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The map label for this gene is wcaL [C]

Identifier: 52785956

GI number: 52785956

Start: 2166901

End: 2168109

Strand: Reverse

Name: wcaL [C]

Synonym: BLi02208

Alternate gene names: 52785956

Gene position: 2168109-2166901 (Counterclockwise)

Preceding gene: 52785957

Following gene: 52785955

Centisome position: 51.34

GC content: 43.76

Gene sequence:

>1209_bases
ATGAAAATTCTTCTTGCCACTTATTGGAGTATTCCGCACGTAGGTGGTGTGTGGACATATATGGTCCAGCTGAAAGAATC
GCTTGAATCTATGGGACATGAAGTCGATCTGCTTGGATATGGAGAAGAGAACGAGATTGTTCATATTGTAAACGAAGATC
GCAAGGTGAAAAAAGAACAGCTTTTGCCTATGATTGAGCAATCAGTCGATCAACAGTCTTTTCCGCAATTATATGACAAT
CAATTGGTCCACTTTACTGAGATCCAGCGCTATGTATACGAACTTGCAGCTATTTATTTAGGGCTGGAAAAATACGACCT
GATCCATGCCCAAGATGTCATCTCAAGCGGCTGCCTGAAAAGAGTGAAGCAAAAAGACACGCCGCTTGTTGCCACGCTTC
ACGGGAGTGTAGCCCATGAAATCAGACATCAGCTTACAACGATCCACAGGAAGTCTAATTCTGACATCGCAAGGGCGTAC
TTTGACCATCTTGAACGGGTCGGCGCTGTTTCGGCTGACGCTACAATTGTTGCCAACAATTGGTTGAAGAATCTGTTGAC
AAAGGAATTCGATACACCGGAGGAACAAATCAGCGTTCTCCATTATGGCTATGATATTGACAGCTTTCTAAAACGAAAGG
ATACAAAGTCAGCGGAAATACCGCAAACAGATAAGAAAATTATCCTTTTCACAGGAAGGCTCGTTGAAAACAAAGGTGTT
CAGCATCTGCTTGCCGCACTTTCGCATTTAAAAGAGGTTCGGGATGATTGGGTATGCTGGATTGCCGGCGACGGGGAGAG
AATGGCGGGACTGAGAATTTTATGCGGGCAGCTCGGTTTGGAAAACGATGTCGTGTTTATGAAAAAGCGCGATGATGTCC
CATATCTTCTGTCAATCGCTGATGTGTATGTTTTGCCGAGTCTTCTCGAAAATCAGCCGCTGTCTGTTATTGAAGCGCAA
TTAGCCGGACTTCCTATTATCGTCAGCGATGCAGGGGGACTTCCCGAAATCGTTGAACATAATGTGACCGGTATGGTGAC
GCCTAAAGGAGATGCACAGGCAATTTGCAACTCTATCAATCAGTTGTTGGAAGATGAGACGCTTAGAAAAACTTTAGGGT
CCAATGCACACAAATTTGCAATGGAGTATTGGAATATGGATGAAGCTGTCAACAAGGTTTTGGACATTTATCAACAAACT
TTAAAATAA

Upstream 100 bases:

>100_bases
AAAATAATCTTTCTTAATTGGATATCCGTCCTACACAAGATATAGCAATCACCAATATAAATAATTAGAGCATTGGATAG
AAATGAAAAGGGAGATGCTT

Downstream 100 bases:

>100_bases
AGGAGACATGTCGTATGAAGTCTGAAATTAAAAAGCATTTGTTTGAACTTCTGACAAAAGAGGAAATCGATGTCGGTTGG
TGGGATAAATATTTGGAATT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MKILLATYWSIPHVGGVWTYMVQLKESLESMGHEVDLLGYGEENEIVHIVNEDRKVKKEQLLPMIEQSVDQQSFPQLYDN
QLVHFTEIQRYVYELAAIYLGLEKYDLIHAQDVISSGCLKRVKQKDTPLVATLHGSVAHEIRHQLTTIHRKSNSDIARAY
FDHLERVGAVSADATIVANNWLKNLLTKEFDTPEEQISVLHYGYDIDSFLKRKDTKSAEIPQTDKKIILFTGRLVENKGV
QHLLAALSHLKEVRDDWVCWIAGDGERMAGLRILCGQLGLENDVVFMKKRDDVPYLLSIADVYVLPSLLENQPLSVIEAQ
LAGLPIIVSDAGGLPEIVEHNVTGMVTPKGDAQAICNSINQLLEDETLRKTLGSNAHKFAMEYWNMDEAVNKVLDIYQQT
LK

Sequences:

>Translated_402_residues
MKILLATYWSIPHVGGVWTYMVQLKESLESMGHEVDLLGYGEENEIVHIVNEDRKVKKEQLLPMIEQSVDQQSFPQLYDN
QLVHFTEIQRYVYELAAIYLGLEKYDLIHAQDVISSGCLKRVKQKDTPLVATLHGSVAHEIRHQLTTIHRKSNSDIARAY
FDHLERVGAVSADATIVANNWLKNLLTKEFDTPEEQISVLHYGYDIDSFLKRKDTKSAEIPQTDKKIILFTGRLVENKGV
QHLLAALSHLKEVRDDWVCWIAGDGERMAGLRILCGQLGLENDVVFMKKRDDVPYLLSIADVYVLPSLLENQPLSVIEAQ
LAGLPIIVSDAGGLPEIVEHNVTGMVTPKGDAQAICNSINQLLEDETLRKTLGSNAHKFAMEYWNMDEAVNKVLDIYQQT
LK
>Mature_402_residues
MKILLATYWSIPHVGGVWTYMVQLKESLESMGHEVDLLGYGEENEIVHIVNEDRKVKKEQLLPMIEQSVDQQSFPQLYDN
QLVHFTEIQRYVYELAAIYLGLEKYDLIHAQDVISSGCLKRVKQKDTPLVATLHGSVAHEIRHQLTTIHRKSNSDIARAY
FDHLERVGAVSADATIVANNWLKNLLTKEFDTPEEQISVLHYGYDIDSFLKRKDTKSAEIPQTDKKIILFTGRLVENKGV
QHLLAALSHLKEVRDDWVCWIAGDGERMAGLRILCGQLGLENDVVFMKKRDDVPYLLSIADVYVLPSLLENQPLSVIEAQ
LAGLPIIVSDAGGLPEIVEHNVTGMVTPKGDAQAICNSINQLLEDETLRKTLGSNAHKFAMEYWNMDEAVNKVLDIYQQT
LK

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1788357, Length=184, Percent_Identity=32.0652173913043, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR013534 [H]

Pfam domain/function: PF08323 Glyco_transf_5; PF00534 Glycos_transf_1 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 45486; Mature: 45486

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILLATYWSIPHVGGVWTYMVQLKESLESMGHEVDLLGYGEENEIVHIVNEDRKVKKEQ
CEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEECCCHHHHHHH
LLPMIEQSVDQQSFPQLYDNQLVHFTEIQRYVYELAAIYLGLEKYDLIHAQDVISSGCLK
HHHHHHHHCCHHHCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVKQKDTPLVATLHGSVAHEIRHQLTTIHRKSNSDIARAYFDHLERVGAVSADATIVANN
HHHHCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEHHH
WLKNLLTKEFDTPEEQISVLHYGYDIDSFLKRKDTKSAEIPQTDKKIILFTGRLVENKGV
HHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCEEEEEECCHHHCCCH
QHLLAALSHLKEVRDDWVCWIAGDGERMAGLRILCGQLGLENDVVFMKKRDDVPYLLSIA
HHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHH
DVYVLPSLLENQPLSVIEAQLAGLPIIVSDAGGLPEIVEHNVTGMVTPKGDAQAICNSIN
HHHHHHHHHCCCCHHHHHHHHCCCCEEEECCCCCHHHHHCCCCEEECCCCHHHHHHHHHH
QLLEDETLRKTLGSNAHKFAMEYWNMDEAVNKVLDIYQQTLK
HHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKILLATYWSIPHVGGVWTYMVQLKESLESMGHEVDLLGYGEENEIVHIVNEDRKVKKEQ
CEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEECCCHHHHHHH
LLPMIEQSVDQQSFPQLYDNQLVHFTEIQRYVYELAAIYLGLEKYDLIHAQDVISSGCLK
HHHHHHHHCCHHHCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVKQKDTPLVATLHGSVAHEIRHQLTTIHRKSNSDIARAYFDHLERVGAVSADATIVANN
HHHHCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEHHH
WLKNLLTKEFDTPEEQISVLHYGYDIDSFLKRKDTKSAEIPQTDKKIILFTGRLVENKGV
HHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCEEEEEECCHHHCCCH
QHLLAALSHLKEVRDDWVCWIAGDGERMAGLRILCGQLGLENDVVFMKKRDDVPYLLSIA
HHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHH
DVYVLPSLLENQPLSVIEAQLAGLPIIVSDAGGLPEIVEHNVTGMVTPKGDAQAICNSIN
HHHHHHHHHCCCCHHHHHHHHCCCCEEEECCCCCHHHHHCCCCEEECCCCHHHHHHHHHH
QLLEDETLRKTLGSNAHKFAMEYWNMDEAVNKVLDIYQQTLK
HHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]