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The map label for this gene is narH [H]

Identifier: 52785827

GI number: 52785827

Start: 2012157

End: 2013626

Strand: Reverse

Name: narH [H]

Synonym: BLi02073

Alternate gene names: 52785827

Gene position: 2013626-2012157 (Counterclockwise)

Preceding gene: 52785828

Following gene: 52785826

Centisome position: 47.69

GC content: 46.6

Gene sequence:

>1470_bases
TTGAAGATTAAAGCGCAAATCGGAATGGTAATGAACCTTGACAAATGCATCGGCTGCCATACATGCAGCGTCACATGCAA
AAACACTTGGACAAACCGTTCCGGTGCAGAATATATGTACTTCAACAACGTCGAAACAAAACCGGGCATCGGCTACCCTA
AACAATGGGAGAACCAGGATAAATACAAAGGCGGCTGGGAGCTGAAAAAAGGCAAGCTCCAGCTTAAATCAGGCGCCAAA
GCAACGCGGCTCATGAATTTATTCTACAACCCGTATCAGCCGACGATCGATGACTACTATGAACCTTGGAACTATGATTA
TGAAACGCTGACAAACAGTCCGGAGAAAAAACATCAGCCTGTTGCAAGGCCGAAGTCTTCCATTACGGGAGACTTCATGA
ACCTTGAATGGGGTCCGAACTGGGAAGACGATCTTGCCGGCGGCCACATTACAGGCCTCCAAGATCCAAACGTCAAAAAA
ATGGAAGAATCCATCAAAACCGAATTTGAAGACGTCTTTATGATGTATCTGCCAAGGATATGCGAACACTGCATCAATCC
TTCCTGCGTCTCCTCCTGTCCGTCGGGAGCGATGTACAAACGCGAGGAAGACGGCATCGTTCTCGTCGATCAAAATGCGT
GCCGCTCATGGAGACACTGTGTATCATCATGTCCTTATAAAAAAGTGTATTTCAACTGGCAGACTAACAAGGCGGAAAAA
TGCACATTGTGCTTCCCTCGTCTTGAAGCAGGACTGCCGACAATCTGCTCTGAGACATGCGTCGGCAGAATCCGCTATCT
CGGAGTCATGCTTTATGACGCAGAAAAAGTCGAAGAAGCGGCTTCAGCCGAAAATGAAAAAGATTTGTATCACTCACAGC
TGGAGATTTTCCTTGACCCGAATGATCCCGCTGTTGCAGAAGAAGCAAAAGCTCAAGGCATTCCGCTGGAGTGGATTGAA
GCAGCGCAAAAATCTCCGATCTACAAAATGATCATCGATTGGAAAATCGCTCTGCCGCTGCATCCTGAATACCGGACATT
GCCGATGGTATGGTATATTCCTCCGCTCAGTCCGATTATGAATATGTTTGAAGGAAAAGGAACAGCCCAATCAGCTGAAG
ATATCTTCCCTGCTATTGATGAAATGCGGATTCCGATCGAATATTTGGCCAATTTGCTGACTGCCGGTGATACACACCAT
ATTAGAACGACATTAAAGAAAATGTCTGTGATGCGTATGCACATGCGGGCGACCCAAACGAACAAACAGATCGATTCATC
TCTGATTCAAGATACCGGATTGACTGAAGAGCAGATTGAAGACATGTACCGTCTGCTTGCAATCGCCAAATACGATGACC
GGTTCGTCATTCCTCAAACCCATCGCGAAGAAGTCGCGGATCTATACATGGAACAAGGAAGCTGCGGATTATCGTTTGCC
GGAGGACCCGGCTCTTGCCAAAACATATAA

Upstream 100 bases:

>100_bases
GGCTACGGACAGCTCAGCTACGGATTCAACTACTACGGGCCGACAGGAAATCAGCGCGACCTCAATGTCGTGATCAGAAA
ATTGAAGGAGGTCGATTGGC

Downstream 100 bases:

>100_bases
ACACTGGAGGGAAGCTTCATGGATGGAACAGACAAACAAACCGTTTTTGCCGCTCTTTCTTACCTCCTCTCCTACCCTGA
TGAAGAGTGGAGAAAAGAAC

Product: NarH

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MKIKAQIGMVMNLDKCIGCHTCSVTCKNTWTNRSGAEYMYFNNVETKPGIGYPKQWENQDKYKGGWELKKGKLQLKSGAK
ATRLMNLFYNPYQPTIDDYYEPWNYDYETLTNSPEKKHQPVARPKSSITGDFMNLEWGPNWEDDLAGGHITGLQDPNVKK
MEESIKTEFEDVFMMYLPRICEHCINPSCVSSCPSGAMYKREEDGIVLVDQNACRSWRHCVSSCPYKKVYFNWQTNKAEK
CTLCFPRLEAGLPTICSETCVGRIRYLGVMLYDAEKVEEAASAENEKDLYHSQLEIFLDPNDPAVAEEAKAQGIPLEWIE
AAQKSPIYKMIIDWKIALPLHPEYRTLPMVWYIPPLSPIMNMFEGKGTAQSAEDIFPAIDEMRIPIEYLANLLTAGDTHH
IRTTLKKMSVMRMHMRATQTNKQIDSSLIQDTGLTEEQIEDMYRLLAIAKYDDRFVIPQTHREEVADLYMEQGSCGLSFA
GGPGSCQNI

Sequences:

>Translated_489_residues
MKIKAQIGMVMNLDKCIGCHTCSVTCKNTWTNRSGAEYMYFNNVETKPGIGYPKQWENQDKYKGGWELKKGKLQLKSGAK
ATRLMNLFYNPYQPTIDDYYEPWNYDYETLTNSPEKKHQPVARPKSSITGDFMNLEWGPNWEDDLAGGHITGLQDPNVKK
MEESIKTEFEDVFMMYLPRICEHCINPSCVSSCPSGAMYKREEDGIVLVDQNACRSWRHCVSSCPYKKVYFNWQTNKAEK
CTLCFPRLEAGLPTICSETCVGRIRYLGVMLYDAEKVEEAASAENEKDLYHSQLEIFLDPNDPAVAEEAKAQGIPLEWIE
AAQKSPIYKMIIDWKIALPLHPEYRTLPMVWYIPPLSPIMNMFEGKGTAQSAEDIFPAIDEMRIPIEYLANLLTAGDTHH
IRTTLKKMSVMRMHMRATQTNKQIDSSLIQDTGLTEEQIEDMYRLLAIAKYDDRFVIPQTHREEVADLYMEQGSCGLSFA
GGPGSCQNI
>Mature_489_residues
MKIKAQIGMVMNLDKCIGCHTCSVTCKNTWTNRSGAEYMYFNNVETKPGIGYPKQWENQDKYKGGWELKKGKLQLKSGAK
ATRLMNLFYNPYQPTIDDYYEPWNYDYETLTNSPEKKHQPVARPKSSITGDFMNLEWGPNWEDDLAGGHITGLQDPNVKK
MEESIKTEFEDVFMMYLPRICEHCINPSCVSSCPSGAMYKREEDGIVLVDQNACRSWRHCVSSCPYKKVYFNWQTNKAEK
CTLCFPRLEAGLPTICSETCVGRIRYLGVMLYDAEKVEEAASAENEKDLYHSQLEIFLDPNDPAVAEEAKAQGIPLEWIE
AAQKSPIYKMIIDWKIALPLHPEYRTLPMVWYIPPLSPIMNMFEGKGTAQSAEDIFPAIDEMRIPIEYLANLLTAGDTHH
IRTTLKKMSVMRMHMRATQTNKQIDSSLIQDTGLTEEQIEDMYRLLAIAKYDDRFVIPQTHREEVADLYMEQGSCGLSFA
GGPGSCQNI

Specific function: The Nitrate Reductase Enzyme Complex Allows E. coli To Use Nitrate As An Electron Acceptor During Anaerobic Growth. The Beta Chain Is An Electron Transfer Unit Containing Four Cysteine Clusters Involved In The Formation Of Iron-Sulfur Centers. Electrons A

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787478, Length=485, Percent_Identity=59.1752577319588, Blast_Score=640, Evalue=0.0,
Organism=Escherichia coli, GI1787740, Length=484, Percent_Identity=58.8842975206612, Blast_Score=634, Evalue=0.0,
Organism=Escherichia coli, GI1787872, Length=132, Percent_Identity=34.8484848484849, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI1787122, Length=132, Percent_Identity=34.8484848484849, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI2367345, Length=157, Percent_Identity=27.3885350318471, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI226510944, Length=86, Percent_Identity=38.3720930232558, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1789370, Length=103, Percent_Identity=33.9805825242718, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI1787749, Length=87, Percent_Identity=33.3333333333333, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 55882; Mature: 55882

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
8.0 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
8.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKAQIGMVMNLDKCIGCHTCSVTCKNTWTNRSGAEYMYFNNVETKPGIGYPKQWENQD
CEEHHHHCCEEEHHHHHCCCEEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
KYKGGWELKKGKLQLKSGAKATRLMNLFYNPYQPTIDDYYEPWNYDYETLTNSPEKKHQP
CCCCCCEEECCCEEECCCCHHHHHHHHHCCCCCCCHHHCCCCCCCCHHHHCCCCHHHCCC
VARPKSSITGDFMNLEWGPNWEDDLAGGHITGLQDPNVKKMEESIKTEFEDVFMMYLPRI
CCCCCCCCCCCEEECCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
CEHCINPSCVSSCPSGAMYKREEDGIVLVDQNACRSWRHCVSSCPYKKVYFNWQTNKAEK
HHHHCCCHHHHCCCCCCCEEECCCCEEEECCHHHHHHHHHHHHCCCEEEEEEEECCCCCE
CTLCFPRLEAGLPTICSETCVGRIRYLGVMLYDAEKVEEAASAENEKDLYHSQLEIFLDP
EEEECHHHHCCCCHHHHHHHHHHHHHHHHHEECHHHHHHHHCCCCHHHHHCCEEEEEECC
NDPAVAEEAKAQGIPLEWIEAAQKSPIYKMIIDWKIALPLHPEYRTLPMVWYIPPLSPIM
CCCCHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCEEEECCCHHHHH
NMFEGKGTAQSAEDIFPAIDEMRIPIEYLANLLTAGDTHHIRTTLKKMSVMRMHMRATQT
HHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
NKQIDSSLIQDTGLTEEQIEDMYRLLAIAKYDDRFVIPQTHREEVADLYMEQGSCGLSFA
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHCCCCCEEEC
GGPGSCQNI
CCCCCCCCC
>Mature Secondary Structure
MKIKAQIGMVMNLDKCIGCHTCSVTCKNTWTNRSGAEYMYFNNVETKPGIGYPKQWENQD
CEEHHHHCCEEEHHHHHCCCEEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
KYKGGWELKKGKLQLKSGAKATRLMNLFYNPYQPTIDDYYEPWNYDYETLTNSPEKKHQP
CCCCCCEEECCCEEECCCCHHHHHHHHHCCCCCCCHHHCCCCCCCCHHHHCCCCHHHCCC
VARPKSSITGDFMNLEWGPNWEDDLAGGHITGLQDPNVKKMEESIKTEFEDVFMMYLPRI
CCCCCCCCCCCEEECCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
CEHCINPSCVSSCPSGAMYKREEDGIVLVDQNACRSWRHCVSSCPYKKVYFNWQTNKAEK
HHHHCCCHHHHCCCCCCCEEECCCCEEEECCHHHHHHHHHHHHCCCEEEEEEEECCCCCE
CTLCFPRLEAGLPTICSETCVGRIRYLGVMLYDAEKVEEAASAENEKDLYHSQLEIFLDP
EEEECHHHHCCCCHHHHHHHHHHHHHHHHHEECHHHHHHHHCCCCHHHHHCCEEEEEECC
NDPAVAEEAKAQGIPLEWIEAAQKSPIYKMIIDWKIALPLHPEYRTLPMVWYIPPLSPIM
CCCCHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCEEEECCCHHHHH
NMFEGKGTAQSAEDIFPAIDEMRIPIEYLANLLTAGDTHHIRTTLKKMSVMRMHMRATQT
HHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
NKQIDSSLIQDTGLTEEQIEDMYRLLAIAKYDDRFVIPQTHREEVADLYMEQGSCGLSFA
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHCCCCCEEEC
GGPGSCQNI
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9353933; 7557333; 9384377 [H]