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The map label for this gene is ilvA [H]

Identifier: 52786059

GI number: 52786059

Start: 2265849

End: 2267117

Strand: Reverse

Name: ilvA [H]

Synonym: BLi02315

Alternate gene names: 52786059

Gene position: 2267117-2265849 (Counterclockwise)

Preceding gene: 52786062

Following gene: 52786058

Centisome position: 53.69

GC content: 47.2

Gene sequence:

>1269_bases
ATGAAACCGTTGCTTAAAGAAAACTCTCTCATCCAAGTCAAAGACATTTTAAAAGCCCACCAAAATGTAAAAGATGTTGT
TATTCATACCCCTCTCCAAAAAAATGAGAGGCTGTCCGAGAGGTACGATTGCAACGTATATTTAAAAAGAGAAGACCTGC
AGGTTGTCCGCTCTTTTAAACTGAGAGGCGCCTATTATAAAATGAAGCAGCTTTCAAAAGAAACGACGAAAAACGGGGTG
GTCTGCGCCAGTGCAGGAAACCATGCGCAGGGAGTCGCTTTTTCCTGCAAACATCTCGGTATTCACGGAAAGATTTTTAT
GCCGTCGACGACCCCGAGGCAAAAAATTTCCCAAGTCGAATTGTTCGGCAAGGAATACGTCGAGATCATCCTGACAGGCG
ACACGTTTGATGATGCTTATCAAAGCGCAGTCGCCTGCTGCGAGGAAGAAAAGCGCGCATTTATCCACCCGTTTGACGAT
CCTGCCGTCATGGCTGGCCAGGGTACGGTCGCGGTCGAAATCTTAAACGATATCGAAACGGAACCCCATTATTTATTCGC
AAGCGTAGGCGGAGGGGGTCTGCTTTCAGGAGTGGGAACCTATATGAAAAACGTGTCCCCGGATACAAAGCTGATCGCGG
TTGAGCCTAAAGGCGCGCCGGCTCTTTTTGAATCAAACAAAAAAGGCGAAGTCGTCATCCTTGAGAAAATCGATAAATTT
GTCGATGGAGCCGCTGTGCAGAAAATCGGCGAAGAGACATTTAAAACGCTTGAAACCGTTGTCGATGATATTTTGCTCGT
TCCTGAAGGAAAGGTGTGCACCGCGATTCTTGAGCTGTACAACCAATGCGCGATTGTCGCCGAACCTGCGGGAGCGCTGC
CGATCGCCGCTTTAGATCTTTACAGGGATGAAATTAAAGGGAAAAATGTCGTATGCATTGTGAGCGGAGGAAACAACGAT
ATCGGCAGAATGCAGGAAATCAAAGACAGATCGATGATGTATGAAGGACTCCAGCATTATTTCATCGTGAACTTCCCGCA
AAGGGCGGGAGCTTTGCGCGAATTTTTAGATGAAGTGCTCGGGCCGACCGACGATATCACCCGGTTTGAATATACGAAGA
AAAATAATAAAAGCAGCGGCCCTGCGCTCGTCGGCATCGAATTGAAGCAGCGCGAAGATTACGAGCCGCTGATCGCAAGA
ATGGACAGGAAAGGCTTCCGCTATGTCGAAGTCAATAAAGATGAAGGGCTCTTTCATTTGCTGATTTAA

Upstream 100 bases:

>100_bases
TATAATATTCTGTAAATAACAAAGAATTGCCCTTTAAATTTTGAATATTCATGATATAATGGCGTATTAAATAAAGCTTT
ATACAGAAAGGATCTTATAC

Downstream 100 bases:

>100_bases
GAAATTTTTTTGAAAACAAACCTAAATCACAGACAACGGCTTCAGAGATTATGTATAATAAAGAAAATATCTCATAAATG
AGGATTGATAGCTTGTTACT

Product: threonine dehydratase

Products: NA

Alternate protein names: Threonine deaminase [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MKPLLKENSLIQVKDILKAHQNVKDVVIHTPLQKNERLSERYDCNVYLKREDLQVVRSFKLRGAYYKMKQLSKETTKNGV
VCASAGNHAQGVAFSCKHLGIHGKIFMPSTTPRQKISQVELFGKEYVEIILTGDTFDDAYQSAVACCEEEKRAFIHPFDD
PAVMAGQGTVAVEILNDIETEPHYLFASVGGGGLLSGVGTYMKNVSPDTKLIAVEPKGAPALFESNKKGEVVILEKIDKF
VDGAAVQKIGEETFKTLETVVDDILLVPEGKVCTAILELYNQCAIVAEPAGALPIAALDLYRDEIKGKNVVCIVSGGNND
IGRMQEIKDRSMMYEGLQHYFIVNFPQRAGALREFLDEVLGPTDDITRFEYTKKNNKSSGPALVGIELKQREDYEPLIAR
MDRKGFRYVEVNKDEGLFHLLI

Sequences:

>Translated_422_residues
MKPLLKENSLIQVKDILKAHQNVKDVVIHTPLQKNERLSERYDCNVYLKREDLQVVRSFKLRGAYYKMKQLSKETTKNGV
VCASAGNHAQGVAFSCKHLGIHGKIFMPSTTPRQKISQVELFGKEYVEIILTGDTFDDAYQSAVACCEEEKRAFIHPFDD
PAVMAGQGTVAVEILNDIETEPHYLFASVGGGGLLSGVGTYMKNVSPDTKLIAVEPKGAPALFESNKKGEVVILEKIDKF
VDGAAVQKIGEETFKTLETVVDDILLVPEGKVCTAILELYNQCAIVAEPAGALPIAALDLYRDEIKGKNVVCIVSGGNND
IGRMQEIKDRSMMYEGLQHYFIVNFPQRAGALREFLDEVLGPTDDITRFEYTKKNNKSSGPALVGIELKQREDYEPLIAR
MDRKGFRYVEVNKDEGLFHLLI
>Mature_422_residues
MKPLLKENSLIQVKDILKAHQNVKDVVIHTPLQKNERLSERYDCNVYLKREDLQVVRSFKLRGAYYKMKQLSKETTKNGV
VCASAGNHAQGVAFSCKHLGIHGKIFMPSTTPRQKISQVELFGKEYVEIILTGDTFDDAYQSAVACCEEEKRAFIHPFDD
PAVMAGQGTVAVEILNDIETEPHYLFASVGGGGLLSGVGTYMKNVSPDTKLIAVEPKGAPALFESNKKGEVVILEKIDKF
VDGAAVQKIGEETFKTLETVVDDILLVPEGKVCTAILELYNQCAIVAEPAGALPIAALDLYRDEIKGKNVVCIVSGGNND
IGRMQEIKDRSMMYEGLQHYFIVNFPQRAGALREFLDEVLGPTDDITRFEYTKKNNKSSGPALVGIELKQREDYEPLIAR
MDRKGFRYVEVNKDEGLFHLLI

Specific function: Catalyzes the formation of alpha-ketobutyrate from threonine in a two-step reaction. The first step is a dehydration of threonine, followed by rehydration and liberation of ammonia [H]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the serine/threonine dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI11345492, Length=318, Percent_Identity=30.8176100628931, Blast_Score=145, Evalue=6e-35,
Organism=Homo sapiens, GI33469958, Length=322, Percent_Identity=27.9503105590062, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI19923959, Length=310, Percent_Identity=28.0645161290323, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1790207, Length=389, Percent_Identity=36.5038560411311, Blast_Score=221, Evalue=9e-59,
Organism=Escherichia coli, GI1789505, Length=322, Percent_Identity=35.4037267080745, Blast_Score=177, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI71991565, Length=349, Percent_Identity=32.0916905444126, Blast_Score=158, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17537387, Length=387, Percent_Identity=31.5245478036176, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17508781, Length=315, Percent_Identity=26.984126984127, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6320930, Length=428, Percent_Identity=34.8130841121495, Blast_Score=224, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6322631, Length=309, Percent_Identity=35.5987055016181, Blast_Score=160, Evalue=5e-40,
Organism=Saccharomyces cerevisiae, GI6319788, Length=291, Percent_Identity=24.7422680412371, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI21355833, Length=346, Percent_Identity=32.0809248554913, Blast_Score=138, Evalue=8e-33,
Organism=Drosophila melanogaster, GI24645328, Length=250, Percent_Identity=30.8, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR001721
- InterPro:   IPR011820 [H]

Pfam domain/function: PF00291 PALP; PF00585 Thr_dehydrat_C [H]

EC number: =4.3.1.19 [H]

Molecular weight: Translated: 46967; Mature: 46967

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPLLKENSLIQVKDILKAHQNVKDVVIHTPLQKNERLSERYDCNVYLKREDLQVVRSFK
CCCCCCCCCEEHHHHHHHHHCCCCEEEEECCCCCCCHHHHHCCCEEEEEHHHHHHHHHHH
LRGAYYKMKQLSKETTKNGVVCASAGNHAQGVAFSCKHLGIHGKIFMPSTTPRQKISQVE
HHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEECCCEEEEECCCCCHHHHHHHHH
LFGKEYVEIILTGDTFDDAYQSAVACCEEEKRAFIHPFDDPAVMAGQGTVAVEILNDIET
HHCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCEECCCCCCCEEECCCCEEEEEHHHCCC
EPHYLFASVGGGGLLSGVGTYMKNVSPDTKLIAVEPKGAPALFESNKKGEVVILEKIDKF
CCCEEEEECCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHCCCCCCCEEEHHHHHHH
VDGAAVQKIGEETFKTLETVVDDILLVPEGKVCTAILELYNQCAIVAEPAGALPIAALDL
HCCHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHCCEEEECCCCCCCHHHHHH
YRDEIKGKNVVCIVSGGNNDIGRMQEIKDRSMMYEGLQHYFIVNFPQRAGALREFLDEVL
HHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHC
GPTDDITRFEYTKKNNKSSGPALVGIELKQREDYEPLIARMDRKGFRYVEVNKDEGLFHL
CCCCCCCCEEEECCCCCCCCCEEEEEEECCCCCCCHHHHHHCCCCCEEEEECCCCCEEEE
LI
EC
>Mature Secondary Structure
MKPLLKENSLIQVKDILKAHQNVKDVVIHTPLQKNERLSERYDCNVYLKREDLQVVRSFK
CCCCCCCCCEEHHHHHHHHHCCCCEEEEECCCCCCCHHHHHCCCEEEEEHHHHHHHHHHH
LRGAYYKMKQLSKETTKNGVVCASAGNHAQGVAFSCKHLGIHGKIFMPSTTPRQKISQVE
HHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEECCCEEEEECCCCCHHHHHHHHH
LFGKEYVEIILTGDTFDDAYQSAVACCEEEKRAFIHPFDDPAVMAGQGTVAVEILNDIET
HHCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCEECCCCCCCEEECCCCEEEEEHHHCCC
EPHYLFASVGGGGLLSGVGTYMKNVSPDTKLIAVEPKGAPALFESNKKGEVVILEKIDKF
CCCEEEEECCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHCCCCCCCEEEHHHHHHH
VDGAAVQKIGEETFKTLETVVDDILLVPEGKVCTAILELYNQCAIVAEPAGALPIAALDL
HCCHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHCCEEEECCCCCCCHHHHHH
YRDEIKGKNVVCIVSGGNNDIGRMQEIKDRSMMYEGLQHYFIVNFPQRAGALREFLDEVL
HHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHC
GPTDDITRFEYTKKNNKSSGPALVGIELKQREDYEPLIARMDRKGFRYVEVNKDEGLFHL
CCCCCCCCEEEECCCCCCCCCEEEEEEECCCCCCCHHHHHHCCCCCEEEEECCCCCEEEE
LI
EC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]