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The map label for this gene is celM

Identifier: 54020109

GI number: 54020109

Start: 278447

End: 279517

Strand: Direct

Name: celM

Synonym: mhp252

Alternate gene names: 54020109

Gene position: 278447-279517 (Clockwise)

Preceding gene: 54020108

Following gene: 54020110

Centisome position: 31.19

GC content: 35.29

Gene sequence:

>1071_bases
ATGTCAATATTAGAAAAAATGAAAAAATATTGCGACATTGATGGTATGTCGCGTTATGAGGATGAAGTTGTTGCTGAATT
AAAAAGATCAACAGAAAAACTTGATCTTGTCTATTCTCGTGATGGTTTTGGTTCCTTAATTATGCAGAAAAAAAATCCAA
ATTCTGGACCTAAAGTAGTCATTGCCGCGCATATGGATGAAGTTGGTTTTATCGTTTTAGATATTACTGAAAAAGGTTTT
ATCAAGGTTAAATCAATTGGCGGAATTTGAGGAAATGCGGTAATAGGGGCAAGATTTAAGTTAATTAGTTCATCAGGTCA
GGAGTTTTTTGGGGTTGCTGGGCATACTTCAATTCATATTATGGAGAGATCTAAAATTGAAAAAGCTTTACTAGTAAAGG
ATCTTTTTTTTGATTTTGGTTTTAGATCTAAAAAGGAAGCTCAGGATTTAGGTGTTGAAATTGGTAATCGAATTTATTTT
AATAATCCTAGTTTTTTGATGCAAAATCAAGATTTTTTTGCATCAAAAGCAATTGACAACCGCGCAGGCGTGGTTGTCAT
TGATGAATTAGCACATCGATTATATAATAAGGAATTAAAATCTAATCCGATTTTAGTAGGAACTGTCCAAGAAGAAGTTG
GTTCCCGTGGGGCAAAAATGGTTGCAAAAATGATTAATGCTGATGTTGCTTTTGCAATCGATACAGGAGCATCCCATGAT
ACTGAAGGCGCAATTCCTGGTGTTCAAAAACTAGGTGCAGGAGTTGCAATTGATATTGCCGATGGAGGCGCTTTAATGGA
CCCACGGTTAGTTGAAATTCTTTTTAGGATTGCCAAGGAAAGAAACATCCCGATTTATCGTTATGTTTCGCAAGGTGGGG
GAACTGATGCTGAAAAATTACAATATTCAGGTTATGGGACCCCAACAGTAAGTATTTCTGTTCCCCAGCGTTACCTCCAT
TCAACTTATGGTCTAATTAGTTTATCAGATATTAAAGCCGCAGTTGATCTAATCGAAGCTTTCATTCTTGAATATGGCCA
AAAAGAACATCAAGAGTTAATATATAAATAA

Upstream 100 bases:

>100_bases
GATTTTTTAAAAGAAAAAAAACGATTAGAAATTAATTGGGATTTATAAAAAAAAAGTGTATAATTTTCTAAATTATATCA
TAAAAGAAAGGATTTTTTAA

Downstream 100 bases:

>100_bases
CAGCTGGAAAATAGTAATTTTTCTTAACTAATAAATAAAGTAAATTGGCTGTTTTCCAACTTTTTCAGTTTCTGCTTTAG
CCATTTTTGAATCATCTCAT

Product: endo-1,4-beta-glucanase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MSILEKMKKYCDIDGMSRYEDEVVAELKRSTEKLDLVYSRDGFGSLIMQKKNPNSGPKVVIAAHMDEVGFIVLDITEKGF
IKVKSIGGIWGNAVIGARFKLISSSGQEFFGVAGHTSIHIMERSKIEKALLVKDLFFDFGFRSKKEAQDLGVEIGNRIYF
NNPSFLMQNQDFFASKAIDNRAGVVVIDELAHRLYNKELKSNPILVGTVQEEVGSRGAKMVAKMINADVAFAIDTGASHD
TEGAIPGVQKLGAGVAIDIADGGALMDPRLVEILFRIAKERNIPIYRYVSQGGGTDAEKLQYSGYGTPTVSISVPQRYLH
STYGLISLSDIKAAVDLIEAFILEYGQKEHQELIYK

Sequences:

>Translated_356_residues
MSILEKMKKYCDIDGMSRYEDEVVAELKRSTEKLDLVYSRDGFGSLIMQKKNPNSGPKVVIAAHMDEVGFIVLDITEKGF
IKVKSIGGI*GNAVIGARFKLISSSGQEFFGVAGHTSIHIMERSKIEKALLVKDLFFDFGFRSKKEAQDLGVEIGNRIYF
NNPSFLMQNQDFFASKAIDNRAGVVVIDELAHRLYNKELKSNPILVGTVQEEVGSRGAKMVAKMINADVAFAIDTGASHD
TEGAIPGVQKLGAGVAIDIADGGALMDPRLVEILFRIAKERNIPIYRYVSQGGGTDAEKLQYSGYGTPTVSISVPQRYLH
STYGLISLSDIKAAVDLIEAFILEYGQKEHQELIYK
>Mature_355_residues
SILEKMKKYCDIDGMSRYEDEVVAELKRSTEKLDLVYSRDGFGSLIMQKKNPNSGPKVVIAAHMDEVGFIVLDITEKGFI
KVKSIGGI*GNAVIGARFKLISSSGQEFFGVAGHTSIHIMERSKIEKALLVKDLFFDFGFRSKKEAQDLGVEIGNRIYFN
NPSFLMQNQDFFASKAIDNRAGVVVIDELAHRLYNKELKSNPILVGTVQEEVGSRGAKMVAKMINADVAFAIDTGASHDT
EGAIPGVQKLGAGVAIDIADGGALMDPRLVEILFRIAKERNIPIYRYVSQGGGTDAEKLQYSGYGTPTVSISVPQRYLHS
TYGLISLSDIKAAVDLIEAFILEYGQKEHQELIYK

Specific function: Putative aminopeptidase [H]

COG id: COG1363

COG function: function code G; Cellulase M and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M42 family [H]

Homologues:

Organism=Escherichia coli, GI1790332, Length=347, Percent_Identity=33.1412103746398, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI87082417, Length=334, Percent_Identity=29.0419161676647, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1788727, Length=340, Percent_Identity=27.6470588235294, Blast_Score=130, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008007 [H]

Pfam domain/function: PF05343 Peptidase_M42 [H]

EC number: NA

Molecular weight: Translated: 39039; Mature: 38908

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSILEKMKKYCDIDGMSRYEDEVVAELKRSTEKLDLVYSRDGFGSLIMQKKNPNSGPKVV
CCHHHHHHHHCCCCCCHHHHHHHHHHHHHCHHHEEEEEECCCCCEEEEECCCCCCCCEEE
IAAHMDEVGFIVLDITEKGFIKVKSIGGIGNAVIGARFKLISSSGQEFFGVAGHTSIHIM
EEEECCCCCEEEEEECCCCEEEEEECCCCCCHHHHHEEEEECCCCCCEEEECCCCEEEEE
ERSKIEKALLVKDLFFDFGFRSKKEAQDLGVEIGNRIYFNNPSFLMQNQDFFASKAIDNR
EHHHHHHHHHHHHHHHHCCCCCCHHHHHHCHHHCCEEEECCCCEEEECCCHHHHHCCCCC
AGVVVIDELAHRLYNKELKSNPILVGTVQEEVGSRGAKMVAKMINADVAFAIDTGASHDT
CCEEEHHHHHHHHHHHHHCCCCEEEEECHHHHCCHHHHHHHHHHCCCEEEEEECCCCCCC
EGAIPGVQKLGAGVAIDIADGGALMDPRLVEILFRIAKERNIPIYRYVSQGGGTDAEKLQ
CCCCCCHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHEE
YSGYGTPTVSISVPQRYLHSTYGLISLSDIKAAVDLIEAFILEYGQKEHQELIYK
ECCCCCCEEEEECCHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHCCHHHHHHCCC
>Mature Secondary Structure 
SILEKMKKYCDIDGMSRYEDEVVAELKRSTEKLDLVYSRDGFGSLIMQKKNPNSGPKVV
CHHHHHHHHCCCCCCHHHHHHHHHHHHHCHHHEEEEEECCCCCEEEEECCCCCCCCEEE
IAAHMDEVGFIVLDITEKGFIKVKSIGGIGNAVIGARFKLISSSGQEFFGVAGHTSIHIM
EEEECCCCCEEEEEECCCCEEEEEECCCCCCHHHHHEEEEECCCCCCEEEECCCCEEEEE
ERSKIEKALLVKDLFFDFGFRSKKEAQDLGVEIGNRIYFNNPSFLMQNQDFFASKAIDNR
EHHHHHHHHHHHHHHHHCCCCCCHHHHHHCHHHCCEEEECCCCEEEECCCHHHHHCCCCC
AGVVVIDELAHRLYNKELKSNPILVGTVQEEVGSRGAKMVAKMINADVAFAIDTGASHDT
CCEEEHHHHHHHHHHHHHCCCCEEEEECHHHHCCHHHHHHHHHHCCCEEEEEECCCCCCC
EGAIPGVQKLGAGVAIDIADGGALMDPRLVEILFRIAKERNIPIYRYVSQGGGTDAEKLQ
CCCCCCHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHEE
YSGYGTPTVSISVPQRYLHSTYGLISLSDIKAAVDLIEAFILEYGQKEHQELIYK
ECCCCCCEEEEECCHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]