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The map label for this gene is ydhO [H]

Identifier: 52786244

GI number: 52786244

Start: 2433524

End: 2434870

Strand: Reverse

Name: ydhO [H]

Synonym: BLi02505

Alternate gene names: 52786244

Gene position: 2434870-2433524 (Counterclockwise)

Preceding gene: 52786245

Following gene: 52786243

Centisome position: 57.66

GC content: 47.44

Gene sequence:

>1347_bases
ATGAGCGGGTTTATGGAGAAGTTTGAGCGGAACGTGGAGCGGTTTTTGGTTCCCGTTGCTGCAAAATTGAATTCACAGCG
CCATATATGTGCGATCAGAGATGCTTTTATTCTATCATTTCCGATTATTATGGCAGGATCGATTATCATTTTATTGAATT
TTGCCATTTTATCTCCGGACGGGTTTATCGCCAAAATTTTATTTCTTGAAAAGATATTTCCGAACTTGGCTGATTACCAG
TCGGTCTTTACGCCCGTACTGCGGGGGTCGCTCGATATTATGTCCATATTTGTCGTGTTTCTCGTCGCCCGCAATTTAGC
CATCTCAATGAAAGCGGATGAACTGCTTGCCGGATTAACGGCTGTATCCTGTTATTTTATCATTTATTCACCGTACCGCC
TGATCGAAAACGAGAGCTTTTTAACGACGAAATGGCTCGGCGCCCAAGGACTGTTCGTCGCCTTAATCATCGGAATCATC
GTCGGTGAAGTGTTCAGCAGGCTATCAAATGCCAAGCGACTGCAAATTCATATGCCTCCGCAGGTGCCGCCTGCGGTCGC
CCGGACATTTAAAGTGCTGTTTCCGATCATCTTCATTACGATTGGTTTTGCGATATTAAGCTTTGCGCTGATGTCCCTGT
CGAAAAACGGTCTGCATGAATTGGTGTATACTGTGCTGCAGGCGCCGTTGAGAGAGATGGGAACGAATATTTTCACCGTT
GTTTTTCTTGGAATCGTCGCCAACTTTTTATGGCTGTTCGGCATTCACGGTCCGAATACGATTGCGGCGATCAGAGAAAC
GATTTTTGCAGAAGCAAACCTTGAAAACCTCGGTTTTGCCGCTAAAACAGGCTCTGCATGGAACGCTCCGTTTCCTGAAA
CATGGGCGCTGAATGACGCCTTTGCGAACTACGGGGGATCAGGCATGACGCTGGGGCTTTTGATCGCGATATTCATCGCT
TCAAAGCGGCGGGATTATAAAGATATCGGCAAGCTTTCGATCGGGCCGGGAATATTTAATATCAATGAACCTGTCATTTT
CGGACTTCCGGTCGTGCTCAATCCAATCTTCATGATTCCCTTTATTATCGTTCCGGCAGTCAATACTGTCATCGGCTATT
TGTTCATCAAGTTTCAGCTGATTCCGCCGATCGCATACGCTGTTCCGTGGACAACGCCAGGGCCGTTGATTCCGTTTCTC
GGGACCGGAGGAAACTGGCTTGCGCTCGGCGTCGGCTTTCTCTGCCTCGCCATTTCAACGATGATTTATCTGCCGTTTGT
GATGGCTGCCAACAAGACTGTGAATACGGACAGGGAGCATTCCGCGGAAAACAGGAAGGAATCATAA

Upstream 100 bases:

>100_bases
GTCCAGGTGATCGACATGCGAGACTACGGCACCATGAACGGCGAAGCTGTGCTGCAAAAAGCGCTGCAGCTGATGAAATA
AAAAAGGAGGGGAGATAGGG

Downstream 100 bases:

>100_bases
CTTTAGACGGGGCGCCTTTTACGGGCGCCCGTCTTTTTTTAAAAAAAGTCATGCGGCTCTCTTTTCTCTCATACAATCTA
TTAAAATCAAAAGCACGTCT

Product: YdhO

Products: pyruvate; diacetylchitobiose-6-phosphate [Cytoplasm]; cellobiose-6-phosphate [Cytoplasm] [C]

Alternate protein names: EIIC-Chb; PTS system N,N'-diacetylchitobiose-specific EIIC component [H]

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MSGFMEKFERNVERFLVPVAAKLNSQRHICAIRDAFILSFPIIMAGSIIILLNFAILSPDGFIAKILFLEKIFPNLADYQ
SVFTPVLRGSLDIMSIFVVFLVARNLAISMKADELLAGLTAVSCYFIIYSPYRLIENESFLTTKWLGAQGLFVALIIGII
VGEVFSRLSNAKRLQIHMPPQVPPAVARTFKVLFPIIFITIGFAILSFALMSLSKNGLHELVYTVLQAPLREMGTNIFTV
VFLGIVANFLWLFGIHGPNTIAAIRETIFAEANLENLGFAAKTGSAWNAPFPETWALNDAFANYGGSGMTLGLLIAIFIA
SKRRDYKDIGKLSIGPGIFNINEPVIFGLPVVLNPIFMIPFIIVPAVNTVIGYLFIKFQLIPPIAYAVPWTTPGPLIPFL
GTGGNWLALGVGFLCLAISTMIYLPFVMAANKTVNTDREHSAENRKES

Sequences:

>Translated_448_residues
MSGFMEKFERNVERFLVPVAAKLNSQRHICAIRDAFILSFPIIMAGSIIILLNFAILSPDGFIAKILFLEKIFPNLADYQ
SVFTPVLRGSLDIMSIFVVFLVARNLAISMKADELLAGLTAVSCYFIIYSPYRLIENESFLTTKWLGAQGLFVALIIGII
VGEVFSRLSNAKRLQIHMPPQVPPAVARTFKVLFPIIFITIGFAILSFALMSLSKNGLHELVYTVLQAPLREMGTNIFTV
VFLGIVANFLWLFGIHGPNTIAAIRETIFAEANLENLGFAAKTGSAWNAPFPETWALNDAFANYGGSGMTLGLLIAIFIA
SKRRDYKDIGKLSIGPGIFNINEPVIFGLPVVLNPIFMIPFIIVPAVNTVIGYLFIKFQLIPPIAYAVPWTTPGPLIPFL
GTGGNWLALGVGFLCLAISTMIYLPFVMAANKTVNTDREHSAENRKES
>Mature_447_residues
SGFMEKFERNVERFLVPVAAKLNSQRHICAIRDAFILSFPIIMAGSIIILLNFAILSPDGFIAKILFLEKIFPNLADYQS
VFTPVLRGSLDIMSIFVVFLVARNLAISMKADELLAGLTAVSCYFIIYSPYRLIENESFLTTKWLGAQGLFVALIIGIIV
GEVFSRLSNAKRLQIHMPPQVPPAVARTFKVLFPIIFITIGFAILSFALMSLSKNGLHELVYTVLQAPLREMGTNIFTVV
FLGIVANFLWLFGIHGPNTIAAIRETIFAEANLENLGFAAKTGSAWNAPFPETWALNDAFANYGGSGMTLGLLIAIFIAS
KRRDYKDIGKLSIGPGIFNINEPVIFGLPVVLNPIFMIPFIIVPAVNTVIGYLFIKFQLIPPIAYAVPWTTPGPLIPFLG
TGGNWLALGVGFLCLAISTMIYLPFVMAANKTVNTDREHSAENRKES

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-3 domain [H]

Homologues:

Organism=Escherichia coli, GI1788032, Length=453, Percent_Identity=34.878587196468, Blast_Score=239, Evalue=3e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003352
- InterPro:   IPR004796
- InterPro:   IPR004501 [H]

Pfam domain/function: PF02378 PTS_EIIC [H]

EC number: NA

Molecular weight: Translated: 49292; Mature: 49161

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: PS51105 PTS_EIIC_TYPE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGFMEKFERNVERFLVPVAAKLNSQRHICAIRDAFILSFPIIMAGSIIILLNFAILSPD
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GFIAKILFLEKIFPNLADYQSVFTPVLRGSLDIMSIFVVFLVARNLAISMKADELLAGLT
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEECHHHHHHHHH
AVSCYFIIYSPYRLIENESFLTTKWLGAQGLFVALIIGIIVGEVFSRLSNAKRLQIHMPP
HHHHHHHHCCHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
QVPPAVARTFKVLFPIIFITIGFAILSFALMSLSKNGLHELVYTVLQAPLREMGTNIFTV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHH
VFLGIVANFLWLFGIHGPNTIAAIRETIFAEANLENLGFAAKTGSAWNAPFPETWALNDA
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHCCCEECCCCCCCCCCCCCCCCCHH
FANYGGSGMTLGLLIAIFIASKRRDYKDIGKLSIGPGIFNINEPVIFGLPVVLNPIFMIP
HHHCCCCCHHHHHHHHHHHHHCCCCHHHHCCEECCCCEEECCCCCEECCHHHHHHHHHHH
FIIVPAVNTVIGYLFIKFQLIPPIAYAVPWTTPGPLIPFLGTGGNWLALGVGFLCLAIST
HHHHHHHHHHHHHHHHHHHHCCCHHEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
MIYLPFVMAANKTVNTDREHSAENRKES
HHHHHHHHHCCCCCCCCHHHCCCCCCCC
>Mature Secondary Structure 
SGFMEKFERNVERFLVPVAAKLNSQRHICAIRDAFILSFPIIMAGSIIILLNFAILSPD
CHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GFIAKILFLEKIFPNLADYQSVFTPVLRGSLDIMSIFVVFLVARNLAISMKADELLAGLT
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEECHHHHHHHHH
AVSCYFIIYSPYRLIENESFLTTKWLGAQGLFVALIIGIIVGEVFSRLSNAKRLQIHMPP
HHHHHHHHCCHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
QVPPAVARTFKVLFPIIFITIGFAILSFALMSLSKNGLHELVYTVLQAPLREMGTNIFTV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHH
VFLGIVANFLWLFGIHGPNTIAAIRETIFAEANLENLGFAAKTGSAWNAPFPETWALNDA
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHCCCEECCCCCCCCCCCCCCCCCHH
FANYGGSGMTLGLLIAIFIASKRRDYKDIGKLSIGPGIFNINEPVIFGLPVVLNPIFMIP
HHHCCCCCHHHHHHHHHHHHHCCCCHHHHCCEECCCCEEECCCCCEECCHHHHHHHHHHH
FIIVPAVNTVIGYLFIKFQLIPPIAYAVPWTTPGPLIPFLGTGGNWLALGVGFLCLAIST
HHHHHHHHHHHHHHHHHHHHCCCHHEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
MIYLPFVMAANKTVNTDREHSAENRKES
HHHHHHHHHCCCCCCCCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: diacetylchitobiose [Periplasm]; phosphoenolpyruvate; cellobiose [Periplasm] [C]

Specific reaction: phosphoenolpyruvate + diacetylchitobiose [Periplasm] = pyruvate + diacetylchitobiose-6-phosphate [Cytoplasm] phosphoenolpyruvate + cellobiose [Periplasm] = cellobiose-6-phosphate [Cytoplasm] + pyruvate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2179047; 9097039; 9278503; 2092358; 9405618; 10913117 [H]