| Definition | Mycoplasma hyopneumoniae 232, complete genome. |
|---|---|
| Accession | NC_006360 |
| Length | 892,758 |
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The map label for this gene is pulA [H]
Identifier: 54020256
GI number: 54020256
Start: 756206
End: 758206
Strand: Direct
Name: pulA [H]
Synonym: mhp605
Alternate gene names: 54020256
Gene position: 756206-758206 (Clockwise)
Preceding gene: 54020255
Following gene: 54020257
Centisome position: 84.7
GC content: 30.13
Gene sequence:
>2001_bases ATGATTTTTAATTATAATACTAGTTTATTCCGTAAGAATTTCCGCGGTGAATCATATTGAGGACCACTTGGTTTAATTTT TAACGGCAATAATTTTGAATTCTACCTTTGATCACCTAATGCAAATCGGGTACATTTTGCAATTTATAAAGATCCGGAAG ACAAAATTCCGGCTGAAATTATTGTGATGTCAAAAAATAATGATGTTTGGTTTTGCCAAATTAATGCCTCTTTTCATGGA TATTCCTATAATTTATTAATTGAGCATCACGATTTAAAAATAACTGAGGCCCTTGATCCTTATGCCTTTAGTATTGCGCC TTTTGATTGAAGAAAAAATGAAAGTCCAAAAGCATATTTAATTGACATTTTTTCCGAAAAAACTGGAAAAAATCCTTCAA AATTAGAAGGATTTAACAAAAATCCGCAAATTGATGCTCAAATTTATCAGCTGCACATTCGAGATTTTTCATCTATTAGT AAAAAAACAGAAAATAAAGGTACTTTTATCGGAGCGCTAGAAAATGATGTTTTTAGTTATTTAAATAGCTTAAAATTTAA TTTTTTACAATTATTACCAATCCACTCTTGTTATAATTTCAGCCAAAAAAACGCTAGCATCCTCCACAAAGGTGATGGAA ACGGTCATTTTAGCACTTATAATTGGGGTTATGACCCAATTGGTTACTTTTCGATAAATTCAAGTTATTCAACAGATCCA ATCGATCCATATCTGCGGATTTTTGAGTTTAAAAAATTTGTTGACTCCGCCCATAAAAATAAGATCGGAATTGTTCTTGA TGTTGATTTTAGTCACACCTTTAAAAATTCAATTCTTGAGGATGTAGCTCACGGGCATTTTTACCGGGATGAAGCAGCTG TTTTACCTGCCGGATTTCCGCCACTTGATACACGAAAACCAATGGCATTTAGGCTAATTTTGGATTCCTTAATTTTTTTT ACTAAATATTATAAAGTTGATGGATTTCGCTTTAATTTAGCATCTTTTTTAGATAAAAAAGCAATTACAGTTATTGCCAG TGAACTAAAAAAAGTTAATCCAAATATTCTTTTATATGGTGATTTTTCAAATTTTAGCGACCTACCAAGCAGAAATCGAC TTGAAAAAGGGAAATCAGGAAATAGTTTTAACTTTGGATATTTAAACGATACAATCCAAACAGCAATTATCGGGAGCGGA AATCCGCGTGATAAAGGTTTAATTTTATCAAAAACTAGTAAAAAATTCGCTGCTTATGTTTCCTCGATTCCGGGAAACAT TGCAAATTTTGACTTCCAAAATTTGCCATATTCAAAAAAAAAATACGATCTTTTTGCAAACGATATCAGCCTAAATCTTG CCTATCTTACTTGTTATAATGGCCCGACTTTAGCCGATAAAATTCTTAGTTCGACAACGCGAATTGGAAAAAGAGAATTT ATTGAAGTCTACCGGCAAGCCTTAATGATGATCAATTTTGTCCAAGGAAAAATTTCACTTAGTGCTGGGACTGAATTTGC TTTTTCAAGAATTTGTGATTTTTCTGGTGGTAGTTATCAAAATTGCTATCCTAATTTAAACATAAAAAGACCGCCTTTTT CGTTTTTAGCGGGCAAATATCTTGATTTTCATTCTGATAAAACCACAGATTTCACAAATGGATTGAATTTTGAAATTCTT AAAAACAATGAAATTAAAGAGAAAATCTTTGATTTTCTTGCCGAGATTAATCAATTTCGCCAAAATTCTCCATTTTTTCG GCTTGATACAAACCAAAAAATCAAAAAGCAGCTAAAATTTGAAACTGTTGATAACAATAAAGGATTAATTATCTTTAAAA TTACTGCTAAAAATAAAGTGATTAAAGTTATTCATAATTTTTCACATCTTTCTTATGAATATGATTTTAAAAATTTTAAT ATTCTTTTTAGCTCAAAGATTAAAGTTATTCCTAATTTAATTCAGAAACATCAGTCGATTTTATTAATTAAAAATAGCTA A
Upstream 100 bases:
>100_bases TATTTTTTATATAATTAGTTTTTTATCGTTAGTAATATAATAATAATAACGATTTTTATGTAAATTTGTAAATTTTAACA TTTTCTAGGCTTATATTAAT
Downstream 100 bases:
>100_bases ATTAACCAAAAAATTTTTATTTAAATTTTCTGGTTAATTTGAAAAATACTTTAATTTATAGATAAATTTAAATATTATAT CTATTTTAGCCAGAATTAAT
Product: pullulanase
Products: NA
Alternate protein names: Alpha-dextrin endo-1,6-alpha-glucosidase; Pullulan 6-glucanohydrolase [H]
Number of amino acids: Translated: 666; Mature: 666
Protein sequence:
>666_residues MIFNYNTSLFRKNFRGESYWGPLGLIFNGNNFEFYLWSPNANRVHFAIYKDPEDKIPAEIIVMSKNNDVWFCQINASFHG YSYNLLIEHHDLKITEALDPYAFSIAPFDWRKNESPKAYLIDIFSEKTGKNPSKLEGFNKNPQIDAQIYQLHIRDFSSIS KKTENKGTFIGALENDVFSYLNSLKFNFLQLLPIHSCYNFSQKNASILHKGDGNGHFSTYNWGYDPIGYFSINSSYSTDP IDPYLRIFEFKKFVDSAHKNKIGIVLDVDFSHTFKNSILEDVAHGHFYRDEAAVLPAGFPPLDTRKPMAFRLILDSLIFF TKYYKVDGFRFNLASFLDKKAITVIASELKKVNPNILLYGDFSNFSDLPSRNRLEKGKSGNSFNFGYLNDTIQTAIIGSG NPRDKGLILSKTSKKFAAYVSSIPGNIANFDFQNLPYSKKKYDLFANDISLNLAYLTCYNGPTLADKILSSTTRIGKREF IEVYRQALMMINFVQGKISLSAGTEFAFSRICDFSGGSYQNCYPNLNIKRPPFSFLAGKYLDFHSDKTTDFTNGLNFEIL KNNEIKEKIFDFLAEINQFRQNSPFFRLDTNQKIKKQLKFETVDNNKGLIIFKITAKNKVIKVIHNFSHLSYEYDFKNFN ILFSSKIKVIPNLIQKHQSILLIKNS
Sequences:
>Translated_666_residues MIFNYNTSLFRKNFRGESY*GPLGLIFNGNNFEFYL*SPNANRVHFAIYKDPEDKIPAEIIVMSKNNDVWFCQINASFHG YSYNLLIEHHDLKITEALDPYAFSIAPFD*RKNESPKAYLIDIFSEKTGKNPSKLEGFNKNPQIDAQIYQLHIRDFSSIS KKTENKGTFIGALENDVFSYLNSLKFNFLQLLPIHSCYNFSQKNASILHKGDGNGHFSTYNWGYDPIGYFSINSSYSTDP IDPYLRIFEFKKFVDSAHKNKIGIVLDVDFSHTFKNSILEDVAHGHFYRDEAAVLPAGFPPLDTRKPMAFRLILDSLIFF TKYYKVDGFRFNLASFLDKKAITVIASELKKVNPNILLYGDFSNFSDLPSRNRLEKGKSGNSFNFGYLNDTIQTAIIGSG NPRDKGLILSKTSKKFAAYVSSIPGNIANFDFQNLPYSKKKYDLFANDISLNLAYLTCYNGPTLADKILSSTTRIGKREF IEVYRQALMMINFVQGKISLSAGTEFAFSRICDFSGGSYQNCYPNLNIKRPPFSFLAGKYLDFHSDKTTDFTNGLNFEIL KNNEIKEKIFDFLAEINQFRQNSPFFRLDTNQKIKKQLKFETVDNNKGLIIFKITAKNKVIKVIHNFSHLSYEYDFKNFN ILFSSKIKVIPNLIQKHQSILLIKNS >Mature_666_residues MIFNYNTSLFRKNFRGESY*GPLGLIFNGNNFEFYL*SPNANRVHFAIYKDPEDKIPAEIIVMSKNNDVWFCQINASFHG YSYNLLIEHHDLKITEALDPYAFSIAPFD*RKNESPKAYLIDIFSEKTGKNPSKLEGFNKNPQIDAQIYQLHIRDFSSIS KKTENKGTFIGALENDVFSYLNSLKFNFLQLLPIHSCYNFSQKNASILHKGDGNGHFSTYNWGYDPIGYFSINSSYSTDP IDPYLRIFEFKKFVDSAHKNKIGIVLDVDFSHTFKNSILEDVAHGHFYRDEAAVLPAGFPPLDTRKPMAFRLILDSLIFF TKYYKVDGFRFNLASFLDKKAITVIASELKKVNPNILLYGDFSNFSDLPSRNRLEKGKSGNSFNFGYLNDTIQTAIIGSG NPRDKGLILSKTSKKFAAYVSSIPGNIANFDFQNLPYSKKKYDLFANDISLNLAYLTCYNGPTLADKILSSTTRIGKREF IEVYRQALMMINFVQGKISLSAGTEFAFSRICDFSGGSYQNCYPNLNIKRPPFSFLAGKYLDFHSDKTTDFTNGLNFEIL KNNEIKEKIFDFLAEINQFRQNSPFFRLDTNQKIKKQLKFETVDNNKGLIIFKITAKNKVIKVIHNFSHLSYEYDFKNFN ILFSSKIKVIPNLIQKHQSILLIKNS
Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]
COG id: COG1523
COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the glycosyl hydrolase 13 family [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR006047 - InterPro: IPR004193 - InterPro: IPR017853 - InterPro: IPR013781 - InterPro: IPR013783 - InterPro: IPR014756 - InterPro: IPR005323 - InterPro: IPR011840 [H]
Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48; PF03714 PUD [H]
EC number: =3.2.1.41 [H]
Molecular weight: Translated: 76009; Mature: 76009
Theoretical pI: Translated: 9.61; Mature: 9.61
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.8 %Cys (Translated Protein) 0.8 %Met (Translated Protein) 1.5 %Cys+Met (Translated Protein) 0.8 %Cys (Mature Protein) 0.8 %Met (Mature Protein) 1.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MIFNYNTSLFRKNFRGESYGPLGLIFNGNNFEFYLSPNANRVHFAIYKDPEDKIPAEIIV CEEECCHHHHHHCCCCCCCCCEEEEEECCCEEEEECCCCCEEEEEEEECCCCCCCEEEEE MSKNNDVWFCQINASFHGYSYNLLIEHHDLKITEALDPYAFSIAPFDRKNESPKAYLIDI EECCCCEEEEEEECCCCCEEEEEEEEECCEEEEECCCCCEEEECCCCCCCCCCCEEEEEE FSEKTGKNPSKLEGFNKNPQIDAQIYQLHIRDFSSISKKTENKGTFIGALENDVFSYLNS ECCCCCCCHHHCCCCCCCCCCCEEEEEEEEHHHHHHHHHCCCCCCEEEEHHHHHHHHHHH LKFNFLQLLPIHSCYNFSQKNASILHKGDGNGHFSTYNWGYDPIGYFSINSSYSTDPIDP HHHHHHEEEEHHHHCCCCCCCCEEEEECCCCCCEEEECCCCCCEEEEEECCCCCCCCCCH YLRIFEFKKFVDSAHKNKIGIVLDVDFSHTFKNSILEDVAHGHFYRDEAAVLPAGFPPLD HHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHCCCEECCCCEECCCCCCCCC TRKPMAFRLILDSLIFFTKYYKVDGFRFNLASFLDKKAITVIASELKKVNPNILLYGDFS CCCCHHHHHHHHHHHHHHHHEEECCEEEEHHHHHCHHHHHHHHHHHHHCCCCEEEEECCC NFSDLPSRNRLEKGKSGNSFNFGYLNDTIQTAIIGSGNPRDKGLILSKTSKKFAAYVSSI CCCCCCCCCHHHCCCCCCCEECCEECCCEEEEEECCCCCCCCCEEEECCCHHHHHHHHHC PGNIANFDFQNLPYSKKKYDLFANDISLNLAYLTCYNGPTLADKILSSTTRIGKREFIEV CCCCCCCCCCCCCCCCCCEEEEECCEEEEEEEEEEECCCHHHHHHHHHHHHCCHHHHHHH YRQALMMINFVQGKISLSAGTEFAFSRICDFSGGSYQNCYPNLNIKRPPFSFLAGKYLDF HHHHHHHHHHHCCEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHCCCEEE HSDKTTDFTNGLNFEILKNNEIKEKIFDFLAEINQFRQNSPFFRLDTNQKIKKQLKFETV CCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHCCEEEE DNNKGLIIFKITAKNKVIKVIHNFSHLSYEYDFKNFNILFSSKIKVIPNLIQKHQSILLI CCCCCEEEEEEECCCCEEHHHHHHHHCEEEECCCCEEEEEECCHHHHHHHHHCCCEEEEE KNS ECC >Mature Secondary Structure MIFNYNTSLFRKNFRGESYGPLGLIFNGNNFEFYLSPNANRVHFAIYKDPEDKIPAEIIV CEEECCHHHHHHCCCCCCCCCEEEEEECCCEEEEECCCCCEEEEEEEECCCCCCCEEEEE MSKNNDVWFCQINASFHGYSYNLLIEHHDLKITEALDPYAFSIAPFDRKNESPKAYLIDI EECCCCEEEEEEECCCCCEEEEEEEEECCEEEEECCCCCEEEECCCCCCCCCCCEEEEEE FSEKTGKNPSKLEGFNKNPQIDAQIYQLHIRDFSSISKKTENKGTFIGALENDVFSYLNS ECCCCCCCHHHCCCCCCCCCCCEEEEEEEEHHHHHHHHHCCCCCCEEEEHHHHHHHHHHH LKFNFLQLLPIHSCYNFSQKNASILHKGDGNGHFSTYNWGYDPIGYFSINSSYSTDPIDP HHHHHHEEEEHHHHCCCCCCCCEEEEECCCCCCEEEECCCCCCEEEEEECCCCCCCCCCH YLRIFEFKKFVDSAHKNKIGIVLDVDFSHTFKNSILEDVAHGHFYRDEAAVLPAGFPPLD HHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHCCCEECCCCEECCCCCCCCC TRKPMAFRLILDSLIFFTKYYKVDGFRFNLASFLDKKAITVIASELKKVNPNILLYGDFS CCCCHHHHHHHHHHHHHHHHEEECCEEEEHHHHHCHHHHHHHHHHHHHCCCCEEEEECCC NFSDLPSRNRLEKGKSGNSFNFGYLNDTIQTAIIGSGNPRDKGLILSKTSKKFAAYVSSI CCCCCCCCCHHHCCCCCCCEECCEECCCEEEEEECCCCCCCCCEEEECCCHHHHHHHHHC PGNIANFDFQNLPYSKKKYDLFANDISLNLAYLTCYNGPTLADKILSSTTRIGKREFIEV CCCCCCCCCCCCCCCCCCEEEEECCEEEEEEEEEEECCCHHHHHHHHHHHHCCHHHHHHH YRQALMMINFVQGKISLSAGTEFAFSRICDFSGGSYQNCYPNLNIKRPPFSFLAGKYLDF HHHHHHHHHHHCCEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHCCCEEE HSDKTTDFTNGLNFEILKNNEIKEKIFDFLAEINQFRQNSPFFRLDTNQKIKKQLKFETV CCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHCCEEEE DNNKGLIIFKITAKNKVIKVIHNFSHLSYEYDFKNFNILFSSKIKVIPNLIQKHQSILLI CCCCCEEEEEEECCCCEEHHHHHHHHCEEEECCCCEEEEEECCHHHHHHHHHCCCEEEEE KNS ECC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9453151; 10360571 [H]