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The map label for this gene is sacB [H]

Identifier: 52787390

GI number: 52787390

Start: 3535084

End: 3536532

Strand: Direct

Name: sacB [H]

Synonym: BLi03706

Alternate gene names: 52787390

Gene position: 3535084-3536532 (Clockwise)

Preceding gene: 52787389

Following gene: 52787391

Centisome position: 83.72

GC content: 45.13

Gene sequence:

>1449_bases
ATGAACATCAAAAACATTGCTAAAAAAGCGTCAGCCTTAACCGTTGCTGCGGCACTGCTGGCCGGAGGTGCGCCGCAAAC
CTTTGCAAAAGAAACGCAGGATTACAAGAAAAGCTACGGATTTTCTCATATCACAAGACATGACATGCTGAAAATTCCCG
AGCAGCAAAAGAGCGAACAATTTAAAGTTCCTCAATTCGATCCGAAAACAATCAAAAACATCCCTTCTGCAAAAGGGTAT
AACAAAAATGGAGAGCTGATCGATTTAGACGTATGGGACAGCTGGCCGCTGCAAAATGCCGACGGGACGGTTGCTACATA
CCACGGCTACAATCTTGTTTTCGCGCTGGCGGGCGATCCGAAAGACGTCGATGACACATCCATCTATTTGTTCTATCAAA
AGAAAGGCGAAACTTCTATCGACAGCTGGAAAAACGCCGGCAGAGTGTTTAAAGACAGCGACAAATTTGTTCCAGACGAT
CCGTACCTCAAACATCAAACACAGGAATGGTCAGGTTCTGCCACGCTGACAAAAGACGGAAAAGTCCGACTGTTTTACAC
AGCTTTTTCCGGCACGCAATACGGCAAGCAGACGCTGACAACAGCTCAGGTCAATTTCTCTCAGCCGGATTCGGACACGC
TCAAAATTGACGGTGTAGAAGATCATAAATCGGTCTTTGACGGCGCCGACGGCACGGTATACCAAAACGTTCAGCAATTC
ATTGACGAAGGAAACTACAGCTCCGGCGACAACCATACGATGAGAGACCCGCATTATGTGGAAGACCGCGGCCATAAATA
TCTCGTATTTGAAGCCAATACGGGAACAAAAACCGGCTACCAAGGAGAAGACTCCCTATTCAACAGAGCCTACTACGGGG
GCAGCAAGAAGTTCTTTAAAGAAGAAAGCAGCAAGCTGCTGCAAGGTGCGAACAAAAAGAACGCTTCGCTGGCTAACGGC
GCTCTCGGAATCATCGAATTAAATAACGATTATACACTGAAAAAAGTCATGAAGCCTTTGATCGCCTCCAATACGGTGAC
AGATGAAATCGAACGGGCCAACCTCTTCAAAATGAATGGAAAATGGTATCTGTTCACAGATTCAAGAGGATCAAAAATGA
CAATTGACGGCATCGGTTCAAAAGACATTTATATGCTCGGCTATGTATCAGGTTCATTAACCGGACCATTCAAGCCTTTA
AACAAATCCGGACTTGTTTTGCATATGGACCAGGATTACAATGACATCACGTTTACTTATTCACACTTTGCCGTACCGCA
GAAAAAAGGCGACGAAGTCGTCATTACAAGCTACATCACAAACAGAGGGATTTCGAACGAGCATCACGCCACGTTTGCAC
CAAGCTTTTTGCTGAAGATCAAAGGATCAAAAACATCCGTTGTCAAAAACAGCATCCTTGAACAGGGACAACTAACGGTA
AACAAATAA

Upstream 100 bases:

>100_bases
TTCTGTTCTTCAGGACAATGCCGCAGTTAGGAGGGATGATTGGGAACGTTCATGTTGTCAGAAGCAATCTATTACATATT
GAAAAAGGGAGGAAATATTG

Downstream 100 bases:

>100_bases
AACGAACGAAGGAAAATGCCGGTGAACATCGGCATTTTCTTTCGTATGTCATCTTAGAATAAAGCGGGGAATTTTCTTGA
AGAAGGCAGTATATAAGCGG

Product: SacB

Products: NA

Alternate protein names: Beta-D-fructofuranosyl transferase; Sucrose 6-fructosyl transferase [H]

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MNIKNIAKKASALTVAAALLAGGAPQTFAKETQDYKKSYGFSHITRHDMLKIPEQQKSEQFKVPQFDPKTIKNIPSAKGY
NKNGELIDLDVWDSWPLQNADGTVATYHGYNLVFALAGDPKDVDDTSIYLFYQKKGETSIDSWKNAGRVFKDSDKFVPDD
PYLKHQTQEWSGSATLTKDGKVRLFYTAFSGTQYGKQTLTTAQVNFSQPDSDTLKIDGVEDHKSVFDGADGTVYQNVQQF
IDEGNYSSGDNHTMRDPHYVEDRGHKYLVFEANTGTKTGYQGEDSLFNRAYYGGSKKFFKEESSKLLQGANKKNASLANG
ALGIIELNNDYTLKKVMKPLIASNTVTDEIERANLFKMNGKWYLFTDSRGSKMTIDGIGSKDIYMLGYVSGSLTGPFKPL
NKSGLVLHMDQDYNDITFTYSHFAVPQKKGDEVVITSYITNRGISNEHHATFAPSFLLKIKGSKTSVVKNSILEQGQLTV
NK

Sequences:

>Translated_482_residues
MNIKNIAKKASALTVAAALLAGGAPQTFAKETQDYKKSYGFSHITRHDMLKIPEQQKSEQFKVPQFDPKTIKNIPSAKGY
NKNGELIDLDVWDSWPLQNADGTVATYHGYNLVFALAGDPKDVDDTSIYLFYQKKGETSIDSWKNAGRVFKDSDKFVPDD
PYLKHQTQEWSGSATLTKDGKVRLFYTAFSGTQYGKQTLTTAQVNFSQPDSDTLKIDGVEDHKSVFDGADGTVYQNVQQF
IDEGNYSSGDNHTMRDPHYVEDRGHKYLVFEANTGTKTGYQGEDSLFNRAYYGGSKKFFKEESSKLLQGANKKNASLANG
ALGIIELNNDYTLKKVMKPLIASNTVTDEIERANLFKMNGKWYLFTDSRGSKMTIDGIGSKDIYMLGYVSGSLTGPFKPL
NKSGLVLHMDQDYNDITFTYSHFAVPQKKGDEVVITSYITNRGISNEHHATFAPSFLLKIKGSKTSVVKNSILEQGQLTV
NK
>Mature_482_residues
MNIKNIAKKASALTVAAALLAGGAPQTFAKETQDYKKSYGFSHITRHDMLKIPEQQKSEQFKVPQFDPKTIKNIPSAKGY
NKNGELIDLDVWDSWPLQNADGTVATYHGYNLVFALAGDPKDVDDTSIYLFYQKKGETSIDSWKNAGRVFKDSDKFVPDD
PYLKHQTQEWSGSATLTKDGKVRLFYTAFSGTQYGKQTLTTAQVNFSQPDSDTLKIDGVEDHKSVFDGADGTVYQNVQQF
IDEGNYSSGDNHTMRDPHYVEDRGHKYLVFEANTGTKTGYQGEDSLFNRAYYGGSKKFFKEESSKLLQGANKKNASLANG
ALGIIELNNDYTLKKVMKPLIASNTVTDEIERANLFKMNGKWYLFTDSRGSKMTIDGIGSKDIYMLGYVSGSLTGPFKPL
NKSGLVLHMDQDYNDITFTYSHFAVPQKKGDEVVITSYITNRGISNEHHATFAPSFLLKIKGSKTSVVKNSILEQGQLTV
NK

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 68 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003469 [H]

Pfam domain/function: PF02435 Glyco_hydro_68 [H]

EC number: =2.4.1.10 [H]

Molecular weight: Translated: 53717; Mature: 53717

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIKNIAKKASALTVAAALLAGGAPQTFAKETQDYKKSYGFSHITRHDMLKIPEQQKSEQ
CCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHCHHHHHCCCCHHHHCC
FKVPQFDPKTIKNIPSAKGYNKNGELIDLDVWDSWPLQNADGTVATYHGYNLVFALAGDP
CCCCCCCHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEECCEEEEEECCCC
KDVDDTSIYLFYQKKGETSIDSWKNAGRVFKDSDKFVPDDPYLKHQTQEWSGSATLTKDG
CCCCCCEEEEEEECCCCCCHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCCEEEECCC
KVRLFYTAFSGTQYGKQTLTTAQVNFSQPDSDTLKIDGVEDHKSVFDGADGTVYQNVQQF
CEEEEEEEECCCCCCCCEEEEEEEECCCCCCCEEEECCCHHHHHHHCCCCCHHHHHHHHH
IDEGNYSSGDNHTMRDPHYVEDRGHKYLVFEANTGTKTGYQGEDSLFNRAYYGGSKKFFK
HHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHEECCCHHHHH
EESSKLLQGANKKNASLANGALGIIELNNDYTLKKVMKPLIASNTVTDEIERANLFKMNG
HHHHHHHHCCCCCCCEECCCCEEEEEECCCCCHHHHHHHHHHCCCHHHHHCCCCEEEECC
KWYLFTDSRGSKMTIDGIGSKDIYMLGYVSGSLTGPFKPLNKSGLVLHMDQDYNDITFTY
EEEEEECCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCEEEEE
SHFAVPQKKGDEVVITSYITNRGISNEHHATFAPSFLLKIKGSKTSVVKNSILEQGQLTV
EEEECCCCCCCCEEEEEEECCCCCCCCCCCEECCEEEEEECCCCHHHHHHHHHHCCCEEE
NK
CC
>Mature Secondary Structure
MNIKNIAKKASALTVAAALLAGGAPQTFAKETQDYKKSYGFSHITRHDMLKIPEQQKSEQ
CCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHCHHHHHCCCCHHHHCC
FKVPQFDPKTIKNIPSAKGYNKNGELIDLDVWDSWPLQNADGTVATYHGYNLVFALAGDP
CCCCCCCHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEECCEEEEEECCCC
KDVDDTSIYLFYQKKGETSIDSWKNAGRVFKDSDKFVPDDPYLKHQTQEWSGSATLTKDG
CCCCCCEEEEEEECCCCCCHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCCEEEECCC
KVRLFYTAFSGTQYGKQTLTTAQVNFSQPDSDTLKIDGVEDHKSVFDGADGTVYQNVQQF
CEEEEEEEECCCCCCCCEEEEEEEECCCCCCCEEEECCCHHHHHHHCCCCCHHHHHHHHH
IDEGNYSSGDNHTMRDPHYVEDRGHKYLVFEANTGTKTGYQGEDSLFNRAYYGGSKKFFK
HHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHEECCCHHHHH
EESSKLLQGANKKNASLANGALGIIELNNDYTLKKVMKPLIASNTVTDEIERANLFKMNG
HHHHHHHHCCCCCCCEECCCCEEEEEECCCCCHHHHHHHHHHCCCHHHHHCCCCEEEECC
KWYLFTDSRGSKMTIDGIGSKDIYMLGYVSGSLTGPFKPLNKSGLVLHMDQDYNDITFTY
EEEEEECCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCEEEEE
SHFAVPQKKGDEVVITSYITNRGISNEHHATFAPSFLLKIKGSKTSVVKNSILEQGQLTV
EEEECCCCCCCCEEEEEEECCCCCCCCCCCEECCEEEEEECCCCHHHHHHHHHHCCCEEE
NK
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2993818; 9384377; 6424671; 2428811 [H]