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The map label for this gene is lcfA [H]

Identifier: 52841361

GI number: 52841361

Start: 1240718

End: 1242226

Strand: Reverse

Name: lcfA [H]

Synonym: lpg1127

Alternate gene names: 52841361

Gene position: 1242226-1240718 (Counterclockwise)

Preceding gene: 52841364

Following gene: 52841358

Centisome position: 36.56

GC content: 37.51

Gene sequence:

>1509_bases
ATGACTAAAAAAAGAATAGACAGGCTTATTTTTTCAGAAGTTGAATGCAATATGAAAAAATGCTCTATGGTTATTGCAGG
CAAACCCATATCCTATGAGAAATTGTTTGGTACAGTGAATCGTATTGCAATTCACTTTCAAAATAAAGCACTCTCTGGAA
GCCGTATCGCTTTTATGTTGCCCAATAGTCTTGAAATTATAGCCATTTACCTGGCCTGTTTTCAATCAGGATGTATTGCC
ATGCCTGTTAATCGCCGCTATGCGCCACCTGAGCTTGAAAAGGTATTGCGCGATGCCCAACCAATTTATTTAATTATCGA
AGCAAGTAAATTATTTTTGCTAGAGAAAATCAATTGGTCAGCGACAGGAATTAAAAAAATTTTTGTCCTGGGGGAAAATC
AGAATTATCCTTATTTTTCTTTTAATGAATTATTAAATGAACCAGCGAATTGCCCAAAAAATGGTATAGATTATAAAGCG
CCTGCAGTAATTTTTTATACCTCCGGTTCAACGGGGGAACCCAAAGGAGTTGTTCATACGCTTTCTTCCATTGAAGCGAT
GCTTGACTCGACTTCATTGGCACTGGAAAACATTACTGCTGACGACAAAATGATAATCTGTGAGCCACAATGCCATGTAA
GTGGATTTATCGAAACATTCTCTACATTATCACAAGAAGGTACCGCGTTAGTTCATGATGGTTTTGATATGGATTGGTAC
CTTGCGACAGTGCGACATGAAAAACCAACACTGGCCGTTCATCATATTGATACCTATATAAAGTTATTAGACTCTGGACG
CTGTAATACAAAAACATTTCAATCATTTCGTGGAATTTATACAGGTGGGGATACACTACCTGTGGCAGTCCAGGACAAGT
TTCTGGCTTGCGCTGGTAAACCAATTCAAGTGGGTTATGGAATGACGGAAGCCATTTGGCTAACAGTCTGCAGAACCCCT
GATTTGACTCAAAAATCCTGTATTGGCAAACCGGTGAATGGGGTAACATTACGCCTTGTAAACGCAGCTGGAGAAGACGT
ACGTTGTGGTGAAACAGGAGAAATACTGGTAAAAGGTGATATGGTGATGCATAGTTATTGGAATAATCCTGAAGAAACCC
GCAAGGCATTTATAAAAGGTTGGTTTAAAACAGGAGATTGCGGAATTCAGGATGAACAAGGTTATTTTTATTATAAAGGT
CGGATTAAAAACATTATTATCCGCAATACATCCAATATTATGCCTGGTGAAGTGGAAGCAACCATTTATAAACATCCAGC
AATTAGCGCAGCGGCTGTGATTGGTGTACCTGACGAAGCAGAAGGAGAAGTACCAATTGCTTTTGTAGTAGTAAAAAAGA
GTAACCAATTGACAAAAGAAGAATTATATTCTTTTTTAATTGAGCAAATTGCTCAATATAAAATACCTGCAAAAATATAC
TTTATTGATGAGATGCCCTTGACCAATAGTGGAAAAATTAATCATAAGAAACTGTATGATTATCTTTAA

Upstream 100 bases:

>100_bases
ACGCTTTATAAAATCCATTTTTTTCCCATTTTTAGCCCATAATTATTAATTATTTCCCATAATATAAAAAGTAACAAATG
AATCTATCCAGGAAAACATA

Downstream 100 bases:

>100_bases
AATTAAAAAATACACAGTTTACATGAGGTCAATAAGCATCACAAAAATATTTATGACCGAGCGATAAAATAAAAACAGCC
CCTTTATGCAAGGGGCTATT

Product: acyl CoA synthetase, long chain fatty acid:CoA ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 502; Mature: 501

Protein sequence:

>502_residues
MTKKRIDRLIFSEVECNMKKCSMVIAGKPISYEKLFGTVNRIAIHFQNKALSGSRIAFMLPNSLEIIAIYLACFQSGCIA
MPVNRRYAPPELEKVLRDAQPIYLIIEASKLFLLEKINWSATGIKKIFVLGENQNYPYFSFNELLNEPANCPKNGIDYKA
PAVIFYTSGSTGEPKGVVHTLSSIEAMLDSTSLALENITADDKMIICEPQCHVSGFIETFSTLSQEGTALVHDGFDMDWY
LATVRHEKPTLAVHHIDTYIKLLDSGRCNTKTFQSFRGIYTGGDTLPVAVQDKFLACAGKPIQVGYGMTEAIWLTVCRTP
DLTQKSCIGKPVNGVTLRLVNAAGEDVRCGETGEILVKGDMVMHSYWNNPEETRKAFIKGWFKTGDCGIQDEQGYFYYKG
RIKNIIIRNTSNIMPGEVEATIYKHPAISAAAVIGVPDEAEGEVPIAFVVVKKSNQLTKEELYSFLIEQIAQYKIPAKIY
FIDEMPLTNSGKINHKKLYDYL

Sequences:

>Translated_502_residues
MTKKRIDRLIFSEVECNMKKCSMVIAGKPISYEKLFGTVNRIAIHFQNKALSGSRIAFMLPNSLEIIAIYLACFQSGCIA
MPVNRRYAPPELEKVLRDAQPIYLIIEASKLFLLEKINWSATGIKKIFVLGENQNYPYFSFNELLNEPANCPKNGIDYKA
PAVIFYTSGSTGEPKGVVHTLSSIEAMLDSTSLALENITADDKMIICEPQCHVSGFIETFSTLSQEGTALVHDGFDMDWY
LATVRHEKPTLAVHHIDTYIKLLDSGRCNTKTFQSFRGIYTGGDTLPVAVQDKFLACAGKPIQVGYGMTEAIWLTVCRTP
DLTQKSCIGKPVNGVTLRLVNAAGEDVRCGETGEILVKGDMVMHSYWNNPEETRKAFIKGWFKTGDCGIQDEQGYFYYKG
RIKNIIIRNTSNIMPGEVEATIYKHPAISAAAVIGVPDEAEGEVPIAFVVVKKSNQLTKEELYSFLIEQIAQYKIPAKIY
FIDEMPLTNSGKINHKKLYDYL
>Mature_501_residues
TKKRIDRLIFSEVECNMKKCSMVIAGKPISYEKLFGTVNRIAIHFQNKALSGSRIAFMLPNSLEIIAIYLACFQSGCIAM
PVNRRYAPPELEKVLRDAQPIYLIIEASKLFLLEKINWSATGIKKIFVLGENQNYPYFSFNELLNEPANCPKNGIDYKAP
AVIFYTSGSTGEPKGVVHTLSSIEAMLDSTSLALENITADDKMIICEPQCHVSGFIETFSTLSQEGTALVHDGFDMDWYL
ATVRHEKPTLAVHHIDTYIKLLDSGRCNTKTFQSFRGIYTGGDTLPVAVQDKFLACAGKPIQVGYGMTEAIWLTVCRTPD
LTQKSCIGKPVNGVTLRLVNAAGEDVRCGETGEILVKGDMVMHSYWNNPEETRKAFIKGWFKTGDCGIQDEQGYFYYKGR
IKNIIIRNTSNIMPGEVEATIYKHPAISAAAVIGVPDEAEGEVPIAFVVVKKSNQLTKEELYSFLIEQIAQYKIPAKIYF
IDEMPLTNSGKINHKKLYDYL

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=498, Percent_Identity=27.9116465863454, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI187761343, Length=498, Percent_Identity=27.9116465863454, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI38505220, Length=488, Percent_Identity=26.844262295082, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI122937307, Length=484, Percent_Identity=25.8264462809917, Blast_Score=142, Evalue=9e-34,
Organism=Homo sapiens, GI156151445, Length=522, Percent_Identity=27.7777777777778, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI42544132, Length=489, Percent_Identity=24.9488752556237, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI157311624, Length=466, Percent_Identity=25.5364806866953, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI157311622, Length=466, Percent_Identity=25.5364806866953, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI58082049, Length=466, Percent_Identity=25.3218884120172, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI115511026, Length=503, Percent_Identity=25.0497017892644, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI13375727, Length=529, Percent_Identity=20.7939508506616, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI28416953, Length=122, Percent_Identity=35.2459016393443, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI8923896, Length=380, Percent_Identity=25.5263157894737, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI253970489, Length=380, Percent_Identity=25.5263157894737, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI1788107, Length=519, Percent_Identity=26.7822736030828, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI145693145, Length=466, Percent_Identity=28.5407725321888, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI221142682, Length=482, Percent_Identity=26.7634854771784, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI1786810, Length=252, Percent_Identity=28.5714285714286, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1789201, Length=526, Percent_Identity=23.1939163498099, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1786529, Length=522, Percent_Identity=21.264367816092, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1788595, Length=339, Percent_Identity=25.3687315634218, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1790505, Length=527, Percent_Identity=21.6318785578748, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1786801, Length=505, Percent_Identity=22.5742574257426, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17558820, Length=500, Percent_Identity=27.2, Blast_Score=168, Evalue=5e-42,
Organism=Caenorhabditis elegans, GI32563687, Length=464, Percent_Identity=27.5862068965517, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17559526, Length=497, Percent_Identity=26.7605633802817, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17557194, Length=531, Percent_Identity=26.3653483992467, Blast_Score=145, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI17531443, Length=458, Percent_Identity=25.5458515283843, Blast_Score=139, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17538037, Length=492, Percent_Identity=27.2357723577236, Blast_Score=137, Evalue=9e-33,
Organism=Caenorhabditis elegans, GI71994703, Length=347, Percent_Identity=29.971181556196, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI71994694, Length=347, Percent_Identity=29.971181556196, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI71994690, Length=347, Percent_Identity=29.971181556196, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17560308, Length=517, Percent_Identity=23.4042553191489, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17560140, Length=343, Percent_Identity=25.9475218658892, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI71985884, Length=373, Percent_Identity=27.3458445040214, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71996755, Length=503, Percent_Identity=24.0556660039761, Blast_Score=98, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI71983001, Length=381, Percent_Identity=23.6220472440945, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71982997, Length=381, Percent_Identity=23.6220472440945, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319699, Length=505, Percent_Identity=27.1287128712871, Blast_Score=160, Evalue=5e-40,
Organism=Saccharomyces cerevisiae, GI6319264, Length=528, Percent_Identity=23.2954545454545, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6323182, Length=193, Percent_Identity=30.0518134715026, Blast_Score=84, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6323903, Length=144, Percent_Identity=32.6388888888889, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI21355181, Length=461, Percent_Identity=27.3318872017354, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI18859661, Length=506, Percent_Identity=25.6916996047431, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24581924, Length=513, Percent_Identity=23.3918128654971, Blast_Score=123, Evalue=4e-28,
Organism=Drosophila melanogaster, GI19922652, Length=493, Percent_Identity=24.7464503042596, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24653035, Length=518, Percent_Identity=23.1660231660232, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI21356947, Length=515, Percent_Identity=23.3009708737864, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI21356441, Length=450, Percent_Identity=23.1111111111111, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI24648260, Length=343, Percent_Identity=26.2390670553936, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI21358303, Length=488, Percent_Identity=22.5409836065574, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI161076582, Length=517, Percent_Identity=22.4371373307544, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24648253, Length=506, Percent_Identity=21.7391304347826, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24648255, Length=506, Percent_Identity=21.7391304347826, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24656500, Length=352, Percent_Identity=23.2954545454545, Blast_Score=102, Evalue=8e-22,
Organism=Drosophila melanogaster, GI20130357, Length=506, Percent_Identity=21.7391304347826, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI281365686, Length=516, Percent_Identity=21.1240310077519, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24648257, Length=463, Percent_Identity=21.8142548596112, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI21355225, Length=444, Percent_Identity=21.1711711711712, Blast_Score=80, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 56095; Mature: 55964

