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The map label for this gene is csaA [H]

Identifier: 52785933

GI number: 52785933

Start: 2148417

End: 2148749

Strand: Reverse

Name: csaA [H]

Synonym: BLi02184

Alternate gene names: 52785933

Gene position: 2148749-2148417 (Counterclockwise)

Preceding gene: 52785935

Following gene: 52785931

Centisome position: 50.89

GC content: 48.65

Gene sequence:

>333_bases
ATGGCAACAATCGAAGATTTTTTAAAGCTTGATATTCGGGTTGGAACAGTTTTAAAAGCAGAAGAGTTTCCGGAAGCGAA
GGTACCGGCCATTAAGCTTGAAATCGATTTTGGCGGAGAGATTGGCGTCAAGTGTTCGAGCGCTCAAATCACAAAGCGTT
ATACGCCTGAAAAGCTTGTAGGCCGGCAGGTGACGGCAGTCGTCAATTTTCCGCCGCGGCGGATTGCCGGGTTTAAATCG
GAAGTTCTCGTTCTTGGAGGCGTACCGGAAAAAGGCGATGTTATATTGCTGGCCCCTGATCAAGAAGCGCCCAATGGAAC
GAAGATCAGCTGA

Upstream 100 bases:

>100_bases
TAAAATGCAGCGGAAGTCCAGGGTTTAAACGAATCAAAATACATGATATTCTAGTCATGTCAATATCACCCTTTAGAAAG
CGGCCAAAGGAGCTGAGTTC

Downstream 100 bases:

>100_bases
TACATAAAAGAGAGGCTCCCTCTAAATGAAGGAAGCCTCTCTTTTTTATTTCACCTTGAATTTTTTCGTCAATTCTCGCA
GATCTTCCGCCATCTGCTCA

Product: CsaA

Products: AMP; diphosphate +L-methionyl-tRNAMet [C]

Alternate protein names: NA

Number of amino acids: Translated: 110; Mature: 109

Protein sequence:

>110_residues
MATIEDFLKLDIRVGTVLKAEEFPEAKVPAIKLEIDFGGEIGVKCSSAQITKRYTPEKLVGRQVTAVVNFPPRRIAGFKS
EVLVLGGVPEKGDVILLAPDQEAPNGTKIS

Sequences:

>Translated_110_residues
MATIEDFLKLDIRVGTVLKAEEFPEAKVPAIKLEIDFGGEIGVKCSSAQITKRYTPEKLVGRQVTAVVNFPPRRIAGFKS
EVLVLGGVPEKGDVILLAPDQEAPNGTKIS
>Mature_109_residues
ATIEDFLKLDIRVGTVLKAEEFPEAKVPAIKLEIDFGGEIGVKCSSAQITKRYTPEKLVGRQVTAVVNFPPRRIAGFKSE
VLVLGGVPEKGDVILLAPDQEAPNGTKIS

Specific function: Could serves as a molecular chaperone for exported proteins or alternatively acts by stabilizing the secA protein [H]

COG id: COG0073

COG function: function code R; EMAP domain

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR008231
- InterPro:   IPR002547 [H]

Pfam domain/function: PF01588 tRNA_bind [H]

EC number: 6.1.1.10 [C]

Molecular weight: Translated: 11862; Mature: 11731

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIEDFLKLDIRVGTVLKAEEFPEAKVPAIKLEIDFGGEIGVKCSSAQITKRYTPEKLV
CCCHHHHHEEEEEECEEEECCCCCCCCCCEEEEEEECCCCCCEEECCCEEECCCCCHHHC
GRQVTAVVNFPPRRIAGFKSEVLVLGGVPEKGDVILLAPDQEAPNGTKIS
CCEEEEEEECCCHHHCCCCCCEEEEECCCCCCCEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
ATIEDFLKLDIRVGTVLKAEEFPEAKVPAIKLEIDFGGEIGVKCSSAQITKRYTPEKLV
CCHHHHHEEEEEECEEEECCCCCCCCCCEEEEEEECCCCCCEEECCCEEECCCCCHHHC
GRQVTAVVNFPPRRIAGFKSEVLVLGGVPEKGDVILLAPDQEAPNGTKIS
CCEEEEEEECCCHHHCCCCCCEEEEECCCCCCCEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mn2+; Zn2+ [C]

Kcat value (1/min): 198 [C]

Specific activity: 66.7

Km value (mM): 0.48 {Met}} 0.078 {Met}} 0.022 {Met}} 0.02 {Met}} 0.55 {ATP}} 0.41 {ATP}} 0.0009 {tRNA}} [C]

Substrates: ATP; @METH01.txt*L-methionine!; tRNAMet [C]

Specific reaction: ATP + @METH01.txt*L-methionine! + tRNAMet = AMP + diphosphate +L-methionyl-tRNAMet [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 1, 10-Phenanthroline; 2, 2'-Dipyridyl; Adenosine; NH4+; Periodate-oxidized ATP; Periodate-oxidized tRNA [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1435734; 9384377 [H]