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The map label for this gene is lacA [H]

Identifier: 52787954

GI number: 52787954

Start: 4120139

End: 4122193

Strand: Reverse

Name: lacA [H]

Synonym: BLi04277

Alternate gene names: 52787954

Gene position: 4122193-4120139 (Counterclockwise)

Preceding gene: 52787955

Following gene: 52787953

Centisome position: 97.62

GC content: 49.2

Gene sequence:

>2055_bases
ATGCCAAAAATTTATACGACCCAAGCAAGATACATGCTTCACGGGGGAGACTACAATCCGGATCAGTGGCTTGACCGTCC
GGATATTCTTGCAGATGACATCAAGCTGATGAAGCTTGCCCATACGAATACATTTTCAGTGGGGATTTTTTCATGGAGCG
CCCTTGAGCCGGAGGAAGGCGTCTATACGTTTGAATGGCTGGATGATATTTTTGAGAGCATTCACCGGAACGGCGGCCGG
ATTATATTGGCGACACCGAGCGGCGCCCGTCCTGCTTGGCTGTCGCAAAAGTATCCGGAAGTGCTGCGCGTGAATGCTGA
ACGCGTCAAACAGCTGCACGGCGGAAGGCACAATCATTGCTTCACTTCATACGTATACCGTGAAAAAACGAAAGAGATCA
ACCGCATGCTGGCTGAAAGGTACGGCAGCCAGCATGCCCTTTTGATGTGGCATGTTTCCAACGAATACGGAGGAGAATGC
CATTGTGATCAATGTCAGCACGCCTTCAGAGACTGGCTGAAAAAGAAGTACAACCATGATATTAAATCTTTGAATGACGC
ATGGTGGACGCCTTTTTGGAGCCACACCTTCAACGATTGGTCACAGATTGAATCGCCGTCTCCGATCGGGGAAAATGCGG
TGCACGGCTTGAATTTAGACTGGCGACGCTTCGTCACTGACCAGACGATCTCTTTTTTTCAAAATGAAATCGTTCCGTTA
AAGGAAATCACGCCGAATATTCCGATTACGACCAATTTTATGGCGGACACGCATGATTTAATTCCGTTTCAAGGGCTGGA
CTACAGCAAGTTTGCCAAGCACCTGGACGTGATCAGCTGGGACGCGTATCCCGCCTGGCACAACGATTGGGAAAGCACAG
CAGATTTGGCCATGAAAGTCGGCTTTATCAATGATTTGTACCGCAGCCTGAAACAGCAGCCGTTTCTCTTAATGGAATCG
ACGCCAAGCGCTGTCAATTGGCACGATTTTAATAAAGCGAAACGGCCGGGCATGCACCTCCTGTCTTCTGTGCAGATGAT
CGCACACGGATCTGACAGCATCCTCTATTTTCAATGGCGGAAATCCCGCGGCTCATCGGAAAAATTTCATGGTGCGGTTG
TCGGCCATGACAATTGTTCCGAGAACCGCGTGTTTAAAGAAGTGGCAAAAGTCGGGCAAACCCTTGAAGCGCTGTCTGAA
GTCACCGGAACGATCCGGCCTGCGGATGTCGCGATTCTCTACGATTGGGAAAACCATTGGGCGCTCCAAGATGCACAAGG
ATTCGGGATGAAAACCAAGCGGTATCCCCAAACCCTGCACGAGCACTACCGCGCGTTTTGGGAGCGTGATATTCCAGTCG
ACGTGATTACGAAGGAACAGGATTTTTCATCGTACCGGCTGCTGATCGTTCCGATGCTTTACTTAGCAAGCGAAGAAACA
ATCGCGCGCTTAAAGGCATTTGCTGCCAACGGGGGCACATTGGTGATGACCTATATTAGCGGAATCGTCAACGAATCTGA
TCTGACCTATTTAGGCGGATGGCCAAAAGATCTGCAAGAGATGTTTGGCATGGAGCCTGTTGAAACCGATACACTCTATC
CGGGCGATAAAAATGCGGTCCGTTATCAAAACCGGTCGTATGAGCTGAAAGATTACGCGACCGTGCTGAAGCTCAGTACG
GCAGATCCCGAGGGATTCTATGAGGACGACTTTTATGCGGACACGACGGCTGTGACGAGCCATCCATACAAACAGGGGAA
AACGTATTATATCGGTGCGCGTTTAAGCAGCCAATTTCATCGGGATTTTTATGGAACATTGATTAAAGAGCTAGCGATTC
AACCGGCTCTTGACGTGAAACATCAGCCTGGCGTCTCCGTTCAAGTCAGACAGGATGAGGAAAATGACTATATTTTCATC
ATGAATTTCACGGAAAAACGTCAGCCCGTCGTGTTGGCGTCGGCAGTTAAAGATATGCTGACCGGAGAAACGCTGGCCGG
CGAAGTCACATTGGAAAAATATGAGGCCAGAATAGCGGTAAAAGCAAAAGAATAG

Upstream 100 bases:

>100_bases
CATCTTTATCATGCTGCAAAAGAACTTTGTGTCAGGATTGACGGCAGGCGGAACGAAAGGATAGTCCGAAGGAGAAAATG
GTCTAAAAAAGGAGAGTATT

Downstream 100 bases:

>100_bases
GCCTGGGAGGACATCCGCTTTAAGGTGGATGTTTCTCCGGGTGAATAATGAAAGCGTTTTCTTTTTGCTTGTGTGTCTAT
TATGAAAGGGGAGAAGCGAG

Product: LacA

Products: NA

Alternate protein names: Beta-gal; Beta-1,4-galactooligomerase; Galactooligomerase [H]

Number of amino acids: Translated: 684; Mature: 683

Protein sequence:

>684_residues
MPKIYTTQARYMLHGGDYNPDQWLDRPDILADDIKLMKLAHTNTFSVGIFSWSALEPEEGVYTFEWLDDIFESIHRNGGR
IILATPSGARPAWLSQKYPEVLRVNAERVKQLHGGRHNHCFTSYVYREKTKEINRMLAERYGSQHALLMWHVSNEYGGEC
HCDQCQHAFRDWLKKKYNHDIKSLNDAWWTPFWSHTFNDWSQIESPSPIGENAVHGLNLDWRRFVTDQTISFFQNEIVPL
KEITPNIPITTNFMADTHDLIPFQGLDYSKFAKHLDVISWDAYPAWHNDWESTADLAMKVGFINDLYRSLKQQPFLLMES
TPSAVNWHDFNKAKRPGMHLLSSVQMIAHGSDSILYFQWRKSRGSSEKFHGAVVGHDNCSENRVFKEVAKVGQTLEALSE
VTGTIRPADVAILYDWENHWALQDAQGFGMKTKRYPQTLHEHYRAFWERDIPVDVITKEQDFSSYRLLIVPMLYLASEET
IARLKAFAANGGTLVMTYISGIVNESDLTYLGGWPKDLQEMFGMEPVETDTLYPGDKNAVRYQNRSYELKDYATVLKLST
ADPEGFYEDDFYADTTAVTSHPYKQGKTYYIGARLSSQFHRDFYGTLIKELAIQPALDVKHQPGVSVQVRQDEENDYIFI
MNFTEKRQPVVLASAVKDMLTGETLAGEVTLEKYEARIAVKAKE

Sequences:

>Translated_684_residues
MPKIYTTQARYMLHGGDYNPDQWLDRPDILADDIKLMKLAHTNTFSVGIFSWSALEPEEGVYTFEWLDDIFESIHRNGGR
IILATPSGARPAWLSQKYPEVLRVNAERVKQLHGGRHNHCFTSYVYREKTKEINRMLAERYGSQHALLMWHVSNEYGGEC
HCDQCQHAFRDWLKKKYNHDIKSLNDAWWTPFWSHTFNDWSQIESPSPIGENAVHGLNLDWRRFVTDQTISFFQNEIVPL
KEITPNIPITTNFMADTHDLIPFQGLDYSKFAKHLDVISWDAYPAWHNDWESTADLAMKVGFINDLYRSLKQQPFLLMES
TPSAVNWHDFNKAKRPGMHLLSSVQMIAHGSDSILYFQWRKSRGSSEKFHGAVVGHDNCSENRVFKEVAKVGQTLEALSE
VTGTIRPADVAILYDWENHWALQDAQGFGMKTKRYPQTLHEHYRAFWERDIPVDVITKEQDFSSYRLLIVPMLYLASEET
IARLKAFAANGGTLVMTYISGIVNESDLTYLGGWPKDLQEMFGMEPVETDTLYPGDKNAVRYQNRSYELKDYATVLKLST
ADPEGFYEDDFYADTTAVTSHPYKQGKTYYIGARLSSQFHRDFYGTLIKELAIQPALDVKHQPGVSVQVRQDEENDYIFI
MNFTEKRQPVVLASAVKDMLTGETLAGEVTLEKYEARIAVKAKE
>Mature_683_residues
PKIYTTQARYMLHGGDYNPDQWLDRPDILADDIKLMKLAHTNTFSVGIFSWSALEPEEGVYTFEWLDDIFESIHRNGGRI
ILATPSGARPAWLSQKYPEVLRVNAERVKQLHGGRHNHCFTSYVYREKTKEINRMLAERYGSQHALLMWHVSNEYGGECH
CDQCQHAFRDWLKKKYNHDIKSLNDAWWTPFWSHTFNDWSQIESPSPIGENAVHGLNLDWRRFVTDQTISFFQNEIVPLK
EITPNIPITTNFMADTHDLIPFQGLDYSKFAKHLDVISWDAYPAWHNDWESTADLAMKVGFINDLYRSLKQQPFLLMEST
PSAVNWHDFNKAKRPGMHLLSSVQMIAHGSDSILYFQWRKSRGSSEKFHGAVVGHDNCSENRVFKEVAKVGQTLEALSEV
TGTIRPADVAILYDWENHWALQDAQGFGMKTKRYPQTLHEHYRAFWERDIPVDVITKEQDFSSYRLLIVPMLYLASEETI
ARLKAFAANGGTLVMTYISGIVNESDLTYLGGWPKDLQEMFGMEPVETDTLYPGDKNAVRYQNRSYELKDYATVLKLSTA
DPEGFYEDDFYADTTAVTSHPYKQGKTYYIGARLSSQFHRDFYGTLIKELAIQPALDVKHQPGVSVQVRQDEENDYIFIM
NFTEKRQPVVLASAVKDMLTGETLAGEVTLEKYEARIAVKAKE

Specific function: Hydrolyzes oligosaccharides released by the endo-1,4- beta-galactosidase galA from arabinogalactan type I, a pectic plant polysaccharide. It is unable to use lactose as a sole carbon source [H]

COG id: COG1874

COG function: function code G; Beta-galactosidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 42 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013739
- InterPro:   IPR013738
- InterPro:   IPR013780
- InterPro:   IPR003476
- InterPro:   IPR013529
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF02449 Glyco_hydro_42; PF08533 Glyco_hydro_42C; PF08532 Glyco_hydro_42M [H]

EC number: =3.2.1.23 [H]

