Click here to switch to the map view.

The map label for this gene is sleB [H]

Identifier: 52786173

GI number: 52786173

Start: 2369195

End: 2370160

Strand: Reverse

Name: sleB [H]

Synonym: BLi02431

Alternate gene names: 52786173

Gene position: 2370160-2369195 (Counterclockwise)

Preceding gene: 52786174

Following gene: 52786172

Centisome position: 56.13

GC content: 49.07

Gene sequence:

>966_bases
ATGAAGTCGAAAGGTTCTTTTTGTTTGAATGTCATTTTATGTACCATTATGGCAACCAGTCTTTCACTTCCATTCAGTCA
AAAAAGCGCTGCCTTTTCTGAACAGGTAATACAGCGGGGGGCGACAGGGGATGATGTGATTGAACTGCAGGCAAGACTTC
AATACAATGGCTATTACAATGGCAGAATTGACGGAGTATACGGGTGGGCGACATACTGGGCCGTCCGCAATTTCCAAAAT
CAGTTCGGCTTAAGGGATACAGACGGCCTGGTCGGGCCGAAAACAAAACGATTATTAATCAATCATACTAAATATTACAG
CGACTACGTGCACAGACAGCTTCGAAAAGGAAACCAATTTACACATTACGGCGGGCTGCCGCTGAAGTATCAGACGAAGC
CTTCAAAAGAAGTGCAGCAGCAGGCGAGAAAAAAGGCGGAAGCAAGACAGCGGCAGGGCGCACAACAGCAGGGTACCGCC
CAAAAACAGCCGCAGCAAAAGCAGCAGGCCGCTCCTGCACAGCCGAAAAAACAAGCACAGCCAAAGGCTGCACCTAAACA
AGCCGCGCCTAAAAAACAGGATGCCGTTGCAGCCAATATGCCGGGCGGATTCTCGAACAACGACATTAATCTGTTGGCCC
AAGCGGTTTACGGTGAAGCGCGCGGAGAACCGTATGACGGCCAGGTTGCCGTTGCAGCTGTTATATTAAACAGGCTCGAT
AGCCCTACGTTCCCGAATACGGTGGCGGGCGTTATCTTTGAACCGCTTGCATTTACAGCTGTCGCGGACGGACAGATTTA
TATGACGCCGGATGAAACGGCGAAAAAAGCCGTTCTTGACGCCATCAACGGCTGGGATCCATCAGAAAATGCCACTTACT
ATTTTAACCCTGACACGGCCACTAGCTCATGGATTTGGGGCAGACCTCAAATTAAAAGGATCGGTAAACACATTTTCTGT
GAATAA

Upstream 100 bases:

>100_bases
AGGCATATTTGCCTAAAGATGAAACAGAAAGGAAAGCGTGTATAAAATCTGCCTCGCTACAAAAGATATGGTTACAAAAA
TGCTGGAGGAGGCTTAAAAC

Downstream 100 bases:

>100_bases
AAGCGAGGTGCTATAAATGATCAGAGGAATTTTAATCGCCCTTTTAGGTGTCGCCATCGTCGGAACAAGCTATTGGGGCT
ATAAAGAACATCAGGAGAAA

Product: hypothetical protein

Products: NA

Alternate protein names: SCLE [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MKSKGSFCLNVILCTIMATSLSLPFSQKSAAFSEQVIQRGATGDDVIELQARLQYNGYYNGRIDGVYGWATYWAVRNFQN
QFGLRDTDGLVGPKTKRLLINHTKYYSDYVHRQLRKGNQFTHYGGLPLKYQTKPSKEVQQQARKKAEARQRQGAQQQGTA
QKQPQQKQQAAPAQPKKQAQPKAAPKQAAPKKQDAVAANMPGGFSNNDINLLAQAVYGEARGEPYDGQVAVAAVILNRLD
SPTFPNTVAGVIFEPLAFTAVADGQIYMTPDETAKKAVLDAINGWDPSENATYYFNPDTATSSWIWGRPQIKRIGKHIFC
E

Sequences:

>Translated_321_residues
MKSKGSFCLNVILCTIMATSLSLPFSQKSAAFSEQVIQRGATGDDVIELQARLQYNGYYNGRIDGVYGWATYWAVRNFQN
QFGLRDTDGLVGPKTKRLLINHTKYYSDYVHRQLRKGNQFTHYGGLPLKYQTKPSKEVQQQARKKAEARQRQGAQQQGTA
QKQPQQKQQAAPAQPKKQAQPKAAPKQAAPKKQDAVAANMPGGFSNNDINLLAQAVYGEARGEPYDGQVAVAAVILNRLD
SPTFPNTVAGVIFEPLAFTAVADGQIYMTPDETAKKAVLDAINGWDPSENATYYFNPDTATSSWIWGRPQIKRIGKHIFC
E
>Mature_321_residues
MKSKGSFCLNVILCTIMATSLSLPFSQKSAAFSEQVIQRGATGDDVIELQARLQYNGYYNGRIDGVYGWATYWAVRNFQN
QFGLRDTDGLVGPKTKRLLINHTKYYSDYVHRQLRKGNQFTHYGGLPLKYQTKPSKEVQQQARKKAEARQRQGAQQQGTA
QKQPQQKQQAAPAQPKKQAQPKAAPKQAAPKKQDAVAANMPGGFSNNDINLLAQAVYGEARGEPYDGQVAVAAVILNRLD
SPTFPNTVAGVIFEPLAFTAVADGQIYMTPDETAKKAVLDAINGWDPSENATYYFNPDTATSSWIWGRPQIKRIGKHIFC
E

Specific function: Could be a lytic transglycosylase. Required for spore cortex hydrolysis during germination. Interacts strongly but noncovalently with spore components [H]

COG id: COG3773

COG function: function code M; Cell wall hydrolyses involved in spore germination

Gene ontology:

Cell location: Forespore. Note=Expressed in the forespore and then transported across the inner forespore membrane and deposited on the outside of the cortex [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sleB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011105
- InterPro:   IPR002477
- InterPro:   IPR014224 [H]

Pfam domain/function: PF07486 Hydrolase_2; PF01471 PG_binding_1 [H]

EC number: 3.5.1.28

Molecular weight: Translated: 35581; Mature: 35581

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSKGSFCLNVILCTIMATSLSLPFSQKSAAFSEQVIQRGATGDDVIELQARLQYNGYYN
CCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHEEEHHEECCEEC
GRIDGVYGWATYWAVRNFQNQFGLRDTDGLVGPKTKRLLINHTKYYSDYVHRQLRKGNQF
CEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHEEEHHHHHHHHHHHHHHHCCCCC
THYGGLPLKYQTKPSKEVQQQARKKAEARQRQGAQQQGTAQKQPQQKQQAAPAQPKKQAQ
EEECCCCEEECCCCHHHHHHHHHHHHHHHHHCCCHHCCCCCCCHHHHHHCCCCCCHHHCC
PKAAPKQAAPKKQDAVAANMPGGFSNNDINLLAQAVYGEARGEPYDGQVAVAAVILNRLD
CCCCCHHCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCC
SPTFPNTVAGVIFEPLAFTAVADGQIYMTPDETAKKAVLDAINGWDPSENATYYFNPDTA
CCCCCHHHHHHHHHHHHHEEEECCEEEECCCHHHHHHHHHHHCCCCCCCCCEEEECCCCC
TSSWIWGRPQIKRIGKHIFCE
CCCEECCCHHHHHHHHHHCCC
>Mature Secondary Structure
MKSKGSFCLNVILCTIMATSLSLPFSQKSAAFSEQVIQRGATGDDVIELQARLQYNGYYN
CCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHEEEHHEECCEEC
GRIDGVYGWATYWAVRNFQNQFGLRDTDGLVGPKTKRLLINHTKYYSDYVHRQLRKGNQF
CEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHEEEHHHHHHHHHHHHHHHCCCCC
THYGGLPLKYQTKPSKEVQQQARKKAEARQRQGAQQQGTAQKQPQQKQQAAPAQPKKQAQ
EEECCCCEEECCCCHHHHHHHHHHHHHHHHHCCCHHCCCCCCCHHHHHHCCCCCCHHHCC
PKAAPKQAAPKKQDAVAANMPGGFSNNDINLLAQAVYGEARGEPYDGQVAVAAVILNRLD
CCCCCHHCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCC
SPTFPNTVAGVIFEPLAFTAVADGQIYMTPDETAKKAVLDAINGWDPSENATYYFNPDTA
CCCCCHHHHHHHHHHHHHEEEECCEEEECCCHHHHHHHHHHHCCCCCCCCCEEEECCCCC
TSSWIWGRPQIKRIGKHIFCE
CCCEECCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8830707; 8760912; 9384377; 10197998; 10658652 [H]