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The map label for this gene is tgt

Identifier: 52842932

GI number: 52842932

Start: 3078392

End: 3079558

Strand: Reverse

Name: tgt

Synonym: lpg2727

Alternate gene names: 52842932

Gene position: 3079558-3078392 (Counterclockwise)

Preceding gene: 52842933

Following gene: 52842931

Centisome position: 90.64

GC content: 40.62

Gene sequence:

>1167_bases
TTGCCGTTTCTGCGAATGAACACTTTTTCATGTGAAGTACTGTACCAGCATTCGGGTACCAAAGCTCGTGTTACACGTGT
GGTTACGCCTCATGGTGAATTTATAACTCCGGTATTTATGCCAGTTGGAACCCGTGCAGGGGTCAATAACATGATGCCGT
CAGAACTGTCTGCTGCGGGTACTCAAATTATTCTGGGCGGTAATACCTATCATATGTTGTGTGCGCCTGGCATGAAGGTG
ATAGAAAATGCAGGGGGGATGCATCGCTTTATGGGGTGGCATGGACCTATGCTAACAGACAGCGGTGGTTTTCAAGTTTT
TAGTTTATCGAAGAACAAGGAAATTTGTTCTATAGATGAATCTGGTGCTCATTTTAAATTGCCTGGCAGTAAGGGGATTA
TCCATATGACCCCGGAAATGTCTCTGGAGACCCAGAAAATAATTGGTGCCGATATTATAATGGCATTTGATCAGTGTACT
CCTGACAGCTGCTCCAAGGATGAAGTAAGTCAAATCATGTTGCGGACCCACAGATGGCTAAAACAATCGATTGACTACCA
TCAACAATATCCCAACTCTCGATACGGATATAAACAAGCTTTATTTGGCATTGTGCAAGGAGGAGTTTTTCAAGATTTAC
GTCGAGAAAGTGCTGATTTTGTTTCTGCAATGAATACGGATGGTATTGCAATAGGTGGAGAAACTGTAGGCTTTGATATG
GATACTACAGTGGAAGTCATTCGTTGGGTGTATGAGTATCTTCCGGGCAATAAGCCACGTTATACTATGGGAGTTGGAAT
GTCACCTCAAGATCTGTTGGATGTGGTGGCGGAAGGTATCGATATGTTTGATTGTGTTGCACCAACACGTAATGCCCGAC
ATGGTGCCTTATATTGTGGTAAATTGGTAAGAGAAGGGAATTGGCTAAAATTTCAAAGCGAACATGAAAATGGACGAATT
CAAATAAAGAAATCATGTTTTGCTGATGATTTGGCACCTGTTATGGAGAGTTGTTCTTGTTATACTTGTCTTAATTATTC
AAGATCCTATTTGCATCATTTATCAAAACAAAAAGCTAATTTATTTACTGCTTTGGCAAGCATCCATAATGTTCATGTTT
TGCATGATGTTTGTAATCACATGCGTCATTTGATAATAAATTCCTAA

Upstream 100 bases:

>100_bases
TTTATGGCGATAATGGGGCTTTTTCTGTTTTTTCGAACCCGAACGATCAATTAATTCCTGGCATTAAACGCGTGCTTTCG
TTATCATTCTTTGTTTTATC

Downstream 100 bases:

>100_bases
ATTATTTAATATTAAGAGGTGAAATAATGGTTTTAATTTCTACATCCATGGGTGATATTCATATCAAATTAGATACAGAA
AATACCCCATTAACTGCAGA

Product: queuine/archaeosine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 388; Mature: 387

Protein sequence:

>388_residues
MPFLRMNTFSCEVLYQHSGTKARVTRVVTPHGEFITPVFMPVGTRAGVNNMMPSELSAAGTQIILGGNTYHMLCAPGMKV
IENAGGMHRFMGWHGPMLTDSGGFQVFSLSKNKEICSIDESGAHFKLPGSKGIIHMTPEMSLETQKIIGADIIMAFDQCT
PDSCSKDEVSQIMLRTHRWLKQSIDYHQQYPNSRYGYKQALFGIVQGGVFQDLRRESADFVSAMNTDGIAIGGETVGFDM
DTTVEVIRWVYEYLPGNKPRYTMGVGMSPQDLLDVVAEGIDMFDCVAPTRNARHGALYCGKLVREGNWLKFQSEHENGRI
QIKKSCFADDLAPVMESCSCYTCLNYSRSYLHHLSKQKANLFTALASIHNVHVLHDVCNHMRHLIINS

Sequences:

>Translated_388_residues
MPFLRMNTFSCEVLYQHSGTKARVTRVVTPHGEFITPVFMPVGTRAGVNNMMPSELSAAGTQIILGGNTYHMLCAPGMKV
IENAGGMHRFMGWHGPMLTDSGGFQVFSLSKNKEICSIDESGAHFKLPGSKGIIHMTPEMSLETQKIIGADIIMAFDQCT
PDSCSKDEVSQIMLRTHRWLKQSIDYHQQYPNSRYGYKQALFGIVQGGVFQDLRRESADFVSAMNTDGIAIGGETVGFDM
DTTVEVIRWVYEYLPGNKPRYTMGVGMSPQDLLDVVAEGIDMFDCVAPTRNARHGALYCGKLVREGNWLKFQSEHENGRI
QIKKSCFADDLAPVMESCSCYTCLNYSRSYLHHLSKQKANLFTALASIHNVHVLHDVCNHMRHLIINS
>Mature_387_residues
PFLRMNTFSCEVLYQHSGTKARVTRVVTPHGEFITPVFMPVGTRAGVNNMMPSELSAAGTQIILGGNTYHMLCAPGMKVI
ENAGGMHRFMGWHGPMLTDSGGFQVFSLSKNKEICSIDESGAHFKLPGSKGIIHMTPEMSLETQKIIGADIIMAFDQCTP
DSCSKDEVSQIMLRTHRWLKQSIDYHQQYPNSRYGYKQALFGIVQGGVFQDLRRESADFVSAMNTDGIAIGGETVGFDMD
TTVEVIRWVYEYLPGNKPRYTMGVGMSPQDLLDVVAEGIDMFDCVAPTRNARHGALYCGKLVREGNWLKFQSEHENGRIQ
IKKSCFADDLAPVMESCSCYTCLNYSRSYLHHLSKQKANLFTALASIHNVHVLHDVCNHMRHLIINS

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=376, Percent_Identity=36.1702127659575, Blast_Score=227, Evalue=2e-59,
Organism=Homo sapiens, GI13375872, Length=272, Percent_Identity=22.4264705882353, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1786607, Length=365, Percent_Identity=41.6438356164384, Blast_Score=274, Evalue=8e-75,
Organism=Caenorhabditis elegans, GI17544680, Length=364, Percent_Identity=36.8131868131868, Blast_Score=219, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI25146502, Length=285, Percent_Identity=24.9122807017544, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI19920510, Length=374, Percent_Identity=37.4331550802139, Blast_Score=241, Evalue=6e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 43306; Mature: 43175

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
8.5 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
8.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFLRMNTFSCEVLYQHSGTKARVTRVVTPHGEFITPVFMPVGTRAGVNNMMPSELSAAG
CCCEEECCEEEEEEEECCCCEEEEEEEECCCCCEECHHEECCCCCCCCCCCCCHHHHHCC
TQIILGGNTYHMLCAPGMKVIENAGGMHRFMGWHGPMLTDSGGFQVFSLSKNKEICSIDE
CEEEECCCEEEEEECCCHHHHHCCCCHHHHHCCCCCEEECCCCEEEEEECCCCCEEEECC
SGAHFKLPGSKGIIHMTPEMSLETQKIIGADIIMAFDQCTPDSCSKDEVSQIMLRTHRWL
CCCEEECCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
KQSIDYHQQYPNSRYGYKQALFGIVQGGVFQDLRRESADFVSAMNTDGIAIGGETVGFDM
HHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEECCCEECCCC
DTTVEVIRWVYEYLPGNKPRYTMGVGMSPQDLLDVVAEGIDMFDCVAPTRNARHGALYCG
CHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHCCCCHHHHCCCCCCCCCCHHHHH
KLVREGNWLKFQSEHENGRIQIKKSCFADDLAPVMESCSCYTCLNYSRSYLHHLSKQKAN
HHHHCCCEEEEECCCCCCCEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
LFTALASIHNVHVLHDVCNHMRHLIINS
HHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PFLRMNTFSCEVLYQHSGTKARVTRVVTPHGEFITPVFMPVGTRAGVNNMMPSELSAAG
CCEEECCEEEEEEEECCCCEEEEEEEECCCCCEECHHEECCCCCCCCCCCCCHHHHHCC
TQIILGGNTYHMLCAPGMKVIENAGGMHRFMGWHGPMLTDSGGFQVFSLSKNKEICSIDE
CEEEECCCEEEEEECCCHHHHHCCCCHHHHHCCCCCEEECCCCEEEEEECCCCCEEEECC
SGAHFKLPGSKGIIHMTPEMSLETQKIIGADIIMAFDQCTPDSCSKDEVSQIMLRTHRWL
CCCEEECCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
KQSIDYHQQYPNSRYGYKQALFGIVQGGVFQDLRRESADFVSAMNTDGIAIGGETVGFDM
HHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEECCCEECCCC
DTTVEVIRWVYEYLPGNKPRYTMGVGMSPQDLLDVVAEGIDMFDCVAPTRNARHGALYCG
CHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHCCCCHHHHCCCCCCCCCCHHHHH
KLVREGNWLKFQSEHENGRIQIKKSCFADDLAPVMESCSCYTCLNYSRSYLHHLSKQKAN
HHHHCCCEEEEECCCCCCCEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
LFTALASIHNVHVLHDVCNHMRHLIINS
HHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]