Molecular weight: Translated: 78852; Mature: 78720

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00018 EF_HAND_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKIYTTQARYMLHGGDYNPDQWLDRPDILADDIKLMKLAHTNTFSVGIFSWSALEPEEG
CCCEEECCEEEEEECCCCCHHHHCCCCCCHHHHHHHHEEECCCEEEEEEEEECCCCCCCC
VYTFEWLDDIFESIHRNGGRIILATPSGARPAWLSQKYPEVLRVNAERVKQLHGGRHNHC
EEEHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHCCHHHHHCCHHHHHHHHCCCCCCH
FTSYVYREKTKEINRMLAERYGSQHALLMWHVSNEYGGECHCDQCQHAFRDWLKKKYNHD
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCH
IKSLNDAWWTPFWSHTFNDWSQIESPSPIGENAVHGLNLDWRRFVTDQTISFFQNEIVPL
HHHCCHHHCCCCHHCCCCCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCH
KEITPNIPITTNFMADTHDLIPFQGLDYSKFAKHLDVISWDAYPAWHNDWESTADLAMKV
HHCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHCEEECCCCCCCCCCCHHHHHHHHHH
GFINDLYRSLKQQPFLLMESTPSAVNWHDFNKAKRPGMHLLSSVQMIAHGSDSILYFQWR
HHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEE
KSRGSSEKFHGAVVGHDNCSENRVFKEVAKVGQTLEALSEVTGTIRPADVAILYDWENHW
CCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCE
ALQDAQGFGMKTKRYPQTLHEHYRAFWERDIPVDVITKEQDFSSYRLLIVPMLYLASEET
EEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHCCHHH
IARLKAFAANGGTLVMTYISGIVNESDLTYLGGWPKDLQEMFGMEPVETDTLYPGDKNAV
HHHHHHHHCCCCEEEEEHHHHHCCCCCCEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCE
RYQNRSYELKDYATVLKLSTADPEGFYEDDFYADTTAVTSHPYKQGKTYYIGARLSSQFH
EECCCCCCHHHHEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHCCCEEEEEEHHHHHHH
RDFYGTLIKELAIQPALDVKHQPGVSVQVRQDEENDYIFIMNFTEKRQPVVLASAVKDML
HHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHH
TGETLAGEVTLEKYEARIAVKAKE
CCCCCCCCEEEEEEEEEEEEEECC
>Mature Secondary Structure 
PKIYTTQARYMLHGGDYNPDQWLDRPDILADDIKLMKLAHTNTFSVGIFSWSALEPEEG
CCEEECCEEEEEECCCCCHHHHCCCCCCHHHHHHHHEEECCCEEEEEEEEECCCCCCCC
VYTFEWLDDIFESIHRNGGRIILATPSGARPAWLSQKYPEVLRVNAERVKQLHGGRHNHC
EEEHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHCCHHHHHCCHHHHHHHHCCCCCCH
FTSYVYREKTKEINRMLAERYGSQHALLMWHVSNEYGGECHCDQCQHAFRDWLKKKYNHD
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCH
IKSLNDAWWTPFWSHTFNDWSQIESPSPIGENAVHGLNLDWRRFVTDQTISFFQNEIVPL
HHHCCHHHCCCCHHCCCCCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCH
KEITPNIPITTNFMADTHDLIPFQGLDYSKFAKHLDVISWDAYPAWHNDWESTADLAMKV
HHCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHCEEECCCCCCCCCCCHHHHHHHHHH
GFINDLYRSLKQQPFLLMESTPSAVNWHDFNKAKRPGMHLLSSVQMIAHGSDSILYFQWR
HHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEE
KSRGSSEKFHGAVVGHDNCSENRVFKEVAKVGQTLEALSEVTGTIRPADVAILYDWENHW
CCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCE
ALQDAQGFGMKTKRYPQTLHEHYRAFWERDIPVDVITKEQDFSSYRLLIVPMLYLASEET
EEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHCCHHH
IARLKAFAANGGTLVMTYISGIVNESDLTYLGGWPKDLQEMFGMEPVETDTLYPGDKNAV
HHHHHHHHCCCCEEEEEHHHHHCCCCCCEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCE
RYQNRSYELKDYATVLKLSTADPEGFYEDDFYADTTAVTSHPYKQGKTYYIGARLSSQFH
EECCCCCCHHHHEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHCCCEEEEEEHHHHHHH
RDFYGTLIKELAIQPALDVKHQPGVSVQVRQDEENDYIFIMNFTEKRQPVVLASAVKDML
HHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHH
TGETLAGEVTLEKYEARIAVKAKE
CCCCCCCCEEEEEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA