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The map label for this gene is alsS [H]

Identifier: 52787527

GI number: 52787527

Start: 3675142

End: 3676860

Strand: Reverse

Name: alsS [H]

Synonym: BLi03848

Alternate gene names: 52787527

Gene position: 3676860-3675142 (Counterclockwise)

Preceding gene: 52787529

Following gene: 52787526

Centisome position: 87.07

GC content: 50.15

Gene sequence:

>1719_bases
TTGAATAATGTAGCCGCTAAAAATGAAACTCTTACTGTAAGAGGAGCAGAGCTTGTGGTGGATAGTCTCATTCAGCAAGG
TGTCACTCATGTTTTCGGTATTCCGGGAGCGAAAATCGATGCGGTGTTTGACGTATTGAAAGACAAGGGGCCTGAATTGA
TCGTTTGCCGTCACGAGCAGAATGCAGCATTTATGGCGGCGGCAGTCGGACGATTGACTGGAAAGCCCGGTGTTTGCCTG
GTGACTTCAGGTCCGGGAGCGTCTAATTTAGCGACCGGTCTTGTAACAGCCAATACAGAAGGAGATCCGGTTGTTGCCCT
GGCGGGTGCTGTAAAAAGAGCGGATCGTCTCAAAAAAACTCATCAATCGATGGATAATGCGGCGTTGTTTCAGCCGATTA
CGAAATATAGCGCAGAAGTGGAAGATGCGAACAACATACCTGAGGCTGTAACCAATGCATTCAGAGCGGCGGCTTCTGGA
CAGGCTGGCGCAGCGTTTCTCAGCTTTCCGCAGGACGTTACGGCCGGTCCGGCAACTGCCAAGCCGGTGAAAACCATGCC
GGCGCCGAAGCTGGGCGCGGCTTCGGACGAACAAATCAGCGCGGCCATCGCCAAAATTCACAATGCGAACCTTCCTGTCG
TGCTTGTCGGGATGAAAGGCGGAAGACCTGAAGCGATTGAAGCGGTTCGGCGTCTGCTAAGGAAAGTGAAACTGCCGTTT
GTTGAAACATACCAAGCAGCGGGTACGCTGTCTCACGATTTGGAAGACCAGTACTTCGGCCGGATCGGACTATTCCGCAA
TCAGCCCGGAGACATGCTATTGGAAAAAGCGGATGTCGTTTTGACGGTCGGCTATGATCCGATTGAATACGATCCGGTCT
TTTGGAATGGAAAAGGCGAACGAAGCGTGATTCATCTTGACGAAATACAAGCCGATATCGATCATGACTATCAGCCCGAA
ATCGAGTTGATCGGCGATATCGCAGAGACGTTAAACCATATTGAACATGATTCTCTGCCGGTTTCCATCGACGAGTCCTT
TGCACCTGTCCTTGATTACTTGAAGAAAGCACTGGAGGAGCAAAGCGAACCTCCTAAAGAAACAAAAACTGATCTTGTTC
ATCCGCTGCAAATCGTTCGCGATTTGCGTGAACTGCTGAGCGATGACATAACGGTGACTTGCGACATCGGCTCCCATGCG
ATTTGGATGTCTAGATATTTCCGCACCTACCGTCCGCATGGACTGCTGATTTCCAACGGCATGCAGACGCTTGGGGTGGC
TTTGCCGTGGGCGATTGCAGCAACGCTGGTCAATCCGGGACAGAAAGTCGTGTCTGTTTCCGGGGACGGGGGCTTCCTGT
TCTCCGCGATGGAGCTTGAGACAGCGGTCAGATTAAAAGCGCCGATCGTTCACATTGTCTGGAATGACAGCACATACGAT
ATGGTTGCGTTCCAGCAGGAGATGAAATACAAGCGGACGTCCGGAGTCGACTTCGGCGGAATCGATATTGTAAAATATGC
TGAAAGCTTTGGTGCAAAAGGTTTAAGGGTCAATTCACCTGACGAATTGGCAGAGGTGCTTAAAGCTGGTCTTGATGCAG
AGGGGCCTGTTGTCATTGATATTCCGGTCGATTACAGCGACAACATTCATTTGGCCGATCAGCGTTTTCCAAAAAAATTT
GAGGAACATTTTAATAAAGAAGCCTCTAAGCAATCTTGA

Upstream 100 bases:

>100_bases
AGAAGTATTGGAAAACAATTTCCACAAGATGTATATTTAATAATACAATAATTTTATTAAAAATTCATTGTAAATGAATG
AAAATGGAGGAGTGAGGGCT

Downstream 100 bases:

>100_bases
TCACAGCATGTCCAGTCACAACGAACAAAATCAAGGGAGTGAAAAGAGGAAAATGAAAAGTGCAAGCAAACAAAAAATAA
TTCAGCCCGTTGATAAGAAC

Product: acetolactate synthase

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase [H]

Number of amino acids: Translated: 572; Mature: 572

Protein sequence:

>572_residues
MNNVAAKNETLTVRGAELVVDSLIQQGVTHVFGIPGAKIDAVFDVLKDKGPELIVCRHEQNAAFMAAAVGRLTGKPGVCL
VTSGPGASNLATGLVTANTEGDPVVALAGAVKRADRLKKTHQSMDNAALFQPITKYSAEVEDANNIPEAVTNAFRAAASG
QAGAAFLSFPQDVTAGPATAKPVKTMPAPKLGAASDEQISAAIAKIHNANLPVVLVGMKGGRPEAIEAVRRLLRKVKLPF
VETYQAAGTLSHDLEDQYFGRIGLFRNQPGDMLLEKADVVLTVGYDPIEYDPVFWNGKGERSVIHLDEIQADIDHDYQPE
IELIGDIAETLNHIEHDSLPVSIDESFAPVLDYLKKALEEQSEPPKETKTDLVHPLQIVRDLRELLSDDITVTCDIGSHA
IWMSRYFRTYRPHGLLISNGMQTLGVALPWAIAATLVNPGQKVVSVSGDGGFLFSAMELETAVRLKAPIVHIVWNDSTYD
MVAFQQEMKYKRTSGVDFGGIDIVKYAESFGAKGLRVNSPDELAEVLKAGLDAEGPVVIDIPVDYSDNIHLADQRFPKKF
EEHFNKEASKQS

Sequences:

>Translated_572_residues
MNNVAAKNETLTVRGAELVVDSLIQQGVTHVFGIPGAKIDAVFDVLKDKGPELIVCRHEQNAAFMAAAVGRLTGKPGVCL
VTSGPGASNLATGLVTANTEGDPVVALAGAVKRADRLKKTHQSMDNAALFQPITKYSAEVEDANNIPEAVTNAFRAAASG
QAGAAFLSFPQDVTAGPATAKPVKTMPAPKLGAASDEQISAAIAKIHNANLPVVLVGMKGGRPEAIEAVRRLLRKVKLPF
VETYQAAGTLSHDLEDQYFGRIGLFRNQPGDMLLEKADVVLTVGYDPIEYDPVFWNGKGERSVIHLDEIQADIDHDYQPE
IELIGDIAETLNHIEHDSLPVSIDESFAPVLDYLKKALEEQSEPPKETKTDLVHPLQIVRDLRELLSDDITVTCDIGSHA
IWMSRYFRTYRPHGLLISNGMQTLGVALPWAIAATLVNPGQKVVSVSGDGGFLFSAMELETAVRLKAPIVHIVWNDSTYD
MVAFQQEMKYKRTSGVDFGGIDIVKYAESFGAKGLRVNSPDELAEVLKAGLDAEGPVVIDIPVDYSDNIHLADQRFPKKF
EEHFNKEASKQS
>Mature_572_residues
MNNVAAKNETLTVRGAELVVDSLIQQGVTHVFGIPGAKIDAVFDVLKDKGPELIVCRHEQNAAFMAAAVGRLTGKPGVCL
VTSGPGASNLATGLVTANTEGDPVVALAGAVKRADRLKKTHQSMDNAALFQPITKYSAEVEDANNIPEAVTNAFRAAASG
QAGAAFLSFPQDVTAGPATAKPVKTMPAPKLGAASDEQISAAIAKIHNANLPVVLVGMKGGRPEAIEAVRRLLRKVKLPF
VETYQAAGTLSHDLEDQYFGRIGLFRNQPGDMLLEKADVVLTVGYDPIEYDPVFWNGKGERSVIHLDEIQADIDHDYQPE
IELIGDIAETLNHIEHDSLPVSIDESFAPVLDYLKKALEEQSEPPKETKTDLVHPLQIVRDLRELLSDDITVTCDIGSHA
IWMSRYFRTYRPHGLLISNGMQTLGVALPWAIAATLVNPGQKVVSVSGDGGFLFSAMELETAVRLKAPIVHIVWNDSTYD
MVAFQQEMKYKRTSGVDFGGIDIVKYAESFGAKGLRVNSPDELAEVLKAGLDAEGPVVIDIPVDYSDNIHLADQRFPKKF
EEHFNKEASKQS

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=574, Percent_Identity=26.3066202090592, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI21361361, Length=602, Percent_Identity=24.5847176079734, Blast_Score=135, Evalue=1e-31,
Organism=Escherichia coli, GI87081685, Length=556, Percent_Identity=28.5971223021583, Blast_Score=218, Evalue=1e-57,
Organism=Escherichia coli, GI1790104, Length=550, Percent_Identity=29.2727272727273, Blast_Score=217, Evalue=2e-57,
Organism=Escherichia coli, GI1786717, Length=565, Percent_Identity=24.6017699115044, Blast_Score=162, Evalue=6e-41,
Organism=Escherichia coli, GI1787096, Length=540, Percent_Identity=25.9259259259259, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1788716, Length=561, Percent_Identity=26.0249554367201, Blast_Score=148, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI17531299, Length=553, Percent_Identity=28.0289330922242, Blast_Score=145, Evalue=8e-35,
Organism=Caenorhabditis elegans, GI17531301, Length=553, Percent_Identity=27.8481012658228, Blast_Score=145, Evalue=8e-35,
Organism=Caenorhabditis elegans, GI17542570, Length=612, Percent_Identity=24.6732026143791, Blast_Score=139, Evalue=5e-33,
Organism=Saccharomyces cerevisiae, GI6323755, Length=564, Percent_Identity=28.9007092198582, Blast_Score=210, Evalue=6e-55,
Organism=Saccharomyces cerevisiae, GI6320816, Length=487, Percent_Identity=25.8726899383984, Blast_Score=128, Evalue=3e-30,
Organism=Drosophila melanogaster, GI19922626, Length=582, Percent_Identity=28.5223367697594, Blast_Score=173, Evalue=4e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012782
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 61932; Mature: 61932

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNVAAKNETLTVRGAELVVDSLIQQGVTHVFGIPGAKIDAVFDVLKDKGPELIVCRHEQ
CCCCCCCCCEEEEEHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHCCCCCEEEEECCC
NAAFMAAAVGRLTGKPGVCLVTSGPGASNLATGLVTANTEGDPVVALAGAVKRADRLKKT
CCHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEEECCCCCCEEEHHHHHHHHHHHHHH
HQSMDNAALFQPITKYSAEVEDANNIPEAVTNAFRAAASGQAGAAFLSFPQDVTAGPATA
HHHHCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCC
KPVKTMPAPKLGAASDEQISAAIAKIHNANLPVVLVGMKGGRPEAIEAVRRLLRKVKLPF
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCH
VETYQAAGTLSHDLEDQYFGRIGLFRNQPGDMLLEKADVVLTVGYDPIEYDPVFWNGKGE
HHHHHHHCCHHHCCCHHHHCCCCCCCCCCHHHHEECCCEEEEECCCCCCCCCEEECCCCC
RSVIHLDEIQADIDHDYQPEIELIGDIAETLNHIEHDSLPVSIDESFAPVLDYLKKALEE
CEEEEHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHH
QSEPPKETKTDLVHPLQIVRDLRELLSDDITVTCDIGSHAIWMSRYFRTYRPHGLLISNG
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEECC
MQTLGVALPWAIAATLVNPGQKVVSVSGDGGFLFSAMELETAVRLKAPIVHIVWNDSTYD
HHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEHHHHHHHHEEECCEEEEEECCCCHH
MVAFQQEMKYKRTSGVDFGGIDIVKYAESFGAKGLRVNSPDELAEVLKAGLDAEGPVVID
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCEEEE
IPVDYSDNIHLADQRFPKKFEEHFNKEASKQS
EECCCCCCEEECHHHHHHHHHHHHCHHCCCCC
>Mature Secondary Structure
MNNVAAKNETLTVRGAELVVDSLIQQGVTHVFGIPGAKIDAVFDVLKDKGPELIVCRHEQ
CCCCCCCCCEEEEEHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHCCCCCEEEEECCC
NAAFMAAAVGRLTGKPGVCLVTSGPGASNLATGLVTANTEGDPVVALAGAVKRADRLKKT
CCHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEEECCCCCCEEEHHHHHHHHHHHHHH
HQSMDNAALFQPITKYSAEVEDANNIPEAVTNAFRAAASGQAGAAFLSFPQDVTAGPATA
HHHHCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCC
KPVKTMPAPKLGAASDEQISAAIAKIHNANLPVVLVGMKGGRPEAIEAVRRLLRKVKLPF
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCH
VETYQAAGTLSHDLEDQYFGRIGLFRNQPGDMLLEKADVVLTVGYDPIEYDPVFWNGKGE
HHHHHHHCCHHHCCCHHHHCCCCCCCCCCHHHHEECCCEEEEECCCCCCCCCEEECCCCC
RSVIHLDEIQADIDHDYQPEIELIGDIAETLNHIEHDSLPVSIDESFAPVLDYLKKALEE
CEEEEHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHH
QSEPPKETKTDLVHPLQIVRDLRELLSDDITVTCDIGSHAIWMSRYFRTYRPHGLLISNG
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEECC
MQTLGVALPWAIAATLVNPGQKVVSVSGDGGFLFSAMELETAVRLKAPIVHIVWNDSTYD
HHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEHHHHHHHHEEECCEEEEEECCCCHH
MVAFQQEMKYKRTSGVDFGGIDIVKYAESFGAKGLRVNSPDELAEVLKAGLDAEGPVVID
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCEEEE
IPVDYSDNIHLADQRFPKKFEEHFNKEASKQS
EECCCCCCEEECHHHHHHHHHHHHCHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7685336; 9353933; 9384377; 10809684 [H]