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The map label for this gene is bkdR [H]

Identifier: 52786325

GI number: 52786325

Start: 2505855

End: 2507924

Strand: Reverse

Name: bkdR [H]

Synonym: BLi02587

Alternate gene names: 52786325

Gene position: 2507924-2505855 (Counterclockwise)

Preceding gene: 52786327

Following gene: 52786324

Centisome position: 59.39

GC content: 48.6

Gene sequence:

>2070_bases
ATGATGCAAAAAGTACTGATCGTTGGTGCTGGAAAAGGGGGGACCGCCCTCTTGGATTTGCTGCTGAAAACAAAGACGAT
GCATATCGAGGCGGTCATCGATAAAAATCCGGAAGCTCCCGGGCTTTTCGTCGCGCAGAAAAACGATATCGAAACGGCAC
TCGACTGGACGCCATACATCACAGAACAGATCGACATCATCATTGAGACGACCGGAGACGCCGGCGTGTTAAAGCAGCTG
ATGCGAAAAAAGCACGACAGAACGATCGTGGTGCCGGGATCGCTCGCTTATATCATCTCTCAGCTGATGAATGAAAAGCA
GCAGCTCATCCAAATGTTAAAAGAGCAGACGTATAAACACGACCGTATTTTCAACTCGATGAATGACGGCATGATTTTTA
TCGACATTAACGAAGAGATCATTTTGTTTAACAAAATGGCGGAGGTTATGACCGGAACAAAGCGCAGCGAAGCGATCGGC
CAGCATATTCAAAGCGTGATCCCGACGACGAAGCTGCCGCGTATTCTCAACACGAGAGAGCCTGAGTATCATCAAAAACA
ATTTCTCCATCCGAACAGGCAGATTGTCACGACCCGAATTCCGATTATTGATGACGGAGGAACGCTCCTCGGGGCGCTCA
GCATATTTAAGGACATTACCGATGCGGTCGAGCTGGCTGAAGAGGTGACCAACCTGAAGGAAGTCAGAACGATGCTTGAA
GCGATCATTCAATCTTCCGATGAAGCGATTTCGGTCGTTGACGAAAACGGAAACGGAATGATGATCAACAGGGCATATAC
GAAGATGACAGGTCTGACAAAAGACCAGGTGATCGGGAAGCCGGCTAATACCGACATATCTGAAGGCGAGAGCATGCATT
TGAAAGTGCTCGAAACGAGGCGCCCTGTCAGAGGGGTCAGAATGAAAGTCGGCCCGAATAAAAAAGAAGTGATCGTCAAT
GTTGCGCCGATTATCGTCGACGGCATTTTAAAAGGGAGCGTCGGCGTCATTCACGACGTATCTGAAATTCAATCGCTCAC
AAACGAGCTGAACAGAGCACGGCAGATTATCCGTACACTTGAGGCGAAATATACATTTGCCGACATCATCGGGTCAAGCG
AGCAGATGCTCGTCGCGCTGGAACAGGCGAAATTGGGGGCGAAGACGCCTGCGACGATTTTGCTGAGAGGCGAATCGGGA
ACGGGTAAAGAGCTTTTTGCCCATGCCATACATAACGAAAGTGACCGAAAGTATAATAAGTTTGTCCGCGTCAATTGTGC
GGCCATATCGGAATCACTGCTTGAATCAGAGCTTTTCGGCTATGAGGAAGGCGCTTTTTCAGGTGCGCGCCGCGGCGGGA
AAAAAGGATTCTTTGAAGAAGCGAACAACGGAAGCATTTTCTTGGATGAAATCGGCGAGCTCTCGCTAAACACACAGGCG
AAGCTCCTCCGCGTTCTTCAGGAAAAAGAAATCGTCAGAGTCGGCGGGACCAAACCGATCCCTGTGAACGTCCGGGTCAT
CGCGGCGACTAACGTCAATATTGAAAAGGCGCTCGCTGAAGGCCGGTTTCGCGAAGACTTGTACTACCGGATCAATCGCT
ATCCGATATCGATTCCGCCGCTCAGACAGCGGAAAGAGGACATTGAAGCCTTGAGCAGGCATTTGATAGGGAAGATCAAC
CAAGAGTACGGGCGCAATGTAAAGGGGCTTACGAAAAACGCCCTCAAATCGCTAAAAGCGCGGAAGTGGCCGGGGAATGT
CAGAGAGCTTGAAAATGTCCTCGAGCGGGCGATGATTTTTTTAAAACCGCAAATGGAATGGATTGATCTAGAACATCTTC
CTGAAGCGGACCGTCCGAGAAAAAAGGTGGAAGCCGGCGAAGCTTTCCCTGAAATTGAAGATGAAAAGCTGTCTGATGCG
GTCGAACGGTTTGAAGCTCATATCATTAAAGAAACGCTTGAAAAGCATCAATATAACAGAACGAAAACGGCAAAAGCTCT
TGGAATCAGCATTCGCAACCTCTATTACAAAATGGATAAATACAACCTTGCAAAAGATAGCATGCAATAA

Upstream 100 bases:

>100_bases
AGTTTGTCAAGAAAGGAAAACATGATAACATAGATGTTGTGCAAATTGTTGCAAGAGAACGTGAGAGTGTGCAAAATTTT
TCATACAAAGGGGTAAAGGG

Downstream 100 bases:

>100_bases
ATTGCAAAAACGAGTGCAAAATCATGCAAACTGTAAACAGGTTCACAGTACCGAACCGCTTTTTTCATACTGGCACGAAA
CTTGCATGATATAGTGGGCG

Product: BkdR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 689; Mature: 689

Protein sequence:

>689_residues
MMQKVLIVGAGKGGTALLDLLLKTKTMHIEAVIDKNPEAPGLFVAQKNDIETALDWTPYITEQIDIIIETTGDAGVLKQL
MRKKHDRTIVVPGSLAYIISQLMNEKQQLIQMLKEQTYKHDRIFNSMNDGMIFIDINEEIILFNKMAEVMTGTKRSEAIG
QHIQSVIPTTKLPRILNTREPEYHQKQFLHPNRQIVTTRIPIIDDGGTLLGALSIFKDITDAVELAEEVTNLKEVRTMLE
AIIQSSDEAISVVDENGNGMMINRAYTKMTGLTKDQVIGKPANTDISEGESMHLKVLETRRPVRGVRMKVGPNKKEVIVN
VAPIIVDGILKGSVGVIHDVSEIQSLTNELNRARQIIRTLEAKYTFADIIGSSEQMLVALEQAKLGAKTPATILLRGESG
TGKELFAHAIHNESDRKYNKFVRVNCAAISESLLESELFGYEEGAFSGARRGGKKGFFEEANNGSIFLDEIGELSLNTQA
KLLRVLQEKEIVRVGGTKPIPVNVRVIAATNVNIEKALAEGRFREDLYYRINRYPISIPPLRQRKEDIEALSRHLIGKIN
QEYGRNVKGLTKNALKSLKARKWPGNVRELENVLERAMIFLKPQMEWIDLEHLPEADRPRKKVEAGEAFPEIEDEKLSDA
VERFEAHIIKETLEKHQYNRTKTAKALGISIRNLYYKMDKYNLAKDSMQ

Sequences:

>Translated_689_residues
MMQKVLIVGAGKGGTALLDLLLKTKTMHIEAVIDKNPEAPGLFVAQKNDIETALDWTPYITEQIDIIIETTGDAGVLKQL
MRKKHDRTIVVPGSLAYIISQLMNEKQQLIQMLKEQTYKHDRIFNSMNDGMIFIDINEEIILFNKMAEVMTGTKRSEAIG
QHIQSVIPTTKLPRILNTREPEYHQKQFLHPNRQIVTTRIPIIDDGGTLLGALSIFKDITDAVELAEEVTNLKEVRTMLE
AIIQSSDEAISVVDENGNGMMINRAYTKMTGLTKDQVIGKPANTDISEGESMHLKVLETRRPVRGVRMKVGPNKKEVIVN
VAPIIVDGILKGSVGVIHDVSEIQSLTNELNRARQIIRTLEAKYTFADIIGSSEQMLVALEQAKLGAKTPATILLRGESG
TGKELFAHAIHNESDRKYNKFVRVNCAAISESLLESELFGYEEGAFSGARRGGKKGFFEEANNGSIFLDEIGELSLNTQA
KLLRVLQEKEIVRVGGTKPIPVNVRVIAATNVNIEKALAEGRFREDLYYRINRYPISIPPLRQRKEDIEALSRHLIGKIN
QEYGRNVKGLTKNALKSLKARKWPGNVRELENVLERAMIFLKPQMEWIDLEHLPEADRPRKKVEAGEAFPEIEDEKLSDA
VERFEAHIIKETLEKHQYNRTKTAKALGISIRNLYYKMDKYNLAKDSMQ
>Mature_689_residues
MMQKVLIVGAGKGGTALLDLLLKTKTMHIEAVIDKNPEAPGLFVAQKNDIETALDWTPYITEQIDIIIETTGDAGVLKQL
MRKKHDRTIVVPGSLAYIISQLMNEKQQLIQMLKEQTYKHDRIFNSMNDGMIFIDINEEIILFNKMAEVMTGTKRSEAIG
QHIQSVIPTTKLPRILNTREPEYHQKQFLHPNRQIVTTRIPIIDDGGTLLGALSIFKDITDAVELAEEVTNLKEVRTMLE
AIIQSSDEAISVVDENGNGMMINRAYTKMTGLTKDQVIGKPANTDISEGESMHLKVLETRRPVRGVRMKVGPNKKEVIVN
VAPIIVDGILKGSVGVIHDVSEIQSLTNELNRARQIIRTLEAKYTFADIIGSSEQMLVALEQAKLGAKTPATILLRGESG
TGKELFAHAIHNESDRKYNKFVRVNCAAISESLLESELFGYEEGAFSGARRGGKKGFFEEANNGSIFLDEIGELSLNTQA
KLLRVLQEKEIVRVGGTKPIPVNVRVIAATNVNIEKALAEGRFREDLYYRINRYPISIPPLRQRKEDIEALSRHLIGKIN
QEYGRNVKGLTKNALKSLKARKWPGNVRELENVLERAMIFLKPQMEWIDLEHLPEADRPRKKVEAGEAFPEIEDEKLSDA
VERFEAHIIKETLEKHQYNRTKTAKALGISIRNLYYKMDKYNLAKDSMQ

Specific function: Member Of The Two-Component Regulatory System Atos/Atoc Involved In The Transcriptional Regulation Of The Ato Genes For Acetoacetate Metabolism. Also An Inhibitor Of Polyamine Biosynthesis. [C]

COG id: COG3829

COG function: function code KT; Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=350, Percent_Identity=43.4285714285714, Blast_Score=274, Evalue=1e-74,
Organism=Escherichia coli, GI1789233, Length=464, Percent_Identity=33.1896551724138, Blast_Score=263, Evalue=4e-71,
Organism=Escherichia coli, GI87082117, Length=275, Percent_Identity=45.8181818181818, Blast_Score=248, Evalue=1e-66,
Organism=Escherichia coli, GI1789087, Length=256, Percent_Identity=48.4375, Blast_Score=246, Evalue=3e-66,
Organism=Escherichia coli, GI1786524, Length=403, Percent_Identity=36.4764267990074, Blast_Score=246, Evalue=3e-66,
Organism=Escherichia coli, GI1790437, Length=312, Percent_Identity=43.5897435897436, Blast_Score=243, Evalue=3e-65,
Organism=Escherichia coli, GI1788905, Length=311, Percent_Identity=39.871382636656, Blast_Score=225, Evalue=9e-60,
Organism=Escherichia coli, GI87082152, Length=315, Percent_Identity=40, Blast_Score=218, Evalue=1e-57,
Organism=Escherichia coli, GI1790299, Length=227, Percent_Identity=46.2555066079295, Blast_Score=213, Evalue=5e-56,
Organism=Escherichia coli, GI1787583, Length=325, Percent_Identity=37.5384615384615, Blast_Score=199, Evalue=4e-52,
Organism=Escherichia coli, GI87081872, Length=322, Percent_Identity=34.472049689441, Blast_Score=187, Evalue=3e-48,
Organism=Escherichia coli, GI87081858, Length=532, Percent_Identity=25.187969924812, Blast_Score=151, Evalue=2e-37,
Organism=Escherichia coli, GI1789828, Length=225, Percent_Identity=36.8888888888889, Blast_Score=137, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR002197
- InterPro:   IPR016040
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR002078 [H]

Pfam domain/function: PF02954 HTH_8; PF00989 PAS; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 77577; Mature: 77577

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: PS50112 PAS ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMQKVLIVGAGKGGTALLDLLLKTKTMHIEAVIDKNPEAPGLFVAQKNDIETALDWTPYI
CCCEEEEEECCCCHHHHHHHHHHHHHEEEEEEECCCCCCCEEEEEECCCCCHHHCCCCCC
TEQIDIIIETTGDAGVLKQLMRKKHDRTIVVPGSLAYIISQLMNEKQQLIQMLKEQTYKH
CCEEEEEEEECCCHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRIFNSMNDGMIFIDINEEIILFNKMAEVMTGTKRSEAIGQHIQSVIPTTKLPRILNTRE
HHHHHCCCCCEEEEECCCCEEEHHHHHHHHCCCCHHHHHHHHHHHHCCCHHCCHHHCCCC
PEYHQKQFLHPNRQIVTTRIPIIDDGGTLLGALSIFKDITDAVELAEEVTNLKEVRTMLE
CCHHHHHHCCCCCEEEEEECCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIIQSSDEAISVVDENGNGMMINRAYTKMTGLTKDQVIGKPANTDISEGESMHLKVLETR
HHHCCCCCEEEEEECCCCEEEEEEHHHHHHCCCHHHHCCCCCCCCCCCCCCEEEEEECCC
RPVRGVRMKVGPNKKEVIVNVAPIIVDGILKGSVGVIHDVSEIQSLTNELNRARQIIRTL
CCCCCEEEEECCCCCEEEEEEHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHHH
EAKYTFADIIGSSEQMLVALEQAKLGAKTPATILLRGESGTGKELFAHAIHNESDRKYNK
HHHHHHHHHHCCCCHHHEEEHHHHCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCCCHHH
FVRVNCAAISESLLESELFGYEEGAFSGARRGGKKGFFEEANNGSIFLDEIGELSLNTQA
EEEEEHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCHHH
KLLRVLQEKEIVRVGGTKPIPVNVRVIAATNVNIEKALAEGRFREDLYYRINRYPISIPP
HHHHHHHHCCEEEECCCCCCEEEEEEEEEECCCHHHHHHCCCHHHHHHHEEECCCCCCCC
LRQRKEDIEALSRHLIGKINQEYGRNVKGLTKNALKSLKARKWPGNVRELENVLERAMIF
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
LKPQMEWIDLEHLPEADRPRKKVEAGEAFPEIEDEKLSDAVERFEAHIIKETLEKHQYNR
HCCCCCEECHHHCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCH
TKTAKALGISIRNLYYKMDKYNLAKDSMQ
HHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MMQKVLIVGAGKGGTALLDLLLKTKTMHIEAVIDKNPEAPGLFVAQKNDIETALDWTPYI
CCCEEEEEECCCCHHHHHHHHHHHHHEEEEEEECCCCCCCEEEEEECCCCCHHHCCCCCC
TEQIDIIIETTGDAGVLKQLMRKKHDRTIVVPGSLAYIISQLMNEKQQLIQMLKEQTYKH
CCEEEEEEEECCCHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRIFNSMNDGMIFIDINEEIILFNKMAEVMTGTKRSEAIGQHIQSVIPTTKLPRILNTRE
HHHHHCCCCCEEEEECCCCEEEHHHHHHHHCCCCHHHHHHHHHHHHCCCHHCCHHHCCCC
PEYHQKQFLHPNRQIVTTRIPIIDDGGTLLGALSIFKDITDAVELAEEVTNLKEVRTMLE
CCHHHHHHCCCCCEEEEEECCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIIQSSDEAISVVDENGNGMMINRAYTKMTGLTKDQVIGKPANTDISEGESMHLKVLETR
HHHCCCCCEEEEEECCCCEEEEEEHHHHHHCCCHHHHCCCCCCCCCCCCCCEEEEEECCC
RPVRGVRMKVGPNKKEVIVNVAPIIVDGILKGSVGVIHDVSEIQSLTNELNRARQIIRTL
CCCCCEEEEECCCCCEEEEEEHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHHH
EAKYTFADIIGSSEQMLVALEQAKLGAKTPATILLRGESGTGKELFAHAIHNESDRKYNK
HHHHHHHHHHCCCCHHHEEEHHHHCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCCCHHH
FVRVNCAAISESLLESELFGYEEGAFSGARRGGKKGFFEEANNGSIFLDEIGELSLNTQA
EEEEEHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCHHH
KLLRVLQEKEIVRVGGTKPIPVNVRVIAATNVNIEKALAEGRFREDLYYRINRYPISIPP
HHHHHHHHCCEEEECCCCCCEEEEEEEEEECCCHHHHHHCCCHHHHHHHEEECCCCCCCC
LRQRKEDIEALSRHLIGKINQEYGRNVKGLTKNALKSLKARKWPGNVRELENVLERAMIF
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
LKPQMEWIDLEHLPEADRPRKKVEAGEAFPEIEDEKLSDAVERFEAHIIKETLEKHQYNR
HCCCCCEECHHHCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCH
TKTAKALGISIRNLYYKMDKYNLAKDSMQ
HHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]