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The map label for this gene is ponA [H]

Identifier: 52786111

GI number: 52786111

Start: 2312137

End: 2314824

Strand: Direct

Name: ponA [H]

Synonym: BLi02367

Alternate gene names: 52786111

Gene position: 2312137-2314824 (Clockwise)

Preceding gene: 52786110

Following gene: 52786130

Centisome position: 54.76

GC content: 47.66

Gene sequence:

>2688_bases
ATGTCAGATCAATTTAACAGCCGCCAGGAACGGCGAAAAGCCCAGCAGGGCAAAAGCAGGTCTAATACTCATTCTAAACC
CAAGAAAAAGAAAAAAGCAGGATTGTTTAAGAAAATTCTTTTATCCATTTTAATCATCGGTGTTATCGGCTTGATTGCCG
GGGGCGTCACTTTTGCGGTGATGGTAGCGGACTCGCCGTCCCTTGATGAAGCAAAACTGAAGACACCGTACTCTTCTACC
ATCTATGACAAAAACGGAAAAGAAATCGCCGAAATCGGTTCGGAAAAACGGACGTACGTGTCGATTAAAGACATCCCGGA
TTCCGTCAAAAACGCATTTCTTGCAACGGAAGACGCCCGTTTCTATGACCACCACGGCGTCGACCCGATCCGGATCGGAG
GAGCGCTTCTGGCAAACTTCGAAGGCGGATTTGGTTCTGAAGGCGGAAGTACGATCACACAGCAGGTCGTCAAAAACTCC
CTTCTGTCACATGAAAAAACGCTGAAACGGAAAGTCCAGGAAGTCTGGCTGTCTTTCCAGCTGGAAAGAAAATACTCAAA
AGACGAAATTCTTGAAATGTATTTAAATCGGATTTACTTCTCGCCGCAGGCCTACGGTGTCGGAAAAGCGGCTGAGCAGT
TCTACGGGGTTACAGATCTAAACGACTTAACGGTCGAGCAGGCCGCAACGCTTGCCGGCATGCCGCAAAGCCCGAATAAC
TACAACCCGATCAAACATCCGGAAAAAGCCGAGAAACGGCGCAATGTCGTGCTGAGCCTGATGAATAAACACGGCTTTAT
CTCTGACGCAGAGTACAACAAAGCGAAAAAAGTAGCCGTCACAAAAGGCCTTGTATCTCCGAAAGAGTACGCAAAAACAA
CGTCCAACAAATACAGCGCGTTTATCGAGCAGGCCGTCGCCGAAGTGAAAGAAAAAGCGAACGTCACTCCGGGAACGGAC
GGACTGAAGATTTACACGACGATTGATACAGACGCACAGGATTATGTGGATGAGCTGATGGACGGCGATTCTATTCAGTA
TACCGAAAAAATGCAGGCCGGCCTCACCCTGCTCGATACGAAAACCGGAGAGATCCGCGCGCTCGGCGGCGGACGCGACC
GGAAAGCAGGCGACTTTAACTATGCAGTAGATACTAAACGTCAACCCGGTTCAACGATTAAGCCAATCCTTGACTACGGC
CCTGTCATTGAGAACAAGAAATGGTCGACGTATGAACAGATTAAAGATGAAGCATACACGTATTCAACTGGAGATCCGAT
TAACAACTATGACAGAAGCTACAGAGGCTGGATATCGATGAGAGAGGCGCTGGTCGATTCTCGAAACATCCCGGCTTTAA
AAGCGTTCCAGGCAGCCGGCAAAGACAATATTGTAAAATTTGCAGGCAACCTTGGCCTTAACATAAATTCAGATGACTTA
GTGGAAGCTTATGCAATCGGCGGCTTCGGAAATGGTGTGTCGCCTCTGCAAATGGCCGGAGCGTACAGCGCCTTCGGCAA
CAACGGCTACTACAATGAGCCGCATACTGTTACCGCGGTGGAATTCAATGACGGCACTAAGCTTGATTTGACGCCTGAAT
CAAAGGCTGCCATGAGCGATTATACCGCATTTATGATAACGGATATGCTGAAAAGCGCTGTTCAGCGAGGAACAGGAACA
GCGGCTCAAGTCCCCGGCGTCACTGTAGCAGGTAAAACGGGTACGACGAACTTTACCGAGGACGACATTAGAAAACACGG
CATAAATCGAAACGGAGCGAGAGATTCATGGTTCGTCGGCTATACGCCGCAATATACCGCAGCGGTCTGGACCGGTAAAG
AAGGCTTTAATTCGCTGAGCCAGAGCGAACAGCAAGTCGCCAAGCTCCTATTCAAAAAACTGATTGCCAAGGTGGACAAC
GGCAGCGGCTCATTTGAAAAGCCTGACAGCGTCGTTGAAGCGACCGTTCTAAAAGGCTCGAACCCGCCGGTGCTCGCCAG
CAGCAACACGCCTAGCGATAAAAAAGTCACTGAGTACTTTGTAAAAGGCACACAGCCGACGACCGTTTCGAAAAAATATG
AAGAAAAAGAAAATGCCGACAAGCCGTCAGGTTTGAGCGCAGAGTATGATGAAGCATCAAAGAGCATTAAGCTTTCATGG
TCATATTCCGGAGACGGTGATGTATCCTTTAAAGTCAAACAGTCTGTTGACGGAGGCGGATACAGCGAAATTCAAAACAG
CAGTGCGAAAGAAGCCGTCATACCGAATGCAAAAGAAGGATCCGTTTACAGATTCCAAGTCACTGCCGTTACCGAAGATG
GTGAAAGCGACCCTGCTTCTGTAACGCTTAAAGTCAACGCGGCTGATGAGGATGAGTCAAAAACGGACGATGAAAAGAAA
GACGATGACGAGAAGAAGCAGGATGCTGATGATGATCAGGACAAAGACAAACAAAATAACGGCAATTCAAATCAGCCGAA
TGATCAAAATCCAGGAAATTCCGATCAAAACAATCAAAATCCGGGAAATTCAGACCAAAACAACGGCAGAAAAGATGATG
ACGCCGATGATCAAGACAACGATCAAAATAAAGATAAAGATAAAGACAAAAACAAAAACCCGGATCAAGGCGCCAACACA
GGTGATCAACAAACAAATTCCAACGGTCAATCAACCGGCACCAATTGA

Upstream 100 bases:

>100_bases
TTATATTAAGATTATTGATCAGTTGTATTTTTCGCCATCATAAATTATGATTTTAAAGTCATGTGAGCGGAAAGGTTTAA
CTACGAAAGGTTGAGATGTT

Downstream 100 bases:

>100_bases
TCAAAAAAGCCACTCACAATTTAGTGAGTGGCTTTTTTATGATTCATGGCAACATATTTATAAAATTGCTTTTTCATTTC
CGTAAACAATTCGTCAAGCT

Product: PonA

Products: NA

Alternate protein names: PBP1; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 895; Mature: 894

Protein sequence:

>895_residues
MSDQFNSRQERRKAQQGKSRSNTHSKPKKKKKAGLFKKILLSILIIGVIGLIAGGVTFAVMVADSPSLDEAKLKTPYSST
IYDKNGKEIAEIGSEKRTYVSIKDIPDSVKNAFLATEDARFYDHHGVDPIRIGGALLANFEGGFGSEGGSTITQQVVKNS
LLSHEKTLKRKVQEVWLSFQLERKYSKDEILEMYLNRIYFSPQAYGVGKAAEQFYGVTDLNDLTVEQAATLAGMPQSPNN
YNPIKHPEKAEKRRNVVLSLMNKHGFISDAEYNKAKKVAVTKGLVSPKEYAKTTSNKYSAFIEQAVAEVKEKANVTPGTD
GLKIYTTIDTDAQDYVDELMDGDSIQYTEKMQAGLTLLDTKTGEIRALGGGRDRKAGDFNYAVDTKRQPGSTIKPILDYG
PVIENKKWSTYEQIKDEAYTYSTGDPINNYDRSYRGWISMREALVDSRNIPALKAFQAAGKDNIVKFAGNLGLNINSDDL
VEAYAIGGFGNGVSPLQMAGAYSAFGNNGYYNEPHTVTAVEFNDGTKLDLTPESKAAMSDYTAFMITDMLKSAVQRGTGT
AAQVPGVTVAGKTGTTNFTEDDIRKHGINRNGARDSWFVGYTPQYTAAVWTGKEGFNSLSQSEQQVAKLLFKKLIAKVDN
GSGSFEKPDSVVEATVLKGSNPPVLASSNTPSDKKVTEYFVKGTQPTTVSKKYEEKENADKPSGLSAEYDEASKSIKLSW
SYSGDGDVSFKVKQSVDGGGYSEIQNSSAKEAVIPNAKEGSVYRFQVTAVTEDGESDPASVTLKVNAADEDESKTDDEKK
DDDEKKQDADDDQDKDKQNNGNSNQPNDQNPGNSDQNNQNPGNSDQNNGRKDDDADDQDNDQNKDKDKDKNKNPDQGANT
GDQQTNSNGQSTGTN

Sequences:

>Translated_895_residues
MSDQFNSRQERRKAQQGKSRSNTHSKPKKKKKAGLFKKILLSILIIGVIGLIAGGVTFAVMVADSPSLDEAKLKTPYSST
IYDKNGKEIAEIGSEKRTYVSIKDIPDSVKNAFLATEDARFYDHHGVDPIRIGGALLANFEGGFGSEGGSTITQQVVKNS
LLSHEKTLKRKVQEVWLSFQLERKYSKDEILEMYLNRIYFSPQAYGVGKAAEQFYGVTDLNDLTVEQAATLAGMPQSPNN
YNPIKHPEKAEKRRNVVLSLMNKHGFISDAEYNKAKKVAVTKGLVSPKEYAKTTSNKYSAFIEQAVAEVKEKANVTPGTD
GLKIYTTIDTDAQDYVDELMDGDSIQYTEKMQAGLTLLDTKTGEIRALGGGRDRKAGDFNYAVDTKRQPGSTIKPILDYG
PVIENKKWSTYEQIKDEAYTYSTGDPINNYDRSYRGWISMREALVDSRNIPALKAFQAAGKDNIVKFAGNLGLNINSDDL
VEAYAIGGFGNGVSPLQMAGAYSAFGNNGYYNEPHTVTAVEFNDGTKLDLTPESKAAMSDYTAFMITDMLKSAVQRGTGT
AAQVPGVTVAGKTGTTNFTEDDIRKHGINRNGARDSWFVGYTPQYTAAVWTGKEGFNSLSQSEQQVAKLLFKKLIAKVDN
GSGSFEKPDSVVEATVLKGSNPPVLASSNTPSDKKVTEYFVKGTQPTTVSKKYEEKENADKPSGLSAEYDEASKSIKLSW
SYSGDGDVSFKVKQSVDGGGYSEIQNSSAKEAVIPNAKEGSVYRFQVTAVTEDGESDPASVTLKVNAADEDESKTDDEKK
DDDEKKQDADDDQDKDKQNNGNSNQPNDQNPGNSDQNNQNPGNSDQNNGRKDDDADDQDNDQNKDKDKDKNKNPDQGANT
GDQQTNSNGQSTGTN
>Mature_894_residues
SDQFNSRQERRKAQQGKSRSNTHSKPKKKKKAGLFKKILLSILIIGVIGLIAGGVTFAVMVADSPSLDEAKLKTPYSSTI
YDKNGKEIAEIGSEKRTYVSIKDIPDSVKNAFLATEDARFYDHHGVDPIRIGGALLANFEGGFGSEGGSTITQQVVKNSL
LSHEKTLKRKVQEVWLSFQLERKYSKDEILEMYLNRIYFSPQAYGVGKAAEQFYGVTDLNDLTVEQAATLAGMPQSPNNY
NPIKHPEKAEKRRNVVLSLMNKHGFISDAEYNKAKKVAVTKGLVSPKEYAKTTSNKYSAFIEQAVAEVKEKANVTPGTDG
LKIYTTIDTDAQDYVDELMDGDSIQYTEKMQAGLTLLDTKTGEIRALGGGRDRKAGDFNYAVDTKRQPGSTIKPILDYGP
VIENKKWSTYEQIKDEAYTYSTGDPINNYDRSYRGWISMREALVDSRNIPALKAFQAAGKDNIVKFAGNLGLNINSDDLV
EAYAIGGFGNGVSPLQMAGAYSAFGNNGYYNEPHTVTAVEFNDGTKLDLTPESKAAMSDYTAFMITDMLKSAVQRGTGTA
AQVPGVTVAGKTGTTNFTEDDIRKHGINRNGARDSWFVGYTPQYTAAVWTGKEGFNSLSQSEQQVAKLLFKKLIAKVDNG
SGSFEKPDSVVEATVLKGSNPPVLASSNTPSDKKVTEYFVKGTQPTTVSKKYEEKENADKPSGLSAEYDEASKSIKLSWS
YSGDGDVSFKVKQSVDGGGYSEIQNSSAKEAVIPNAKEGSVYRFQVTAVTEDGESDPASVTLKVNAADEDESKTDDEKKD
DDEKKQDADDDQDKDKQNNGNSNQPNDQNPGNSDQNNQNPGNSDQNNGRKDDDADDQDNDQNKDKDKDKNKNPDQGANTG
DQQTNSNGQSTGTN

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass type II membrane protein. Note=Probably found all over the whole cell at low concentrations. Also localizes to the division site in vegetative cells [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 fibronectin type-III domain [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=317, Percent_Identity=36.2776025236593, Blast_Score=206, Evalue=5e-54,
Organism=Escherichia coli, GI1786343, Length=550, Percent_Identity=28.3636363636364, Blast_Score=195, Evalue=8e-51,
Organism=Escherichia coli, GI1788867, Length=630, Percent_Identity=26.6666666666667, Blast_Score=177, Evalue=3e-45,
Organism=Escherichia coli, GI1789601, Length=140, Percent_Identity=34.2857142857143, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR008957
- InterPro:   IPR003961
- InterPro:   IPR001264
- InterPro:   IPR013783
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00041 fn3; PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.-

Molecular weight: Translated: 97813; Mature: 97682

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS50853 FN3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDQFNSRQERRKAQQGKSRSNTHSKPKKKKKAGLFKKILLSILIIGVIGLIAGGVTFAV
CCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
MVADSPSLDEAKLKTPYSSTIYDKNGKEIAEIGSEKRTYVSIKDIPDSVKNAFLATEDAR
EEECCCCCCCHHCCCCCCCCEECCCCHHHHHCCCCCCEEEEECCCCHHHHHHEECCCCCH
FYDHHGVDPIRIGGALLANFEGGFGSEGGSTITQQVVKNSLLSHEKTLKRKVQEVWLSFQ
HHHCCCCCEEEECCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LERKYSKDEILEMYLNRIYFSPQAYGVGKAAEQFYGVTDLNDLTVEQAATLAGMPQSPNN
HHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCC
YNPIKHPEKAEKRRNVVLSLMNKHGFISDAEYNKAKKVAVTKGLVSPKEYAKTTSNKYSA
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHCCHHHHH
FIEQAVAEVKEKANVTPGTDGLKIYTTIDTDAQDYVDELMDGDSIQYTEKMQAGLTLLDT
HHHHHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHHHHHHCCCCCHHHHHHHCCEEEEEC
KTGEIRALGGGRDRKAGDFNYAVDTKRQPGSTIKPILDYGPVIENKKWSTYEQIKDEAYT
CCCCEEEECCCCCCCCCCCCEEECCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHCEE
YSTGDPINNYDRSYRGWISMREALVDSRNIPALKAFQAAGKDNIVKFAGNLGLNINSDDL
ECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCEEEEECCCCCCCCCHHH
VEAYAIGGFGNGVSPLQMAGAYSAFGNNGYYNEPHTVTAVEFNDGTKLDLTPESKAAMSD
HHHEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCEEEECCCHHHHHHH
YTAFMITDMLKSAVQRGTGTAAQVPGVTVAGKTGTTNFTEDDIRKHGINRNGARDSWFVG
HHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCHHHHHHCCCCCCCCCCCEEEC
YTPQYTAAVWTGKEGFNSLSQSEQQVAKLLFKKLIAKVDNGSGSFEKPDSVVEATVLKGS
CCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHEECCC
NPPVLASSNTPSDKKVTEYFVKGTQPTTVSKKYEEKENADKPSGLSAEYDEASKSIKLSW
CCCEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHCCCEEEEEE
SYSGDGDVSFKVKQSVDGGGYSEIQNSSAKEAVIPNAKEGSVYRFQVTAVTEDGESDPAS
ECCCCCCEEEEEECCCCCCCHHHHCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCE
VTLKVNAADEDESKTDDEKKDDDEKKQDADDDQDKDKQNNGNSNQPNDQNPGNSDQNNQN
EEEEEECCCCCCCCCCCCCCCCHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCC
PGNSDQNNGRKDDDADDQDNDQNKDKDKDKNKNPDQGANTGDQQTNSNGQSTGTN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SDQFNSRQERRKAQQGKSRSNTHSKPKKKKKAGLFKKILLSILIIGVIGLIAGGVTFAV
CCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
MVADSPSLDEAKLKTPYSSTIYDKNGKEIAEIGSEKRTYVSIKDIPDSVKNAFLATEDAR
EEECCCCCCCHHCCCCCCCCEECCCCHHHHHCCCCCCEEEEECCCCHHHHHHEECCCCCH
FYDHHGVDPIRIGGALLANFEGGFGSEGGSTITQQVVKNSLLSHEKTLKRKVQEVWLSFQ
HHHCCCCCEEEECCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LERKYSKDEILEMYLNRIYFSPQAYGVGKAAEQFYGVTDLNDLTVEQAATLAGMPQSPNN
HHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCC
YNPIKHPEKAEKRRNVVLSLMNKHGFISDAEYNKAKKVAVTKGLVSPKEYAKTTSNKYSA
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHCCHHHHH
FIEQAVAEVKEKANVTPGTDGLKIYTTIDTDAQDYVDELMDGDSIQYTEKMQAGLTLLDT
HHHHHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHHHHHHCCCCCHHHHHHHCCEEEEEC
KTGEIRALGGGRDRKAGDFNYAVDTKRQPGSTIKPILDYGPVIENKKWSTYEQIKDEAYT
CCCCEEEECCCCCCCCCCCCEEECCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHCEE
YSTGDPINNYDRSYRGWISMREALVDSRNIPALKAFQAAGKDNIVKFAGNLGLNINSDDL
ECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCEEEEECCCCCCCCCHHH
VEAYAIGGFGNGVSPLQMAGAYSAFGNNGYYNEPHTVTAVEFNDGTKLDLTPESKAAMSD
HHHEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCEEEECCCHHHHHHH
YTAFMITDMLKSAVQRGTGTAAQVPGVTVAGKTGTTNFTEDDIRKHGINRNGARDSWFVG
HHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCHHHHHHCCCCCCCCCCCEEEC
YTPQYTAAVWTGKEGFNSLSQSEQQVAKLLFKKLIAKVDNGSGSFEKPDSVVEATVLKGS
CCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHEECCC
NPPVLASSNTPSDKKVTEYFVKGTQPTTVSKKYEEKENADKPSGLSAEYDEASKSIKLSW
CCCEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHCCCEEEEEE
SYSGDGDVSFKVKQSVDGGGYSEIQNSSAKEAVIPNAKEGSVYRFQVTAVTEDGESDPAS
ECCCCCCEEEEEECCCCCCCHHHHCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCE
VTLKVNAADEDESKTDDEKKDDDEKKQDADDDQDKDKQNNGNSNQPNDQNPGNSDQNNQN
EEEEEECCCCCCCCCCCCCCCCHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCC
PGNSDQNNGRKDDDADDQDNDQNKDKDKDKNKNPDQGANTGDQQTNSNGQSTGTN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7814321; 8760912; 9384377; 9721295; 10322023 [H]