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The map label for this gene is pcrA

Identifier: 54020194

GI number: 54020194

Start: 495448

End: 497757

Strand: Direct

Name: pcrA

Synonym: mhp418

Alternate gene names: 54020194

Gene position: 495448-497757 (Clockwise)

Preceding gene: 54020193

Following gene: 54020195

Centisome position: 55.5

GC content: 31.39

Gene sequence:

>2310_bases
ATGCAATTCCGGCGAATTTTATCCCGGTTGATTGGAAAGGTAACTAAAATGTCATCGCAAAATATTTTATTACAACTTAA
TGAAAAACAAAAAATTGCTGTTATCAGTAACTCTAGCCATCTAAGAATTGTTGCTGGTGCTGGCACTGGTAAAACTAGTG
TGCTAACCAAAAAAATCGCTTATATTATCAACGAATCTCTTGCATATCCAAGTCGAATTTTAGCACTTACTTTTACAAAT
AAAGCCGCAGAAGAAATGCGTAATCGGGTAGAAAAATTAGTTGGGGAAAAATCAAAGGATATTCAAATTCTTACTTTTCA
TTCACTTTGTAATTTAATTCTCCGACTTGAAGCAAAAAATATTGTTGAACTCTCTGAGATTCCGCTTAGCGACTACCGTT
TTAACATTATTGATGAACAAGACCAGAAAAAAATTGTTGAAAAACTTTTAGGAAAATATCTAAAAGCGAATGATGACCGC
GATGAGACAAAAATTAGTGCTTTTGATGCAATTAAATTCATTTCTAATGCCAAAAATTGAGAAAAATCACCTGAGGATTT
GCTAAAAAGTATAAATAATGAACTTGATCAAATCAAAGTTCAAGTTTATCAAAAATATCTTGAAAAAACAAAAGAAAATA
ACATTATTGACTTTGATGATCTTTTACTTTATACTAAAATTGCCTTTGAAAAAAGTCCAGAAATTAGGTCTAGATGGCAA
AGAAAATTTGATTTTGTCCTTGTAGATGAATTCCAGGATACTTCCTTGGTTCAGTATTTAATTTTAAAAATTTTTATCAA
AGATAAAACACGTTTAGTTGTTGTCGGCGATCCTGATCAGACAATTTATTCTTGAAGAGGAGCCGATCCGTCTTTGATCT
TAAATTTAGAGCAAGATTTTCCCGATCTCCAGACGGTTATTCTTGATAAAAACTACCGATCAACGCAAAATATTCTCGAT
GCGGCTAATAATTTAATTGAAAATAACCAAAATCGGATCAAAAAGAAATTAGTTGCTAGTTCAAAGGAAAAAATCGAAAT
TCACTTTGAGGATTTAGCCAATGAAAGGGGAGCTGAAGTTGAATGAATTTACCGAAAAATTCAGGAATTAATCACAAAAA
ATAAGGTAACTTATCGCGATATCGCCATTTTAGCACGCTCAAATTTTTACTTTAAACAAATAGAAACTAAATTTGATGGC
CATAATATTCCGTATTTTCGGCACGGAGCTTCCCCGCTTGTAACCAAAAAAGAAATCCGTGAAGCGCTTTATTTTCTAAA
AGTAATTGATAATTCTGATCCTTATGCTTTTGAACAGATTATCAATGTCCCGCCAAAAAAAATTGGAATAGCAACAATTA
ACAAACTAAATGAATTAGCAGCCCAATTTGGTCTTAATTTACACGATTTTTTATTTAAATATTATTCTGGTAAAATAAAT
TTTAAAGGAAAACAAGGAAAAATTCCCTTAGGCCATGATAATCAGCTTAAAATTAAGGTACTTTTAGAAAGAATTAGCGC
CGCAAAACGGTTTAAAAGTCAAATTGAAGAAAAAAAACCTTGTATTTTTCAGTTTTACTCGCAAGTTCTTGACTCTTTTT
TGAAAAAAATTGATTATTTTGATGCAATTAAAGATGCAAAACAAGCCTCTGATACTCGCAATCTTTTAGAAAAATACTAT
AAAATAATGGATAATTGACAAAAAGCCAATCCTGAAAAAAAATTATCCGACTATCTTGATTATGCAGCTATTTCTCATTT
TGAACAAACTGAATCACAAAATAAAATTAATTTATTAACAGTACATTCCTCAAAAGGTCTTGAATTTGAATATGTTTTTC
TTGTGGGAATGAATCAAGGGGTTTTTCCGGCCCAAAAAGTACTCGATCAGAACTCGACAAGCGAATATGAAGAGGAAAGA
AGACTCGCTTTTGTAGCAGTTACGCGCGCAAAACGAGTTTTATATATAACAAATGGTTATCGCGCGCCTTCTTTTGCCCG
CGGTTTGCCGCAAAAATTTAAAACTAATCAAAATATTGTCTCTGCTTTTGTTAAAGAGATGAAAATTAAAGCTGAACGGT
TTGCCCAGTTTCGTAAACTTGATGAAACAGGAAAACTAAATTATACCAAAGATAAATCAGAAATAGTGGAATTTGTTGCT
GGTGACCAAATTAATCACTTAAAATTTGGTCGTGGCATCGTTTTAGAGGTCAGAATTGACTCAATTTTAGTTAAATTTTT
TGAAATTCCTGGCGATAAAGGAATTAAAACACTAATAAAAACACATAAATCAATCGATAAAATAACCTAA

Upstream 100 bases:

>100_bases
AATTTATCAATTTTTAGTAGGCATAAAACAACAATTATTGACCGTTATTCAAATACCGAAATTCATCTTAAAAAATCAGG
TCTAGCCAATAATTTTTTAA

Downstream 100 bases:

>100_bases
AATGCTATTAAATTGACAATCTTATTTACTCGTTGCCCTGATTATTTTAATCTCGCTTTCTTTTCTTCTTTTATTAATTT
TTAGCTTAGTTTTTATTTAT

Product: ATP-dependent helicase PcrA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 769; Mature: 769

Protein sequence:

>769_residues
MQFRRILSRLIGKVTKMSSQNILLQLNEKQKIAVISNSSHLRIVAGAGTGKTSVLTKKIAYIINESLAYPSRILALTFTN
KAAEEMRNRVEKLVGEKSKDIQILTFHSLCNLILRLEAKNIVELSEIPLSDYRFNIIDEQDQKKIVEKLLGKYLKANDDR
DETKISAFDAIKFISNAKNWEKSPEDLLKSINNELDQIKVQVYQKYLEKTKENNIIDFDDLLLYTKIAFEKSPEIRSRWQ
RKFDFVLVDEFQDTSLVQYLILKIFIKDKTRLVVVGDPDQTIYSWRGADPSLILNLEQDFPDLQTVILDKNYRSTQNILD
AANNLIENNQNRIKKKLVASSKEKIEIHFEDLANERGAEVEWIYRKIQELITKNKVTYRDIAILARSNFYFKQIETKFDG
HNIPYFRHGASPLVTKKEIREALYFLKVIDNSDPYAFEQIINVPPKKIGIATINKLNELAAQFGLNLHDFLFKYYSGKIN
FKGKQGKIPLGHDNQLKIKVLLERISAAKRFKSQIEEKKPCIFQFYSQVLDSFLKKIDYFDAIKDAKQASDTRNLLEKYY
KIMDNWQKANPEKKLSDYLDYAAISHFEQTESQNKINLLTVHSSKGLEFEYVFLVGMNQGVFPAQKVLDQNSTSEYEEER
RLAFVAVTRAKRVLYITNGYRAPSFARGLPQKFKTNQNIVSAFVKEMKIKAERFAQFRKLDETGKLNYTKDKSEIVEFVA
GDQINHLKFGRGIVLEVRIDSILVKFFEIPGDKGIKTLIKTHKSIDKIT

Sequences:

>Translated_769_residues
MQFRRILSRLIGKVTKMSSQNILLQLNEKQKIAVISNSSHLRIVAGAGTGKTSVLTKKIAYIINESLAYPSRILALTFTN
KAAEEMRNRVEKLVGEKSKDIQILTFHSLCNLILRLEAKNIVELSEIPLSDYRFNIIDEQDQKKIVEKLLGKYLKANDDR
DETKISAFDAIKFISNAKN*EKSPEDLLKSINNELDQIKVQVYQKYLEKTKENNIIDFDDLLLYTKIAFEKSPEIRSRWQ
RKFDFVLVDEFQDTSLVQYLILKIFIKDKTRLVVVGDPDQTIYS*RGADPSLILNLEQDFPDLQTVILDKNYRSTQNILD
AANNLIENNQNRIKKKLVASSKEKIEIHFEDLANERGAEVE*IYRKIQELITKNKVTYRDIAILARSNFYFKQIETKFDG
HNIPYFRHGASPLVTKKEIREALYFLKVIDNSDPYAFEQIINVPPKKIGIATINKLNELAAQFGLNLHDFLFKYYSGKIN
FKGKQGKIPLGHDNQLKIKVLLERISAAKRFKSQIEEKKPCIFQFYSQVLDSFLKKIDYFDAIKDAKQASDTRNLLEKYY
KIMDN*QKANPEKKLSDYLDYAAISHFEQTESQNKINLLTVHSSKGLEFEYVFLVGMNQGVFPAQKVLDQNSTSEYEEER
RLAFVAVTRAKRVLYITNGYRAPSFARGLPQKFKTNQNIVSAFVKEMKIKAERFAQFRKLDETGKLNYTKDKSEIVEFVA
GDQINHLKFGRGIVLEVRIDSILVKFFEIPGDKGIKTLIKTHKSIDKIT
>Mature_769_residues
MQFRRILSRLIGKVTKMSSQNILLQLNEKQKIAVISNSSHLRIVAGAGTGKTSVLTKKIAYIINESLAYPSRILALTFTN
KAAEEMRNRVEKLVGEKSKDIQILTFHSLCNLILRLEAKNIVELSEIPLSDYRFNIIDEQDQKKIVEKLLGKYLKANDDR
DETKISAFDAIKFISNAKN*EKSPEDLLKSINNELDQIKVQVYQKYLEKTKENNIIDFDDLLLYTKIAFEKSPEIRSRWQ
RKFDFVLVDEFQDTSLVQYLILKIFIKDKTRLVVVGDPDQTIYS*RGADPSLILNLEQDFPDLQTVILDKNYRSTQNILD
AANNLIENNQNRIKKKLVASSKEKIEIHFEDLANERGAEVE*IYRKIQELITKNKVTYRDIAILARSNFYFKQIETKFDG
HNIPYFRHGASPLVTKKEIREALYFLKVIDNSDPYAFEQIINVPPKKIGIATINKLNELAAQFGLNLHDFLFKYYSGKIN
FKGKQGKIPLGHDNQLKIKVLLERISAAKRFKSQIEEKKPCIFQFYSQVLDSFLKKIDYFDAIKDAKQASDTRNLLEKYY
KIMDN*QKANPEKKLSDYLDYAAISHFEQTESQNKINLLTVHSSKGLEFEYVFLVGMNQGVFPAQKVLDQNSTSEYEEER
RLAFVAVTRAKRVLYITNGYRAPSFARGLPQKFKTNQNIVSAFVKEMKIKAERFAQFRKLDETGKLNYTKDKSEIVEFVA
GDQINHLKFGRGIVLEVRIDSILVKFFEIPGDKGIKTLIKTHKSIDKIT

Specific function: Essential helicase [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=777, Percent_Identity=30.3732303732304, Blast_Score=310, Evalue=2e-85,
Organism=Escherichia coli, GI48994965, Length=655, Percent_Identity=32.824427480916, Blast_Score=289, Evalue=4e-79,
Organism=Escherichia coli, GI1787196, Length=339, Percent_Identity=27.4336283185841, Blast_Score=111, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6322369, Length=757, Percent_Identity=27.7410832232497, Blast_Score=163, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6324477, Length=683, Percent_Identity=24.597364568082, Blast_Score=113, Evalue=1e-25,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 88343; Mature: 88343

