLLVDGHSFFEKLFADIKNAKKFIFIDVYIIKNDFIKK
CHHHHHHHHHHHHHCCHHHCCCCEEEECHHHHHHHHHHHHCCCEEEEEEEEEECHHHHHH
LKKILVDKRKCGVIIKILVDSFGTYLIKRRHIELKKQKIEVLHFNTFKIPFVSGQSFYRN
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEEEEEEEECCHHHHCC
HRKVYIIDGKIAYTGGNNISEEYSSFDKNYGYMDLNLRLEGEIVQTYCKNFMFHTKGKKK
CCEEEEEECEEEEECCCCCHHHHHHHCCCCCEEEEEEEECHHHHHHHHHHHEEECCCHHH
ISKEKINGFCSIKNSNLKQDKNDNLGVVIQNGPNLEHSLIEGFILKKIYSAKKNIKIFTP
HHHHHCCCEEEECCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEEC
YLVPSQKIIDALKDILLAKIEVNFFLPGRNDSKIIKTFNDFFAKKLLKYGAKIHYFKELF
CCCCCHHHHHHHHHHHHEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHH
FHGKSIIIDDKYGMIGTSNLDYRSLLFQYETNLFFKGKILSDFLNYIQVLKNQNIIIEIN
HCCCEEEEECCCCCEECCCCCHHHHHHHHHCCEEEEHHHHHHHHHHHHHHCCCCEEEEEH
KVSKVFFILRFFIFFLKTVI
HHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MHKFIKFFYYCFLVIFLVAFSATIYLIHYFLAKNMNISILTIFIVYASTSFFNLFILLQK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RRYETKISLIACSILPIIGPISYIFLGRKYLKSQNIKKYFAQYRNFTNLKEKVQTWKNQE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHCCCC
SKDTLLSFSSEYFSSPIKEFNGNLLVDGHSFFEKLFADIKNAKKFIFIDVYIIKNDFIKK
CHHHHHHHHHHHHHCCHHHCCCCEEEECHHHHHHHHHHHHCCCEEEEEEEEEECHHHHHH
LKKILVDKRKCGVIIKILVDSFGTYLIKRRHIELKKQKIEVLHFNTFKIPFVSGQSFYRN
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEEEEEEEECCHHHHCC
HRKVYIIDGKIAYTGGNNISEEYSSFDKNYGYMDLNLRLEGEIVQTYCKNFMFHTKGKKK
CCEEEEEECEEEEECCCCCHHHHHHHCCCCCEEEEEEEECHHHHHHHHHHHEEECCCHHH
ISKEKINGFCSIKNSNLKQDKNDNLGVVIQNGPNLEHSLIEGFILKKIYSAKKNIKIFTP
HHHHHCCCEEEECCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEEC
YLVPSQKIIDALKDILLAKIEVNFFLPGRNDSKIIKTFNDFFAKKLLKYGAKIHYFKELF
CCCCCHHHHHHHHHHHHEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHH
FHGKSIIIDDKYGMIGTSNLDYRSLLFQYETNLFFKGKILSDFLNYIQVLKNQNIIIEIN
HCCCEEEEECCCCCEECCCCCHHHHHHHHHCCEEEEHHHHHHHHHHHHHHCCCCEEEEEH
KVSKVFFILRFFIFFLKTVI
HHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA