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The map label for this gene is mutY [H]

Identifier: 52841134

GI number: 52841134

Start: 973918

End: 974985

Strand: Reverse

Name: mutY [H]

Synonym: lpg0899

Alternate gene names: 52841134

Gene position: 974985-973918 (Counterclockwise)

Preceding gene: 52841135

Following gene: 52841133

Centisome position: 28.69

GC content: 38.86

Gene sequence:

>1068_bases
TTGAGTACAAACTCTTTAAATCAACTTTTCAGTCAATTACTGCTTGATTGGTATGACCTGCATGGACGCAAAGATTTGCC
ATGGCAATTACCCCGCTCTCCCTACAGAGTTTGGGTATCTGAAATCATGTTACAACAAACTCAAGTTCAAACTGTTATTC
CCTACTTTAATCGTTTTATAGAACATTTCCCTGATATTTTTTTGCTTGCTAATGCAGATGAGGATGAAGTGCTCTCTCTA
TGGTCGGGGTTAGGCTATTACAGTCGCGCCAGAAATCTTCACAACACAGCAAAAATAATTAGTGACCAGTATAATGGCGT
ATTTCCTGAAGATCTGAACATTTTAGTTCAACTGCCAGGAATAGGACCATCGACAGCAGCAGCGATACTTTCACAAGCTT
TTAATAAACCAGCTGCTATTTTAGATGGGAATGTGAAGCGAGTTTTGTCTCGATTTTTTTTAATCGAAGGCTGGCCAGAA
CAAGCCCAGGTTAAAAAAAAACTATGGGGGCTTGCGTCTTCTTGTATGCCCAATGAACGGTGTGCAGATTATACACAAGC
AATTATGGACTTAGGTGCAACATGCTGCACAAATAAAAATCCTCAGTGTTTGCGTTGTCCTGTTAAAAATCATTGCCTGG
CCTTTCATAATAAAAAACAACATCTTTATCCAACAAAAAAAATAAAGAAACAGCGCCCAATATTATCCCAACAGTTCTTG
GTGTTGCATAATAGACAAAATCAGGTTTATCTGGAAAAAAGACCTCCTACAGGGTTATGGGGAGGCTTATGGTGTTTACC
CAGTATAAATAATCAAACTTGCCCAATTGAGCATATCCAACTCTTTTACAGGTTGCAGGGAGATAGCCCAAAGCTAATTA
CCCGGTTTAAACACAGCTTTAGCCATTTTCATTTGGAAATAACAGCATTGAGCATAAGAATAGAATCTACGAATAATTTT
ATCTCTGAATCCAGAGGACAATGGTTTACAAAAGAAACCTTGCCAACATTAGGTCTTGCCAAACCAACCACCTTGATACT
ATCCAAATTAATGGAGACTGGCGCCTAG

Upstream 100 bases:

>100_bases
ATTTTAAAGCAATTAGCCCTGTATTAAGTTCGTTAGCGATCAAATCGGCTCTGACAAAACCTTTAAAAATAATACAAAAA
CCACTTAAAGAGCTAATCCA

Downstream 100 bases:

>100_bases
CGAAGAACCGTTTTAAAACCACCAAAAGGTGTATAAACTGTTAAGTCCGCATAAGCCAATATCAATCTAACAAAATCTTT
ACAAATAATTTATTTATTCA

Product: A/G specific adenine glycosylase

Products: Adenine [C]

Alternate protein names: NA

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MSTNSLNQLFSQLLLDWYDLHGRKDLPWQLPRSPYRVWVSEIMLQQTQVQTVIPYFNRFIEHFPDIFLLANADEDEVLSL
WSGLGYYSRARNLHNTAKIISDQYNGVFPEDLNILVQLPGIGPSTAAAILSQAFNKPAAILDGNVKRVLSRFFLIEGWPE
QAQVKKKLWGLASSCMPNERCADYTQAIMDLGATCCTNKNPQCLRCPVKNHCLAFHNKKQHLYPTKKIKKQRPILSQQFL
VLHNRQNQVYLEKRPPTGLWGGLWCLPSINNQTCPIEHIQLFYRLQGDSPKLITRFKHSFSHFHLEITALSIRIESTNNF
ISESRGQWFTKETLPTLGLAKPTTLILSKLMETGA

Sequences:

>Translated_355_residues
MSTNSLNQLFSQLLLDWYDLHGRKDLPWQLPRSPYRVWVSEIMLQQTQVQTVIPYFNRFIEHFPDIFLLANADEDEVLSL
WSGLGYYSRARNLHNTAKIISDQYNGVFPEDLNILVQLPGIGPSTAAAILSQAFNKPAAILDGNVKRVLSRFFLIEGWPE
QAQVKKKLWGLASSCMPNERCADYTQAIMDLGATCCTNKNPQCLRCPVKNHCLAFHNKKQHLYPTKKIKKQRPILSQQFL
VLHNRQNQVYLEKRPPTGLWGGLWCLPSINNQTCPIEHIQLFYRLQGDSPKLITRFKHSFSHFHLEITALSIRIESTNNF
ISESRGQWFTKETLPTLGLAKPTTLILSKLMETGA
>Mature_354_residues
STNSLNQLFSQLLLDWYDLHGRKDLPWQLPRSPYRVWVSEIMLQQTQVQTVIPYFNRFIEHFPDIFLLANADEDEVLSLW
SGLGYYSRARNLHNTAKIISDQYNGVFPEDLNILVQLPGIGPSTAAAILSQAFNKPAAILDGNVKRVLSRFFLIEGWPEQ
AQVKKKLWGLASSCMPNERCADYTQAIMDLGATCCTNKNPQCLRCPVKNHCLAFHNKKQHLYPTKKIKKQRPILSQQFLV
LHNRQNQVYLEKRPPTGLWGGLWCLPSINNQTCPIEHIQLFYRLQGDSPKLITRFKHSFSHFHLEITALSIRIESTNNFI
SESRGQWFTKETLPTLGLAKPTTLILSKLMETGA

