Click here to switch to the map view.

The map label for this gene is dacB [H]

Identifier: 52786204

GI number: 52786204

Start: 2400887

End: 2402041

Strand: Reverse

Name: dacB [H]

Synonym: BLi02465

Alternate gene names: 52786204

Gene position: 2402041-2400887 (Counterclockwise)

Preceding gene: 52786205

Following gene: 52786203

Centisome position: 56.88

GC content: 44.42

Gene sequence:

>1155_bases
ATGCGCTTTTTTCTAAAACAAGCGGCAGCAGCCATCATGATATGCTCTCTTTTATGCTCTTCATACGAAACCGCTCAAGC
ACAGCCCTCCCTTCATATCAGCGCAAAAAGCGCGATTGTCATGGACGGGCAATCCGGACGGGTTTTGTTTGCAAAGGATG
AGCATGAAAAACGGCGCATTGCAAGCATTACGAAGATTATGACAGCCATTTTGGCCGTGGAATCGGGCAAATTGGATGAG
ACGGTCACGGTCAGCGACAGAGCGGTCAGGACAGAGGGATCATCGATCTATTTAACAAGCGGGCAAAAGGTGAAGCTGAA
GGACCTTGTTTACGGTTTGATGCTGAGATCGGGAAATGATGCAGCCGTTGCGATTGCCGAACATGTGGGCGGAAGCCTGG
AAGGCTTTGTCTACATGATGAATCAAAAAGCGGCCGAGCTCGGCATGGAAAACACCCTGTTCCGCAATCCGCACGGACTT
GATGACCACCCTGATCACTATTCCTCGGCCTATGATATGGCGCTTTTAACAAAATATGCGATGACAAATGAAACGTACAA
AAAAATCGCCGGAACGAAACGCTATAAAGCAGAAACGATGCAAGGCATCTGGAGAAATAAAAACAAGCTTTTAACAGGGC
TTTACAAATACAGTACAGGCGGGAAGACGGGATATACAAAGCTTGCAAAGCGTACGCTCGTCTCCACTTCATCGAAAGAC
GGAACCGATTTGATCGCCGTCACAATCAATGCCCCTGACGACTGGAATGATCATATGAACATGTTCAACTATGTATTCGG
CCACTACAAAACATATATCATCGCCAAAAAAGGCGAGATTCCGAAATTAAAAGACTCTTTTTACGGAAATAAAGCTTTTA
TTAAACGGGATGTCACATATCTTTTAAACGAAGAGGAAAAAGAAGATGTGAAGGTTGATATCGAGCTTCTTGAACCGAAA
AAATCATGGCGTAAAAACAAAAAAGAAATCCCGGACATCATCGGAGAAATGAACGTCATGTTCGACGGAAAAACGATTGC
AAGCGTACCGATCTATTATGAAAACGAGCGAAACAAAAAACCGAAAAAATCGTTTTTCGAGACCTTTCAATCCGTATTCC
AAAAAGCGGCGGGCGGTTCATCATGGTCAATATAA

Upstream 100 bases:

>100_bases
TCTTGCTTTAGATTTTTCTCGGGAAATCATCCAATTGGTGTTCATAACAAAAAGACAAGCGCATAAACTTGTACAAACAA
CCACAAGGACGTGAGAAAAA

Downstream 100 bases:

>100_bases
TCTGGGTCGGCTTAACGGTGATCGGTCTGGTGTTTGCGCTTTTCAACGGAACGGTTCAGGAAGTTAATGAAGCTGTATTT
AAAGGATCGAAGGAAGCCGT

Product: DacB

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5* [H]

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MRFFLKQAAAAIMICSLLCSSYETAQAQPSLHISAKSAIVMDGQSGRVLFAKDEHEKRRIASITKIMTAILAVESGKLDE
TVTVSDRAVRTEGSSIYLTSGQKVKLKDLVYGLMLRSGNDAAVAIAEHVGGSLEGFVYMMNQKAAELGMENTLFRNPHGL
DDHPDHYSSAYDMALLTKYAMTNETYKKIAGTKRYKAETMQGIWRNKNKLLTGLYKYSTGGKTGYTKLAKRTLVSTSSKD
GTDLIAVTINAPDDWNDHMNMFNYVFGHYKTYIIAKKGEIPKLKDSFYGNKAFIKRDVTYLLNEEEKEDVKVDIELLEPK
KSWRKNKKEIPDIIGEMNVMFDGKTIASVPIYYENERNKKPKKSFFETFQSVFQKAAGGSSWSI

Sequences:

>Translated_384_residues
MRFFLKQAAAAIMICSLLCSSYETAQAQPSLHISAKSAIVMDGQSGRVLFAKDEHEKRRIASITKIMTAILAVESGKLDE
TVTVSDRAVRTEGSSIYLTSGQKVKLKDLVYGLMLRSGNDAAVAIAEHVGGSLEGFVYMMNQKAAELGMENTLFRNPHGL
DDHPDHYSSAYDMALLTKYAMTNETYKKIAGTKRYKAETMQGIWRNKNKLLTGLYKYSTGGKTGYTKLAKRTLVSTSSKD
GTDLIAVTINAPDDWNDHMNMFNYVFGHYKTYIIAKKGEIPKLKDSFYGNKAFIKRDVTYLLNEEEKEDVKVDIELLEPK
KSWRKNKKEIPDIIGEMNVMFDGKTIASVPIYYENERNKKPKKSFFETFQSVFQKAAGGSSWSI
>Mature_384_residues
MRFFLKQAAAAIMICSLLCSSYETAQAQPSLHISAKSAIVMDGQSGRVLFAKDEHEKRRIASITKIMTAILAVESGKLDE
TVTVSDRAVRTEGSSIYLTSGQKVKLKDLVYGLMLRSGNDAAVAIAEHVGGSLEGFVYMMNQKAAELGMENTLFRNPHGL
DDHPDHYSSAYDMALLTKYAMTNETYKKIAGTKRYKAETMQGIWRNKNKLLTGLYKYSTGGKTGYTKLAKRTLVSTSSKD
GTDLIAVTINAPDDWNDHMNMFNYVFGHYKTYIIAKKGEIPKLKDSFYGNKAFIKRDVTYLLNEEEKEDVKVDIELLEPK
KSWRKNKKEIPDIIGEMNVMFDGKTIASVPIYYENERNKKPKKSFFETFQSVFQKAAGGSSWSI

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex) [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=368, Percent_Identity=30.1630434782609, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI87082022, Length=235, Percent_Identity=36.1702127659575, Blast_Score=121, Evalue=8e-29,
Organism=Escherichia coli, GI1786851, Length=386, Percent_Identity=29.5336787564767, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI87082054, Length=235, Percent_Identity=30.2127659574468, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 43152; Mature: 43152

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFFLKQAAAAIMICSLLCSSYETAQAQPSLHISAKSAIVMDGQSGRVLFAKDEHEKRRI
CCHHHHHHHHHHHHHHHHHCCCHHCCCCCCEEEECCEEEEEECCCCCEEEECCCHHHHHH
ASITKIMTAILAVESGKLDETVTVSDRAVRTEGSSIYLTSGQKVKLKDLVYGLMLRSGND
HHHHHHHHHHHHHCCCCCCCEEEECCCEEEECCCEEEEECCCEEEHHHHHHHHHHCCCCC
AAVAIAEHVGGSLEGFVYMMNQKAAELGMENTLFRNPHGLDDHPDHYSSAYDMALLTKYA
CEEEEHHHCCCCCCCEEEEECHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
MTNETYKKIAGTKRYKAETMQGIWRNKNKLLTGLYKYSTGGKTGYTKLAKRTLVSTSSKD
HHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHEEECCCCCCHHHHHHHHHHHCCCCCC
GTDLIAVTINAPDDWNDHMNMFNYVFGHYKTYIIAKKGEIPKLKDSFYGNKAFIKRDVTY
CCEEEEEEEECCCCCCHHHHHHHHHHHCEEEEEEEECCCCCCHHHHCCCCHHHHHHHHHH
LLNEEEKEDVKVDIELLEPKKSWRKNKKEIPDIIGEMNVMFDGKTIASVPIYYENERNKK
HCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHCCCEEEECCCEEEEEEEEECCCCCCC
PKKSFFETFQSVFQKAAGGSSWSI
HHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRFFLKQAAAAIMICSLLCSSYETAQAQPSLHISAKSAIVMDGQSGRVLFAKDEHEKRRI
CCHHHHHHHHHHHHHHHHHCCCHHCCCCCCEEEECCEEEEEECCCCCEEEECCCHHHHHH
ASITKIMTAILAVESGKLDETVTVSDRAVRTEGSSIYLTSGQKVKLKDLVYGLMLRSGND
HHHHHHHHHHHHHCCCCCCCEEEECCCEEEECCCEEEEECCCEEEHHHHHHHHHHCCCCC
AAVAIAEHVGGSLEGFVYMMNQKAAELGMENTLFRNPHGLDDHPDHYSSAYDMALLTKYA
CEEEEHHHCCCCCCCEEEEECHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
MTNETYKKIAGTKRYKAETMQGIWRNKNKLLTGLYKYSTGGKTGYTKLAKRTLVSTSSKD
HHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHEEECCCCCCHHHHHHHHHHHCCCCCC
GTDLIAVTINAPDDWNDHMNMFNYVFGHYKTYIIAKKGEIPKLKDSFYGNKAFIKRDVTY
CCEEEEEEEECCCCCCHHHHHHHHHHHCEEEEEEEECCCCCCHHHHCCCCHHHHHHHHHH
LLNEEEKEDVKVDIELLEPKKSWRKNKKEIPDIIGEMNVMFDGKTIASVPIYYENERNKK
HCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHCCCEEEECCCEEEEEEEEECCCCCCC
PKKSFFETFQSVFQKAAGGSSWSI
HHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377 [H]