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The map label for this gene is prfB [H]

Identifier: 52787452

GI number: 52787452

Start: 3593209

End: 3594192

Strand: Reverse

Name: prfB [H]

Synonym: BLi03772

Alternate gene names: 52787452

Gene position: 3594192-3593209 (Counterclockwise)

Preceding gene: 52787453

Following gene: 52787451

Centisome position: 85.12

GC content: 48.68

Gene sequence:

>984_bases
ATGGCTGACCCCGACTTTTGGGGAGATCAGCAGAAAGCGCAGACGATCATTAATGAAGCGAATGCGTTAAAAGACTATGT
CAACACGTACAAAAGTTTAAGCGAGTCTCATGAAGAACTGCAAATGACACATGATCTTTTAAAAGAAGATCCTGACCAGG
ACCTGCAAAATGAACTGGTCAACGAGCTGAAAAGCTTAACAAAGCAGTTTAATGAATTTGAGCTCCAGCTATTGTTAAGC
GAGCCTTATGATAAAAACAATGCGATCCTTGAGCTTCACCCGGGAGCCGGCGGCACCGAATCACAGGATTGGGGTTCAAT
GCTTCTCAGAATGTACACCAGGTGGGCAGAGCGCCGCGGATTCAAGGTGGAAACCCTTGATTACCTTCCAGGAGATGAGG
CGGGAATCAAGTCTGTCACTCTCCTGATTAAAGGCCACAATGCCTACGGTTATTTAAAAGCCGAAAAAGGCGTGCACAGG
CTCGTCAGAATTTCTCCGTTTGACTCTTCAGGGCGCAGGCACACGTCGTTTGTGTCTTGTGATGTCATGCCTGAATTCAA
CGAAGAAATCGACATTGAGATCCGTACGGAAGATATTAAAGTCGATACGTACAGGGCAAGCGGCGCCGGCGGCCAGCACG
TCAATACGACAGACTCCGCCGTCAGGATCACCCACTTGCCGACAGGCGTCATCGTCACTTGTCAGACAGAGCGATCACAA
ATTAAAAACCGCGAACGTGCCATGAAAATGCTGAAATCGAAGCTGTATCAGCGCAGAATCGAGGAGCAGCAGGCACAGCT
GGACGAAATTCGCGGCGAACAAAAAGAAATCGGCTGGGGAAGCCAGATCCGCTCATACGTTTTCCACCCGTATTCTCTAG
TGAAGGATCATCGAACCAACACGGAAATGGGGAACGTGCAGGCGGTTATGGACGGAGACATTGACTCTTTCATTGACGCA
TATTTGCGTTCCAAGCTTTCCTAA

Upstream 100 bases:

>100_bases
AGACAGGAACTTGAAAATATGGCTACTCGGTTAGCGGACTTCAGGGGGTCTCTTTGACCTCGAAACAAAGGAAGCAAGAA
TCAGCGAATTAGACGAGCAG

Downstream 100 bases:

>100_bases
ATTGAAGAAAGGCGCATCCACATTGTGAGGATGTGCCTTTTTTGCGGGGAAAATTGCCTGAATCTGGGCAGCTTAAGCCG
TAAGAAGCTTTGTCCCTCTG

Product: hypothetical protein

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MADPDFWGDQQKAQTIINEANALKDYVNTYKSLSESHEELQMTHDLLKEDPDQDLQNELVNELKSLTKQFNEFELQLLLS
EPYDKNNAILELHPGAGGTESQDWGSMLLRMYTRWAERRGFKVETLDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHR
LVRISPFDSSGRRHTSFVSCDVMPEFNEEIDIEIRTEDIKVDTYRASGAGGQHVNTTDSAVRITHLPTGVIVTCQTERSQ
IKNRERAMKMLKSKLYQRRIEEQQAQLDEIRGEQKEIGWGSQIRSYVFHPYSLVKDHRTNTEMGNVQAVMDGDIDSFIDA
YLRSKLS

Sequences:

>Translated_327_residues
MADPDFWGDQQKAQTIINEANALKDYVNTYKSLSESHEELQMTHDLLKEDPDQDLQNELVNELKSLTKQFNEFELQLLLS
EPYDKNNAILELHPGAGGTESQDWGSMLLRMYTRWAERRGFKVETLDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHR
LVRISPFDSSGRRHTSFVSCDVMPEFNEEIDIEIRTEDIKVDTYRASGAGGQHVNTTDSAVRITHLPTGVIVTCQTERSQ
IKNRERAMKMLKSKLYQRRIEEQQAQLDEIRGEQKEIGWGSQIRSYVFHPYSLVKDHRTNTEMGNVQAVMDGDIDSFIDA
YLRSKLS
>Mature_326_residues
ADPDFWGDQQKAQTIINEANALKDYVNTYKSLSESHEELQMTHDLLKEDPDQDLQNELVNELKSLTKQFNEFELQLLLSE
PYDKNNAILELHPGAGGTESQDWGSMLLRMYTRWAERRGFKVETLDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHRL
VRISPFDSSGRRHTSFVSCDVMPEFNEEIDIEIRTEDIKVDTYRASGAGGQHVNTTDSAVRITHLPTGVIVTCQTERSQI
KNRERAMKMLKSKLYQRRIEEQQAQLDEIRGEQKEIGWGSQIRSYVFHPYSLVKDHRTNTEMGNVQAVMDGDIDSFIDAY
LRSKLS

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=294, Percent_Identity=40.1360544217687, Blast_Score=205, Evalue=5e-53,
Organism=Homo sapiens, GI34577120, Length=302, Percent_Identity=37.7483443708609, Blast_Score=178, Evalue=7e-45,
Organism=Homo sapiens, GI166795305, Length=208, Percent_Identity=39.9038461538462, Blast_Score=147, Evalue=1e-35,
Organism=Escherichia coli, GI2367172, Length=323, Percent_Identity=48.9164086687306, Blast_Score=327, Evalue=9e-91,
Organism=Escherichia coli, GI1787462, Length=326, Percent_Identity=35.2760736196319, Blast_Score=195, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI17542784, Length=245, Percent_Identity=34.6938775510204, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6321295, Length=314, Percent_Identity=34.7133757961783, Blast_Score=153, Evalue=4e-38,
Organism=Drosophila melanogaster, GI19921226, Length=260, Percent_Identity=38.4615384615385, Blast_Score=169, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 37464; Mature: 37333

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADPDFWGDQQKAQTIINEANALKDYVNTYKSLSESHEELQMTHDLLKEDPDQDLQNELV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
NELKSLTKQFNEFELQLLLSEPYDKNNAILELHPGAGGTESQDWGSMLLRMYTRWAERRG
HHHHHHHHHHHHHEEEEEECCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCC
FKVETLDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHRLVRISPFDSSGRRHTSFVSC
CEEEEECCCCCCCCCCCEEEEEEECCCCEEEEEHHHCHHHEEEECCCCCCCCCCCEEEEE
DVMPEFNEEIDIEIRTEDIKVDTYRASGAGGQHVNTTDSAVRITHLPTGVIVTCQTERSQ
ECCCCCCCCEEEEEEECEEEEEEEEECCCCCCCCCCCCCEEEEEECCCCEEEEEECHHHH
IKNRERAMKMLKSKLYQRRIEEQQAQLDEIRGEQKEIGWGSQIRSYVFHPYSLVKDHRTN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHCCCHHHHHHCCCC
TEMGNVQAVMDGDIDSFIDAYLRSKLS
CCCCCEEEEECCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
ADPDFWGDQQKAQTIINEANALKDYVNTYKSLSESHEELQMTHDLLKEDPDQDLQNELV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
NELKSLTKQFNEFELQLLLSEPYDKNNAILELHPGAGGTESQDWGSMLLRMYTRWAERRG
HHHHHHHHHHHHHEEEEEECCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCC
FKVETLDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHRLVRISPFDSSGRRHTSFVSC
CEEEEECCCCCCCCCCCEEEEEEECCCCEEEEEHHHCHHHEEEECCCCCCCCCCCEEEEE
DVMPEFNEEIDIEIRTEDIKVDTYRASGAGGQHVNTTDSAVRITHLPTGVIVTCQTERSQ
ECCCCCCCCEEEEEEECEEEEEEEEECCCCCCCCCCCCCEEEEEECCCCEEEEEECHHHH
IKNRERAMKMLKSKLYQRRIEEQQAQLDEIRGEQKEIGWGSQIRSYVFHPYSLVKDHRTN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHCCCHHHHHHCCCC
TEMGNVQAVMDGDIDSFIDAYLRSKLS
CCCCCEEEEECCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9696765; 9384377; 1901557; 1408743 [H]