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The map label for this gene is secDF [H]

Identifier: 52786623

GI number: 52786623

Start: 2781772

End: 2783997

Strand: Reverse

Name: secDF [H]

Synonym: BLi02891

Alternate gene names: 52786623

Gene position: 2783997-2781772 (Counterclockwise)

Preceding gene: 52786624

Following gene: 52786622

Centisome position: 65.93

GC content: 47.75

Gene sequence:

>2226_bases
ATGAAAAAAGGACGCTTGATTGCGTTTTTCCTTACCGTTCTCTTGATCGGTACAGGACTGGGAATGTTCACGAAGCCGGC
TGCAGACAACATCAGCCTCGGCCTTGATCTTCAGGGCGGTTTTGAGGTTCTGTATGAAGTCCAGCCTGCCAAACAGGGCG
ACAAAATTGACAGGGATGCCCTCATCAGCACGGTTGAAGCGCTGAACCGCCGGGCGAATGTATTAGGCGTCAGCGAGCCG
AACATTCAAATTGAAGGAAACAACCGGATCAGGGTTCAGCTTGCCGGCGTCGACAACCAGAACAGGGCGCGGGAAATCTT
ATCGACACAGGCTCAACTTACGTTCAGGGATACGAATGACAAAGAGCTTTTGAACGGTGCGGATCTTGTCGAAAACGGCG
CCAAACAGTCGTTCAAGCAGGATACGAATGAGCCGATCGTGACGATCAAGCTGAAGGATGCCGATAAATTCGGCGAAGTG
ACGAAGAAAGTGCTCGATATGAAGCCGAACAACCAGCTCGTCATCTGGCTTGATTACAAAAAAGGCGACTCTTTTAAAAA
GGAATCGCAAAAAGCGGATCATAAATACATTTCCGCTCCGAATGTCAGCCAGGTGCTGAATACGTCAGACGTGATGATTG
AAGGCAACTTTACGATTCAGGAAGCAAAAGACATGGCGAGCATTTTAAATGCGGGAGCTCTTCCTGTCAAACTCGTTGAA
AAGTATTCGACATCCGTCGGAGCGCAGTTTGGGGAGCAGGCTTTGAATGATACCGTGTTTGCGGGAATCATTGGTATTGC
TATTATTTTCTTATTTATGCTTCTTTACTATCGCTTGCCTGGTTTTATTGCGGTCATTACGCTTTCGGTCTACATCTACA
TTACACTGCAGATATTCGACTGGATGAATGCGGTTCTGACCCTTCCGGGGATCGCGGCTCTTATATTGGGCGTCGGGATG
GCGGTTGACGCCAACATCATTACGTATGAACGCATAAAAGAAGAGCTGAAGCTCGGCAAATCGGTCCGTTCGGCTTTTAA
AGCAGGAAACAGAAGATCGTTTGCAACGATTTTTGATGCCAATATTACGACGATGCTTGCCGGAATCGTGCTTTTCATAT
TTGGTACAAGCTCTGTCAAAGGCTTCGCGACCATGCTGATCCTATCCATTTTGACAAGCTTTATTACAGCTGTCTTCCTG
TCGCGCTTCCTGCTCGGCCTGCTGGTCGAAAGCCGCTGGCTTGACCGGAAAAAAGGCTGGTTTGGGGTGCGCAAAAAAGA
CATTTTGGATATCAGAAAGACAGACGAAAATACGGAGCCTCCGAACCCGTTTTCCAAATGGGATTTCGTCGGGAAGCGCA
AATGGTTCTTTGCATTTTCGGGCGTTCTTCTGGCCGCTGGTCTTATCGTCTTGCTTGTGTTTAAGCTGAACCTCGGCATC
GATTTCTCAAGCGGATCGAGAATCGAAGTTCAAAGCGATCATAAGCTGACAACCCAGCAGCTCGAAAAGGACTTTGAACA
AGTCGGGCTTGATCCTGACTCGATCGTTCTTTCAGGCGAAAAAAATGATCATGGCGTCGCCAGGTTTGTCGGCGTGCCGA
ATCAGAAAAAGATCGCTGAAGTGAAAGATTATTTTAAAGACAAATACGGAAGCGAACCAAATGTCAGCACCGTATCGCCT
ACCGTCGGCAAGGAGCTGGCGAGAAATGCGCTTTATGCGGTGATCATCGCGTCAATCGGGATCATTTTGTATGTCTCGAT
CCGCTTTGAATACAAGATGGCGATTGCGGCTATTACTTCATTGCTGTATGACGCGTTTTTCATCATTGCCGTCTTCAGCC
TGACAAGGCTTGAGGTTGATGTTACTTTCATTGCTGCCGTGCTGACGATCATCGGCTATTCGATAAATGATACAATTGTA
ACGTTTGACAGGATCAGGGAGCATATGAAAAAGCGCAAACCGAAAACCTTCAGCGACTTGTCGCATATTGTGAATTTGAG
CTTGCAGCAGACGTTCACGCGTTCGATTAATACGGTGCTGACTGTCGTCATTGTCGTCATTGCACTTCTCATCTTCGGCG
CTGCATCGATTTCGAACTTCTCTGTTGCGCTGTTGGTCGGACTTTTGAGCGGAGTATATTCATCACTCTACATCGCAGCT
CAACTATGGCTTGTCTGGAAAGGGCGCGAACTGAAAAAGCCGAAGCGCAAGCCTGCAGAAGAATAA

Upstream 100 bases:

>100_bases
TAAAACGTCTAATTTACTCGACAGCAAAGAAGGGCAGGATATGCTCGATGAGCTGCTGAGGTAGCCGTTGATTTTGGTTG
ATTTAAGGAGGAAATACATA

Downstream 100 bases:

>100_bases
ATAATGGACTGCCCGCACTGCCGGGCGGTCTTTTTTTGATTTGAATAGAAAAAAAGTTGTTTTGGCGGCTTTCTATCGAT
CTTCTTTTTGTTTAAAATTG

Product: bifunctional preprotein translocase subunit SecD/SecF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 741; Mature: 741

Protein sequence:

>741_residues
MKKGRLIAFFLTVLLIGTGLGMFTKPAADNISLGLDLQGGFEVLYEVQPAKQGDKIDRDALISTVEALNRRANVLGVSEP
NIQIEGNNRIRVQLAGVDNQNRAREILSTQAQLTFRDTNDKELLNGADLVENGAKQSFKQDTNEPIVTIKLKDADKFGEV
TKKVLDMKPNNQLVIWLDYKKGDSFKKESQKADHKYISAPNVSQVLNTSDVMIEGNFTIQEAKDMASILNAGALPVKLVE
KYSTSVGAQFGEQALNDTVFAGIIGIAIIFLFMLLYYRLPGFIAVITLSVYIYITLQIFDWMNAVLTLPGIAALILGVGM
AVDANIITYERIKEELKLGKSVRSAFKAGNRRSFATIFDANITTMLAGIVLFIFGTSSVKGFATMLILSILTSFITAVFL
SRFLLGLLVESRWLDRKKGWFGVRKKDILDIRKTDENTEPPNPFSKWDFVGKRKWFFAFSGVLLAAGLIVLLVFKLNLGI
DFSSGSRIEVQSDHKLTTQQLEKDFEQVGLDPDSIVLSGEKNDHGVARFVGVPNQKKIAEVKDYFKDKYGSEPNVSTVSP
TVGKELARNALYAVIIASIGIILYVSIRFEYKMAIAAITSLLYDAFFIIAVFSLTRLEVDVTFIAAVLTIIGYSINDTIV
TFDRIREHMKKRKPKTFSDLSHIVNLSLQQTFTRSINTVLTVVIVVIALLIFGAASISNFSVALLVGLLSGVYSSLYIAA
QLWLVWKGRELKKPKRKPAEE

Sequences:

>Translated_741_residues
MKKGRLIAFFLTVLLIGTGLGMFTKPAADNISLGLDLQGGFEVLYEVQPAKQGDKIDRDALISTVEALNRRANVLGVSEP
NIQIEGNNRIRVQLAGVDNQNRAREILSTQAQLTFRDTNDKELLNGADLVENGAKQSFKQDTNEPIVTIKLKDADKFGEV
TKKVLDMKPNNQLVIWLDYKKGDSFKKESQKADHKYISAPNVSQVLNTSDVMIEGNFTIQEAKDMASILNAGALPVKLVE
KYSTSVGAQFGEQALNDTVFAGIIGIAIIFLFMLLYYRLPGFIAVITLSVYIYITLQIFDWMNAVLTLPGIAALILGVGM
AVDANIITYERIKEELKLGKSVRSAFKAGNRRSFATIFDANITTMLAGIVLFIFGTSSVKGFATMLILSILTSFITAVFL
SRFLLGLLVESRWLDRKKGWFGVRKKDILDIRKTDENTEPPNPFSKWDFVGKRKWFFAFSGVLLAAGLIVLLVFKLNLGI
DFSSGSRIEVQSDHKLTTQQLEKDFEQVGLDPDSIVLSGEKNDHGVARFVGVPNQKKIAEVKDYFKDKYGSEPNVSTVSP
TVGKELARNALYAVIIASIGIILYVSIRFEYKMAIAAITSLLYDAFFIIAVFSLTRLEVDVTFIAAVLTIIGYSINDTIV
TFDRIREHMKKRKPKTFSDLSHIVNLSLQQTFTRSINTVLTVVIVVIALLIFGAASISNFSVALLVGLLSGVYSSLYIAA
QLWLVWKGRELKKPKRKPAEE
>Mature_741_residues
MKKGRLIAFFLTVLLIGTGLGMFTKPAADNISLGLDLQGGFEVLYEVQPAKQGDKIDRDALISTVEALNRRANVLGVSEP
NIQIEGNNRIRVQLAGVDNQNRAREILSTQAQLTFRDTNDKELLNGADLVENGAKQSFKQDTNEPIVTIKLKDADKFGEV
TKKVLDMKPNNQLVIWLDYKKGDSFKKESQKADHKYISAPNVSQVLNTSDVMIEGNFTIQEAKDMASILNAGALPVKLVE
KYSTSVGAQFGEQALNDTVFAGIIGIAIIFLFMLLYYRLPGFIAVITLSVYIYITLQIFDWMNAVLTLPGIAALILGVGM
AVDANIITYERIKEELKLGKSVRSAFKAGNRRSFATIFDANITTMLAGIVLFIFGTSSVKGFATMLILSILTSFITAVFL
SRFLLGLLVESRWLDRKKGWFGVRKKDILDIRKTDENTEPPNPFSKWDFVGKRKWFFAFSGVLLAAGLIVLLVFKLNLGI
DFSSGSRIEVQSDHKLTTQQLEKDFEQVGLDPDSIVLSGEKNDHGVARFVGVPNQKKIAEVKDYFKDKYGSEPNVSTVSP
TVGKELARNALYAVIIASIGIILYVSIRFEYKMAIAAITSLLYDAFFIIAVFSLTRLEVDVTFIAAVLTIIGYSINDTIV
TFDRIREHMKKRKPKTFSDLSHIVNLSLQQTFTRSINTVLTVVIVVIALLIFGAASISNFSVALLVGLLSGVYSSLYIAA
QLWLVWKGRELKKPKRKPAEE

Specific function: Required for efficient translocation of secretory pre- proteins under conditions of hypersecretion but is not required for the release of mature proteins from the membrane [H]

COG id: COG0342

COG function: function code U; Preprotein translocase subunit SecD

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the secD/secF family. SecF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786609, Length=390, Percent_Identity=32.3076923076923, Blast_Score=165, Evalue=1e-41,
Organism=Escherichia coli, GI1786610, Length=287, Percent_Identity=29.9651567944251, Blast_Score=151, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005791
- InterPro:   IPR022813
- InterPro:   IPR022646
- InterPro:   IPR022645
- InterPro:   IPR005665 [H]

Pfam domain/function: PF07549 Sec_GG; PF02355 SecD_SecF [H]

EC number: NA

Molecular weight: Translated: 82176; Mature: 82176

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKGRLIAFFLTVLLIGTGLGMFTKPAADNISLGLDLQGGFEVLYEVQPAKQGDKIDRDA
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCHHH
LISTVEALNRRANVLGVSEPNIQIEGNNRIRVQLAGVDNQNRAREILSTQAQLTFRDTND
HHHHHHHHHHCCCEEECCCCCEEEECCCEEEEEEECCCCHHHHHHHHHCCCEEEEECCCC
KELLNGADLVENGAKQSFKQDTNEPIVTIKLKDADKFGEVTKKVLDMKPNNQLVIWLDYK
HHHHCCHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCEEEEEEEEC
KGDSFKKESQKADHKYISAPNVSQVLNTSDVMIEGNFTIQEAKDMASILNAGALPVKLVE
CCCCHHHHHHHHCCCCCCCCCHHHHHCCCCEEEECCEEHHHHHHHHHHHCCCCCHHHHHH
KYSTSVGAQFGEQALNDTVFAGIIGIAIIFLFMLLYYRLPGFIAVITLSVYIYITLQIFD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WMNAVLTLPGIAALILGVGMAVDANIITYERIKEELKLGKSVRSAFKAGNRRSFATIFDA
HHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECC
NITTMLAGIVLFIFGTSSVKGFATMLILSILTSFITAVFLSRFLLGLLVESRWLDRKKGW
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
FGVRKKDILDIRKTDENTEPPNPFSKWDFVGKRKWFFAFSGVLLAAGLIVLLVFKLNLGI
CCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCE
DFSSGSRIEVQSDHKLTTQQLEKDFEQVGLDPDSIVLSGEKNDHGVARFVGVPNQKKIAE
EECCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEEEECCCCHHHHHH
VKDYFKDKYGSEPNVSTVSPTVGKELARNALYAVIIASIGIILYVSIRFEYKMAIAAITS
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHH
LLYDAFFIIAVFSLTRLEVDVTFIAAVLTIIGYSINDTIVTFDRIREHMKKRKPKTFSDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHCCCCCHHHH
SHIVNLSLQQTFTRSINTVLTVVIVVIALLIFGAASISNFSVALLVGLLSGVYSSLYIAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
QLWLVWKGRELKKPKRKPAEE
HHHHEECCCHHCCCCCCCCCC
>Mature Secondary Structure
MKKGRLIAFFLTVLLIGTGLGMFTKPAADNISLGLDLQGGFEVLYEVQPAKQGDKIDRDA
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCHHH
LISTVEALNRRANVLGVSEPNIQIEGNNRIRVQLAGVDNQNRAREILSTQAQLTFRDTND
HHHHHHHHHHCCCEEECCCCCEEEECCCEEEEEEECCCCHHHHHHHHHCCCEEEEECCCC
KELLNGADLVENGAKQSFKQDTNEPIVTIKLKDADKFGEVTKKVLDMKPNNQLVIWLDYK
HHHHCCHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCEEEEEEEEC
KGDSFKKESQKADHKYISAPNVSQVLNTSDVMIEGNFTIQEAKDMASILNAGALPVKLVE
CCCCHHHHHHHHCCCCCCCCCHHHHHCCCCEEEECCEEHHHHHHHHHHHCCCCCHHHHHH
KYSTSVGAQFGEQALNDTVFAGIIGIAIIFLFMLLYYRLPGFIAVITLSVYIYITLQIFD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WMNAVLTLPGIAALILGVGMAVDANIITYERIKEELKLGKSVRSAFKAGNRRSFATIFDA
HHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECC
NITTMLAGIVLFIFGTSSVKGFATMLILSILTSFITAVFLSRFLLGLLVESRWLDRKKGW
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
FGVRKKDILDIRKTDENTEPPNPFSKWDFVGKRKWFFAFSGVLLAAGLIVLLVFKLNLGI
CCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCE
DFSSGSRIEVQSDHKLTTQQLEKDFEQVGLDPDSIVLSGEKNDHGVARFVGVPNQKKIAE
EECCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEEEECCCCHHHHHH
VKDYFKDKYGSEPNVSTVSPTVGKELARNALYAVIIASIGIILYVSIRFEYKMAIAAITS
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHH
LLYDAFFIIAVFSLTRLEVDVTFIAAVLTIIGYSINDTIVTFDRIREHMKKRKPKTFSDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHCCCCCHHHH
SHIVNLSLQQTFTRSINTVLTVVIVVIALLIFGAASISNFSVALLVGLLSGVYSSLYIAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
QLWLVWKGRELKKPKRKPAEE
HHHHEECCCHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9694879; 9384377 [H]