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The map label for this gene is gmuC [H]

Identifier: 52786300

GI number: 52786300

Start: 2481226

End: 2482119

Strand: Reverse

Name: gmuC [H]

Synonym: BLi02562

Alternate gene names: 52786300

Gene position: 2482119-2481226 (Counterclockwise)

Preceding gene: 52786301

Following gene: 52786299

Centisome position: 58.78

GC content: 46.87

Gene sequence:

>894_bases
ATGTTTGAAAAATTGAGCAACCTCTTGGTGCCGATTGCCGGGAAGCTGAATAATAACCGGTATTTAGGCGTGCTGAGGGA
TGCATTTATGCTGGCTTTTCCGCTCACCATTTTCGGATCGATCATTGTCGTTTTAACCAATTTGCCGTTTCTCAATAAAA
TGATGAGCAAATCCGCGCTTGAAACGTTCCAGTCGGCTTTAAGCATAGCGCCGAGCGCAACGATCAGCATCATGTCCGTT
TTCGTCGTATTCGGGATCGGGTATTATTTGTCCAAAACTTATGAAGTCGAAGCGGTTTTTGGAGGAGTCGTCGCTTTGGC
ATCGTTCCTTCTGCTGACTCCGTTTGTATTAAACGGCGAAGGAGGGGAAGTGATTCCGAACGTCATCCCGATTGACAGGC
TCGGTGCGAAAGGGATGTTCCTCGGAATGATTACCGCCTTTACATCCGCTGAGATTTTCAGATTTTTTGTCCAGAAAAAC
TATACGATCAAAATGCCTGCCGGCGTTCCGCCTGCTGTAGCAAAATCTTTTGCCGCGCTGATTCCGGCGGTGATGACCTT
GTCCGTCTACTTGGTGATCAATATCCTTGTAACCCAAGCGTTCAATACGAATATGCACGATTTAATTTACAACCTGGTGC
AGGCTCCGCTTGTCGGTCTTGGAAGCGGTATCATTCCGACCTTGATTGCCATATTTTTCACTCAGCTGCTCTGGTTTTTC
GGACTCCACGGACAAATTATCATCAACTCCGTTATGGATCCGATCTGGAATACGCTTTCGATCGAAAATTTGAATAGCTA
TACCGCAACAGGAGAAGTGCCGCATATTATCAATAAGCAGTTTATGGAAGTCTACACCGTCGGAATGGGCGGAACCGGCA
TGACCTTGGCGTGA

Upstream 100 bases:

>100_bases
GCTCACGATGAAGGAACTCGTTTCTGAATTATTGGAATTGTTCAAAGAGAAAAGTCTATGATTCGCACTGCATGGCAAAA
TCGAAGGGGGATAAGAGACC

Downstream 100 bases:

>100_bases
TTATTGCGATCCTGCTGTTTATGAGGAGCAAGCAGATGAAGCAGGTCGCAAAACTCGGAGCAGCCCCGGGAATTTTCAAC
GTAAATGAACCGATCATTTT

Product: hypothetical protein

Products: pyruvate; diacetylchitobiose-6-phosphate [Cytoplasm]; cellobiose-6-phosphate [Cytoplasm] [C]

Alternate protein names: Glucomannan utilization protein C; PTS system oligo-beta-mannoside-specific EIIC component [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MFEKLSNLLVPIAGKLNNNRYLGVLRDAFMLAFPLTIFGSIIVVLTNLPFLNKMMSKSALETFQSALSIAPSATISIMSV
FVVFGIGYYLSKTYEVEAVFGGVVALASFLLLTPFVLNGEGGEVIPNVIPIDRLGAKGMFLGMITAFTSAEIFRFFVQKN
YTIKMPAGVPPAVAKSFAALIPAVMTLSVYLVINILVTQAFNTNMHDLIYNLVQAPLVGLGSGIIPTLIAIFFTQLLWFF
GLHGQIIINSVMDPIWNTLSIENLNSYTATGEVPHIINKQFMEVYTVGMGGTGMTLA

Sequences:

>Translated_297_residues
MFEKLSNLLVPIAGKLNNNRYLGVLRDAFMLAFPLTIFGSIIVVLTNLPFLNKMMSKSALETFQSALSIAPSATISIMSV
FVVFGIGYYLSKTYEVEAVFGGVVALASFLLLTPFVLNGEGGEVIPNVIPIDRLGAKGMFLGMITAFTSAEIFRFFVQKN
YTIKMPAGVPPAVAKSFAALIPAVMTLSVYLVINILVTQAFNTNMHDLIYNLVQAPLVGLGSGIIPTLIAIFFTQLLWFF
GLHGQIIINSVMDPIWNTLSIENLNSYTATGEVPHIINKQFMEVYTVGMGGTGMTLA
>Mature_297_residues
MFEKLSNLLVPIAGKLNNNRYLGVLRDAFMLAFPLTIFGSIIVVLTNLPFLNKMMSKSALETFQSALSIAPSATISIMSV
FVVFGIGYYLSKTYEVEAVFGGVVALASFLLLTPFVLNGEGGEVIPNVIPIDRLGAKGMFLGMITAFTSAEIFRFFVQKN
YTIKMPAGVPPAVAKSFAALIPAVMTLSVYLVINILVTQAFNTNMHDLIYNLVQAPLVGLGSGIIPTLIAIFFTQLLWFF
GLHGQIIINSVMDPIWNTLSIENLNSYTATGEVPHIINKQFMEVYTVGMGGTGMTLA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1455

COG function: function code G; Phosphotransferase system cellobiose-specific component IIC

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-3 domain [H]

Homologues:

Organism=Escherichia coli, GI1788032, Length=315, Percent_Identity=30.4761904761905, Blast_Score=133, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003352
- InterPro:   IPR004796
- InterPro:   IPR004501 [H]

Pfam domain/function: PF02378 PTS_EIIC [H]

EC number: NA

Molecular weight: Translated: 32255; Mature: 32255

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: PS51105 PTS_EIIC_TYPE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEKLSNLLVPIAGKLNNNRYLGVLRDAFMLAFPLTIFGSIIVVLTNLPFLNKMMSKSAL
CHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
ETFQSALSIAPSATISIMSVFVVFGIGYYLSKTYEVEAVFGGVVALASFLLLTPFVLNGE
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHCCEEECCC
GGEVIPNVIPIDRLGAKGMFLGMITAFTSAEIFRFFVQKNYTIKMPAGVPPAVAKSFAAL
CCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHH
IPAVMTLSVYLVINILVTQAFNTNMHDLIYNLVQAPLVGLGSGIIPTLIAIFFTQLLWFF
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
GLHGQIIINSVMDPIWNTLSIENLNSYTATGEVPHIINKQFMEVYTVGMGGTGMTLA
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MFEKLSNLLVPIAGKLNNNRYLGVLRDAFMLAFPLTIFGSIIVVLTNLPFLNKMMSKSAL
CHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
ETFQSALSIAPSATISIMSVFVVFGIGYYLSKTYEVEAVFGGVVALASFLLLTPFVLNGE
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHCCEEECCC
GGEVIPNVIPIDRLGAKGMFLGMITAFTSAEIFRFFVQKNYTIKMPAGVPPAVAKSFAAL
CCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHH
IPAVMTLSVYLVINILVTQAFNTNMHDLIYNLVQAPLVGLGSGIIPTLIAIFFTQLLWFF
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
GLHGQIIINSVMDPIWNTLSIENLNSYTATGEVPHIINKQFMEVYTVGMGGTGMTLA
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: diacetylchitobiose [Periplasm]; phosphoenolpyruvate; cellobiose [Periplasm] [C]

Specific reaction: phosphoenolpyruvate + diacetylchitobiose [Periplasm] = pyruvate + diacetylchitobiose-6-phosphate [Cytoplasm] phosphoenolpyruvate + cellobiose [Periplasm] = cellobiose-6-phosphate [Cytoplasm] + pyruvate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9202461; 9384377 [H]