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The map label for this gene is pgk

Identifier: 54020216

GI number: 54020216

Start: 605197

End: 606411

Strand: Direct

Name: pgk

Synonym: mhp488

Alternate gene names: 54020216

Gene position: 605197-606411 (Clockwise)

Preceding gene: 54020215

Following gene: 54020217

Centisome position: 67.79

GC content: 33.99

Gene sequence:

>1215_bases
ATGCAGCCAACCTACAAAAATAAAAAGTTTATTGATGATATTGATTTTTTAAATAAAACTGTAATTCTCCGTGTTGATTT
TAATGTGCCGATCAAAAATGGAGAAATTAGTTCTATAAAAAGAATCATTGCAAGTCTTAAAACTATTAAAAAAATTGTAA
ATAATGGCGGTAAATTAGTGATTTTATCCCATCTTGGCCGGATAAAATCCAAAGAAGATCTTCCAAAAAAATCCCTTAGA
ATAGTAGCTGAAAAATTAGCTGAAATTTTAGGTCAGGAAATTAAATTTATCCCTGAAAACCGTGGTCCTAAAGTCGAAAA
TGCAATTAGCCAACTTGAAAAAGGTGAAATTTTAGTACTTGAAAATACAAGATTTCAGGACCTAAATAATAAAGCTGAGT
CCAAAAACGATGCTGAATTAGGCAGATATTGAGCCTCCCTTGGTGATGTTTTTATCAATGATGCCTTCGGAACTCTTCAT
CGGGCTCATGGATCAAATGTCGGAATTGCAACTTATATTAAAGAATCAGCAATTGGATATTTAGTAAAAGAAGAATTAGA
TGCCCTTTCAAAACTAATTTTTTCCCCGCAAAGACCATTTTATGCAATTATCGGCGGGGCAAAAATATCCGATAAAATCG
GGATAATTTCAACTTTATTAGAAAAAGCTGATAAAGTTTTAATCGGTGGCGGAATGGCTTATACTTTCAAAAAAGCACTT
GGTTATAAAATTGGTCTTTCGATTTATGAAGATGACAAACTTGAACTTGCTGCTAGTCTAGTCAAAAAATATCCAGATAA
ATTAATTTTAGCTCTTGATGCGGCTCTAGCCCCAGAATTTGCCGATCTTGAACCTTTATATAATCAGGAAAATCCCCTTG
AAATCCCTGATCATTTAGAAGGAATGGATATTGGTCCTTTAACAATTGAGTTATTCAAGGATCATCTAAAAGATGGAAAA
ACTATCCTTTGAAATGGAACTTTAGGCGTAGCCGAATTTAAAAATTTTGCCCGTGGAACCAAGGAAGTTGCCAAAATTAT
TGCCAATCTAAAAGAATGTTATAGTATCATTGGCGGGGGAGATTCAATCGCTGCAATTGAAGCCGAAGGTCTTAGTAATT
CATTTTCCCATATTTCTACAGGCGGAGGCGCTTCAATTAGTTTTATTGAAAACGGTGATTTAATTGGACTTGGCCCAATC
CAGGAAAAAGACTAA

Upstream 100 bases:

>100_bases
TTAAAATTGTTAATAATTTTTTTCTTGTTTTTTCTTAGTTTTTCAACTTAATAGTTTATAATATAATAAAAATAAAATTT
TCAAAGTCAAAGGATAAAAA

Downstream 100 bases:

>100_bases
AAGAGCTTTAAATTTAAAGGTTTTTTTTAAATCCCACAAATTTAATAACTAATATTTTTCCTAATTTTTCTAAAAAATAT
TAGTTAATGAAATTTTTTTC

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MQPTYKNKKFIDDIDFLNKTVILRVDFNVPIKNGEISSIKRIIASLKTIKKIVNNGGKLVILSHLGRIKSKEDLPKKSLR
IVAEKLAEILGQEIKFIPENRGPKVENAISQLEKGEILVLENTRFQDLNNKAESKNDAELGRYWASLGDVFINDAFGTLH
RAHGSNVGIATYIKESAIGYLVKEELDALSKLIFSPQRPFYAIIGGAKISDKIGIISTLLEKADKVLIGGGMAYTFKKAL
GYKIGLSIYEDDKLELAASLVKKYPDKLILALDAALAPEFADLEPLYNQENPLEIPDHLEGMDIGPLTIELFKDHLKDGK
TILWNGTLGVAEFKNFARGTKEVAKIIANLKECYSIIGGGDSIAAIEAEGLSNSFSHISTGGGASISFIENGDLIGLGPI
QEKD

Sequences:

>Translated_404_residues
MQPTYKNKKFIDDIDFLNKTVILRVDFNVPIKNGEISSIKRIIASLKTIKKIVNNGGKLVILSHLGRIKSKEDLPKKSLR
IVAEKLAEILGQEIKFIPENRGPKVENAISQLEKGEILVLENTRFQDLNNKAESKNDAELGRY*ASLGDVFINDAFGTLH
RAHGSNVGIATYIKESAIGYLVKEELDALSKLIFSPQRPFYAIIGGAKISDKIGIISTLLEKADKVLIGGGMAYTFKKAL
GYKIGLSIYEDDKLELAASLVKKYPDKLILALDAALAPEFADLEPLYNQENPLEIPDHLEGMDIGPLTIELFKDHLKDGK
TIL*NGTLGVAEFKNFARGTKEVAKIIANLKECYSIIGGGDSIAAIEAEGLSNSFSHISTGGGASISFIENGDLIGLGPI
QEKD
>Mature_404_residues
MQPTYKNKKFIDDIDFLNKTVILRVDFNVPIKNGEISSIKRIIASLKTIKKIVNNGGKLVILSHLGRIKSKEDLPKKSLR
IVAEKLAEILGQEIKFIPENRGPKVENAISQLEKGEILVLENTRFQDLNNKAESKNDAELGRY*ASLGDVFINDAFGTLH
RAHGSNVGIATYIKESAIGYLVKEELDALSKLIFSPQRPFYAIIGGAKISDKIGIISTLLEKADKVLIGGGMAYTFKKAL
GYKIGLSIYEDDKLELAASLVKKYPDKLILALDAALAPEFADLEPLYNQENPLEIPDHLEGMDIGPLTIELFKDHLKDGK
TIL*NGTLGVAEFKNFARGTKEVAKIIANLKECYSIIGGGDSIAAIEAEGLSNSFSHISTGGGASISFIENGDLIGLGPI
QEKD

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI4505763, Length=414, Percent_Identity=38.4057971014493, Blast_Score=254, Evalue=8e-68,
Organism=Homo sapiens, GI31543397, Length=405, Percent_Identity=37.5308641975309, Blast_Score=236, Evalue=4e-62,
Organism=Escherichia coli, GI1789294, Length=394, Percent_Identity=38.3248730964467, Blast_Score=243, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI17508823, Length=416, Percent_Identity=37.9807692307692, Blast_Score=251, Evalue=4e-67,
Organism=Saccharomyces cerevisiae, GI6319857, Length=415, Percent_Identity=39.5180722891566, Blast_Score=250, Evalue=3e-67,
Organism=Drosophila melanogaster, GI17136394, Length=412, Percent_Identity=38.8349514563107, Blast_Score=244, Evalue=6e-65,
Organism=Drosophila melanogaster, GI24581203, Length=412, Percent_Identity=35.4368932038835, Blast_Score=223, Evalue=2e-58,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 43815; Mature: 43815

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPTYKNKKFIDDIDFLNKTVILRVDFNVPIKNGEISSIKRIIASLKTIKKIVNNGGKLV
CCCCCCCCCHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEE
ILSHLGRIKSKEDLPKKSLRIVAEKLAEILGQEIKFIPENRGPKVENAISQLEKGEILVL
EEEHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHCCCCEEEE
ENTRFQDLNNKAESKNDAELGRYASLGDVFINDAFGTLHRAHGSNVGIATYIKESAIGYL
ECCCCHHCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
VKEELDALSKLIFSPQRPFYAIIGGAKISDKIGIISTLLEKADKVLIGGGMAYTFKKALG
HHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCEEEECCCHHHHHHHHHC
YKIGLSIYEDDKLELAASLVKKYPDKLILALDAALAPEFADLEPLYNQENPLEIPDHLEG
CEECCEEECCCHHHHHHHHHHHCCHHEEEHHHHHHCCCCCCCCHHCCCCCCCCCCCCCCC
MDIGPLTIELFKDHLKDGKTILNGTLGVAEFKNFARGTKEVAKIIANLKECYSIIGGGDS
CCCCHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCC
IAAIEAEGLSNSFSHISTGGGASISFIENGDLIGLGPIQEKD
EEEEEECCCCCCHHHCCCCCCCEEEEEECCCEEEECCCCCCC
>Mature Secondary Structure
MQPTYKNKKFIDDIDFLNKTVILRVDFNVPIKNGEISSIKRIIASLKTIKKIVNNGGKLV
CCCCCCCCCHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEE
ILSHLGRIKSKEDLPKKSLRIVAEKLAEILGQEIKFIPENRGPKVENAISQLEKGEILVL
EEEHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHCCCCEEEE
ENTRFQDLNNKAESKNDAELGRYASLGDVFINDAFGTLHRAHGSNVGIATYIKESAIGYL
ECCCCHHCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
VKEELDALSKLIFSPQRPFYAIIGGAKISDKIGIISTLLEKADKVLIGGGMAYTFKKALG
HHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCEEEECCCHHHHHHHHHC
YKIGLSIYEDDKLELAASLVKKYPDKLILALDAALAPEFADLEPLYNQENPLEIPDHLEG
CEECCEEECCCHHHHHHHHHHHCCHHEEEHHHHHHCCCCCCCCHHCCCCCCCCCCCCCCC
MDIGPLTIELFKDHLKDGKTILNGTLGVAEFKNFARGTKEVAKIIANLKECYSIIGGGDS
CCCCHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCC
IAAIEAEGLSNSFSHISTGGGASISFIENGDLIGLGPIQEKD
EEEEEECCCCCCHHHCCCCCCCEEEEEECCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA