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The map label for this gene is dfrA [H]

Identifier: 52786063

GI number: 52786063

Start: 2269688

End: 2270176

Strand: Reverse

Name: dfrA [H]

Synonym: BLi02319

Alternate gene names: 52786063

Gene position: 2270176-2269688 (Counterclockwise)

Preceding gene: 52786064

Following gene: 52786062

Centisome position: 53.76

GC content: 46.22

Gene sequence:

>489_bases
ATGATCTCATTGATTGCTGCAATGGACAGAAACCGCCTGATCGGCAAAGACAATGATTTGCCATGGCATCTGCCGCAAGA
TTTGAAGTATTTTAAAGAGGTGACGAAAGGGCACGCCGTAATCATGGGGAGAAAGACGTTCGAGTCCATCGGCAGACCTT
TGCCCCACCGCGAGAACATCATCGTCACATCCAATAAAGAACTGGATATCCCGAATTGTCAGGTGATGCACTCGGCAGAG
GAAGCGGTGCGGTTTGCGAAAAACAGAAACGAAGAATGTTTTGTCATCGGCGGTTCAACACTATACACGGAGATTCTGCC
TTTTGCGGACAAGCTGTATGTGACAAAAATTGATGAAACCTTTGAAGGGGACCGGTACTTTCCTGAATTCAGCGAAGCTG
AGTGGGAAATCGTTTCGAGGCGGAAAGGCCTGAAAGATGACAAAAATCCTTATGACTACGAATTCCTCGTTTATCAAAGA
AAAGGATAG

Upstream 100 bases:

>100_bases
AACCTCCATCTTCATGCATCATGTTCACAGATGATCACACAAAAAGATGAGCATCATCCGGCATTATTTCGCTACAAACA
TCATAAGGAGGCATTAAACA

Downstream 100 bases:

>100_bases
AGGAGAGGATTTTCATGTTGCGCTATTGTTTTTTATGGATTTATTCGGCCTATATGCTCATCAAGCATATCAAGCTTCTG
CAGGCGCTGCAGACACTTGA

Product: DfrA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MISLIAAMDRNRLIGKDNDLPWHLPQDLKYFKEVTKGHAVIMGRKTFESIGRPLPHRENIIVTSNKELDIPNCQVMHSAE
EAVRFAKNRNEECFVIGGSTLYTEILPFADKLYVTKIDETFEGDRYFPEFSEAEWEIVSRRKGLKDDKNPYDYEFLVYQR
KG

Sequences:

>Translated_162_residues
MISLIAAMDRNRLIGKDNDLPWHLPQDLKYFKEVTKGHAVIMGRKTFESIGRPLPHRENIIVTSNKELDIPNCQVMHSAE
EAVRFAKNRNEECFVIGGSTLYTEILPFADKLYVTKIDETFEGDRYFPEFSEAEWEIVSRRKGLKDDKNPYDYEFLVYQR
KG
>Mature_162_residues
MISLIAAMDRNRLIGKDNDLPWHLPQDLKYFKEVTKGHAVIMGRKTFESIGRPLPHRENIIVTSNKELDIPNCQVMHSAE
EAVRFAKNRNEECFVIGGSTLYTEILPFADKLYVTKIDETFEGDRYFPEFSEAEWEIVSRRKGLKDDKNPYDYEFLVYQR
KG

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=184, Percent_Identity=32.0652173913043, Blast_Score=78, Evalue=3e-15,
Organism=Homo sapiens, GI28827793, Length=169, Percent_Identity=31.3609467455621, Blast_Score=67, Evalue=7e-12,
Organism=Escherichia coli, GI1786233, Length=161, Percent_Identity=44.7204968944099, Blast_Score=131, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17505899, Length=155, Percent_Identity=33.5483870967742, Blast_Score=69, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6324810, Length=137, Percent_Identity=34.3065693430657, Blast_Score=74, Evalue=9e-15,
Organism=Drosophila melanogaster, GI116008026, Length=152, Percent_Identity=35.5263157894737, Blast_Score=84, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24647458, Length=152, Percent_Identity=35.5263157894737, Blast_Score=84, Evalue=4e-17,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 18935; Mature: 18935

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISLIAAMDRNRLIGKDNDLPWHLPQDLKYFKEVTKGHAVIMGRKTFESIGRPLPHRENI
CEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECHHHHHHCCCCCCCCCE
IVTSNKELDIPNCQVMHSAEEAVRFAKNRNEECFVIGGSTLYTEILPFADKLYVTKIDET
EEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHCCCCEEEEEECCCC
FEGDRYFPEFSEAEWEIVSRRKGLKDDKNPYDYEFLVYQRKG
CCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCEEEEEEEECC
>Mature Secondary Structure
MISLIAAMDRNRLIGKDNDLPWHLPQDLKYFKEVTKGHAVIMGRKTFESIGRPLPHRENI
CEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECHHHHHHCCCCCCCCCE
IVTSNKELDIPNCQVMHSAEEAVRFAKNRNEECFVIGGSTLYTEILPFADKLYVTKIDET
EEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHCCCCEEEEEECCCC
FEGDRYFPEFSEAEWEIVSRRKGLKDDKNPYDYEFLVYQRKG
CCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840350; 9384377 [H]