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The map label for this gene is ansA [H]

Identifier: 52786175

GI number: 52786175

Start: 2371047

End: 2372015

Strand: Reverse

Name: ansA [H]

Synonym: BLi02433

Alternate gene names: 52786175

Gene position: 2372015-2371047 (Counterclockwise)

Preceding gene: 52786176

Following gene: 52786174

Centisome position: 56.17

GC content: 48.81

Gene sequence:

>969_bases
ATGAATAAAAAAGTAGCTCTCATTACAACGGGAGGAACGATCGCCAGCCGGAAAACCGAAAGCGGCCGGCTTGCGGCAGG
GGCGATCAGCGGCCCTGAATTAGCCGAAATGTGCAGCCTGCCGGAAGATGTACAGATCGATGTCTACCCGGCGTTTCAGC
TTCCAAGTATGCATATAACATTTCAGCATTTATTAGAGCTCAAGCAAACCATCGAACGCGTGTTTCAGGACGGCGGCTAT
GACGGCGCGGTTGTAACGCATGGTACGGACACGCTTGAAGAAACCGCCTATTTTTTGGATTTAACAATAGAGGATGAGAG
GCCGGTCGTTGTCACAGGTTCACAGCGAGCACCGGAACAGCAAGGCACAGATGCATATACAAATATCAGGCATGCCGTAT
ATACAGCTTGCAGTCCGGATATAAAAGGAGCCGGCACGGTTGTTGTTTTTAACGAAAGGATCTTTAACGCGCGCTACGTT
AAAAAAGTGCACGCTTCGAATCTGCAGGGTTTTGACGTATTCGGTTTCGGCTACTTAGGGATCATTGATAACGACAAAGT
ATACGTTTATCAAAAGCCGTTAAAGCGGGATGTTCACCAGCTGCAAAGGCCGCTTCCTGCAGTGGACATCGTCAAATGCT
ATCTTGATGGAGACGGCAAGTTTATCAGAGCCGCCGTCCGCGAGGGTGTCGAAGGGATCGTCCTTGAGGGAGTCGGAAGG
GGGCAGGTGCCGCCGAATATGATGGCTGATATCGAACAAGCTTTAAATCAGGGCGTTTATATCGTCATCACGACGAGCGC
AGAGGAAGGCGAAGTTTATACGACCTATGACTATGCAGGCAGCAGCTATGACCTCGCGAAAAAAGGGGTCATTTTAGGCA
AGGATTATGACAGCAAAAAAGCGAGAATGAAACTCGCCGTCCTTCTTGCAAGCTATAAAGAAGGCATAAAAGACAAATTC
TGCTATTAG

Upstream 100 bases:

>100_bases
GTTTTATGATTTGTATCAGGCAAAAGAGAGCATATTCTATATATAGATGTGCTCTCTTTTTTATCGGCACACAGATCAAC
GCGAAAAGGGGCATCTTGAC

Downstream 100 bases:

>100_bases
GCGGCCTGTTCTTTTTCAATTTCAAAACAATCCGAATCATGGTAGGATAGAATGAGATGAAAAGGAAAGAGGTTTCCTCA
TGTTCGGAATTATCTCAGCA

Product: hypothetical protein

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MNKKVALITTGGTIASRKTESGRLAAGAISGPELAEMCSLPEDVQIDVYPAFQLPSMHITFQHLLELKQTIERVFQDGGY
DGAVVTHGTDTLEETAYFLDLTIEDERPVVVTGSQRAPEQQGTDAYTNIRHAVYTACSPDIKGAGTVVVFNERIFNARYV
KKVHASNLQGFDVFGFGYLGIIDNDKVYVYQKPLKRDVHQLQRPLPAVDIVKCYLDGDGKFIRAAVREGVEGIVLEGVGR
GQVPPNMMADIEQALNQGVYIVITTSAEEGEVYTTYDYAGSSYDLAKKGVILGKDYDSKKARMKLAVLLASYKEGIKDKF
CY

Sequences:

>Translated_322_residues
MNKKVALITTGGTIASRKTESGRLAAGAISGPELAEMCSLPEDVQIDVYPAFQLPSMHITFQHLLELKQTIERVFQDGGY
DGAVVTHGTDTLEETAYFLDLTIEDERPVVVTGSQRAPEQQGTDAYTNIRHAVYTACSPDIKGAGTVVVFNERIFNARYV
KKVHASNLQGFDVFGFGYLGIIDNDKVYVYQKPLKRDVHQLQRPLPAVDIVKCYLDGDGKFIRAAVREGVEGIVLEGVGR
GQVPPNMMADIEQALNQGVYIVITTSAEEGEVYTTYDYAGSSYDLAKKGVILGKDYDSKKARMKLAVLLASYKEGIKDKF
CY
>Mature_322_residues
MNKKVALITTGGTIASRKTESGRLAAGAISGPELAEMCSLPEDVQIDVYPAFQLPSMHITFQHLLELKQTIERVFQDGGY
DGAVVTHGTDTLEETAYFLDLTIEDERPVVVTGSQRAPEQQGTDAYTNIRHAVYTACSPDIKGAGTVVVFNERIFNARYV
KKVHASNLQGFDVFGFGYLGIIDNDKVYVYQKPLKRDVHQLQRPLPAVDIVKCYLDGDGKFIRAAVREGVEGIVLEGVGR
GQVPPNMMADIEQALNQGVYIVITTSAEEGEVYTTYDYAGSSYDLAKKGVILGKDYDSKKARMKLAVLLASYKEGIKDKF
CY

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=284, Percent_Identity=25, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI1789327, Length=241, Percent_Identity=33.6099585062241, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1788065, Length=326, Percent_Identity=27.3006134969325, Blast_Score=92, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17558280, Length=274, Percent_Identity=28.8321167883212, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17558278, Length=274, Percent_Identity=28.8321167883212, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI193207374, Length=274, Percent_Identity=28.8321167883212, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI193207372, Length=274, Percent_Identity=28.8321167883212, Blast_Score=88, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6323189, Length=251, Percent_Identity=35.4581673306773, Blast_Score=137, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6323187, Length=251, Percent_Identity=35.4581673306773, Blast_Score=137, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6323186, Length=251, Percent_Identity=35.4581673306773, Blast_Score=137, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6323184, Length=251, Percent_Identity=35.4581673306773, Blast_Score=137, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6320527, Length=262, Percent_Identity=29.3893129770992, Blast_Score=101, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24645530, Length=350, Percent_Identity=26.2857142857143, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24639609, Length=276, Percent_Identity=26.4492753623188, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 35427; Mature: 35427

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKVALITTGGTIASRKTESGRLAAGAISGPELAEMCSLPEDVQIDVYPAFQLPSMHIT
CCCEEEEEEECCEEECCCCCCCCEEECCCCCHHHHHHHCCCCCCEEEEEECCCCCCEEEH
FQHLLELKQTIERVFQDGGYDGAVVTHGTDTLEETAYFLDLTIEDERPVVVTGSQRAPEQ
HHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHCCEEEEEEEECCCCCEEEECCCCCCCC
QGTDAYTNIRHAVYTACSPDIKGAGTVVVFNERIFNARYVKKVHASNLQGFDVFGFGYLG
CCCCHHHHHHHHEEECCCCCCCCCCEEEEECCCHHCHHHHHHHHHCCCCCEEEECCEEEE
IIDNDKVYVYQKPLKRDVHQLQRPLPAVDIVKCYLDGDGKFIRAAVREGVEGIVLEGVGR
EEECCEEEEECCHHHHHHHHHHCCCCCEEEEEEEECCCCCEEHHHHHCCCCEEEEECCCC
GQVPPNMMADIEQALNQGVYIVITTSAEEGEVYTTYDYAGSSYDLAKKGVILGKDYDSKK
CCCCHHHHHHHHHHHCCCEEEEEEECCCCCCEEEEEECCCCCCCHHHCCEEEECCCCCHH
ARMKLAVLLASYKEGIKDKFCY
HHHHHHHHHHHHHHCCHHCCCC
>Mature Secondary Structure
MNKKVALITTGGTIASRKTESGRLAAGAISGPELAEMCSLPEDVQIDVYPAFQLPSMHIT
CCCEEEEEEECCEEECCCCCCCCEEECCCCCHHHHHHHCCCCCCEEEEEECCCCCCEEEH
FQHLLELKQTIERVFQDGGYDGAVVTHGTDTLEETAYFLDLTIEDERPVVVTGSQRAPEQ
HHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHCCEEEEEEEECCCCCEEEECCCCCCCC
QGTDAYTNIRHAVYTACSPDIKGAGTVVVFNERIFNARYVKKVHASNLQGFDVFGFGYLG
CCCCHHHHHHHHEEECCCCCCCCCCEEEEECCCHHCHHHHHHHHHCCCCCEEEECCEEEE
IIDNDKVYVYQKPLKRDVHQLQRPLPAVDIVKCYLDGDGKFIRAAVREGVEGIVLEGVGR
EEECCEEEEECCHHHHHHHHHHCCCCCEEEEEEEECCCCCEEHHHHHCCCCEEEEECCCC
GQVPPNMMADIEQALNQGVYIVITTSAEEGEVYTTYDYAGSSYDLAKKGVILGKDYDSKK
CCCCHHHHHHHHHHHCCCEEEEEEECCCCCCEEEEEECCCCCCCHHHCCEEEECCCCCHH
ARMKLAVLLASYKEGIKDKFCY
HHHHHHHHHHHHHHCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1916233 [H]