BacMap

Definition	Bacillus licheniformis ATCC 14580, complete genome.
Accession	NC_006322
Length	4,222,645

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The map label for this gene is yngF [H]

Identifier: 52785891

GI number: 52785891

Start: 2089118

End: 2089900

Strand: Reverse

Name: yngF [H]

Synonym: BLi02140

Alternate gene names: 52785891

Gene position: 2089900-2089118 (Counterclockwise)

Preceding gene: 52785892

Following gene: 52785890

Centisome position: 49.49

GC content: 54.15

Gene sequence:

>783_bases
ATGGAGCCCAATGTTCTTTATTCCATAAACGAGCAGTCGGTCGCCGTGTTGACGCTGAACAGGCCGCAGGCTGCAAATGC
CCTCTCGCTTGGGCTTCTCGACGACTTTCAGCGCATCCTTCGAGATATTCGTTCAAACCCGGCCGTCCGCTGTGTCATCA
TAACGGGAAAAGGGGACAGGACGTTTTGTGCAGGCGCCGATTTAAAGGAAAGAGCCCGCATGAGCCAAACAGAAGCGAAG
CAGGCTGTTTCCCTGATTCAACGCGTGGTCAGCGAAACGGAAAAACTGCCGCAGCCCGTCATCGCTTCATTAAACGGAAG
CGCTTTAGGAGGGGGGCTGGAGCTTGCATTGGCGTGCGACATCAGGATCGCAGCCGAACATATTGAACTCGGCCTCCCCG
AAACAACGCTCGCAATCATTCCAGGGGCAGGAGGGACACAGCGGCTGCCCCGCTTGATCGGCAGGGGAAAGGCGAAAGAA
ATGATCTTTACCGGCTGCCGCATCAGCGCCGAAGAAGCGCAAAAGATCAGCCTGGTTGAACATGTCGTTCCGCTTTCGAA
GTTAAAGGAAGCGAGTGAAAGCATCGCGGCGAACATCGCGGCGAACGGACCGGTAGCCGTCAGACAAGCGAAGTTTGCCA
TCAATCAAGGCCTTGAGACAGCTATCGAAACAGGGCTTGCCATTGAACAAAAAGCCTATGAACTGACGATTCCGACGAAA
GACAGGACAGAAGGGCTGAAAGCTTTTGCAGAAAAGCGGAAGCCGGATTATACGGGAGAATAA

Upstream 100 bases:

>100_bases
AAATGGATCGAAGACAAAACCGGAAGAGAGCTGCCAAGCCGAAATTTGCAGGCGGCTAAATCAAAGAACTGTTTTCATTG
ATGAAGAGGAGGAAGACCGA

Downstream 100 bases:

>100_bases
AACGAACGGAGGGACAGCCGATGCCGGCCGAAAAGGAATTGTATGAACGCATCCGCCGGATTCAGGAGGGCGGCGCGGAG
AAATACCATGAAGCTAATAA

Product: enoyl-CoA hydratase

Products: trans-2-enoyl-CoA; trans-3-enoyl-CoA; H2O

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MEPNVLYSINEQSVAVLTLNRPQAANALSLGLLDDFQRILRDIRSNPAVRCVIITGKGDRTFCAGADLKERARMSQTEAK
QAVSLIQRVVSETEKLPQPVIASLNGSALGGGLELALACDIRIAAEHIELGLPETTLAIIPGAGGTQRLPRLIGRGKAKE
MIFTGCRISAEEAQKISLVEHVVPLSKLKEASESIAANIAANGPVAVRQAKFAINQGLETAIETGLAIEQKAYELTIPTK
DRTEGLKAFAEKRKPDYTGE

Sequences:

>Translated_260_residues
MEPNVLYSINEQSVAVLTLNRPQAANALSLGLLDDFQRILRDIRSNPAVRCVIITGKGDRTFCAGADLKERARMSQTEAK
QAVSLIQRVVSETEKLPQPVIASLNGSALGGGLELALACDIRIAAEHIELGLPETTLAIIPGAGGTQRLPRLIGRGKAKE
MIFTGCRISAEEAQKISLVEHVVPLSKLKEASESIAANIAANGPVAVRQAKFAINQGLETAIETGLAIEQKAYELTIPTK
DRTEGLKAFAEKRKPDYTGE
>Mature_260_residues
MEPNVLYSINEQSVAVLTLNRPQAANALSLGLLDDFQRILRDIRSNPAVRCVIITGKGDRTFCAGADLKERARMSQTEAK
QAVSLIQRVVSETEKLPQPVIASLNGSALGGGLELALACDIRIAAEHIELGLPETTLAIIPGAGGTQRLPRLIGRGKAKE
MIFTGCRISAEEAQKISLVEHVVPLSKLKEASESIAANIAANGPVAVRQAKFAINQGLETAIETGLAIEQKAYELTIPTK
DRTEGLKAFAEKRKPDYTGE

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI4502327, Length=253, Percent_Identity=49.802371541502, Blast_Score=206, Evalue=2e-53,
Organism=Homo sapiens, GI194097323, Length=259, Percent_Identity=40.1544401544402, Blast_Score=191, Evalue=5e-49,
Organism=Homo sapiens, GI150378541, Length=254, Percent_Identity=36.2204724409449, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI20127408, Length=232, Percent_Identity=36.6379310344828, Blast_Score=124, Evalue=6e-29,
Organism=Homo sapiens, GI68989263, Length=186, Percent_Identity=37.6344086021505, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI31542718, Length=249, Percent_Identity=32.9317269076305, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI70995211, Length=258, Percent_Identity=27.5193798449612, Blast_Score=95, Evalue=5e-20,
Organism=Homo sapiens, GI37594471, Length=191, Percent_Identity=29.8429319371728, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI37594469, Length=191, Percent_Identity=29.8429319371728, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI157694516, Length=226, Percent_Identity=28.3185840707965, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI213417737, Length=226, Percent_Identity=28.3185840707965, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI261878539, Length=84, Percent_Identity=51.1904761904762, Blast_Score=81, Evalue=8e-16,
Organism=Homo sapiens, GI157694520, Length=175, Percent_Identity=30.2857142857143, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI45643119, Length=211, Percent_Identity=25.1184834123223, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI260275230, Length=211, Percent_Identity=25.1184834123223, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI260274832, Length=206, Percent_Identity=25.2427184466019, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI116235487, Length=202, Percent_Identity=23.7623762376238, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787659, Length=252, Percent_Identity=41.2698412698413, Blast_Score=179, Evalue=1e-46,
Organism=Escherichia coli, GI221142681, Length=258, Percent_Identity=33.7209302325581, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1788682, Length=206, Percent_Identity=38.8349514563107, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1788597, Length=258, Percent_Identity=29.4573643410853, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1787660, Length=254, Percent_Identity=33.4645669291339, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI87082183, Length=256, Percent_Identity=29.6875, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1790281, Length=201, Percent_Identity=34.8258706467662, Blast_Score=107, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17540714, Length=223, Percent_Identity=46.6367713004484, Blast_Score=212, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI25145438, Length=247, Percent_Identity=38.4615384615385, Blast_Score=179, Evalue=9e-46,
Organism=Caenorhabditis elegans, GI17554946, Length=249, Percent_Identity=38.9558232931727, Blast_Score=175, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17558304, Length=177, Percent_Identity=36.1581920903955, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17536985, Length=260, Percent_Identity=26.1538461538462, Blast_Score=104, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17534483, Length=225, Percent_Identity=27.5555555555556, Blast_Score=97, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17508953, Length=234, Percent_Identity=31.6239316239316, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17508951, Length=234, Percent_Identity=31.6239316239316, Blast_Score=94, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI25144276, Length=234, Percent_Identity=31.6239316239316, Blast_Score=94, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI25144160, Length=185, Percent_Identity=33.5135135135135, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI25144157, Length=185, Percent_Identity=33.5135135135135, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17560910, Length=250, Percent_Identity=28.8, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17549921, Length=213, Percent_Identity=23.4741784037559, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI25141363, Length=200, Percent_Identity=28, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17540306, Length=257, Percent_Identity=28.7937743190661, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17535521, Length=190, Percent_Identity=27.3684210526316, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=191, Percent_Identity=25.130890052356, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24653139, Length=253, Percent_Identity=49.4071146245059, Blast_Score=241, Evalue=4e-64,
Organism=Drosophila melanogaster, GI20129971, Length=249, Percent_Identity=39.7590361445783, Blast_Score=186, Evalue=1e-47,
Organism=Drosophila melanogaster, GI24653477, Length=249, Percent_Identity=39.7590361445783, Blast_Score=186, Evalue=1e-47,
Organism=Drosophila melanogaster, GI19922422, Length=260, Percent_Identity=28.0769230769231, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI19920382, Length=222, Percent_Identity=29.7297297297297, Blast_Score=98, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24583077, Length=178, Percent_Identity=37.6404494382022, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24583079, Length=178, Percent_Identity=37.6404494382022, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI19921000, Length=178, Percent_Identity=37.6404494382022, Blast_Score=95, Evalue=6e-20,
Organism=Drosophila melanogaster, GI21357171, Length=261, Percent_Identity=28.3524904214559, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24654903, Length=176, Percent_Identity=27.8409090909091, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI28571729, Length=179, Percent_Identity=28.4916201117318, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI28571730, Length=179, Percent_Identity=28.4916201117318, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24650670, Length=174, Percent_Identity=30.4597701149425, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24583165, Length=174, Percent_Identity=27.5862068965517, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI19921018, Length=192, Percent_Identity=28.6458333333333, Blast_Score=68, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17

