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The map label for this gene is rhgT [H]

Identifier: 52787777

GI number: 52787777

Start: 3925103

End: 3925765

Strand: Reverse

Name: rhgT [H]

Synonym: BLi04100

Alternate gene names: 52787777

Gene position: 3925765-3925103 (Counterclockwise)

Preceding gene: 52787780

Following gene: 52787776

Centisome position: 92.97

GC content: 48.11

Gene sequence:

>663_bases
ATGATGCGAGACATTCAGTTGTTTTTGGCCGGGGACTCAACGGTTTGCGATTATGATGAGAATAGGGCGCCGCGTGCAGG
CTGGGGGCAGAAAATCGGCGAGTTTTTAAGCGCAGGTGTTTTGATCCGCAATGAAGCGGCATCAGGAAGAAGCTCAAAAA
GCTTTATTGAAGAAGGCAGGCTGAAGCGAATCATAACACAGATCAAAAGCGGCGATTATTTGTTTGTCCAGTTCGGTCAT
AACGACCAAAAACCGGATGATCGCTATACAGAGCCATACACAACTTACAAGCAGCATTTGACCCGGTATATAGATGAGGC
ACGGTTAAAGAAAGCCAATCCTGTTCTGATTACACCGGTCCAGAGAAGATCATTTGCAAGCGATGGCCGCTTTAAAAATA
CCCATGGCGACTATCCTGATGCGATGATACAGCTTGCGGAAGAGCTTCGGGTGCCGCTTATCGATCTCGCCTTCAAAAGC
CGTCAATTGCTTGAATCGCTAGGACCGGAAGCTTCCAAAAAGCTGTATTTATGGCTTGAACCGGGGGAACACCCCAACTA
TCCGGACGGAGCCAGGGATGACACCCACTTTTGCGACTTCGGGGCAAAAGAAATCGCTAAGCTTGTTGCACAGGAAGCAA
AAGAAGCGGGAATTCCTTTATAA

Upstream 100 bases:

>100_bases
AAAAAGGAGTCGTATAAAGAACAAAATGGTTAATTATGTATATTTGTTGCAGTTCCTTGAGGGGCGTTTTAATATAAATA
AAAAGGGATCAGGGGGATGG

Downstream 100 bases:

>100_bases
AACAGCCTACTTGAGAATAATTTTCAATTATTTTTCGAAATTTTTGCACTTCCGATGCCAAACTGTGATACGATCAATCT
CGTAAAGATTTTCAAAAGGA

Product: hypothetical protein

Products: NA

Alternate protein names: RGAE [H]

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MMRDIQLFLAGDSTVCDYDENRAPRAGWGQKIGEFLSAGVLIRNEAASGRSSKSFIEEGRLKRIITQIKSGDYLFVQFGH
NDQKPDDRYTEPYTTYKQHLTRYIDEARLKKANPVLITPVQRRSFASDGRFKNTHGDYPDAMIQLAEELRVPLIDLAFKS
RQLLESLGPEASKKLYLWLEPGEHPNYPDGARDDTHFCDFGAKEIAKLVAQEAKEAGIPL

Sequences:

>Translated_220_residues
MMRDIQLFLAGDSTVCDYDENRAPRAGWGQKIGEFLSAGVLIRNEAASGRSSKSFIEEGRLKRIITQIKSGDYLFVQFGH
NDQKPDDRYTEPYTTYKQHLTRYIDEARLKKANPVLITPVQRRSFASDGRFKNTHGDYPDAMIQLAEELRVPLIDLAFKS
RQLLESLGPEASKKLYLWLEPGEHPNYPDGARDDTHFCDFGAKEIAKLVAQEAKEAGIPL
>Mature_220_residues
MMRDIQLFLAGDSTVCDYDENRAPRAGWGQKIGEFLSAGVLIRNEAASGRSSKSFIEEGRLKRIITQIKSGDYLFVQFGH
NDQKPDDRYTEPYTTYKQHLTRYIDEARLKKANPVLITPVQRRSFASDGRFKNTHGDYPDAMIQLAEELRVPLIDLAFKS
RQLLESLGPEASKKLYLWLEPGEHPNYPDGARDDTHFCDFGAKEIAKLVAQEAKEAGIPL

Specific function: May play role in the degradation of type I rhamnogalacturonan derived from plant cell walls. This enzyme has a broad substrate specificity, and shows strong preference for glucose pentaacetate, beta-naphthylacetate, and p-nitrophenyl acetate (pNPA). Also

COG id: COG2755

COG function: function code E; Lysophospholipase L1 and related esterases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDSL' lipolytic enzyme family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013830
- InterPro:   IPR013831
- InterPro:   IPR001087 [H]

Pfam domain/function: PF00657 Lipase_GDSL [H]

EC number: NA

Molecular weight: Translated: 24951; Mature: 24951

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRDIQLFLAGDSTVCDYDENRAPRAGWGQKIGEFLSAGVLIRNEAASGRSSKSFIEEGR
CCCEEEEEEECCCEEECCCCCCCCCCCHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHH
LKRIITQIKSGDYLFVQFGHNDQKPDDRYTEPYTTYKQHLTRYIDEARLKKANPVLITPV
HHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECH
QRRSFASDGRFKNTHGDYPDAMIQLAEELRVPLIDLAFKSRQLLESLGPEASKKLYLWLE
HHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEC
PGEHPNYPDGARDDTHFCDFGAKEIAKLVAQEAKEAGIPL
CCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MMRDIQLFLAGDSTVCDYDENRAPRAGWGQKIGEFLSAGVLIRNEAASGRSSKSFIEEGR
CCCEEEEEEECCCEEECCCCCCCCCCCHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHH
LKRIITQIKSGDYLFVQFGHNDQKPDDRYTEPYTTYKQHLTRYIDEARLKKANPVLITPV
HHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECH
QRRSFASDGRFKNTHGDYPDAMIQLAEELRVPLIDLAFKSRQLLESLGPEASKKLYLWLE
HHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEC
PGEHPNYPDGARDDTHFCDFGAKEIAKLVAQEAKEAGIPL
CCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]