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The map label for this gene is rpoA

Identifier: 54020095

GI number: 54020095

Start: 242811

End: 243809

Strand: Direct

Name: rpoA

Synonym: mhp213

Alternate gene names: 54020095

Gene position: 242811-243809 (Clockwise)

Preceding gene: 54020094

Following gene: 54020096

Centisome position: 27.2

GC content: 32.73

Gene sequence:

>999_bases
ATGAAAAAACACGCAAAAGTTTATTATTCTGAAAATCTTGTTGAACAAGTTAATGAATTTGAAACAAGCTTTGAAATAAA
ACCACTCGAACGCGGACTCGGTAATACCTTAGGAAATGCGCTTCGCCGAACAGTTTTATCGTCAATTCCTTCCTGTGCAG
TTTTTGGTGTTAAAATTGAGGGAATAAAACATGAATTTACCGTTCTTGATGATGTAATTGAAGATGTTGTCACAATTCTT
AATAATTTAAAAAGAGTACGTTTTTTCTATAATCCAAGTGTTTTTAGTCAAAATTCAATCCACGTGGCAAGTTTTTTAGG
TCAAAAAGCAGGGCAAATTTATGCCCGTGATATTGAATCTCACAGTGGTCTTAAAATTGTAAATCCGGATTTATATATTG
CTGATGTTTCCAAAGTAGGAGCTTTAAAATTTGAGCTTTTTATTACAAATGGTAAAGGATTTGTAGATTTTGAAACAAAC
AAAAAGTATGTAAATGAGGTAATTTCGCTGCTTGAGTCCCAAATTGAAGGCTCTGTTTTAGCCGTTGATAGTGATTTTTC
GCCTGTATTAAATGCAAATTATCAAGCTGTTGAAATTAACTCAGCAAGTCCAATAATTGAAGAAAAACTAAATTTTTCAA
TTAAAACTGATGGATCTATTTTTGCAAAAGATGCTCTTTCGCAGGGGGCAAAAATTCTTATTGCCCACCTTAATTTGCTT
GCCGATGTCGAAAACCTTAATAAATTCAGTGCTGATTTCTTTGGTGATCAAGAGATTAAAGAAGAACCTATCCGCCGTTT
TTCAAATAGTATTGATGCACTTGATCTTTCTGTGCGTTCACTTAATGCCCTTCGCCGCGCGCAGTATTATAAAATTTCAG
ATATTGAAAAACTTAGTCAAGATGATTTTGAGAATATTAAAAATCTTGGACGAAAATCGGTGCAAGAGATAATGGAAAAA
CTACAAAACTATAAAAACGAAAATAAAGGAGAAAATTAA

Upstream 100 bases:

>100_bases
ACTTAACATTTTATTAACAAAAGATGTAACTCCAGTTCCACATAATGGAACACGCCCGCCTCGAAAATGATTTAAACGCC
AGGAAAAAAGGTAAAAACTT

Downstream 100 bases:

>100_bases
AAATGGCAAATCCACATCAAATTTATCGGCATGATGCTGCCTGAGATCGCCAAGTTTTTCGTTCACTTGCAACCTCTTTA
ATTTTACATGGACATATTAA

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MKKHAKVYYSENLVEQVNEFETSFEIKPLERGLGNTLGNALRRTVLSSIPSCAVFGVKIEGIKHEFTVLDDVIEDVVTIL
NNLKRVRFFYNPSVFSQNSIHVASFLGQKAGQIYARDIESHSGLKIVNPDLYIADVSKVGALKFELFITNGKGFVDFETN
KKYVNEVISLLESQIEGSVLAVDSDFSPVLNANYQAVEINSASPIIEEKLNFSIKTDGSIFAKDALSQGAKILIAHLNLL
ADVENLNKFSADFFGDQEIKEEPIRRFSNSIDALDLSVRSLNALRRAQYYKISDIEKLSQDDFENIKNLGRKSVQEIMEK
LQNYKNENKGEN

Sequences:

>Translated_332_residues
MKKHAKVYYSENLVEQVNEFETSFEIKPLERGLGNTLGNALRRTVLSSIPSCAVFGVKIEGIKHEFTVLDDVIEDVVTIL
NNLKRVRFFYNPSVFSQNSIHVASFLGQKAGQIYARDIESHSGLKIVNPDLYIADVSKVGALKFELFITNGKGFVDFETN
KKYVNEVISLLESQIEGSVLAVDSDFSPVLNANYQAVEINSASPIIEEKLNFSIKTDGSIFAKDALSQGAKILIAHLNLL
ADVENLNKFSADFFGDQEIKEEPIRRFSNSIDALDLSVRSLNALRRAQYYKISDIEKLSQDDFENIKNLGRKSVQEIMEK
LQNYKNENKGEN
>Mature_332_residues
MKKHAKVYYSENLVEQVNEFETSFEIKPLERGLGNTLGNALRRTVLSSIPSCAVFGVKIEGIKHEFTVLDDVIEDVVTIL
NNLKRVRFFYNPSVFSQNSIHVASFLGQKAGQIYARDIESHSGLKIVNPDLYIADVSKVGALKFELFITNGKGFVDFETN
KKYVNEVISLLESQIEGSVLAVDSDFSPVLNANYQAVEINSASPIIEEKLNFSIKTDGSIFAKDALSQGAKILIAHLNLL
ADVENLNKFSADFFGDQEIKEEPIRRFSNSIDALDLSVRSLNALRRAQYYKISDIEKLSQDDFENIKNLGRKSVQEIMEK
LQNYKNENKGEN

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=317, Percent_Identity=31.2302839116719, Blast_Score=130, Evalue=1e-31,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_MYCH2 (Q601I9)

Other databases:

- EMBL:   AE017332
- RefSeq:   YP_115726.1
- ProteinModelPortal:   Q601I9
- STRING:   Q601I9
- GeneID:   3105345
- GenomeReviews:   AE017332_GR
- KEGG:   mhy:mhp213
- eggNOG:   COG0202
- HOGENOM:   HBG430844
- OMA:   FGTTLGN
- ProtClustDB:   PRK05182
- BioCyc:   MHYO295358:MHP213-MONOMER
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 37331; Mature: 37331

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKHAKVYYSENLVEQVNEFETSFEIKPLERGLGNTLGNALRRTVLSSIPSCAVFGVKIE
CCCCCCEEHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEEEE
GIKHEFTVLDDVIEDVVTILNNLKRVRFFYNPSVFSQNSIHVASFLGQKAGQIYARDIES
CCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHHHHCHHHHHHHCC
HSGLKIVNPDLYIADVSKVGALKFELFITNGKGFVDFETNKKYVNEVISLLESQIEGSVL
CCCCEEECCCEEEEEHHHCCEEEEEEEEECCCEEEEECCCHHHHHHHHHHHHHHHCCCEE
AVDSDFSPVLNANYQAVEINSASPIIEEKLNFSIKTDGSIFAKDALSQGAKILIAHLNLL
EECCCCCHHHCCCEEEEEECCCCCHHHHHCCEEEECCCCCHHHHHHHCCHHHHHHHHHHH
ADVENLNKFSADFFGDQEIKEEPIRRFSNSIDALDLSVRSLNALRRAQYYKISDIEKLSQ
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCH
DDFENIKNLGRKSVQEIMEKLQNYKNENKGEN
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKKHAKVYYSENLVEQVNEFETSFEIKPLERGLGNTLGNALRRTVLSSIPSCAVFGVKIE
CCCCCCEEHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEEEE
GIKHEFTVLDDVIEDVVTILNNLKRVRFFYNPSVFSQNSIHVASFLGQKAGQIYARDIES
CCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHHHHCHHHHHHHCC
HSGLKIVNPDLYIADVSKVGALKFELFITNGKGFVDFETNKKYVNEVISLLESQIEGSVL
CCCCEEECCCEEEEEHHHCCEEEEEEEEECCCEEEEECCCHHHHHHHHHHHHHHHCCCEE
AVDSDFSPVLNANYQAVEINSASPIIEEKLNFSIKTDGSIFAKDALSQGAKILIAHLNLL
EECCCCCHHHCCCEEEEEECCCCCHHHHHCCEEEECCCCCHHHHHHHCCHHHHHHHHHHH
ADVENLNKFSADFFGDQEIKEEPIRRFSNSIDALDLSVRSLNALRRAQYYKISDIEKLSQ
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCH
DDFENIKNLGRKSVQEIMEKLQNYKNENKGEN
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA