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The map label for this gene is phoB [H]

Identifier: 53719597

GI number: 53719597

Start: 2367951

End: 2368679

Strand: Reverse

Name: phoB [H]

Synonym: BPSL1985

Alternate gene names: 53719597

Gene position: 2368679-2367951 (Counterclockwise)

Preceding gene: 53719598

Following gene: 53719596

Centisome position: 58.13

GC content: 63.65

Gene sequence:

>729_bases
ATGGATTTCGTTTTGCGGGGTGCTATGAGAATTGCTGTACTGGATGACGATCAGGCCCAGACGGACTTTGTCAGTCAGAC
GCTGACGGCCGCCGGCCATACGTGCTATGCGTTCAAGGAAGGCAAGGCGCTGAAGAAGCGGCTGCAGCGCGAAACCTTCG
ACTTGCTCGTGCTCGACTGGAACGTGCCGGACATGTCCGGCGAGGAAGTGCTCAAGTGGGTGCGGGCGAACCAGGTCGAG
CATCGCCTGCCGATCATCTTCATGACGAGCCGTGACGACGAGGCGGGCATCACGCAGATCCTGAACGCGGGCGCGGACGA
CTACGTCGTGAAGCCCGTGTCCGGCCCGATCCTGCGCGCGCGCATCGGCTCGCTGCTGCGCCGCGCGTATCCGGTGAACG
CGGAATCGTCGATCCGCGAGTTCGACAACTACAAGTTCGACGCGAACCTGAAGCAGGCGTACGTCGGCGACAAGCCGGTG
AGCCTCACGCAGAAGGAGTTCGAGCTCGCGTTGCTGCTGTTCCAGCATCTGGACCGGCCGCTGTCGCGCGCGCACATCCT
CGATCTCGTCTGGAAGCAGGCCACCGACATTCCGTCGCGCACGATGGACACGCATATCTCGATGCTGCGCACGAAGCTCG
GCCTGCGCCCGGAGAACGGCTACCGCCTCGCGCCGATCTACGGCTACGGCTATCGCCTCGAGCGCGTGATGCAGGGGGAC
GCGGAGTGA

Upstream 100 bases:

>100_bases
CCGGACAAACCTTCATCATTCAGCCGATAATGGCGAGTTACGACCAATCGGGGGGCTCTGCAGGCCTGCTCATTGCCGCT
CGGCAGTCGCGGGAGCCTGA

Downstream 100 bases:

>100_bases
GCGCGCGCGGGTTCGTGACGAAGTACGTGCTGACGGCATGCTGTGCCGCAGCGACGGCGCTCGCGGCGCAGCACGCCGCC
GCGCAGTCGGCTAAGAACCG

Product: putative two component system response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MDFVLRGAMRIAVLDDDQAQTDFVSQTLTAAGHTCYAFKEGKALKKRLQRETFDLLVLDWNVPDMSGEEVLKWVRANQVE
HRLPIIFMTSRDDEAGITQILNAGADDYVVKPVSGPILRARIGSLLRRAYPVNAESSIREFDNYKFDANLKQAYVGDKPV
SLTQKEFELALLLFQHLDRPLSRAHILDLVWKQATDIPSRTMDTHISMLRTKLGLRPENGYRLAPIYGYGYRLERVMQGD
AE

Sequences:

>Translated_242_residues
MDFVLRGAMRIAVLDDDQAQTDFVSQTLTAAGHTCYAFKEGKALKKRLQRETFDLLVLDWNVPDMSGEEVLKWVRANQVE
HRLPIIFMTSRDDEAGITQILNAGADDYVVKPVSGPILRARIGSLLRRAYPVNAESSIREFDNYKFDANLKQAYVGDKPV
SLTQKEFELALLLFQHLDRPLSRAHILDLVWKQATDIPSRTMDTHISMLRTKLGLRPENGYRLAPIYGYGYRLERVMQGD
AE
>Mature_242_residues
MDFVLRGAMRIAVLDDDQAQTDFVSQTLTAAGHTCYAFKEGKALKKRLQRETFDLLVLDWNVPDMSGEEVLKWVRANQVE
HRLPIIFMTSRDDEAGITQILNAGADDYVVKPVSGPILRARIGSLLRRAYPVNAESSIREFDNYKFDANLKQAYVGDKPV
SLTQKEFELALLLFQHLDRPLSRAHILDLVWKQATDIPSRTMDTHISMLRTKLGLRPENGYRLAPIYGYGYRLERVMQGD
AE

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790860, Length=228, Percent_Identity=32.4561403508772, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1786599, Length=227, Percent_Identity=29.9559471365639, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1789809, Length=228, Percent_Identity=29.3859649122807, Blast_Score=101, Evalue=4e-23,
Organism=Escherichia coli, GI1789402, Length=226, Percent_Identity=30.5309734513274, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87082012, Length=223, Percent_Identity=29.5964125560538, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1790552, Length=225, Percent_Identity=28.4444444444444, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1786784, Length=229, Percent_Identity=27.0742358078603, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1786911, Length=226, Percent_Identity=27.4336283185841, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1787229, Length=211, Percent_Identity=28.9099526066351, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788394, Length=232, Percent_Identity=28.448275862069, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI2367329, Length=228, Percent_Identity=29.8245614035088, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787375, Length=227, Percent_Identity=25.1101321585903, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1790863, Length=230, Percent_Identity=24.7826086956522, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788191, Length=121, Percent_Identity=27.2727272727273, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 27587; Mature: 27587

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFVLRGAMRIAVLDDDQAQTDFVSQTLTAAGHTCYAFKEGKALKKRLQRETFDLLVLDW
CCCEEECCEEEEEECCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHCEEEEEEEC
NVPDMSGEEVLKWVRANQVEHRLPIIFMTSRDDEAGITQILNAGADDYVVKPVSGPILRA
CCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCEEEECCCCHHHHH
RIGSLLRRAYPVNAESSIREFDNYKFDANLKQAYVGDKPVSLTQKEFELALLLFQHLDRP
HHHHHHHHHCCCCCHHHHHHHHCCEECCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHCCH
LSRAHILDLVWKQATDIPSRTMDTHISMLRTKLGLRPENGYRLAPIYGYGYRLERVMQGD
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHCCC
AE
CC
>Mature Secondary Structure
MDFVLRGAMRIAVLDDDQAQTDFVSQTLTAAGHTCYAFKEGKALKKRLQRETFDLLVLDW
CCCEEECCEEEEEECCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHCEEEEEEEC
NVPDMSGEEVLKWVRANQVEHRLPIIFMTSRDDEAGITQILNAGADDYVVKPVSGPILRA
CCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCEEEECCCCHHHHH
RIGSLLRRAYPVNAESSIREFDNYKFDANLKQAYVGDKPVSLTQKEFELALLLFQHLDRP
HHHHHHHHHCCCCCHHHHHHHHCCEECCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHCCH
LSRAHILDLVWKQATDIPSRTMDTHISMLRTKLGLRPENGYRLAPIYGYGYRLERVMQGD
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHCCC
AE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]