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The map label for this gene is pfkA

Identifier: 54020116

GI number: 54020116

Start: 295045

End: 296013

Strand: Direct

Name: pfkA

Synonym: mhp269

Alternate gene names: 54020116

Gene position: 295045-296013 (Clockwise)

Preceding gene: 54020115

Following gene: 54020117

Centisome position: 33.05

GC content: 34.16

Gene sequence:

>969_bases
ATGTCAAAAAAAATCGGTATATTAACTTCAGGCGGTGATGCGCCTGGGATGAATTCTGCAATAAGTTTTTTGGCAAAATC
AGCACTTAGTTTAGGTTTTGAGCCATATTTAATTTTTGATGGCTATTCTGGAATAATCGCAAGAAAAATATTGCCAGCAA
AAAATTTTCCTTATAATGGAATTTCTAGTTTTGGCGGAACTGCCATAGGATCAAGTCGCTTTCCTGAATTTAAAAAAGAG
GAAGTTCAAAATAAAGCCGTTGAAATTTTAAGTGAAATTGGGATTAGTTCGCTTGTGGTAGTTGGTGGCGATGGGACCTA
TAATGGCGGTTATAAATTACATTTAAAAGGAATAAAAGTAATCGCTTTACCAGGAACCATTGATAATGATATCCAGTTTA
CTGATTATACAATTGGATTTGATACTGCTTTGAATACGATAGTTGAAACTATTGATAAATTAAGGGATACGGCGAATTCT
CATCGTCGTTGTTTTGTAGTTGAAGTTATGGGGAGACATTGTCAAGATTTAGCTTTATATTCGGCAATAGCAACTGGAAG
CGAAATTTTAATTACTAATACAAATATTTTAACCCCTGAAGAGGTAAGTCAAAGAGTTTTAGAACAGTTTGCAAAAGGCA
AACCAAGCGTGATAGTAACAATTACAGAGAATATTTTACCAAACCTAAAAGAGTTTGCGGCTAAAATTGAGGAGTTAACA
AAAATTTCCACTCGCTCTTTAGAAGTTGGACATACCCAGCGCGGCGGCAGGCCTTCAGCTTTTGACCGAATTTTAGCAGC
CAAGATGGCAATGAAAGCTATGGAATTAATTAATCAAGATAAATCAGGTCTTGCAATTAGTTATTTAGATGGTAAAATCC
AAACTTTTGATATTGCGAAAGTAGTATCAAAACCTGTTAGAAAAACAAACGACTTAGTGCTAGAAATTAATAAAATCAAT
CAAAATTAA

Upstream 100 bases:

>100_bases
ATCATTGGGTCAGAGGTTCAAATCCTCTAGGGCGTACCATTTCAAATTTTTTGTCCTATTTTATTTATATATCTAAAAAG
GGAATTCTATTTAAGGTAAA

Downstream 100 bases:

>100_bases
AGGATTAAAATGCGAAAAATAGTAAAATTAAAAATGGCCAAAAGACGTGAACTAAGAAGACTTAAAACTTCAAAAGCGGC
AAAAAAAGCAAATGCGAAGT

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MSKKIGILTSGGDAPGMNSAISFLAKSALSLGFEPYLIFDGYSGIIARKILPAKNFPYNGISSFGGTAIGSSRFPEFKKE
EVQNKAVEILSEIGISSLVVVGGDGTYNGGYKLHLKGIKVIALPGTIDNDIQFTDYTIGFDTALNTIVETIDKLRDTANS
HRRCFVVEVMGRHCQDLALYSAIATGSEILITNTNILTPEEVSQRVLEQFAKGKPSVIVTITENILPNLKEFAAKIEELT
KISTRSLEVGHTQRGGRPSAFDRILAAKMAMKAMELINQDKSGLAISYLDGKIQTFDIAKVVSKPVRKTNDLVLEINKIN
QN

Sequences:

>Translated_322_residues
MSKKIGILTSGGDAPGMNSAISFLAKSALSLGFEPYLIFDGYSGIIARKILPAKNFPYNGISSFGGTAIGSSRFPEFKKE
EVQNKAVEILSEIGISSLVVVGGDGTYNGGYKLHLKGIKVIALPGTIDNDIQFTDYTIGFDTALNTIVETIDKLRDTANS
HRRCFVVEVMGRHCQDLALYSAIATGSEILITNTNILTPEEVSQRVLEQFAKGKPSVIVTITENILPNLKEFAAKIEELT
KISTRSLEVGHTQRGGRPSAFDRILAAKMAMKAMELINQDKSGLAISYLDGKIQTFDIAKVVSKPVRKTNDLVLEINKIN
QN
>Mature_321_residues
SKKIGILTSGGDAPGMNSAISFLAKSALSLGFEPYLIFDGYSGIIARKILPAKNFPYNGISSFGGTAIGSSRFPEFKKEE
VQNKAVEILSEIGISSLVVVGGDGTYNGGYKLHLKGIKVIALPGTIDNDIQFTDYTIGFDTALNTIVETIDKLRDTANSH
RRCFVVEVMGRHCQDLALYSAIATGSEILITNTNILTPEEVSQRVLEQFAKGKPSVIVTITENILPNLKEFAAKIEELTK
ISTRSLEVGHTQRGGRPSAFDRILAAKMAMKAMELINQDKSGLAISYLDGKIQTFDIAKVVSKPVRKTNDLVLEINKINQ
N

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family

Homologues:

Organism=Homo sapiens, GI11321601, Length=308, Percent_Identity=37.6623376623377, Blast_Score=178, Evalue=5e-45,
Organism=Homo sapiens, GI48762920, Length=308, Percent_Identity=37.3376623376623, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI266453768, Length=308, Percent_Identity=33.4415584415584, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI266453748, Length=308, Percent_Identity=33.4415584415584, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI4505749, Length=308, Percent_Identity=33.4415584415584, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI266453619, Length=308, Percent_Identity=33.4415584415584, Blast_Score=159, Evalue=3e-39,
Organism=Escherichia coli, GI1790350, Length=307, Percent_Identity=45.2768729641694, Blast_Score=261, Evalue=3e-71,
Organism=Caenorhabditis elegans, GI25147584, Length=308, Percent_Identity=34.7402597402597, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17558788, Length=323, Percent_Identity=33.1269349845201, Blast_Score=168, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI72003601, Length=291, Percent_Identity=27.8350515463918, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6323861, Length=312, Percent_Identity=33.974358974359, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6321679, Length=310, Percent_Identity=32.9032258064516, Blast_Score=155, Evalue=7e-39,
Organism=Drosophila melanogaster, GI28573326, Length=305, Percent_Identity=35.7377049180328, Blast_Score=173, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24652337, Length=305, Percent_Identity=35.0819672131148, Blast_Score=170, Evalue=1e-42,
Organism=Drosophila melanogaster, GI17647809, Length=305, Percent_Identity=35.0819672131148, Blast_Score=169, Evalue=2e-42,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): K6PF_MYCH2 (Q601D3)

Other databases:

- EMBL:   AE017332
- RefSeq:   YP_115782.1
- HSSP:   P0A796
- ProteinModelPortal:   Q601D3
- SMR:   Q601D3
- STRING:   Q601D3
- GeneID:   3105354
- GenomeReviews:   AE017332_GR
- KEGG:   mhy:mhp269
- eggNOG:   COG0205
- HOGENOM:   HBG405044
- OMA:   RDTANSH
- PhylomeDB:   Q601D3
- ProtClustDB:   CLSK741310
- BioCyc:   MHYO295358:MHP269-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00339
- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023
- PIRSF:   PIRSF000532
- PRINTS:   PR00476
- TIGRFAMs:   TIGR02482

Pfam domain/function: PF00365 PFK; SSF53784 Ppfruckinase

EC number: =2.7.1.11

Molecular weight: Translated: 34962; Mature: 34831

Theoretical pI: Translated: 8.94; Mature: 8.94

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: ACT_SITE 128-128 BINDING 163-163 BINDING 245-245 BINDING 251-251 BINDING 254-254

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKIGILTSGGDAPGMNSAISFLAKSALSLGFEPYLIFDGYSGIIARKILPAKNFPYNG
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHCCCCCCCCCC
ISSFGGTAIGSSRFPEFKKEEVQNKAVEILSEIGISSLVVVGGDGTYNGGYKLHLKGIKV
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEECEEE
IALPGTIDNDIQFTDYTIGFDTALNTIVETIDKLRDTANSHRRCFVVEVMGRHCQDLALY
EEECCCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHH
SAIATGSEILITNTNILTPEEVSQRVLEQFAKGKPSVIVTITENILPNLKEFAAKIEELT
HHHHCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEECHHHCCCHHHHHHHHHHHH
KISTRSLEVGHTQRGGRPSAFDRILAAKMAMKAMELINQDKSGLAISYLDGKIQTFDIAK
HHHHCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEHHHHH
VVSKPVRKTNDLVLEINKINQN
HHHHHHCCCCCEEEEEEECCCC
>Mature Secondary Structure 
SKKIGILTSGGDAPGMNSAISFLAKSALSLGFEPYLIFDGYSGIIARKILPAKNFPYNG
CCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHCCCCCCCCCC
ISSFGGTAIGSSRFPEFKKEEVQNKAVEILSEIGISSLVVVGGDGTYNGGYKLHLKGIKV
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEECEEE
IALPGTIDNDIQFTDYTIGFDTALNTIVETIDKLRDTANSHRRCFVVEVMGRHCQDLALY
EEECCCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHH
SAIATGSEILITNTNILTPEEVSQRVLEQFAKGKPSVIVTITENILPNLKEFAAKIEELT
HHHHCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEECHHHCCCHHHHHHHHHHHH
KISTRSLEVGHTQRGGRPSAFDRILAAKMAMKAMELINQDKSGLAISYLDGKIQTFDIAK
HHHHCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEHHHHH
VVSKPVRKTNDLVLEINKINQN
HHHHHHCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA