Click here to switch to the map view.

The map label for this gene is cwlO [H]

Identifier: 52787447

GI number: 52787447

Start: 3588514

End: 3589764

Strand: Reverse

Name: cwlO [H]

Synonym: BLi03767

Alternate gene names: 52787447

Gene position: 3589764-3588514 (Counterclockwise)

Preceding gene: 52787448

Following gene: 52787446

Centisome position: 85.01

GC content: 45.72

Gene sequence:

>1251_bases
TTGAAAAGAAAGCTTATGACGCTGGGGCTGACCGCTGTGCTCGGATCGTCGGCAGTGCTGATCCCCTTAAAAAGCAACCA
TGCTTTAGCTTATGAAGACTTGGAGAAAAAAAAGAGCGACGTTCAAAGCAAAAAATCTGAAAACGAGTCAAAACTCGAAA
AAACGAAACAGGAGCTTTCAGAGCTTGAATCAAAGGAAGCAAGCCTGAGAAGCGAGATCGAGAAAATCGACCGTAAGATG
ACGGACACGAATGAAAAGCTGGAAAAGAAAAATGAAGAAATCGATAAAACAAAGAAAAGCATCGAAGAGCTGAAAAAGCA
AATAAAAAAGCTTAAAGAAAAGATTGAGAAACGCAATAAAATATTGAAAGACCGTGTTCGCTCAATGCAGGAAAACGGCG
GTTCTGTTCAATACATAGATGTGCTTCTCGGCTCGAAAAGCTTCGGGGATTTCATCAGCCGCGCGGGTGCGGTTTCGACA
ATTGTAGAAGCTGATAAAGACCTGATGGAAGAGCAAAAACGCGATCTTCAGCTTGTCGAGGAAAAGGAAACTAAGCTGAG
CAAGGATCTTGAAAGCCTTGAAAAAGCGCTCAAAGACCTGGAAGATTTAAAAAAGACGCTCGACAAGCAGCAAAAAGAGA
AAGCAAATGTCATGAAAAAAGTGAAGCAAGATAAAGATCATGCACATGAAGAACTTGGTTCACTCGAAAATGAAGCAGAA
ATTTTAAAGCGCCAGGATGAAGCGATCAAAGCCGAAGAGGCGCATCGCAAAAAGCAGGAAGCTGAAGCGAGCCGCCAGGC
TTCAAGCGGCCAATCGGAAGCTTCCTCATCTAAACCGTCGGCACCAAGCAGCTCAGGCTTTATCCGCCCTGCTTCCGGCA
CATTTACTTCCGGGTTCGGAAAGCGTTCGCTCGGAAATCACTTCGGCGTAGACATCGCCAAGCGCGGTTCAGGCGTTCCG
ATTTATGCGGCCGCCGCCGGAACGGTCTATAACGCCCATTACTCGTCCAGCTACGGCAATGTTGTGTTTATTACCCATAA
CATCAACGGGCAAACATATCAGACGGTCTACGCTCACATGTCTTCAATTAAAGTGCAGACAGGCCAGCGTGTTGAACAGG
GACAAGTGATCGGAACGATGGGAAATACAGGCCAATCCTACGGGCAGCATCTTCACTTTGAAATTCATAAAGGCCTTTGG
AACAACGCGAAATCCAATGCTGTTGATCCGGCCAATTATATACCGCTTTAA

Upstream 100 bases:

>100_bases
AAGTTTAAACCGGCGCTTTCGCCGGTTGTATCATGTTTAAAGCTGGGATGCCTGCAAAGCGCCGGGCTCCCTTGATTCTG
AAGGCAAGGAGGAACCAAGG

Downstream 100 bases:

>100_bases
AATGAAAGCCGATTCGTCCGGAGATGGACTGAATCGGCTTTTTGTTATTCAAACTGGGTTTTTCTCAAAATTTATTCTTG
TCAGAAAATAACACAAGGTA

Product: hypothetical protein

Products: NA

Alternate protein names: D-gamma-glutamyl-meso-diaminopimelyl DL-endopeptidase; PSPA2 [H]

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MKRKLMTLGLTAVLGSSAVLIPLKSNHALAYEDLEKKKSDVQSKKSENESKLEKTKQELSELESKEASLRSEIEKIDRKM
TDTNEKLEKKNEEIDKTKKSIEELKKQIKKLKEKIEKRNKILKDRVRSMQENGGSVQYIDVLLGSKSFGDFISRAGAVST
IVEADKDLMEEQKRDLQLVEEKETKLSKDLESLEKALKDLEDLKKTLDKQQKEKANVMKKVKQDKDHAHEELGSLENEAE
ILKRQDEAIKAEEAHRKKQEAEASRQASSGQSEASSSKPSAPSSSGFIRPASGTFTSGFGKRSLGNHFGVDIAKRGSGVP
IYAAAAGTVYNAHYSSSYGNVVFITHNINGQTYQTVYAHMSSIKVQTGQRVEQGQVIGTMGNTGQSYGQHLHFEIHKGLW
NNAKSNAVDPANYIPL

Sequences:

>Translated_416_residues
MKRKLMTLGLTAVLGSSAVLIPLKSNHALAYEDLEKKKSDVQSKKSENESKLEKTKQELSELESKEASLRSEIEKIDRKM
TDTNEKLEKKNEEIDKTKKSIEELKKQIKKLKEKIEKRNKILKDRVRSMQENGGSVQYIDVLLGSKSFGDFISRAGAVST
IVEADKDLMEEQKRDLQLVEEKETKLSKDLESLEKALKDLEDLKKTLDKQQKEKANVMKKVKQDKDHAHEELGSLENEAE
ILKRQDEAIKAEEAHRKKQEAEASRQASSGQSEASSSKPSAPSSSGFIRPASGTFTSGFGKRSLGNHFGVDIAKRGSGVP
IYAAAAGTVYNAHYSSSYGNVVFITHNINGQTYQTVYAHMSSIKVQTGQRVEQGQVIGTMGNTGQSYGQHLHFEIHKGLW
NNAKSNAVDPANYIPL
>Mature_416_residues
MKRKLMTLGLTAVLGSSAVLIPLKSNHALAYEDLEKKKSDVQSKKSENESKLEKTKQELSELESKEASLRSEIEKIDRKM
TDTNEKLEKKNEEIDKTKKSIEELKKQIKKLKEKIEKRNKILKDRVRSMQENGGSVQYIDVLLGSKSFGDFISRAGAVST
IVEADKDLMEEQKRDLQLVEEKETKLSKDLESLEKALKDLEDLKKTLDKQQKEKANVMKKVKQDKDHAHEELGSLENEAE
ILKRQDEAIKAEEAHRKKQEAEASRQASSGQSEASSSKPSAPSSSGFIRPASGTFTSGFGKRSLGNHFGVDIAKRGSGVP
IYAAAAGTVYNAHYSSSYGNVVFITHNINGQTYQTVYAHMSSIKVQTGQRVEQGQVIGTMGNTGQSYGQHLHFEIHKGLW
NNAKSNAVDPANYIPL

Specific function: The C-terminal part of CwlO shows a cell wall hydrolytic DL-endopeptidase activity [H]

COG id: COG3883

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Secreted. Secreted, cell wall [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase C40 family [H]

Homologues:

Organism=Escherichia coli, GI87082174, Length=163, Percent_Identity=33.7423312883436, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI87081989, Length=135, Percent_Identity=37.037037037037, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1789099, Length=128, Percent_Identity=39.84375, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000064 [H]

Pfam domain/function: PF00877 NLPC_P60 [H]

EC number: NA

Molecular weight: Translated: 46339; Mature: 46339

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRKLMTLGLTAVLGSSAVLIPLKSNHALAYEDLEKKKSDVQSKKSENESKLEKTKQELS
CCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
ELESKEASLRSEIEKIDRKMTDTNEKLEKKNEEIDKTKKSIEELKKQIKKLKEKIEKRNK
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILKDRVRSMQENGGSVQYIDVLLGSKSFGDFISRAGAVSTIVEADKDLMEEQKRDLQLVE
HHHHHHHHHHHCCCCEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKETKLSKDLESLEKALKDLEDLKKTLDKQQKEKANVMKKVKQDKDHAHEELGSLENEAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
ILKRQDEAIKAEEAHRKKQEAEASRQASSGQSEASSSKPSAPSSSGFIRPASGTFTSGFG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCC
KRSLGNHFGVDIAKRGSGVPIYAAAAGTVYNAHYSSSYGNVVFITHNINGQTYQTVYAHM
CHHCCCCCCCCHHCCCCCCEEEEECCCCEEECCCCCCCCCEEEEEECCCCCHHHHHHHHH
SSIKVQTGQRVEQGQVIGTMGNTGQSYGQHLHFEIHKGLWNNAKSNAVDPANYIPL
HHHHHHCCCCHHCCCEEECCCCCCHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKRKLMTLGLTAVLGSSAVLIPLKSNHALAYEDLEKKKSDVQSKKSENESKLEKTKQELS
CCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
ELESKEASLRSEIEKIDRKMTDTNEKLEKKNEEIDKTKKSIEELKKQIKKLKEKIEKRNK
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILKDRVRSMQENGGSVQYIDVLLGSKSFGDFISRAGAVSTIVEADKDLMEEQKRDLQLVE
HHHHHHHHHHHCCCCEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKETKLSKDLESLEKALKDLEDLKKTLDKQQKEKANVMKKVKQDKDHAHEELGSLENEAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
ILKRQDEAIKAEEAHRKKQEAEASRQASSGQSEASSSKPSAPSSSGFIRPASGTFTSGFG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCC
KRSLGNHFGVDIAKRGSGVPIYAAAAGTVYNAHYSSSYGNVVFITHNINGQTYQTVYAHM
CHHCCCCCCCCHHCCCCCCEEEEECCCCEEECCCCCCCCCEEEEEECCCCCHHHHHHHHH
SSIKVQTGQRVEQGQVIGTMGNTGQSYGQHLHFEIHKGLWNNAKSNAVDPANYIPL
HHHHHHCCCCHHCCCEEECCCCCCHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 3145906 [H]