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The map label for this gene is iolB

Identifier: 52787927

GI number: 52787927

Start: 4088552

End: 4089367

Strand: Reverse

Name: iolB

Synonym: BLi04250

Alternate gene names: 52787927

Gene position: 4089367-4088552 (Counterclockwise)

Preceding gene: 52787928

Following gene: 52787926

Centisome position: 96.84

GC content: 47.43

Gene sequence:

>816_bases
ATGAGTCATTTGTTGCGCAAGCCGCAGGCGAATGAGCTGTCACAAGGCGTGAAGCTTGTACACGAAGTGAAAAAATCGAA
TTCCGATCTAAGTTATGTCGAGTTTAAAGTCCTTGATCTTGCACCGGGATCCAGCTATGAAGAATCGCTGAGCAAACAAG
AATGCTGCATCGTCGCGCTTACCGGAAAGATTACCGCAACCGATCATGAACAGACATTTGAAAATATCGGCACGAGAGAA
AGCGTTTTCGAAAGAAAGCCGACCGACAGCGTATATGTGTCAAATGACCGCAAGTTCGGCATTACCGCCGTTACAGAGGC
AAGAGTTGCCCTGTGCTATTCACCTTCTGAAAACCAGCTTCCGACGAAACTGATCAAAGCTGAAGACAACGGTATTGAGA
ACCGGGGAAAATTCAGCAATAAACGAACCGTCCATAACATACTTCCGGATTCAGATCCTTCAGCGAACAGTCTTTTAGTC
GTCGAAGTCTACACTGAAAGCGGAAACTGGTCCAGCTATCCGCCGCACAAGCATGACCAAGACAACCTGCCTGAAGAGTC
CTTTTTAGAAGAAACATACTACCATGAACTTGATCCGCAGCAAGGCTTCGTATTTCAGCGCGTATACACGGATGACCGTT
CCATCGACGAGACGATGACTGTTGAAAATGGAAACGTTGTCATCGTGCCGGCCGGATACCATCCTGTCGGGGTGCCTGAC
GGATACACATCCTATTATTTAAACGTGATGGCAGGGCCGACGCGTAAATGGAAGTTTCATAACGACCCTGACCATGAATG
GATTTTGGAACGCTAA

Upstream 100 bases:

>100_bases
GATATCCGGCGCCTGACTTCAATTAATTGTGAAAAAACCGGGCGGTGCGGTTCCCGCGCTGTTCGGATCATGTTTCAAGT
TTGAAAAGGAGGCTTTATCC

Downstream 100 bases:

>100_bases
CAATAAGGCAAGGAGAGGCTGTTGAAGATGAAGTATACGTTTAATGAAGAGAAAGAATTTGACATTGTAGCGATCGGCCG
CGCATGCATCGATTTAAACG

Product: IolB

Products: NA

Alternate protein names: 5DG isomerase

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MSHLLRKPQANELSQGVKLVHEVKKSNSDLSYVEFKVLDLAPGSSYEESLSKQECCIVALTGKITATDHEQTFENIGTRE
SVFERKPTDSVYVSNDRKFGITAVTEARVALCYSPSENQLPTKLIKAEDNGIENRGKFSNKRTVHNILPDSDPSANSLLV
VEVYTESGNWSSYPPHKHDQDNLPEESFLEETYYHELDPQQGFVFQRVYTDDRSIDETMTVENGNVVIVPAGYHPVGVPD
GYTSYYLNVMAGPTRKWKFHNDPDHEWILER

Sequences:

>Translated_271_residues
MSHLLRKPQANELSQGVKLVHEVKKSNSDLSYVEFKVLDLAPGSSYEESLSKQECCIVALTGKITATDHEQTFENIGTRE
SVFERKPTDSVYVSNDRKFGITAVTEARVALCYSPSENQLPTKLIKAEDNGIENRGKFSNKRTVHNILPDSDPSANSLLV
VEVYTESGNWSSYPPHKHDQDNLPEESFLEETYYHELDPQQGFVFQRVYTDDRSIDETMTVENGNVVIVPAGYHPVGVPD
GYTSYYLNVMAGPTRKWKFHNDPDHEWILER
>Mature_270_residues
SHLLRKPQANELSQGVKLVHEVKKSNSDLSYVEFKVLDLAPGSSYEESLSKQECCIVALTGKITATDHEQTFENIGTRES
VFERKPTDSVYVSNDRKFGITAVTEARVALCYSPSENQLPTKLIKAEDNGIENRGKFSNKRTVHNILPDSDPSANSLLVV
EVYTESGNWSSYPPHKHDQDNLPEESFLEETYYHELDPQQGFVFQRVYTDDRSIDETMTVENGNVVIVPAGYHPVGVPDG
YTSYYLNVMAGPTRKWKFHNDPDHEWILER

Specific function: Involved in the isomerization of 5-deoxy-glucuronate (5DG) to 5-dehydro-2-deoxy-D-gluconate (DKG or 2-deoxy-5-keto-D- gluconate)

COG id: COG3718

COG function: function code G; Uncharacterized enzyme involved in inositol metabolism

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isomerase iolB family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): IOLB_BACLD (Q65D01)

Other databases:

- EMBL:   CP000002
- EMBL:   AE017333
- RefSeq:   YP_081322.1
- RefSeq:   YP_093756.1
- ProteinModelPortal:   Q65D01
- SMR:   Q65D01
- STRING:   Q65D01
- EnsemblBacteria:   EBBACT00000056830
- EnsemblBacteria:   EBBACT00000062140
- GeneID:   3030838
- GeneID:   3098182
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi04250
- KEGG:   bli:BL00245
- NMPDR:   fig|279010.5.peg.4029
- eggNOG:   COG3718
- GeneTree:   EBGT00050000000899
- HOGENOM:   HBG465614
- OMA:   NIMPEDD
- ProtClustDB:   CLSK873290
- BioCyc:   BLIC279010-1:BLI04250-MONOMER
- BioCyc:   BLIC279010:BL00245-MONOMER
- HAMAP:   MF_01673
- InterPro:   IPR011051
- InterPro:   IPR021120
- InterPro:   IPR014710
- Gene3D:   G3DSA:2.60.120.10

Pfam domain/function: PF04962 KduI; SSF51182 RmlC_like_cupin

EC number: NA

Molecular weight: Translated: 30746; Mature: 30615

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHLLRKPQANELSQGVKLVHEVKKSNSDLSYVEFKVLDLAPGSSYEESLSKQECCIVAL
CCCHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCHHHHHCCCCEEEEEE
TGKITATDHEQTFENIGTRESVFERKPTDSVYVSNDRKFGITAVTEARVALCYSPSENQL
ECEEEECCHHHHHHHCCCHHHHHHCCCCCCEEEECCCEECEEEEECEEEEEEECCCCCCC
PTKLIKAEDNGIENRGKFSNKRTVHNILPDSDPSANSLLVVEVYTESGNWSSYPPHKHDQ
CHHEEECCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCC
DNLPEESFLEETYYHELDPQQGFVFQRVYTDDRSIDETMTVENGNVVIVPAGYHPVGVPD
CCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEEEECCCEEEEECCCCCCCCCC
GYTSYYLNVMAGPTRKWKFHNDPDHEWILER
CCHHEEEEEEECCCCCCCCCCCCCCCEECCC
>Mature Secondary Structure 
SHLLRKPQANELSQGVKLVHEVKKSNSDLSYVEFKVLDLAPGSSYEESLSKQECCIVAL
CCHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCHHHHHCCCCEEEEEE
TGKITATDHEQTFENIGTRESVFERKPTDSVYVSNDRKFGITAVTEARVALCYSPSENQL
ECEEEECCHHHHHHHCCCHHHHHHCCCCCCEEEECCCEECEEEEECEEEEEEECCCCCCC
PTKLIKAEDNGIENRGKFSNKRTVHNILPDSDPSANSLLVVEVYTESGNWSSYPPHKHDQ
CHHEEECCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCC
DNLPEESFLEETYYHELDPQQGFVFQRVYTDDRSIDETMTVENGNVVIVPAGYHPVGVPD
CCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEEEECCCEEEEECCCCCCCCCC
GYTSYYLNVMAGPTRKWKFHNDPDHEWILER
CCHHEEEEEEECCCCCCCCCCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA