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The map label for this gene is ald [H]

Identifier: 52787077

GI number: 52787077

Start: 3235582

End: 3236721

Strand: Direct

Name: ald [H]

Synonym: BLi03382

Alternate gene names: 52787077

Gene position: 3235582-3236721 (Clockwise)

Preceding gene: 52787076

Following gene: 52787080

Centisome position: 76.62

GC content: 54.12

Gene sequence:

>1140_bases
ATGATTATCGGCGTACCGAAAGAAATCAAAAACAATGAAAACCGTGTTGCTTTGACTCCCGGTGCGGCTTCTCAATTGAT
CGCGGCAGGACACCGCGTCATCGTGGAAAAAGACGCGGGGACTGGAAGCGGGTTTGAAAATGAGGATTACGTCTCAGTGG
GAGCCGAGATCTCAGAACAAGCGGAAGCCGTCTGGGAAGCGGCTGAAATGGTGATGAAAGTCAAAGAGCCGCTCCCTGAA
GAATATCCGTATTTTAGAGAAGGTCTCATCTTGTTCACCTACCTGCATCTAGCCGCAGAGCCTTCCTTGGCTGAAGCATT
AAAGCAGAAAGGCGTGACAGCCATCGCCTATGAAACCGTCAGCAATGGCCGTTCCCTTCCTTTGCTGACGCCGATGTCTG
AGGTAGCCGGGCGGATGGCCGCCCAAATCGGCGCGCAGTTCCTCGAAAAGCCGAAAGGCGGAAAAGGCATATTATTGGCC
GGTGTACCCGGAGTCGCGCGCGGGAAAGTGACGATCATCGGCGGAGGCGTTGTCGGCACAAATGCGGCAAAAATCGCGGC
GGGCCTCGGAGCCGACGTGACGATGATCGATTTAAGCGCGGATCGCCTGCGCCAGCTTGATGATCAATTCGGCAATCAAA
TTAAAACATTGATGTCCAATCCGGTCAACATCGCCGATGCCGTAGCGGAAGCCGACCTCCTCATCTGCGCGGTTCTGATC
CCGGGTGCCAAAGCGCCGACTTTAGTGACGGAAGAGATGGTCAAACAGATGAAACCGGGCTCTGTGATTGTTGACGTCGC
AATCGATCAAGGCGGCATCGTCGAGACGGTGGATCACATCACAACCCACGACAATCCGACCTATGTCAAACACGGAGTCG
TCCATTACGCTGTTGCCAACATGCCTGGCGCCGTTCCGCGGACATCTACAGTTGCGCTGACGAATGTAACAGTTCCGTAT
GCGCTTGAAATCGCGAACAAAGGCGCGGAAAAAGCGATTCTTGAAAACCCTGCGCTAAAAGCCGGCGTCAACACAGCAAA
CGGCCATATTACTTATGAAGCCGTAGCCAGGGACTTAAACTATGGCTACGTTCCCGCAGAGCTTGCGATCGAAAATTCGT
CGTCAGCCGCCGGCGCATAG

Upstream 100 bases:

>100_bases
ATGCATGTTTTTTTATTTTTCGATTCAAACAAAGAAAATTCTAAAAAGTCAGTATAAACTAAAGCAACAGCATTTTTACA
GAAAAAGGAGGAATGATCGG

Downstream 100 bases:

>100_bases
CATCAAAAAAACAGAGCCTCGGAAAATATGCCCGAAGCTCTGTCTTTTTTTTATGATTCCGTTTTCCGGCGATCCTGAAT
CAATTTTGCCGCAATTGCAG

Product: hypothetical protein

Products: NA

Alternate protein names: Stage V sporulation protein N [H]

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MIIGVPKEIKNNENRVALTPGAASQLIAAGHRVIVEKDAGTGSGFENEDYVSVGAEISEQAEAVWEAAEMVMKVKEPLPE
EYPYFREGLILFTYLHLAAEPSLAEALKQKGVTAIAYETVSNGRSLPLLTPMSEVAGRMAAQIGAQFLEKPKGGKGILLA
GVPGVARGKVTIIGGGVVGTNAAKIAAGLGADVTMIDLSADRLRQLDDQFGNQIKTLMSNPVNIADAVAEADLLICAVLI
PGAKAPTLVTEEMVKQMKPGSVIVDVAIDQGGIVETVDHITTHDNPTYVKHGVVHYAVANMPGAVPRTSTVALTNVTVPY
ALEIANKGAEKAILENPALKAGVNTANGHITYEAVARDLNYGYVPAELAIENSSSAAGA

Sequences:

>Translated_379_residues
MIIGVPKEIKNNENRVALTPGAASQLIAAGHRVIVEKDAGTGSGFENEDYVSVGAEISEQAEAVWEAAEMVMKVKEPLPE
EYPYFREGLILFTYLHLAAEPSLAEALKQKGVTAIAYETVSNGRSLPLLTPMSEVAGRMAAQIGAQFLEKPKGGKGILLA
GVPGVARGKVTIIGGGVVGTNAAKIAAGLGADVTMIDLSADRLRQLDDQFGNQIKTLMSNPVNIADAVAEADLLICAVLI
PGAKAPTLVTEEMVKQMKPGSVIVDVAIDQGGIVETVDHITTHDNPTYVKHGVVHYAVANMPGAVPRTSTVALTNVTVPY
ALEIANKGAEKAILENPALKAGVNTANGHITYEAVARDLNYGYVPAELAIENSSSAAGA
>Mature_379_residues
MIIGVPKEIKNNENRVALTPGAASQLIAAGHRVIVEKDAGTGSGFENEDYVSVGAEISEQAEAVWEAAEMVMKVKEPLPE
EYPYFREGLILFTYLHLAAEPSLAEALKQKGVTAIAYETVSNGRSLPLLTPMSEVAGRMAAQIGAQFLEKPKGGKGILLA
GVPGVARGKVTIIGGGVVGTNAAKIAAGLGADVTMIDLSADRLRQLDDQFGNQIKTLMSNPVNIADAVAEADLLICAVLI
PGAKAPTLVTEEMVKQMKPGSVIVDVAIDQGGIVETVDHITTHDNPTYVKHGVVHYAVANMPGAVPRTSTVALTNVTVPY
ALEIANKGAEKAILENPALKAGVNTANGHITYEAVARDLNYGYVPAELAIENSSSAAGA

Specific function: This enzyme is a key factor in the assimilation of L- alanine as an energy source through the tricarboxylic acid cycle during sporulation [H]

COG id: COG0686

COG function: function code E; Alanine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=355, Percent_Identity=29.8591549295775, Blast_Score=135, Evalue=8e-32,
Organism=Homo sapiens, GI122939153, Length=355, Percent_Identity=29.8591549295775, Blast_Score=135, Evalue=8e-32,
Organism=Escherichia coli, GI1787887, Length=335, Percent_Identity=32.8358208955224, Blast_Score=136, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17550456, Length=357, Percent_Identity=31.0924369747899, Blast_Score=158, Evalue=6e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR008141 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.4.1.1 [H]

Molecular weight: Translated: 39566; Mature: 39566

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIGVPKEIKNNENRVALTPGAASQLIAAGHRVIVEKDAGTGSGFENEDYVSVGAEISEQ
CEECCCHHHCCCCCEEEECCCCHHHHHHCCCEEEEEECCCCCCCCCCCCCEEECCHHHHH
AEAVWEAAEMVMKVKEPLPEEYPYFREGLILFTYLHLAAEPSLAEALKQKGVTAIAYETV
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEEEEC
SNGRSLPLLTPMSEVAGRMAAQIGAQFLEKPKGGKGILLAGVPGVARGKVTIIGGGVVGT
CCCCCCCEECCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEECCEECC
NAAKIAAGLGADVTMIDLSADRLRQLDDQFGNQIKTLMSNPVNIADAVAEADLLICAVLI
CHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEEEEC
PGAKAPTLVTEEMVKQMKPGSVIVDVAIDQGGIVETVDHITTHDNPTYVKHGVVHYAVAN
CCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCHHHHCHHEEHHHC
MPGAVPRTSTVALTNVTVPYALEIANKGAEKAILENPALKAGVNTANGHITYEAVARDLN
CCCCCCCCCEEEEEECCCCEEEEECCCCHHHHHHCCCCHHCCCCCCCCCEEHHHHHHHCC
YGYVPAELAIENSSSAAGA
CCCCCCEEEEECCCCCCCC
>Mature Secondary Structure
MIIGVPKEIKNNENRVALTPGAASQLIAAGHRVIVEKDAGTGSGFENEDYVSVGAEISEQ
CEECCCHHHCCCCCEEEECCCCHHHHHHCCCEEEEEECCCCCCCCCCCCCEEECCHHHHH
AEAVWEAAEMVMKVKEPLPEEYPYFREGLILFTYLHLAAEPSLAEALKQKGVTAIAYETV
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEEEEC
SNGRSLPLLTPMSEVAGRMAAQIGAQFLEKPKGGKGILLAGVPGVARGKVTIIGGGVVGT
CCCCCCCEECCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEECCEECC
NAAKIAAGLGADVTMIDLSADRLRQLDDQFGNQIKTLMSNPVNIADAVAEADLLICAVLI
CHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEEEEC
PGAKAPTLVTEEMVKQMKPGSVIVDVAIDQGGIVETVDHITTHDNPTYVKHGVVHYAVAN
CCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCHHHHCHHEEHHHC
MPGAVPRTSTVALTNVTVPYALEIANKGAEKAILENPALKAGVNTANGHITYEAVARDLN
CCCCCCCCCEEEEEECCCCEEEEECCCCHHHHHHCCCCHHCCCCCCCCCEEHHHHHHHCC
YGYVPAELAIENSSSAAGA
CCCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8226620; 9384377 [H]