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFRRILSRLIGKVTKMSSQNILLQLNEKQKIAVISNSSHLRIVAGAGTGKTSVLTKKIA
CCHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCEEEEEEECCCCCHHHHHHHHH
YIINESLAYPSRILALTFTNKAAEEMRNRVEKLVGEKSKDIQILTFHSLCNLILRLEAKN
HHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHCCCC
IVELSEIPLSDYRFNIIDEQDQKKIVEKLLGKYLKANDDRDETKISAFDAIKFISNAKNE
CHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCC
KSPEDLLKSINNELDQIKVQVYQKYLEKTKENNIIDFDDLLLYTKIAFEKSPEIRSRWQR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHH
KFDFVLVDEFQDTSLVQYLILKIFIKDKTRLVVVGDPDQTIYSRGADPSLILNLEQDFPD
HCCEEEEECCCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHCCCCCEEEEEECCCCCC
LQTVILDKNYRSTQNILDAANNLIENNQNRIKKKLVASSKEKIEIHFEDLANERGAEVEI
HHEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEHHHHCCCCCCCHHH
YRKIQELITKNKVTYRDIAILARSNFYFKQIETKFDGHNIPYFRHGASPLVTKKEIREAL
HHHHHHHHHCCCCCHHHHEEHEECCCEEHHHHHCCCCCCCCCEECCCCCCCCHHHHHHHH
YFLKVIDNSDPYAFEQIINVPPKKIGIATINKLNELAAQFGLNLHDFLFKYYSGKINFKG
HHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCEEEECC
KQGKIPLGHDNQLKIKVLLERISAAKRFKSQIEEKKPCIFQFYSQVLDSFLKKIDYFDAI
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
KDAKQASDTRNLLEKYYKIMDNQKANPEKKLSDYLDYAAISHFEQTESQNKINLLTVHSS
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECC
KGLEFEYVFLVGMNQGVFPAQKVLDQNSTSEYEEERRLAFVAVTRAKRVLYITNGYRAPS
CCCEEEEEEEEECCCCCCHHHHHHCCCCCHHHHHHHHEEEEEEECCCEEEEEECCCCCCH
FARGLPQKFKTNQNIVSAFVKEMKIKAERFAQFRKLDETGKLNYTKDKSEIVEFVAGDQI
HHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCC
NHLKFGRGIVLEVRIDSILVKFFEIPGDKGIKTLIKTHKSIDKIT
CCEECCCCEEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQFRRILSRLIGKVTKMSSQNILLQLNEKQKIAVISNSSHLRIVAGAGTGKTSVLTKKIA
CCHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCEEEEEEECCCCCHHHHHHHHH
YIINESLAYPSRILALTFTNKAAEEMRNRVEKLVGEKSKDIQILTFHSLCNLILRLEAKN
HHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHCCCC
IVELSEIPLSDYRFNIIDEQDQKKIVEKLLGKYLKANDDRDETKISAFDAIKFISNAKNE
CHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCC
KSPEDLLKSINNELDQIKVQVYQKYLEKTKENNIIDFDDLLLYTKIAFEKSPEIRSRWQR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHH
KFDFVLVDEFQDTSLVQYLILKIFIKDKTRLVVVGDPDQTIYSRGADPSLILNLEQDFPD
HCCEEEEECCCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHCCCCCEEEEEECCCCCC
LQTVILDKNYRSTQNILDAANNLIENNQNRIKKKLVASSKEKIEIHFEDLANERGAEVEI
HHEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEHHHHCCCCCCCHHH
YRKIQELITKNKVTYRDIAILARSNFYFKQIETKFDGHNIPYFRHGASPLVTKKEIREAL
HHHHHHHHHCCCCCHHHHEEHEECCCEEHHHHHCCCCCCCCCEECCCCCCCCHHHHHHHH
YFLKVIDNSDPYAFEQIINVPPKKIGIATINKLNELAAQFGLNLHDFLFKYYSGKINFKG
HHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCEEEECC
KQGKIPLGHDNQLKIKVLLERISAAKRFKSQIEEKKPCIFQFYSQVLDSFLKKIDYFDAI
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
KDAKQASDTRNLLEKYYKIMDNQKANPEKKLSDYLDYAAISHFEQTESQNKINLLTVHSS
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECC
KGLEFEYVFLVGMNQGVFPAQKVLDQNSTSEYEEERRLAFVAVTRAKRVLYITNGYRAPS
CCCEEEEEEEEECCCCCCHHHHHHCCCCCHHHHHHHHEEEEEEECCCEEEEEECCCCCCH
FARGLPQKFKTNQNIVSAFVKEMKIKAERFAQFRKLDETGKLNYTKDKSEIVEFVAGDQI
HHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCC
NHLKFGRGIVLEVRIDSILVKFFEIPGDKGIKTLIKTHKSIDKIT
CCEECCCCEEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA