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The map label for this gene is ispG [H]

Identifier: 52786419

GI number: 52786419

Start: 2586379

End: 2587491

Strand: Direct

Name: ispG [H]

Synonym: BLi02683

Alternate gene names: 52786419

Gene position: 2586379-2587491 (Clockwise)

Preceding gene: 52786418

Following gene: 52786421

Centisome position: 61.25

GC content: 50.67

Gene sequence:

>1113_bases
GTGAGTGAAATCACACATCGTACAAAAACGCGTCCCGTTAAAGTGGGACCTTTAACAATAGGCGGCAATAACGAAGTCGT
CATTCAAAGCATGACAACAACGAAAACACATGACGTTGAAGCAACCGTCGCCGAAATCAACAGACTCGCGGAAGCAGGAT
GTCAAATCGTCCGCGTCGCCTGTCCTGATGAACGGGCTGCCGACGCCATTCCAGAGATCAAAAAGCGGATATCCATCCCT
CTTGTCGTGGATATTCATTTCAACTATAAATTGGCATTAAAAGCGATCGAAGGCGGAGCCGATAAAATCCGCATCAATCC
GGGTAACATCGGCCGCCGCGAAAAGGTTGAAGCGGTCGTCAACGCAGCGAAGGAAAAGGGCATTCCGATCCGGATCGGCG
TCAATGCAGGCTCTCTGGAAAAACGAATCCTTGAGAAGTACGGCTATCCGACAGCAGACGGCATGGTCGAAAGCGCCCTG
CACCACATTAAAATTCTTGAGGATCTCGATTTCCACGATATCATCGTCAGCATGAAGGCGTCTGATGTAAACCTGGCGAT
TGAGGCATATGAAAAAGCGGCTAAAGCTTTCGATTATCCGCTTCATTTAGGCATCACCGAATCGGGAACGCTGTTTGCCG
GTACAGTGAAAAGCGCCGCGGGTCTCGGCGCGATCCTTTCAAAAGGAATCGGCAATACATTGCGGATTTCTTTAAGCGCG
GACCCGGTCGAAGAAGTAAAAGTCGCCAGGGAGCTCCTGAAATCTTTCGGGCTCGCTTCCAATGCGGCAACATTGATTTC
CTGCCCGACTTGCGGCCGGATCGAAATCGATTTGATCAGCATTGCCAATGAAGTCGAAGATTACATCGCGAAAATCAAGG
CGCCGATCAAAGTTGCCGTTCTCGGCTGTGCCGTCAACGGTCCCGGAGAAGCGCGCGAAGCCGATATCGGGATCGCCGGC
GCACGCGGCGAAGGACTTCTGTTCCGCAAAGGCGAAATCGTCCGGAAAGTGCCTGAAGAAACGATGGTCGAGGAATTGAA
AAAGGAAATCGACAAGCTCGCCGAAGAGCATTATGCCAAACAAGCGGCAGAAAAAGAAAAATTAAACACATAA

Upstream 100 bases:

>100_bases
TGTCAGTTTCATGAACGGGCTGTTACAATAAGACTTGTAAACGATTTGGTATAAGAAAAGGAGCAACCGCCTCCGAATTA
TACTTAAGGAGCGAATTCAA

Downstream 100 bases:

>100_bases
TAAAAAAAAGCTTGACGGATATCCGTCAAGCTTTTTCCGTTAGAAAAACGGGGTGATGAATATGCCAAGCACCAAGGAAA
CTGCACCGATACCCATTGCC

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 370; Mature: 369

Protein sequence:

>370_residues
MSEITHRTKTRPVKVGPLTIGGNNEVVIQSMTTTKTHDVEATVAEINRLAEAGCQIVRVACPDERAADAIPEIKKRISIP
LVVDIHFNYKLALKAIEGGADKIRINPGNIGRREKVEAVVNAAKEKGIPIRIGVNAGSLEKRILEKYGYPTADGMVESAL
HHIKILEDLDFHDIIVSMKASDVNLAIEAYEKAAKAFDYPLHLGITESGTLFAGTVKSAAGLGAILSKGIGNTLRISLSA
DPVEEVKVARELLKSFGLASNAATLISCPTCGRIEIDLISIANEVEDYIAKIKAPIKVAVLGCAVNGPGEAREADIGIAG
ARGEGLLFRKGEIVRKVPEETMVEELKKEIDKLAEEHYAKQAAEKEKLNT

Sequences:

>Translated_370_residues
MSEITHRTKTRPVKVGPLTIGGNNEVVIQSMTTTKTHDVEATVAEINRLAEAGCQIVRVACPDERAADAIPEIKKRISIP
LVVDIHFNYKLALKAIEGGADKIRINPGNIGRREKVEAVVNAAKEKGIPIRIGVNAGSLEKRILEKYGYPTADGMVESAL
HHIKILEDLDFHDIIVSMKASDVNLAIEAYEKAAKAFDYPLHLGITESGTLFAGTVKSAAGLGAILSKGIGNTLRISLSA
DPVEEVKVARELLKSFGLASNAATLISCPTCGRIEIDLISIANEVEDYIAKIKAPIKVAVLGCAVNGPGEAREADIGIAG
ARGEGLLFRKGEIVRKVPEETMVEELKKEIDKLAEEHYAKQAAEKEKLNT
>Mature_369_residues
SEITHRTKTRPVKVGPLTIGGNNEVVIQSMTTTKTHDVEATVAEINRLAEAGCQIVRVACPDERAADAIPEIKKRISIPL
VVDIHFNYKLALKAIEGGADKIRINPGNIGRREKVEAVVNAAKEKGIPIRIGVNAGSLEKRILEKYGYPTADGMVESALH
HIKILEDLDFHDIIVSMKASDVNLAIEAYEKAAKAFDYPLHLGITESGTLFAGTVKSAAGLGAILSKGIGNTLRISLSAD
PVEEVKVARELLKSFGLASNAATLISCPTCGRIEIDLISIANEVEDYIAKIKAPIKVAVLGCAVNGPGEAREADIGIAGA
RGEGLLFRKGEIVRKVPEETMVEELKKEIDKLAEEHYAKQAAEKEKLNT

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=345, Percent_Identity=48.695652173913, Blast_Score=336, Evalue=1e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 39779; Mature: 39648

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEITHRTKTRPVKVGPLTIGGNNEVVIQSMTTTKTHDVEATVAEINRLAEAGCQIVRVA
CCCCCCCCCCCCEEECCEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEE
CPDERAADAIPEIKKRISIPLVVDIHFNYKLALKAIEGGADKIRINPGNIGRREKVEAVV
CCCCHHHHHHHHHHHHCCCCEEEEEECCEEEEEEEECCCCCEEEECCCCCCHHHHHHHHH
NAAKEKGIPIRIGVNAGSLEKRILEKYGYPTADGMVESALHHIKILEDLDFHDIIVSMKA
HHHHHCCCEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHEEEEEEC
SDVNLAIEAYEKAAKAFDYPLHLGITESGTLFAGTVKSAAGLGAILSKGIGNTLRISLSA
CCCEEHHHHHHHHHHHCCCCEEEEECCCCCEEEEHHHHHHHHHHHHHCCCCCEEEEEECC
DPVEEVKVARELLKSFGLASNAATLISCPTCGRIEIDLISIANEVEDYIAKIKAPIKVAV
CCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCEEEEHHHHHHHHHHHHHHHCCCEEEEE
LGCAVNGPGEAREADIGIAGARGEGLLFRKGEIVRKVPEETMVEELKKEIDKLAEEHYAK
EEEEECCCCCCCCCCCEEECCCCCCEEEECCHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
QAAEKEKLNT
HHHHHHCCCC
>Mature Secondary Structure 
SEITHRTKTRPVKVGPLTIGGNNEVVIQSMTTTKTHDVEATVAEINRLAEAGCQIVRVA
CCCCCCCCCCCEEECCEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEE
CPDERAADAIPEIKKRISIPLVVDIHFNYKLALKAIEGGADKIRINPGNIGRREKVEAVV
CCCCHHHHHHHHHHHHCCCCEEEEEECCEEEEEEEECCCCCEEEECCCCCCHHHHHHHHH
NAAKEKGIPIRIGVNAGSLEKRILEKYGYPTADGMVESALHHIKILEDLDFHDIIVSMKA
HHHHHCCCEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHEEEEEEC
SDVNLAIEAYEKAAKAFDYPLHLGITESGTLFAGTVKSAAGLGAILSKGIGNTLRISLSA
CCCEEHHHHHHHHHHHCCCCEEEEECCCCCEEEEHHHHHHHHHHHHHCCCCCEEEEEECC
DPVEEVKVARELLKSFGLASNAATLISCPTCGRIEIDLISIANEVEDYIAKIKAPIKVAV
CCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCEEEEHHHHHHHHHHHHHHHCCCEEEEE
LGCAVNGPGEAREADIGIAGARGEGLLFRKGEIVRKVPEETMVEELKKEIDKLAEEHYAK
EEEEECCCCCCCCCCCEEECCCCCCEEEECCHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
QAAEKEKLNT
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]