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The map label for this gene is lysC [H]

Identifier: 52786727

GI number: 52786727

Start: 2876672

End: 2877901

Strand: Reverse

Name: lysC [H]

Synonym: BLi02996

Alternate gene names: 52786727

Gene position: 2877901-2876672 (Counterclockwise)

Preceding gene: 52786728

Following gene: 52786725

Centisome position: 68.15

GC content: 50.49

Gene sequence:

>1230_bases
ATGGGTCTCATCGTCCAAAAATTTGGCGGAACTTCCGTAGGGTCAGTGGAAAAAATATTGAATGTCGCATACCGCGTCAT
CGAAGAAAAACAGCGCGGTCATGATGTTGTCGTCGTCGTTTCGGCGATGGGCAAATCGACGGACTCCCTCGTCGCGCTTG
CCAAGGAAATCACCGAGCAGCCGAGCAGCAGGGAAATGGACATGCTGCTGACAACAGGAGAGCAGGTGACGATTTCGCTG
TTGACAATGGCTTTGCAGAACAAGGGGTATGATGCGGTTTCCTATACAGGCTGGCAGGCCGGCATCGAAACCGAAAATAT
TCACGGCAACGCAAGAATTACGAACATTGATACAGCCAAGCTGAAAGAGCGCCTGAATGAAGGGAAAATCGCCGTTGTCG
CCGGATTCCAGGGCGTCACAGCGGAAGGCGAAATCACAACGCTCGGCAGAGGCGGATCAGATACGACAGCCGTTGCCCTC
GCAGCAGCCCTGAAAGCTGATAAATGCGACATTTACACCGATGTGCCGGGGGTTTTCACGACGGATCCCCGCTATGTGAA
AACAGCGCGGAAGCTGGCCGGAATCTCCTACGATGAAATGCTGGAGCTTGCCAATCTTGGAGCAGGCGTCCTTCATCCGA
GAGCTGTTGAATTTGCGAAAAACTACCAGGTGCCTTTGGAAGTCCGTTCAAGCACCGAAAAAGAAGCCGGCACATTAATC
GAGGAGGAATCATCAATGGAACAGAATTTAATCGTAAGAGGCATTGCATTTGAAGATCAGATCACACGCGTGACCGTATG
CGGCCTGGCGAGCGGCCTGACGACGCTGTCAACGATTTTTACAACGCTCGCCAAACAGAACATCAACGTTGACATCATCA
TTCAATCGGTGACAGGCACAAGCAAAACGTCGATTTCTTTCTCAGTGAAAACGGAAGACTTAAAACGGACGGTTGAAGTT
CTCGAAGAATACAAAGACGCGCTCGACTATGAACAGATTGAAACGGAAAACAGGCTTGCCAAAGTGTCGATCGTCGGTTC
TGGCATGATCTCCAACCCGGGAGTAGCAGCGGAAATGTTCGCTGTGCTGGCGGAAAAAAACATCCAGGTCAAAATGGTCA
GCACATCCGAAATCAAAGTTTCGACTGTCGTCAACGAAGGCGATATGGTGAAAGCCGTTGAAGCGCTGCATGATGCTTTT
GAACTGTCGAAGCAGACAGCAGTCATTTAA

Upstream 100 bases:

>100_bases
CGTTGCTTTTTTTGTGCAAAAAAAACCGTACAGGCCGCCGGTCAAGCTTTCCTCTTCATACATAAACAAACACGAACGAA
AACAAAAAGGGTGGTATTTG

Downstream 100 bases:

>100_bases
AAAGAAAAAGGCGGGACTTTTGCAGTCTCCGCCTTTTTGCATAGTTATGCCGGGTCTTTCAAATCCCATTTCACCGTTAA
AACCACTTTTCCGGCTCTTT

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase 2; Aspartokinase II [H]

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MGLIVQKFGGTSVGSVEKILNVAYRVIEEKQRGHDVVVVVSAMGKSTDSLVALAKEITEQPSSREMDMLLTTGEQVTISL
LTMALQNKGYDAVSYTGWQAGIETENIHGNARITNIDTAKLKERLNEGKIAVVAGFQGVTAEGEITTLGRGGSDTTAVAL
AAALKADKCDIYTDVPGVFTTDPRYVKTARKLAGISYDEMLELANLGAGVLHPRAVEFAKNYQVPLEVRSSTEKEAGTLI
EEESSMEQNLIVRGIAFEDQITRVTVCGLASGLTTLSTIFTTLAKQNINVDIIIQSVTGTSKTSISFSVKTEDLKRTVEV
LEEYKDALDYEQIETENRLAKVSIVGSGMISNPGVAAEMFAVLAEKNIQVKMVSTSEIKVSTVVNEGDMVKAVEALHDAF
ELSKQTAVI

Sequences:

>Translated_409_residues
MGLIVQKFGGTSVGSVEKILNVAYRVIEEKQRGHDVVVVVSAMGKSTDSLVALAKEITEQPSSREMDMLLTTGEQVTISL
LTMALQNKGYDAVSYTGWQAGIETENIHGNARITNIDTAKLKERLNEGKIAVVAGFQGVTAEGEITTLGRGGSDTTAVAL
AAALKADKCDIYTDVPGVFTTDPRYVKTARKLAGISYDEMLELANLGAGVLHPRAVEFAKNYQVPLEVRSSTEKEAGTLI
EEESSMEQNLIVRGIAFEDQITRVTVCGLASGLTTLSTIFTTLAKQNINVDIIIQSVTGTSKTSISFSVKTEDLKRTVEV
LEEYKDALDYEQIETENRLAKVSIVGSGMISNPGVAAEMFAVLAEKNIQVKMVSTSEIKVSTVVNEGDMVKAVEALHDAF
ELSKQTAVI
>Mature_408_residues
GLIVQKFGGTSVGSVEKILNVAYRVIEEKQRGHDVVVVVSAMGKSTDSLVALAKEITEQPSSREMDMLLTTGEQVTISLL
TMALQNKGYDAVSYTGWQAGIETENIHGNARITNIDTAKLKERLNEGKIAVVAGFQGVTAEGEITTLGRGGSDTTAVALA
AALKADKCDIYTDVPGVFTTDPRYVKTARKLAGISYDEMLELANLGAGVLHPRAVEFAKNYQVPLEVRSSTEKEAGTLIE
EESSMEQNLIVRGIAFEDQITRVTVCGLASGLTTLSTIFTTLAKQNINVDIIIQSVTGTSKTSISFSVKTEDLKRTVEVL
EEYKDALDYEQIETENRLAKVSIVGSGMISNPGVAAEMFAVLAEKNIQVKMVSTSEIKVSTVVNEGDMVKAVEALHDAFE
LSKQTAVI

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=373, Percent_Identity=32.4396782841823, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1790455, Length=287, Percent_Identity=35.5400696864112, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1790376, Length=293, Percent_Identity=25.5972696245734, Blast_Score=82, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6320893, Length=371, Percent_Identity=28.0323450134771, Blast_Score=122, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 43995; Mature: 43864

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLIVQKFGGTSVGSVEKILNVAYRVIEEKQRGHDVVVVVSAMGKSTDSLVALAKEITEQ
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHCC
PSSREMDMLLTTGEQVTISLLTMALQNKGYDAVSYTGWQAGIETENIHGNARITNIDTAK
CCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCEEEECCCHHH
LKERLNEGKIAVVAGFQGVTAEGEITTLGRGGSDTTAVALAAALKADKCDIYTDVPGVFT
HHHHHCCCCEEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCEEECCCCCEE
TDPRYVKTARKLAGISYDEMLELANLGAGVLHPRAVEFAKNYQVPLEVRSSTEKEAGTLI
CCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEECCCCCHHHCCHH
EEESSMEQNLIVRGIAFEDQITRVTVCGLASGLTTLSTIFTTLAKQNINVDIIIQSVTGT
HCHHHHCCCCEEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
SKTSISFSVKTEDLKRTVEVLEEYKDALDYEQIETENRLAKVSIVGSGMISNPGVAAEMF
CCCEEEEEEEHHHHHHHHHHHHHHHHHCCHHHHHCCCCEEEEEEEECCCCCCCCHHHHHH
AVLAEKNIQVKMVSTSEIKVSTVVNEGDMVKAVEALHDAFELSKQTAVI
HHHHCCCEEEEEEECCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GLIVQKFGGTSVGSVEKILNVAYRVIEEKQRGHDVVVVVSAMGKSTDSLVALAKEITEQ
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHCC
PSSREMDMLLTTGEQVTISLLTMALQNKGYDAVSYTGWQAGIETENIHGNARITNIDTAK
CCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCEEEECCCHHH
LKERLNEGKIAVVAGFQGVTAEGEITTLGRGGSDTTAVALAAALKADKCDIYTDVPGVFT
HHHHHCCCCEEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCEEECCCCCEE
TDPRYVKTARKLAGISYDEMLELANLGAGVLHPRAVEFAKNYQVPLEVRSSTEKEAGTLI
CCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEECCCCCHHHCCHH
EEESSMEQNLIVRGIAFEDQITRVTVCGLASGLTTLSTIFTTLAKQNINVDIIIQSVTGT
HCHHHHCCCCEEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
SKTSISFSVKTEDLKRTVEVLEEYKDALDYEQIETENRLAKVSIVGSGMISNPGVAAEMF
CCCEEEEEEEHHHHHHHHHHHHHHHHHCCHHHHHCCCCEEEEEEEECCCCCCCCHHHHHH
AVLAEKNIQVKMVSTSEIKVSTVVNEGDMVKAVEALHDAFELSKQTAVI
HHHHCCCEEEEEEECCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036830; 8969504; 9384377; 2837491; 2168395 [H]