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKRIDRLIFSEVECNMKKCSMVIAGKPISYEKLFGTVNRIAIHFQNKALSGSRIAFML
CCHHHHHHHHHHHHHCCHHHCEEEEECCCCCHHHHHCCEEEEEEEEECCCCCCCEEEEEC
PNSLEIIAIYLACFQSGCIAMPVNRRYAPPELEKVLRDAQPIYLIIEASKLFLLEKINWS
CCCHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHCCCCEEEEEECCEEEEEEECCCC
ATGIKKIFVLGENQNYPYFSFNELLNEPANCPKNGIDYKAPAVIFYTSGSTGEPKGVVHT
CCCEEEEEEEECCCCCCEEEHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHH
LSSIEAMLDSTSLALENITADDKMIICEPQCHVSGFIETFSTLSQEGTALVHDGFDMDWY
HHHHHHHHCCHHHHEEECCCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCCEEE
LATVRHEKPTLAVHHIDTYIKLLDSGRCNTKTFQSFRGIYTGGDTLPVAVQDKFLACAGK
EEEEECCCCEEEEEHHHHHHHHHCCCCCCHHHHHHHCCEEECCCEEEEEECCCEEEECCC
PIQVGYGMTEAIWLTVCRTPDLTQKSCIGKPVNGVTLRLVNAAGEDVRCGETGEILVKGD
EEEECCCCCEEEEEEEECCCCCCHHHHCCCCCCCEEEEEEECCCCCEECCCCCCEEEECC
MVMHSYWNNPEETRKAFIKGWFKTGDCGIQDEQGYFYYKGRIKNIIIRNTSNIMPGEVEA
EEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCEEEEEEEECCCCCCCCCEEE
TIYKHPAISAAAVIGVPDEAEGEVPIAFVVVKKSNQLTKEELYSFLIEQIAQYKIPAKIY
EEEECCCCCEEEEEECCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEE
FIDEMPLTNSGKINHKKLYDYL
EEECCCCCCCCCCCHHHHHCCC
>Mature Secondary Structure 
TKKRIDRLIFSEVECNMKKCSMVIAGKPISYEKLFGTVNRIAIHFQNKALSGSRIAFML
CHHHHHHHHHHHHHCCHHHCEEEEECCCCCHHHHHCCEEEEEEEEECCCCCCCEEEEEC
PNSLEIIAIYLACFQSGCIAMPVNRRYAPPELEKVLRDAQPIYLIIEASKLFLLEKINWS
CCCHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHCCCCEEEEEECCEEEEEEECCCC
ATGIKKIFVLGENQNYPYFSFNELLNEPANCPKNGIDYKAPAVIFYTSGSTGEPKGVVHT
CCCEEEEEEEECCCCCCEEEHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHH
LSSIEAMLDSTSLALENITADDKMIICEPQCHVSGFIETFSTLSQEGTALVHDGFDMDWY
HHHHHHHHCCHHHHEEECCCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCCEEE
LATVRHEKPTLAVHHIDTYIKLLDSGRCNTKTFQSFRGIYTGGDTLPVAVQDKFLACAGK
EEEEECCCCEEEEEHHHHHHHHHCCCCCCHHHHHHHCCEEECCCEEEEEECCCEEEECCC
PIQVGYGMTEAIWLTVCRTPDLTQKSCIGKPVNGVTLRLVNAAGEDVRCGETGEILVKGD
EEEECCCCCEEEEEEEECCCCCCHHHHCCCCCCCEEEEEEECCCCCEECCCCCCEEEECC
MVMHSYWNNPEETRKAFIKGWFKTGDCGIQDEQGYFYYKGRIKNIIIRNTSNIMPGEVEA
EEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCEEEEEEEECCCCCCCCCEEE
TIYKHPAISAAAVIGVPDEAEGEVPIAFVVVKKSNQLTKEELYSFLIEQIAQYKIPAKIY
EEEECCCCCEEEEEECCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEE
FIDEMPLTNSGKINHKKLYDYL
EEECCCCCCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]