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The map label for this gene is yvfH [H]

Identifier: 52787361

GI number: 52787361

Start: 3503565

End: 3505256

Strand: Direct

Name: yvfH [H]

Synonym: BLi03677

Alternate gene names: 52787361

Gene position: 3503565-3505256 (Clockwise)

Preceding gene: 52787354

Following gene: 52787363

Centisome position: 82.97

GC content: 51.36

Gene sequence:

>1692_bases
ATGGGATGGACGCAAGATTATACTCCGCTGGGGAACAGTTTGCTGCTCTCTTCGCTTGCAGCGCTTATACCGATTTTGTA
TTTTTTCTGGGCTCTGGCAATCAAAAGAATGAAAGGGCATACAGCAGGCTTGACGACTTTGCTGATCGCACTCGTGCTGG
CCGTTTTCGTATACGGCATGCCCGCTCAGCAGGCTGTGATGTCGGCTTCGCAAGGCGCGGTTTACGGGCTGCTGCCGATC
GGCTGGATTATCGTGACTTCAGTTTTTTTGTACAAGCTTACGGTGAAAACCGGCCAATTTGAAATCATCAGGAGTTCGGT
ATTGTCGATCACAGATGACCGCCGTTTGCAAGCACTTTTGATAGCGTTTTCATTCGGGGCATTTTTGGAAGGTGCGGCAG
GCTTCGGCGCCCCTGTCGCGATATCGGCTGCATTGCTTGTCGGCCTCGGCTTTAATCCGTTGTATGCGGCGGGCATATGC
CTGATCGCCAACACGGCGCCTGTCGCTTTCGGAGCGATCGGCATCCCGATTATCGCGGTTGAAGGGCCGACTGGGATTCC
TGCGATGGACATTTCAAAAATGGTCGGACGCCAGCTGCCGTTTTTATCTGTATTTATTCCGTTTTATTTGATCATCATCA
TGAGCGGTTTCAAAAGGGCTTTGGAAGTGCTGCCGGCCATTCTCGTGTCGGGCGTCTCTTTTGCCGTTACACAGTATGTA
AGCTCAAACTTCCTGGGGCCTGAGCTCCCGGATATTTTGTCAGCTATCGTATCGCTTATCGCTCTCGCTGTATTCTTAAA
ATTTTGGAAACCGAAAACGACATTCAGATTCTCACAAGAAAAGGAAATGGCCGCTGCAGCGGAGGCGCAGCAGCTTGAAC
ATCAAAAGGCCTCATACTCGGCGGGGCAGATTTTCAAGGCATGGACACCGTTTTTGCTGCTCACGGCAATGGTCTCCATC
TGGGGAATTCCATCTGTCAAACAGGCCTTGACGGGCCATTATGAAGGTTCAAATCCATTGCTGCTGTTCATAAATGATAT
CGGCGGCAGGCTGACGTTTGCACCCGGCGTGCCTTTTTTGCACAATCAAATTCTCGATGGTTCCGGAAAACCGCTTGAGG
CTCTTTACAAACTTGATCTCGCTGGATCGGCAGGTACGGCGATCCTGATTGCCGCCGTACTGACAAAGTTTGTCACAGGC
ATGCCTTGGAAAGAATGGGGCGCCGTATTTACAGATTCGCTGAAGGAACTCGGCAAGCCGATATTAACGATCGCTTCCGT
CGTCGGTTTTGCCTATGTCACCAATTCTTCGGGCATGAGCACAACACTGGGCATGTCCCTTGCGATGACCGGTTCAATGT
TCGCATTCTTTTCTCCCGTTCTCGGCTGGCTCGGTGTGTTTATAACCGGTTCAGATACGTCGGCCAATTTGCTGTTCGGA
AGCCTGCAAAAAATGACGGCACAGTCAGTCGGAATGGAGCCGGTGCTGTCTGTGGCGGCCAACTCTTCAGGCGGCGTCAC
AGGAAAAATGATCTCTCCGCAGTCGATTGCGGTCGCCTGCGCAGCCGTCGGACTGGCAGGAAAGGAATCCGACTTGCTTC
GCTTTACACTGAAACACAGTCTGTTTCTTCTTTTCCTCGTTTGCATGATCACTTTCCTGCAGCATCATCTGTTTTCGTGG
ATGATCCCGTAA

Upstream 100 bases:

>100_bases
TTTATGACATTGAGAAATATAATGCTAAATGATCAGTCTTATCATCATGTCATCAGATGACTAGATGAGGCTTTTTTGAC
GAACAAAGGGGGAATTGGAG

Downstream 100 bases:

>100_bases
AAAAGCCGCCGGCGCTCGTGCCGGACGGCTTCTTTATTGATATCTCTTTCTTACAGACGAAGGCAGTATATTGAGCTGAT
CACGGTATTTGGCGACGGTC

Product: YvfH

Products: lactate [Cytoplasm]; Proton [Cytoplasm]; glycolate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 563; Mature: 562

Protein sequence:

>563_residues
MGWTQDYTPLGNSLLLSSLAALIPILYFFWALAIKRMKGHTAGLTTLLIALVLAVFVYGMPAQQAVMSASQGAVYGLLPI
GWIIVTSVFLYKLTVKTGQFEIIRSSVLSITDDRRLQALLIAFSFGAFLEGAAGFGAPVAISAALLVGLGFNPLYAAGIC
LIANTAPVAFGAIGIPIIAVEGPTGIPAMDISKMVGRQLPFLSVFIPFYLIIIMSGFKRALEVLPAILVSGVSFAVTQYV
SSNFLGPELPDILSAIVSLIALAVFLKFWKPKTTFRFSQEKEMAAAAEAQQLEHQKASYSAGQIFKAWTPFLLLTAMVSI
WGIPSVKQALTGHYEGSNPLLLFINDIGGRLTFAPGVPFLHNQILDGSGKPLEALYKLDLAGSAGTAILIAAVLTKFVTG
MPWKEWGAVFTDSLKELGKPILTIASVVGFAYVTNSSGMSTTLGMSLAMTGSMFAFFSPVLGWLGVFITGSDTSANLLFG
SLQKMTAQSVGMEPVLSVAANSSGGVTGKMISPQSIAVACAAVGLAGKESDLLRFTLKHSLFLLFLVCMITFLQHHLFSW
MIP

Sequences:

>Translated_563_residues
MGWTQDYTPLGNSLLLSSLAALIPILYFFWALAIKRMKGHTAGLTTLLIALVLAVFVYGMPAQQAVMSASQGAVYGLLPI
GWIIVTSVFLYKLTVKTGQFEIIRSSVLSITDDRRLQALLIAFSFGAFLEGAAGFGAPVAISAALLVGLGFNPLYAAGIC
LIANTAPVAFGAIGIPIIAVEGPTGIPAMDISKMVGRQLPFLSVFIPFYLIIIMSGFKRALEVLPAILVSGVSFAVTQYV
SSNFLGPELPDILSAIVSLIALAVFLKFWKPKTTFRFSQEKEMAAAAEAQQLEHQKASYSAGQIFKAWTPFLLLTAMVSI
WGIPSVKQALTGHYEGSNPLLLFINDIGGRLTFAPGVPFLHNQILDGSGKPLEALYKLDLAGSAGTAILIAAVLTKFVTG
MPWKEWGAVFTDSLKELGKPILTIASVVGFAYVTNSSGMSTTLGMSLAMTGSMFAFFSPVLGWLGVFITGSDTSANLLFG
SLQKMTAQSVGMEPVLSVAANSSGGVTGKMISPQSIAVACAAVGLAGKESDLLRFTLKHSLFLLFLVCMITFLQHHLFSW
MIP
>Mature_562_residues
GWTQDYTPLGNSLLLSSLAALIPILYFFWALAIKRMKGHTAGLTTLLIALVLAVFVYGMPAQQAVMSASQGAVYGLLPIG
WIIVTSVFLYKLTVKTGQFEIIRSSVLSITDDRRLQALLIAFSFGAFLEGAAGFGAPVAISAALLVGLGFNPLYAAGICL
IANTAPVAFGAIGIPIIAVEGPTGIPAMDISKMVGRQLPFLSVFIPFYLIIIMSGFKRALEVLPAILVSGVSFAVTQYVS
SNFLGPELPDILSAIVSLIALAVFLKFWKPKTTFRFSQEKEMAAAAEAQQLEHQKASYSAGQIFKAWTPFLLLTAMVSIW
GIPSVKQALTGHYEGSNPLLLFINDIGGRLTFAPGVPFLHNQILDGSGKPLEALYKLDLAGSAGTAILIAAVLTKFVTGM
PWKEWGAVFTDSLKELGKPILTIASVVGFAYVTNSSGMSTTLGMSLAMTGSMFAFFSPVLGWLGVFITGSDTSANLLFGS
LQKMTAQSVGMEPVLSVAANSSGGVTGKMISPQSIAVACAAVGLAGKESDLLRFTLKHSLFLLFLVCMITFLQHHLFSWM
IP

Specific function: Is the principal permease for the uptake of L-lactate in B.subtilis [H]

COG id: COG1620

COG function: function code C; L-lactate permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lactate permease family [H]

Homologues:

Organism=Escherichia coli, GI1789347, Length=573, Percent_Identity=54.6247818499127, Blast_Score=559, Evalue=1e-160,
Organism=Escherichia coli, GI1790031, Length=566, Percent_Identity=52.2968197879859, Blast_Score=537, Evalue=1e-154,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003804 [H]

Pfam domain/function: PF02652 Lactate_perm [H]

EC number: NA

Molecular weight: Translated: 59840; Mature: 59708

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGWTQDYTPLGNSLLLSSLAALIPILYFFWALAIKRMKGHTAGLTTLLIALVLAVFVYGM
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCC
PAQQAVMSASQGAVYGLLPIGWIIVTSVFLYKLTVKTGQFEIIRSSVLSITDDRRLQALL
CHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHH
IAFSFGAFLEGAAGFGAPVAISAALLVGLGFNPLYAAGICLIANTAPVAFGAIGIPIIAV
HHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHCHHHEECCCCHHHHCCCCEEEEE
EGPTGIPAMDISKMVGRQLPFLSVFIPFYLIIIMSGFKRALEVLPAILVSGVSFAVTQYV
ECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSNFLGPELPDILSAIVSLIALAVFLKFWKPKTTFRFSQEKEMAAAAEAQQLEHQKASYS
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHCCC
AGQIFKAWTPFLLLTAMVSIWGIPSVKQALTGHYEGSNPLLLFINDIGGRLTFAPGVPFL
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCEEEEEECCCCEEEECCCCCHH
HNQILDGSGKPLEALYKLDLAGSAGTAILIAAVLTKFVTGMPWKEWGAVFTDSLKELGKP
HHHHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCHH
ILTIASVVGFAYVTNSSGMSTTLGMSLAMTGSMFAFFSPVLGWLGVFITGSDTSANLLFG
HHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHH
SLQKMTAQSVGMEPVLSVAANSSGGVTGKMISPQSIAVACAAVGLAGKESDLLRFTLKHS
HHHHHHHHHCCCHHHHHHHCCCCCCCEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHH
LFLLFLVCMITFLQHHLFSWMIP
HHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GWTQDYTPLGNSLLLSSLAALIPILYFFWALAIKRMKGHTAGLTTLLIALVLAVFVYGM
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCC
PAQQAVMSASQGAVYGLLPIGWIIVTSVFLYKLTVKTGQFEIIRSSVLSITDDRRLQALL
CHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHH
IAFSFGAFLEGAAGFGAPVAISAALLVGLGFNPLYAAGICLIANTAPVAFGAIGIPIIAV
HHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHCHHHEECCCCHHHHCCCCEEEEE
EGPTGIPAMDISKMVGRQLPFLSVFIPFYLIIIMSGFKRALEVLPAILVSGVSFAVTQYV
ECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSNFLGPELPDILSAIVSLIALAVFLKFWKPKTTFRFSQEKEMAAAAEAQQLEHQKASYS
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHCCC
AGQIFKAWTPFLLLTAMVSIWGIPSVKQALTGHYEGSNPLLLFINDIGGRLTFAPGVPFL
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCEEEEEECCCCEEEECCCCCHH
HNQILDGSGKPLEALYKLDLAGSAGTAILIAAVLTKFVTGMPWKEWGAVFTDSLKELGKP
HHHHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCHH
ILTIASVVGFAYVTNSSGMSTTLGMSLAMTGSMFAFFSPVLGWLGVFITGSDTSANLLFG
HHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHH
SLQKMTAQSVGMEPVLSVAANSSGGVTGKMISPQSIAVACAAVGLAGKESDLLRFTLKHS
HHHHHHHHHCCCHHHHHHHCCCCCCCEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHH
LFLLFLVCMITFLQHHLFSWMIP
HHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lactate [Periplasm]; Proton [Periplasm]; glycolate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + lactate [Periplasm] = Proton [Cytoplasm] + lactate [Cytoplasm] Proton [Periplasm] + glycolate [Periplasm] = Proton [Cytoplasm] + glycolate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 8969506 [H]