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The map label for this gene is rsmB [H]

Identifier: 52842799

GI number: 52842799

Start: 2926079

End: 2927362

Strand: Reverse

Name: rsmB [H]

Synonym: lpg2593

Alternate gene names: 52842799

Gene position: 2927362-2926079 (Counterclockwise)

Preceding gene: 52842800

Following gene: 52842798

Centisome position: 86.16

GC content: 43.07

Gene sequence:

>1284_bases
ATGAAAGTAAATGACAGATTCAACGCCTTAAAAATTTTGACGGCCTTGATAGAAAGCAGAAAAAACCTGTCCTATTTGAT
GTCTCCGCAAGAGATTTCTCCCATGACAAAAGAAATTTGCTATGGATTTTGTCGTCATTATTTTCGTTTACAATCCATAG
CTGATACTTTAATCAATAAGCGACCTAAAGATAATGAGCTATGGATAGTCTTGTTGATAGGTATTTATCAATTGCAGTAT
ATGAATAAGCCGGATTATGCCGTGGTGAAGGAGACAGTGTCTTTACTGGGGCAGGTTAAAAAAAGTTGGGCAAAAGGACT
GGTAAATGCCGTGCTAAGGAATTTTTGCCGTCGAAAAGACCATATTCTGGAAAAGCTGGCAGCAGAGCCGGTTTTTATCT
ATGGACAGCCGAATTGGTTACTTCAACGCCTGCAAAAAGACTGGCCAAACGATTGGCAATTCATCGCTGCAGCGAATGAT
AGACACCCACCCATGACTTTGAGAGTCAATGTGTTGAAACACTCAGTTGATGAGTATCTTGATTTACTAAAAAAATCCGG
GATAGAGGCTTTTAAACACCCTGTCGCACCTGATGGAATTACTTTGGCAATCCCATGTGATGTGATGACGCTTCCTGGTT
TTGCGCAAGGCTCTGTGTCTGTTCAGGATGCCGCGCCTCAATTAGCAGCGTATTTATTGTCTTTAAAACCCGGGCAGCGA
GTACTGGATGCTTGTTGCGCTCCTGGCGGAAAAACATGTCATATTTTGGAGAAAGAGCCTGGTTTGGCTTCCTGTGTTGC
ATTGGATTTAGACCCGCAGCGGTTACAGCGGGTCAGAGATAACTTAAATCGATTGGATTTGCAAGCAACGGTGGTGCAAG
GTGATGTATTGGAACCCAGAAGCTGGTGGGATGGTCATCCATTTGATCGTATTTTACTGGATGCTCCCTGTTCCGCTACA
GGAGTGATTAGACGCCACTCTGATATCAAATTATTACGAACGGATGAAGAGATTTCAGCAATTTCTCAGGTACAATCCAG
TATGTTGAATTCATTATGGCCACTGCTTGCACCAGGTGGTTTAATGGTTTATGCCACATGCTCTATTATGAGTGATGAAA
ATGAGTTGCAAATCGCCCAATTTGTTGCAGAGCATTCGGATTGTCAAGTGAGTGATGGCAAATGGTCCTGGGGAAGGTGG
ACTGGGCATGGTCAACAGATATTGCCTGGGGAGCATAGCATGGATGGCTTTTTTTACAGTGTATTGCGAAAGGATGAAAA
ATGA

Upstream 100 bases:

>100_bases
TGTTAGTGGAAAAGATTCAATTTCCCGGAGCAAAGATCATTTCAGTCAGGGATTGGTTAAATTCGGGTAAAACACAATTG
CATACTGGTTTAATGTTGCA

Downstream 100 bases:

>100_bases
TAATTGATTGGCCTGTAATTATTGTCTTGTTTTGTTTATCCATTCCTGGTGTTTTAATCGTGATGCATCGCCTGGTATAT
TTTCTGCTAGCCGATAATAC

Product: rRNA methyltransferase (SUN protein)

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MKVNDRFNALKILTALIESRKNLSYLMSPQEISPMTKEICYGFCRHYFRLQSIADTLINKRPKDNELWIVLLIGIYQLQY
MNKPDYAVVKETVSLLGQVKKSWAKGLVNAVLRNFCRRKDHILEKLAAEPVFIYGQPNWLLQRLQKDWPNDWQFIAAAND
RHPPMTLRVNVLKHSVDEYLDLLKKSGIEAFKHPVAPDGITLAIPCDVMTLPGFAQGSVSVQDAAPQLAAYLLSLKPGQR
VLDACCAPGGKTCHILEKEPGLASCVALDLDPQRLQRVRDNLNRLDLQATVVQGDVLEPRSWWDGHPFDRILLDAPCSAT
GVIRRHSDIKLLRTDEEISAISQVQSSMLNSLWPLLAPGGLMVYATCSIMSDENELQIAQFVAEHSDCQVSDGKWSWGRW
TGHGQQILPGEHSMDGFFYSVLRKDEK

Sequences:

>Translated_427_residues
MKVNDRFNALKILTALIESRKNLSYLMSPQEISPMTKEICYGFCRHYFRLQSIADTLINKRPKDNELWIVLLIGIYQLQY
MNKPDYAVVKETVSLLGQVKKSWAKGLVNAVLRNFCRRKDHILEKLAAEPVFIYGQPNWLLQRLQKDWPNDWQFIAAAND
RHPPMTLRVNVLKHSVDEYLDLLKKSGIEAFKHPVAPDGITLAIPCDVMTLPGFAQGSVSVQDAAPQLAAYLLSLKPGQR
VLDACCAPGGKTCHILEKEPGLASCVALDLDPQRLQRVRDNLNRLDLQATVVQGDVLEPRSWWDGHPFDRILLDAPCSAT
GVIRRHSDIKLLRTDEEISAISQVQSSMLNSLWPLLAPGGLMVYATCSIMSDENELQIAQFVAEHSDCQVSDGKWSWGRW
TGHGQQILPGEHSMDGFFYSVLRKDEK
>Mature_427_residues
MKVNDRFNALKILTALIESRKNLSYLMSPQEISPMTKEICYGFCRHYFRLQSIADTLINKRPKDNELWIVLLIGIYQLQY
MNKPDYAVVKETVSLLGQVKKSWAKGLVNAVLRNFCRRKDHILEKLAAEPVFIYGQPNWLLQRLQKDWPNDWQFIAAAND
RHPPMTLRVNVLKHSVDEYLDLLKKSGIEAFKHPVAPDGITLAIPCDVMTLPGFAQGSVSVQDAAPQLAAYLLSLKPGQR
VLDACCAPGGKTCHILEKEPGLASCVALDLDPQRLQRVRDNLNRLDLQATVVQGDVLEPRSWWDGHPFDRILLDAPCSAT
GVIRRHSDIKLLRTDEEISAISQVQSSMLNSLWPLLAPGGLMVYATCSIMSDENELQIAQFVAEHSDCQVSDGKWSWGRW
TGHGQQILPGEHSMDGFFYSVLRKDEK

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=350, Percent_Identity=27.1428571428571, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI76150623, Length=350, Percent_Identity=27.1428571428571, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI32698918, Length=174, Percent_Identity=31.6091954022989, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI8922322, Length=305, Percent_Identity=27.5409836065574, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI23199998, Length=305, Percent_Identity=27.5409836065574, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI270288818, Length=305, Percent_Identity=27.5409836065574, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI270288816, Length=305, Percent_Identity=27.5409836065574, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI11545785, Length=183, Percent_Identity=32.7868852459016, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI40316918, Length=187, Percent_Identity=31.5508021390374, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI39995082, Length=181, Percent_Identity=30.939226519337, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI301336155, Length=181, Percent_Identity=30.939226519337, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI2367212, Length=429, Percent_Identity=44.0559440559441, Blast_Score=336, Evalue=2e-93,
Organism=Escherichia coli, GI87081985, Length=249, Percent_Identity=29.3172690763052, Blast_Score=92, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17536757, Length=345, Percent_Identity=30.4347826086957, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI25143471, Length=177, Percent_Identity=30.5084745762712, Blast_Score=65, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6324268, Length=343, Percent_Identity=26.8221574344023, Blast_Score=103, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6319447, Length=261, Percent_Identity=29.8850574712644, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI22024126, Length=285, Percent_Identity=26.6666666666667, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24668781, Length=222, Percent_Identity=31.0810810810811, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21356513, Length=222, Percent_Identity=31.0810810810811, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI19920494, Length=173, Percent_Identity=29.4797687861272, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21355201, Length=187, Percent_Identity=27.2727272727273, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 48216; Mature: 48216

