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The map label for this gene is thrS [H]

Identifier: 52786773

GI number: 52786773

Start: 2927187

End: 2929118

Strand: Reverse

Name: thrS [H]

Synonym: BLi03043

Alternate gene names: 52786773

Gene position: 2929118-2927187 (Counterclockwise)

Preceding gene: 52786774

Following gene: 52786771

Centisome position: 69.37

GC content: 50.36

Gene sequence:

>1932_bases
ATGTCAGATATGGTAAAAATCAAATTTCCTGACGGAGCGGTTAAGGAGTTTGCAAAAGCGACGACAACAGAAGATATCGC
GGCATCAATCAGTCCCGGCTTAAAGAAAAAAGCGATAGCGGGGAAATTAAACGGCAAAGAAATCGATCTGCGCGCACCGA
TCCTTGAGGACGGCGCCATCGAAATCATTACAGAAGGCAGCGAAGAAGCGCTTGAAATCATGCGCCACAGTACTGCACAC
TTAATGGCCCAGGCGATCAAGCGCCTGTATAAAAACGTGAAGCTCGGGGTCGGCCCGGTTATCGAAAACGGCTTCTACTA
CGACGTGGATTTGGAAGAAGCGCTGACTCCGGAAGACCTTCCGAAGATTGAGAAGGAAATGAAAAAAATCGTCGGGGAAA
ATCTGCCGATCGTAAGAAAAGAAGTCAGCCGAGAGGAAGCCAGAGCCCTGTTTGCCGAAATCGGCGATGATCTGAAATTG
GAACTGCTTGATGCGATTCCGGAAGGCGAGACGGTATCGATCTATGAGCAGGGCGAATTCTTCGATCTGTGCCGCGGTGT
TCACGTTCCTTCGACGGGCAAAATCAAGGAGTTCAAGCTGCTGAGCCTTGCGGGGGCATACTGGCGCGGAGACAGCAACA
ACAAGATGCTCCAGCGCATCTATGGGACGGCTTTCTTCAAAAAAGCCGAGCTTGAAGAGCATCTTCGCCTCCTTGAGGAA
GCGAAAGAACGCGACCACAGAAAACTCGGAAAAGAACTGAAGCTGTTCGCCAATTCGCAAAAAGTCGGCCAGGGTCTGCC
GCTTTGGCTGCCAAAGGGTGCGACCATCAGACGCGTCATCGAGCGCTATATCGTTGACAAAGAGCTCGCGCTCGGCTATG
AGCACGTGTACACCCCTGTCCTCGGCAGCAAAGACCTGTATGAAACATCAGGCCACTGGGATCATTATCAAGACGGCATG
TTCCCGCCGATGGAGATGGACAATGAAACACTTGTGCTTCGTCCGATGAACTGTCCGCACCATATGATGATTTATAAAAA
CGACATTCACAGCTACCGTGAGCTTCCGATCCGGATCGCCGAGCTCGGAACGATGCACCGCTATGAAATGTCAGGGGCTC
TTTCAGGGCTGCAGCGCGTCCGCGGAATGACGCTGAATGACGCCCACATTTTCGTCCGTCCAGATCAAATCAAAGACGAG
TTCATTCGAACTGTCCGCCTGATTCAGGATGTATACGAAGACTTCGGACTGGAAGAGTATTCATTCCGCTTGTCTTACCG
CGACCCTGAAGATACAGAGAAATACTACGATGACGATGAAATGTGGAACAAAGCGCAATCGATGCTGAAGGAGGCGATGG
ACGAGATCGGCCATGATTATTACGAAGCTGAAGGGGAAGCGGCCTTCTACGGACCGAAGCTTGACGTTCAGGTCAAAACA
GCAATCGGCAAAGAGGAAACTCTGTCAACTGTTCAGCTGGACTTCCTTCTTCCTGAAAAATTCGATCTGACATATGTCGG
GGAAGACGGAAAACCTCATCGTCCGGTCGTCATTCACAGAGGTGTCGTATCGACAATGGAACGCTTTGTCGCTTTCTTAA
TTGAGGAGCATAAAGGCGCATTGCCGACATGGCTTGCCCCTGTTCAGTTCCAGGTCATCCCTGTATCGCCGTCTGTGCAT
CTTGACTACGCCAAAAAAGTTCAAGAGCGCCTGCAGCGTGAAGGGCTTCGCGTTGAAGTCGATACCCGCGACGAGAAAAT
CGGCTATAAAATCCGTGAAGCGCAGATGCAAAAAATCCCTTACATGCTTGTCGTCGGCGACCAGGAAGTTGAAAACGAAG
CCGTCAACGTCCGCAAATACGGAGAGCAAAAATCAGAAACGGTTGCAATTGCAGACTTTATCAAAAAAGCCGTTGCTGAA
GCAAAGAAATAA

Upstream 100 bases:

>100_bases
TCGGGAAAAAGGGTGGAACCACGAATTCCGTTTATTAATCCTCGTCCCTTTCTTAGGGGGACGGGGATTTTTTATTTTGT
AAAAAAAAGGAGTGACTAGG

Downstream 100 bases:

>100_bases
ACAAGAAAAAAGCATGATTCCCGCCCGGGAGTCATGCTTTTTTGCTTAGCTTGTCCGCTGCTTGTCCGCGGAGGATATCG
AACAGTTCGCTTAAATGGCT

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 643; Mature: 642

Protein sequence:

>643_residues
MSDMVKIKFPDGAVKEFAKATTTEDIAASISPGLKKKAIAGKLNGKEIDLRAPILEDGAIEIITEGSEEALEIMRHSTAH
LMAQAIKRLYKNVKLGVGPVIENGFYYDVDLEEALTPEDLPKIEKEMKKIVGENLPIVRKEVSREEARALFAEIGDDLKL
ELLDAIPEGETVSIYEQGEFFDLCRGVHVPSTGKIKEFKLLSLAGAYWRGDSNNKMLQRIYGTAFFKKAELEEHLRLLEE
AKERDHRKLGKELKLFANSQKVGQGLPLWLPKGATIRRVIERYIVDKELALGYEHVYTPVLGSKDLYETSGHWDHYQDGM
FPPMEMDNETLVLRPMNCPHHMMIYKNDIHSYRELPIRIAELGTMHRYEMSGALSGLQRVRGMTLNDAHIFVRPDQIKDE
FIRTVRLIQDVYEDFGLEEYSFRLSYRDPEDTEKYYDDDEMWNKAQSMLKEAMDEIGHDYYEAEGEAAFYGPKLDVQVKT
AIGKEETLSTVQLDFLLPEKFDLTYVGEDGKPHRPVVIHRGVVSTMERFVAFLIEEHKGALPTWLAPVQFQVIPVSPSVH
LDYAKKVQERLQREGLRVEVDTRDEKIGYKIREAQMQKIPYMLVVGDQEVENEAVNVRKYGEQKSETVAIADFIKKAVAE
AKK

Sequences:

>Translated_643_residues
MSDMVKIKFPDGAVKEFAKATTTEDIAASISPGLKKKAIAGKLNGKEIDLRAPILEDGAIEIITEGSEEALEIMRHSTAH
LMAQAIKRLYKNVKLGVGPVIENGFYYDVDLEEALTPEDLPKIEKEMKKIVGENLPIVRKEVSREEARALFAEIGDDLKL
ELLDAIPEGETVSIYEQGEFFDLCRGVHVPSTGKIKEFKLLSLAGAYWRGDSNNKMLQRIYGTAFFKKAELEEHLRLLEE
AKERDHRKLGKELKLFANSQKVGQGLPLWLPKGATIRRVIERYIVDKELALGYEHVYTPVLGSKDLYETSGHWDHYQDGM
FPPMEMDNETLVLRPMNCPHHMMIYKNDIHSYRELPIRIAELGTMHRYEMSGALSGLQRVRGMTLNDAHIFVRPDQIKDE
FIRTVRLIQDVYEDFGLEEYSFRLSYRDPEDTEKYYDDDEMWNKAQSMLKEAMDEIGHDYYEAEGEAAFYGPKLDVQVKT
AIGKEETLSTVQLDFLLPEKFDLTYVGEDGKPHRPVVIHRGVVSTMERFVAFLIEEHKGALPTWLAPVQFQVIPVSPSVH
LDYAKKVQERLQREGLRVEVDTRDEKIGYKIREAQMQKIPYMLVVGDQEVENEAVNVRKYGEQKSETVAIADFIKKAVAE
AKK
>Mature_642_residues
SDMVKIKFPDGAVKEFAKATTTEDIAASISPGLKKKAIAGKLNGKEIDLRAPILEDGAIEIITEGSEEALEIMRHSTAHL
MAQAIKRLYKNVKLGVGPVIENGFYYDVDLEEALTPEDLPKIEKEMKKIVGENLPIVRKEVSREEARALFAEIGDDLKLE
LLDAIPEGETVSIYEQGEFFDLCRGVHVPSTGKIKEFKLLSLAGAYWRGDSNNKMLQRIYGTAFFKKAELEEHLRLLEEA
KERDHRKLGKELKLFANSQKVGQGLPLWLPKGATIRRVIERYIVDKELALGYEHVYTPVLGSKDLYETSGHWDHYQDGMF
PPMEMDNETLVLRPMNCPHHMMIYKNDIHSYRELPIRIAELGTMHRYEMSGALSGLQRVRGMTLNDAHIFVRPDQIKDEF
IRTVRLIQDVYEDFGLEEYSFRLSYRDPEDTEKYYDDDEMWNKAQSMLKEAMDEIGHDYYEAEGEAAFYGPKLDVQVKTA
IGKEETLSTVQLDFLLPEKFDLTYVGEDGKPHRPVVIHRGVVSTMERFVAFLIEEHKGALPTWLAPVQFQVIPVSPSVHL
DYAKKVQERLQREGLRVEVDTRDEKIGYKIREAQMQKIPYMLVVGDQEVENEAVNVRKYGEQKSETVAIADFIKKAVAEA
KK

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38202255, Length=637, Percent_Identity=38.61852433281, Blast_Score=443, Evalue=1e-124,
Organism=Homo sapiens, GI38570111, Length=621, Percent_Identity=38.3252818035427, Blast_Score=438, Evalue=1e-123,
Organism=Homo sapiens, GI20070344, Length=634, Percent_Identity=35.6466876971609, Blast_Score=376, Evalue=1e-104,
Organism=Escherichia coli, GI1788013, Length=640, Percent_Identity=43.4375, Blast_Score=548, Evalue=1e-157,
Organism=Caenorhabditis elegans, GI71984184, Length=639, Percent_Identity=37.4021909233177, Blast_Score=432, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI71984192, Length=639, Percent_Identity=37.4021909233177, Blast_Score=431, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6322112, Length=656, Percent_Identity=35.8231707317073, Blast_Score=412, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6322654, Length=392, Percent_Identity=36.4795918367347, Blast_Score=266, Evalue=8e-72,
Organism=Drosophila melanogaster, GI24583842, Length=635, Percent_Identity=37.1653543307087, Blast_Score=440, Evalue=1e-123,
Organism=Drosophila melanogaster, GI28574141, Length=635, Percent_Identity=37.1653543307087, Blast_Score=440, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24583839, Length=635, Percent_Identity=37.1653543307087, Blast_Score=440, Evalue=1e-123,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 73536; Mature: 73405

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDMVKIKFPDGAVKEFAKATTTEDIAASISPGLKKKAIAGKLNGKEIDLRAPILEDGAI
CCCCEEEECCCHHHHHHHHHCCHHHHHHHCCCCCHHHHHHCCCCCCEEEEECCCCCCCCE
EIITEGSEEALEIMRHSTAHLMAQAIKRLYKNVKLGVGPVIENGFYYDVDLEEALTPEDL
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCEEEEECHHHCCCCCCH
PKIEKEMKKIVGENLPIVRKEVSREEARALFAEIGDDLKLELLDAIPEGETVSIYEQGEF
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEHHHHCCCCCCEEEEEECCCH
FDLCRGVHVPSTGKIKEFKLLSLAGAYWRGDSNNKMLQRIYGTAFFKKAELEEHLRLLEE
HHHHCCCCCCCCCCCHHEEHHHHHCHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKERDHRKLGKELKLFANSQKVGQGLPLWLPKGATIRRVIERYIVDKELALGYEHVYTPV
HHHHHHHHHHHHHHHHCCCHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHCCHHHHHCCC
LGSKDLYETSGHWDHYQDGMFPPMEMDNETLVLRPMNCPHHMMIYKNDIHSYRELPIRIA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEECHHHHHHHCCEEEE
ELGTMHRYEMSGALSGLQRVRGMTLNDAHIFVRPDQIKDEFIRTVRLIQDVYEDFGLEEY
HHCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCEE
SFRLSYRDPEDTEKYYDDDEMWNKAQSMLKEAMDEIGHDYYEAEGEAAFYGPKLDVQVKT
EEEEECCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEECCCCCEEEEE
AIGKEETLSTVQLDFLLPEKFDLTYVGEDGKPHRPVVIHRGVVSTMERFVAFLIEEHKGA
CCCCHHHHHEEEEEEECCCCCCEEEECCCCCCCCCEEEECHHHHHHHHHHHHHHHHHCCC
LPTWLAPVQFQVIPVSPSVHLDYAKKVQERLQREGLRVEVDTRDEKIGYKIREAQMQKIP
CCCEECCEEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHCC
YMLVVGDQEVENEAVNVRKYGEQKSETVAIADFIKKAVAEAKK
EEEEECCCHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDMVKIKFPDGAVKEFAKATTTEDIAASISPGLKKKAIAGKLNGKEIDLRAPILEDGAI
CCCEEEECCCHHHHHHHHHCCHHHHHHHCCCCCHHHHHHCCCCCCEEEEECCCCCCCCE
EIITEGSEEALEIMRHSTAHLMAQAIKRLYKNVKLGVGPVIENGFYYDVDLEEALTPEDL
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCEEEEECHHHCCCCCCH
PKIEKEMKKIVGENLPIVRKEVSREEARALFAEIGDDLKLELLDAIPEGETVSIYEQGEF
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEHHHHCCCCCCEEEEEECCCH
FDLCRGVHVPSTGKIKEFKLLSLAGAYWRGDSNNKMLQRIYGTAFFKKAELEEHLRLLEE
HHHHCCCCCCCCCCCHHEEHHHHHCHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKERDHRKLGKELKLFANSQKVGQGLPLWLPKGATIRRVIERYIVDKELALGYEHVYTPV
HHHHHHHHHHHHHHHHCCCHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHCCHHHHHCCC
LGSKDLYETSGHWDHYQDGMFPPMEMDNETLVLRPMNCPHHMMIYKNDIHSYRELPIRIA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEECHHHHHHHCCEEEE
ELGTMHRYEMSGALSGLQRVRGMTLNDAHIFVRPDQIKDEFIRTVRLIQDVYEDFGLEEY
HHCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCEE
SFRLSYRDPEDTEKYYDDDEMWNKAQSMLKEAMDEIGHDYYEAEGEAAFYGPKLDVQVKT
EEEEECCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEECCCCCEEEEE
AIGKEETLSTVQLDFLLPEKFDLTYVGEDGKPHRPVVIHRGVVSTMERFVAFLIEEHKGA
CCCCHHHHHEEEEEEECCCCCCEEEECCCCCCCCCEEEECHHHHHHHHHHHHHHHHHCCC
LPTWLAPVQFQVIPVSPSVHLDYAKKVQERLQREGLRVEVDTRDEKIGYKIREAQMQKIP
CCCEECCEEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHCC
YMLVVGDQEVENEAVNVRKYGEQKSETVAIADFIKKAVAEAKK
EEEEECCCHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2115870; 8969504; 9387221; 9384377; 3027671; 1379177 [H]