Molecular weight: Translated: 27861; Mature: 27861

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPNVLYSINEQSVAVLTLNRPQAANALSLGLLDDFQRILRDIRSNPAVRCVIITGKGDR
CCCCEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCC
TFCAGADLKERARMSQTEAKQAVSLIQRVVSETEKLPQPVIASLNGSALGGGLELALACD
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCEEEEEEEC
IRIAAEHIELGLPETTLAIIPGAGGTQRLPRLIGRGKAKEMIFTGCRISAEEAQKISLVE
EEEEHHHHHCCCCCCEEEEEECCCCHHHHHHHHCCCCCHHHEEECCCCCHHHHHHHHHHH
HVVPLSKLKEASESIAANIAANGPVAVRQAKFAINQGLETAIETGLAIEQKAYELTIPTK
HHHCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCEEEEECCCC
DRTEGLKAFAEKRKPDYTGE
CHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MEPNVLYSINEQSVAVLTLNRPQAANALSLGLLDDFQRILRDIRSNPAVRCVIITGKGDR
CCCCEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCC
TFCAGADLKERARMSQTEAKQAVSLIQRVVSETEKLPQPVIASLNGSALGGGLELALACD
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCEEEEEEEC
IRIAAEHIELGLPETTLAIIPGAGGTQRLPRLIGRGKAKEMIFTGCRISAEEAQKISLVE
EEEEHHHHHCCCCCCEEEEEECCCCHHHHHHHHCCCCCHHHEEECCCCCHHHHHHHHHHH
HVVPLSKLKEASESIAANIAANGPVAVRQAKFAINQGLETAIETGLAIEQKAYELTIPTK
HHHCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCEEEEECCCC
DRTEGLKAFAEKRKPDYTGE
CHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.05 {crotonyl-CoA}} 0.008 {2-decenoyl-CoA}} [C]

Substrates: (3S)-3-hydroxyacyl-CoA

Specific reaction: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O

General reaction: addition (of H2O to carbon carbon double bondelimination (of H2O C-O bond cleavagehydration [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]