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The map label for this gene is sigF [H]

Identifier: 52786234

GI number: 52786234

Start: 2425745

End: 2426512

Strand: Reverse

Name: sigF [H]

Synonym: BLi02495

Alternate gene names: 52786234

Gene position: 2426512-2425745 (Counterclockwise)

Preceding gene: 52786235

Following gene: 52786233

Centisome position: 57.46

GC content: 47.01

Gene sequence:

>768_bases
ATGGATGTGGAGGTTAAAAAAGAAAACCAGAACACTCAGCTTAAAGACCATGAAGTGAAAGAACTGATTAAAAACAGCCA
GAACGGCGATCAAAAAGCAAGGGACCTCCTCATAGAAAAAAACATGCGTCTTGTTTGGTCTGTCGTTCAGCGTTTTTTGA
ACAGAGGCTATGAGCCTGACGACCTCTTTCAAATCGGCTGCATCGGCCTCTTGAAGTCGGTGGACAAATTCGATCTTTCC
TATGACGTTCGGTTTTCCACCTACGCCGTTCCGATGATTATCGGCGAGATTCAGCGGTTTATCAGAGATGACGGAACCGT
CAAAGTGAGCCGCTCGCTGAAAGAACTCGGCAACAAAATCCGGCGGGCGAAAGACGAGCTTTCCAAGTCAAACGGCCGGA
TTCCGACCGTTCAGGAAATCGCCGATTATCTCGAAATCAGTTCAGAAGAGGTCGTGATGGCCCAGGAAGCGGTCCGCTCT
CCCTCGTCCATTCATGAAACGGTTTACGAAAACGACGGGGACCCGATTACACTTCTGGACCAAATCGCCGATCAGTCGGA
AGAGAAGTGGTTCGATAAAATCGCTTTGAAGGAAGCCATCAAGGATTTGGATGAACGGGAAAAGCTGATTGTTTATTTAA
GGTATTACAAAGACAAGACGCAATCTGAAGTTGCCGACCGCCTCGGCATATCCCAGGTGCAAGTCTCGAGGCTTGAGAAA
AAGATTTTAAAGCAAATCAAAAACCAGATGGACCATTTTGAAAGCTGA

Upstream 100 bases:

>100_bases
TGATATTTCGATCGACTCCTCACCTGAGATGGGAACCACAATACACTTAACAAAGCACTTATCAAAAAGCAAAGCGCTTT
GCAATTAAGGGAGATTTGTT

Downstream 100 bases:

>100_bases
GGCTGCTCATGTTGCAGGCAGCCTCGGATGTCCGATGAAAAACCGGACGCTCTTGGGAGCGTTCCGGTTTTTTTGTGTGG
TAATTTATGGTCTTTTGCGC

Product: sporulation sigma factor SigF

Products: NA

Alternate protein names: Sporulation sigma factor; Stage II sporulation protein AC [H]

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MDVEVKKENQNTQLKDHEVKELIKNSQNGDQKARDLLIEKNMRLVWSVVQRFLNRGYEPDDLFQIGCIGLLKSVDKFDLS
YDVRFSTYAVPMIIGEIQRFIRDDGTVKVSRSLKELGNKIRRAKDELSKSNGRIPTVQEIADYLEISSEEVVMAQEAVRS
PSSIHETVYENDGDPITLLDQIADQSEEKWFDKIALKEAIKDLDEREKLIVYLRYYKDKTQSEVADRLGISQVQVSRLEK
KILKQIKNQMDHFES

Sequences:

>Translated_255_residues
MDVEVKKENQNTQLKDHEVKELIKNSQNGDQKARDLLIEKNMRLVWSVVQRFLNRGYEPDDLFQIGCIGLLKSVDKFDLS
YDVRFSTYAVPMIIGEIQRFIRDDGTVKVSRSLKELGNKIRRAKDELSKSNGRIPTVQEIADYLEISSEEVVMAQEAVRS
PSSIHETVYENDGDPITLLDQIADQSEEKWFDKIALKEAIKDLDEREKLIVYLRYYKDKTQSEVADRLGISQVQVSRLEK
KILKQIKNQMDHFES
>Mature_255_residues
MDVEVKKENQNTQLKDHEVKELIKNSQNGDQKARDLLIEKNMRLVWSVVQRFLNRGYEPDDLFQIGCIGLLKSVDKFDLS
YDVRFSTYAVPMIIGEIQRFIRDDGTVKVSRSLKELGNKIRRAKDELSKSNGRIPTVQEIADYLEISSEEVVMAQEAVRS
PSSIHETVYENDGDPITLLDQIADQSEEKWFDKIALKEAIKDLDEREKLIVYLRYYKDKTQSEVADRLGISQVQVSRLEK
KILKQIKNQMDHFES

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=254, Percent_Identity=29.1338582677165, Blast_Score=94, Evalue=6e-21,
Organism=Escherichia coli, GI1789098, Length=218, Percent_Identity=29.8165137614679, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789871, Length=266, Percent_Identity=28.1954887218045, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788231, Length=199, Percent_Identity=29.1457286432161, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014322
- InterPro:   IPR014236
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 29628; Mature: 29628

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHCCC
>Mature Secondary Structure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HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2513372 [H]