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The map label for this gene is yydA

Identifier: 52788000

GI number: 52788000

Start: 4175673

End: 4176152

Strand: Reverse

Name: yydA

Synonym: BLi04324

Alternate gene names: 52788000

Gene position: 4176152-4175673 (Counterclockwise)

Preceding gene: 52788001

Following gene: 52787996

Centisome position: 98.9

GC content: 46.25

Gene sequence:

>480_bases
GTGAATATATCAATCGTAGCAATCGGAAAACTAAAAGAAAAATATTTAAAACAAGGAATCGATGAATACATAAAACGGCT
TTCGGCATACGCAAAAGTCGACATCATCGAACTTCCGGACGAAAAAGCACCGGAAAACCTAAGCGACCAGGACATGAAAA
TCGTCAAAGACAAAGAAGGCGAACGAATTCTCTCCAAAATCAGCCCAGACGCCCACGTCATCGCCCTGGCGATCGAAGGA
AAAATGAAAAGTTCGGAAGAATTAGCCGATAATATGGATAGACTCGCCACATATGGAAAGAGCAAGGCAACCTTCGTCAT
CGGCGGCTCGCTCGGCTTAAGCGACGCCGTCCTGAAGCGCGCAGATGAGAAGCTGTCGTTTTCACGGATGACGTTCCCGC
ATCAGCTGATGAGACTGATTTTGCTCGAGCAGGTTTATCGGGCTTTTAGGATTAATCGAGGGGAACCGTACCATAAATAG

Upstream 100 bases:

>100_bases
ATATAGTGGGGAACGAATGATCGGACACTAAATACACAACCTTTATCCACAATTGTGGATAATGCATCTGAATAACTTGT
TCTTAAAGTGGGGATGACCT

Downstream 100 bases:

>100_bases
AGGCAAAAAATGATGTAAATATGGTGGTATTTTGTTGTCGGAAGCGGGTATTGGCTTTGGAAAACCATTTGCATTATCCG
TTTCTCTATACAACATGTGG

Product: rRNA large subunit methyltransferase

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MNISIVAIGKLKEKYLKQGIDEYIKRLSAYAKVDIIELPDEKAPENLSDQDMKIVKDKEGERILSKISPDAHVIALAIEG
KMKSSEELADNMDRLATYGKSKATFVIGGSLGLSDAVLKRADEKLSFSRMTFPHQLMRLILLEQVYRAFRINRGEPYHK

Sequences:

>Translated_159_residues
MNISIVAIGKLKEKYLKQGIDEYIKRLSAYAKVDIIELPDEKAPENLSDQDMKIVKDKEGERILSKISPDAHVIALAIEG
KMKSSEELADNMDRLATYGKSKATFVIGGSLGLSDAVLKRADEKLSFSRMTFPHQLMRLILLEQVYRAFRINRGEPYHK
>Mature_159_residues
MNISIVAIGKLKEKYLKQGIDEYIKRLSAYAKVDIIELPDEKAPENLSDQDMKIVKDKEGERILSKISPDAHVIALAIEG
KMKSSEELADNMDRLATYGKSKATFVIGGSLGLSDAVLKRADEKLSFSRMTFPHQLMRLILLEQVYRAFRINRGEPYHK

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=159, Percent_Identity=32.7044025157233, Blast_Score=97, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_BACLD (Q65CS8)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_081393.1
- RefSeq:   YP_093829.1
- ProteinModelPortal:   Q65CS8
- SMR:   Q65CS8
- STRING:   Q65CS8
- EnsemblBacteria:   EBBACT00000056108
- EnsemblBacteria:   EBBACT00000059058
- GeneID:   3027943
- GeneID:   3097423
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi04324
- KEGG:   bli:BL02382
- NMPDR:   fig|279010.5.peg.4194
- eggNOG:   COG1576
- GeneTree:   EBGT00050000003035
- HOGENOM:   HBG622807
- OMA:   TANHPYH
- ProtClustDB:   PRK00103
- BioCyc:   BLIC279010-1:BLI04324-MONOMER
- BioCyc:   BLIC279010:BL02382-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR016051
- InterPro:   IPR003742
- PIRSF:   PIRSF004505
- TIGRFAMs:   TIGR00246

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 17997; Mature: 17997

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNISIVAIGKLKEKYLKQGIDEYIKRLSAYAKVDIIELPDEKAPENLSDQDMKIVKDKEG
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCHHHHHCCCH
ERILSKISPDAHVIALAIEGKMKSSEELADNMDRLATYGKSKATFVIGGSLGLSDAVLKR
HHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHH
ADEKLSFSRMTFPHQLMRLILLEQVYRAFRINRGEPYHK
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNISIVAIGKLKEKYLKQGIDEYIKRLSAYAKVDIIELPDEKAPENLSDQDMKIVKDKEG
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCHHHHHCCCH
ERILSKISPDAHVIALAIEGKMKSSEELADNMDRLATYGKSKATFVIGGSLGLSDAVLKR
HHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHH
ADEKLSFSRMTFPHQLMRLILLEQVYRAFRINRGEPYHK
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA