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The map label for this gene is citZ [H]

Identifier: 52786791

GI number: 52786791

Start: 2948446

End: 2949564

Strand: Reverse

Name: citZ [H]

Synonym: BLi03062

Alternate gene names: 52786791

Gene position: 2949564-2948446 (Counterclockwise)

Preceding gene: 52786792

Following gene: 52786790

Centisome position: 69.85

GC content: 47.45

Gene sequence:

>1119_bases
ATGACAGCAACACGCGGCCTTGAAGGGGTAGTCGCCACTACATCATCAGTAAGTTCAATTATTGATGATACTCTTACATA
TGTAGGGTATAACATCGATGATTTGACAGAAAAAGCTAGCTTTGAAGAAGTTGTTTATCTGCTTTGGCATTTAAAGCTGC
CGAACAAAGAGGAGCTTGCCGAGCTTAAAAAACAGCTTTCTGAAAATGCCGGTATCCCCAAAGAAGTCATTGACCATTTC
AAATCCTACCCGAATGGAAAAGTCCACCCGATGGCAGCTCTTCGGACAGCCGTTTCTTTACTCGGGCTGTTTGACGAAGA
AGCAGATCAAATGGATGCGGAAGCAAACTACAGAAAAGCGGTGCGTCTCCAAGCGAAGATGCCTGCGCTTGTCGCTGCTT
TTTCAAGAATCCGCAAAGGGCTTGAGCCGATTGAGCCGAATCCGGAACTCGGCTTTGCAGCCAACTTTCTCTACATGCTG
AATGGAAAAGAACCTTCACCTGTCGAAGTGGAAGCGTTTGACAAAGCTTTGATCCTTCATGCGGATCATGAGCTGAACGC
ATCTACGTTCACAGCCAGAGTATGCGTCGCGACGCTTTCAGACGTTTACTCAGGGATAACGGCCGCAATCGGAGCCTTGA
AAGGTCCGCTTCACGGCGGAGCAAACGAAGCAGTTATGAAAATGCTTTCAGAGATTGGCGAGGTTGAAAACGTAGATTCA
TATATTCACGCCAAGCTTGAAAAGAAAGAAAAGATCATGGGGTTCGGCCACCGCGTATACCGCCAGGGCGATCCCCGCGC
AAAACATTTGAAGGAAATGAGCAGGCGTTTGACGAATTTAACGGGTGAAAGCAAATGGTATGAGATTTCAGAGCGGACTG
AGGAAATCGTCACGTCTCAGAAAAAGCTGCCGCCAAACGTAGACTTTTATTCTGCGTCGGTTTATCATAGCTTAGGAATC
GACCATGATCTGTTTACGCCGATTTTTGCGATCAGCAGGGTATCAGGCTGGCTCGCTCACATTCTTGAACAGTATGAAAA
TAACCGCCTGATTCGTCCGCGGGCTGACTACACCGGCCCTGACATGCAGAGGTTCATTCCGATTGAAGAAAGAAGCTGA

Upstream 100 bases:

>100_bases
TTTTGGATTAAAGAAGATGTATACATCATGCGTTGTTTTTGCTCCTGGGTTTTGGTTTAATTTTAAAAGGGGAAATTTTT
AAAAAAAGGGAGAGATGAGT

Downstream 100 bases:

>100_bases
CCGTTTGAAAAGAGGCTGCTCGAGCTTCGTTCGGCCGGCCTCTCAACGGCTTTTCTATGGAAGCAATGAATTAATGAATC
TATGAAAAAACATGTTTGTC

Product: citrate synthase

Products: NA

Alternate protein names: Citrate synthase II [H]

Number of amino acids: Translated: 372; Mature: 371

Protein sequence:

>372_residues
MTATRGLEGVVATTSSVSSIIDDTLTYVGYNIDDLTEKASFEEVVYLLWHLKLPNKEELAELKKQLSENAGIPKEVIDHF
KSYPNGKVHPMAALRTAVSLLGLFDEEADQMDAEANYRKAVRLQAKMPALVAAFSRIRKGLEPIEPNPELGFAANFLYML
NGKEPSPVEVEAFDKALILHADHELNASTFTARVCVATLSDVYSGITAAIGALKGPLHGGANEAVMKMLSEIGEVENVDS
YIHAKLEKKEKIMGFGHRVYRQGDPRAKHLKEMSRRLTNLTGESKWYEISERTEEIVTSQKKLPPNVDFYSASVYHSLGI
DHDLFTPIFAISRVSGWLAHILEQYENNRLIRPRADYTGPDMQRFIPIEERS

Sequences:

>Translated_372_residues
MTATRGLEGVVATTSSVSSIIDDTLTYVGYNIDDLTEKASFEEVVYLLWHLKLPNKEELAELKKQLSENAGIPKEVIDHF
KSYPNGKVHPMAALRTAVSLLGLFDEEADQMDAEANYRKAVRLQAKMPALVAAFSRIRKGLEPIEPNPELGFAANFLYML
NGKEPSPVEVEAFDKALILHADHELNASTFTARVCVATLSDVYSGITAAIGALKGPLHGGANEAVMKMLSEIGEVENVDS
YIHAKLEKKEKIMGFGHRVYRQGDPRAKHLKEMSRRLTNLTGESKWYEISERTEEIVTSQKKLPPNVDFYSASVYHSLGI
DHDLFTPIFAISRVSGWLAHILEQYENNRLIRPRADYTGPDMQRFIPIEERS
>Mature_371_residues
TATRGLEGVVATTSSVSSIIDDTLTYVGYNIDDLTEKASFEEVVYLLWHLKLPNKEELAELKKQLSENAGIPKEVIDHFK
SYPNGKVHPMAALRTAVSLLGLFDEEADQMDAEANYRKAVRLQAKMPALVAAFSRIRKGLEPIEPNPELGFAANFLYMLN
GKEPSPVEVEAFDKALILHADHELNASTFTARVCVATLSDVYSGITAAIGALKGPLHGGANEAVMKMLSEIGEVENVDSY
IHAKLEKKEKIMGFGHRVYRQGDPRAKHLKEMSRRLTNLTGESKWYEISERTEEIVTSQKKLPPNVDFYSASVYHSLGID
HDLFTPIFAISRVSGWLAHILEQYENNRLIRPRADYTGPDMQRFIPIEERS

Specific function: Might regulate the synthesis and function of enzymes involved in later enzymatic steps of Krebs cycle. Loss in activity results in sporulation defect [H]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=373, Percent_Identity=27.3458445040214, Blast_Score=109, Evalue=5e-24,
Organism=Escherichia coli, GI1786527, Length=368, Percent_Identity=37.7717391304348, Blast_Score=255, Evalue=3e-69,
Organism=Escherichia coli, GI1786939, Length=377, Percent_Identity=33.9522546419098, Blast_Score=206, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI17555174, Length=372, Percent_Identity=27.1505376344086, Blast_Score=114, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6319850, Length=371, Percent_Identity=25.6064690026954, Blast_Score=93, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6324328, Length=325, Percent_Identity=25.2307692307692, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6325257, Length=348, Percent_Identity=26.1494252873563, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24640124, Length=374, Percent_Identity=28.0748663101604, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24640126, Length=374, Percent_Identity=28.0748663101604, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI21356863, Length=381, Percent_Identity=27.2965879265092, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011278
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 41622; Mature: 41491

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATRGLEGVVATTSSVSSIIDDTLTYVGYNIDDLTEKASFEEVVYLLWHLKLPNKEELA
CCCCCCCCCHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
ELKKQLSENAGIPKEVIDHFKSYPNGKVHPMAALRTAVSLLGLFDEEADQMDAEANYRKA
HHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
VRLQAKMPALVAAFSRIRKGLEPIEPNPELGFAANFLYMLNGKEPSPVEVEAFDKALILH
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHEEEEECCCCCCCCCHHHHCCEEEEE
ADHELNASTFTARVCVATLSDVYSGITAAIGALKGPLHGGANEAVMKMLSEIGEVENVDS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCHHHHHH
YIHAKLEKKEKIMGFGHRVYRQGDPRAKHLKEMSRRLTNLTGESKWYEISERTEEIVTSQ
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
KKLPPNVDFYSASVYHSLGIDHDLFTPIFAISRVSGWLAHILEQYENNRLIRPRADYTGP
HCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCC
DMQRFIPIEERS
CHHHCCCCCCCC
>Mature Secondary Structure 
TATRGLEGVVATTSSVSSIIDDTLTYVGYNIDDLTEKASFEEVVYLLWHLKLPNKEELA
CCCCCCCCHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
ELKKQLSENAGIPKEVIDHFKSYPNGKVHPMAALRTAVSLLGLFDEEADQMDAEANYRKA
HHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
VRLQAKMPALVAAFSRIRKGLEPIEPNPELGFAANFLYMLNGKEPSPVEVEAFDKALILH
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHEEEEECCCCCCCCCHHHHCCEEEEE
ADHELNASTFTARVCVATLSDVYSGITAAIGALKGPLHGGANEAVMKMLSEIGEVENVDS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCHHHHHH
YIHAKLEKKEKIMGFGHRVYRQGDPRAKHLKEMSRRLTNLTGESKWYEISERTEEIVTSQ
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
KKLPPNVDFYSASVYHSLGIDHDLFTPIFAISRVSGWLAHILEQYENNRLIRPRADYTGP
HCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCC
DMQRFIPIEERS
CHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8045898; 9387221; 9384377 [H]