Theoretical pI: Translated: 7.48; Mature: 7.48

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVNDRFNALKILTALIESRKNLSYLMSPQEISPMTKEICYGFCRHYFRLQSIADTLINK
CCCCCHHHHHHHHHHHHHCCCCHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
RPKDNELWIVLLIGIYQLQYMNKPDYAVVKETVSLLGQVKKSWAKGLVNAVLRNFCRRKD
CCCCCCEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HILEKLAAEPVFIYGQPNWLLQRLQKDWPNDWQFIAAANDRHPPMTLRVNVLKHSVDEYL
HHHHHHCCCCEEEEECCHHHHHHHHHCCCCCCEEEEECCCCCCCEEEEHHHHHHHHHHHH
DLLKKSGIEAFKHPVAPDGITLAIPCDVMTLPGFAQGSVSVQDAAPQLAAYLLSLKPGQR
HHHHHCCHHHHHCCCCCCCEEEEECCCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCHH
VLDACCAPGGKTCHILEKEPGLASCVALDLDPQRLQRVRDNLNRLDLQATVVQGDVLEPR
HHHHHCCCCCCEEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHCEEEEEEECCCCCCC
SWWDGHPFDRILLDAPCSATGVIRRHSDIKLLRTDEEISAISQVQSSMLNSLWPLLAPGG
CCCCCCCCCCEEEECCCCCHHHHEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCC
LMVYATCSIMSDENELQIAQFVAEHSDCQVSDGKWSWGRWTGHGQQILPGEHSMDGFFYS
EEEEEEEEEECCCCHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCEECCCCCCHHHHHHH
VLRKDEK
HHHCCCC
>Mature Secondary Structure
MKVNDRFNALKILTALIESRKNLSYLMSPQEISPMTKEICYGFCRHYFRLQSIADTLINK
CCCCCHHHHHHHHHHHHHCCCCHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
RPKDNELWIVLLIGIYQLQYMNKPDYAVVKETVSLLGQVKKSWAKGLVNAVLRNFCRRKD
CCCCCCEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HILEKLAAEPVFIYGQPNWLLQRLQKDWPNDWQFIAAANDRHPPMTLRVNVLKHSVDEYL
HHHHHHCCCCEEEEECCHHHHHHHHHCCCCCCEEEEECCCCCCCEEEEHHHHHHHHHHHH
DLLKKSGIEAFKHPVAPDGITLAIPCDVMTLPGFAQGSVSVQDAAPQLAAYLLSLKPGQR
HHHHHCCHHHHHCCCCCCCEEEEECCCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCHH
VLDACCAPGGKTCHILEKEPGLASCVALDLDPQRLQRVRDNLNRLDLQATVVQGDVLEPR
HHHHHCCCCCCEEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHCEEEEEEECCCCCCC
SWWDGHPFDRILLDAPCSATGVIRRHSDIKLLRTDEEISAISQVQSSMLNSLWPLLAPGG
CCCCCCCCCCEEEECCCCCHHHHEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCC
LMVYATCSIMSDENELQIAQFVAEHSDCQVSDGKWSWGRWTGHGQQILPGEHSMDGFFYS
EEEEEEEEEECCCCHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCEECCCCCCHHHHHHH
VLRKDEK
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]