Specific function: Adenine glycosylase active on G-A mispairs. MutY also corrects error-prone DNA synthesis past GO lesions which are due to the oxidatively damaged form of guanine:7,8-dihydro-8- oxoguanine (8-oxo-dGTP) [H]

COG id: COG1194

COG function: function code L; A/G-specific DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI115298648, Length=395, Percent_Identity=34.1772151898734, Blast_Score=191, Evalue=6e-49,
Organism=Homo sapiens, GI6912520, Length=395, Percent_Identity=34.1772151898734, Blast_Score=191, Evalue=6e-49,
Organism=Homo sapiens, GI115298650, Length=395, Percent_Identity=34.1772151898734, Blast_Score=191, Evalue=7e-49,
Organism=Homo sapiens, GI190358497, Length=395, Percent_Identity=34.1772151898734, Blast_Score=191, Evalue=7e-49,
Organism=Homo sapiens, GI115298654, Length=395, Percent_Identity=34.1772151898734, Blast_Score=191, Evalue=8e-49,
Organism=Homo sapiens, GI115298652, Length=395, Percent_Identity=34.1772151898734, Blast_Score=191, Evalue=8e-49,
Organism=Escherichia coli, GI1789331, Length=346, Percent_Identity=45.9537572254335, Blast_Score=326, Evalue=1e-90,
Organism=Escherichia coli, GI1787920, Length=127, Percent_Identity=32.2834645669291, Blast_Score=65, Evalue=5e-12,
Organism=Drosophila melanogaster, GI45550361, Length=211, Percent_Identity=26.0663507109005, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170
- InterPro:   IPR005760
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: 3.2.2.- [C]

Molecular weight: Translated: 40723; Mature: 40591

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTNSLNQLFSQLLLDWYDLHGRKDLPWQLPRSPYRVWVSEIMLQQTQVQTVIPYFNRFI
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EHFPDIFLLANADEDEVLSLWSGLGYYSRARNLHNTAKIISDQYNGVFPEDLNILVQLPG
HHCCCEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECC
IGPSTAAAILSQAFNKPAAILDGNVKRVLSRFFLIEGWPEQAQVKKKLWGLASSCMPNER
CCHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHH
CADYTQAIMDLGATCCTNKNPQCLRCPVKNHCLAFHNKKQHLYPTKKIKKQRPILSQQFL
HHHHHHHHHHCCHHHCCCCCCCEEECCHHHHHEEECCCCCCCCCHHHHHHHCCHHHHHHH
VLHNRQNQVYLEKRPPTGLWGGLWCLPSINNQTCPIEHIQLFYRLQGDSPKLITRFKHSF
EEECCCCEEEEEECCCCCCCCCEEECCCCCCCCCCHHHHHEEHEECCCCHHHHHHHHHHH
SHFHLEITALSIRIESTNNFISESRGQWFTKETLPTLGLAKPTTLILSKLMETGA
CEEEEEEEEEEEEEECCCCHHHHCCCCEEEHHCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
STNSLNQLFSQLLLDWYDLHGRKDLPWQLPRSPYRVWVSEIMLQQTQVQTVIPYFNRFI
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EHFPDIFLLANADEDEVLSLWSGLGYYSRARNLHNTAKIISDQYNGVFPEDLNILVQLPG
HHCCCEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECC
IGPSTAAAILSQAFNKPAAILDGNVKRVLSRFFLIEGWPEQAQVKKKLWGLASSCMPNER
CCHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHH
CADYTQAIMDLGATCCTNKNPQCLRCPVKNHCLAFHNKKQHLYPTKKIKKQRPILSQQFL
HHHHHHHHHHCCHHHCCCCCCCEEECCHHHHHEEECCCCCCCCCHHHHHHHCCHHHHHHH
VLHNRQNQVYLEKRPPTGLWGGLWCLPSINNQTCPIEHIQLFYRLQGDSPKLITRFKHSF
EEECCCCEEEEEECCCCCCCCCEEECCCCCCCCCCHHHHHEEHEECCCCHHHHHHHHHHH
SHFHLEITALSIRIESTNNFISESRGQWFTKETLPTLGLAKPTTLILSKLMETGA
CEEEEEEEEEEEEEECCCCHHHHCCCCEEEHHCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 4Fe-4S Cluster [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Guanine-adenine mispair [C]

Specific reaction: Catalyzes the excision of adenine from a guanine-adenine mispair [C]

General reaction: Hydrolase; Glycosylases; Hydrolysing N-glycosyl compounds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2197596; 2001994; 9278503; 9846876 [H]