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The map label for this gene is narI [H]

Identifier: 52785825

GI number: 52785825

Start: 2010913

End: 2011599

Strand: Reverse

Name: narI [H]

Synonym: BLi02071

Alternate gene names: 52785825

Gene position: 2011599-2010913 (Counterclockwise)

Preceding gene: 52785826

Following gene: 52785824

Centisome position: 47.64

GC content: 49.05

Gene sequence:

>687_bases
ATGATCGAACAAATACTGTGGGTCATCCTCCCCTACATTGTCATCACCATTTTTATCGGAGGGCATATTTACCGCTATCA
ACATGATCAATTTGGCTGGACGGCCAAATCTAGCGAGATGCTCGAAAAGAAAAAACTTGCGCTTGGCAGCAGCCTTTTCC
ACTGGGGAATCTTTTTTGTCATCGGCGGACATGTGATGGGAATTTTAATTCCGGAAAGCCTTTATGAGGCGCTCGGCGTT
TCAGAGCATATGTACCATAAGATCGCGATCGGCTTCGGACTGCCTGCAGGCATCGCCGCGCTGTCAGGGCTTCTCATATT
GACCTACCGCCGATTCACGGACAAACGGATTCGAAAAACGAGCTCTGCCGGCGATTTAATCACCCTTGTCGCTTTGTTAT
TCGTGATGACCACTGGGCTTGCGGCAACCTTCTTAAACATCGATTCAAAAGGATTCGACTACAGGACAACAATCGGCCCG
TGGTTTCGCAACATTTTCTTATTCAAGCCGGATGCCGGGCTTATGGCGAGCGTGCCCCTCTGGTTTAAGCTTCACATCAT
CATGGGCTACGTCATTTTCATCGTCTGGCCGTTCACTCGGCTCGTCCACGTATTCAGCATGCCGCTAAAATATTTGACAA
GAAGCTATGTTGTTTACCGGAAACGGGCGCCCCGCAAAAGCATGTAA

Upstream 100 bases:

>100_bases
TGAGATCGCAGCTTTCAGAGAATGAAAGCATCTATACACCGTTATTGGACGGATTAATGCTGATCTTAGAGGAAATTGGT
GTTGAAAGGAATGTACAGCC

Downstream 100 bases:

>100_bases
ACATTTTCCCTAAAGCTAAAACGGCTTTAGGGATTTTTTTCTCCATAGGCAAGCTACCCATATTACCAATCAATCATACG
ATAAAGCAAACCATCAGAAA

Product: NarI

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MIEQILWVILPYIVITIFIGGHIYRYQHDQFGWTAKSSEMLEKKKLALGSSLFHWGIFFVIGGHVMGILIPESLYEALGV
SEHMYHKIAIGFGLPAGIAALSGLLILTYRRFTDKRIRKTSSAGDLITLVALLFVMTTGLAATFLNIDSKGFDYRTTIGP
WFRNIFLFKPDAGLMASVPLWFKLHIIMGYVIFIVWPFTRLVHVFSMPLKYLTRSYVVYRKRAPRKSM

Sequences:

>Translated_228_residues
MIEQILWVILPYIVITIFIGGHIYRYQHDQFGWTAKSSEMLEKKKLALGSSLFHWGIFFVIGGHVMGILIPESLYEALGV
SEHMYHKIAIGFGLPAGIAALSGLLILTYRRFTDKRIRKTSSAGDLITLVALLFVMTTGLAATFLNIDSKGFDYRTTIGP
WFRNIFLFKPDAGLMASVPLWFKLHIIMGYVIFIVWPFTRLVHVFSMPLKYLTRSYVVYRKRAPRKSM
>Mature_228_residues
MIEQILWVILPYIVITIFIGGHIYRYQHDQFGWTAKSSEMLEKKKLALGSSLFHWGIFFVIGGHVMGILIPESLYEALGV
SEHMYHKIAIGFGLPAGIAALSGLLILTYRRFTDKRIRKTSSAGDLITLVALLFVMTTGLAATFLNIDSKGFDYRTTIGP
WFRNIFLFKPDAGLMASVPLWFKLHIIMGYVIFIVWPFTRLVHVFSMPLKYLTRSYVVYRKRAPRKSM

Specific function: This Is A Second Nitrate Reductase Enzyme Which Can Substitute For The Nra Enzyme And Allows E.Coli To Use Nitrate As An Electron Acceptor During Anaerobic Growth. The Gamma Chain Is A Membrane-Embedded Heme-Iron Unit Resembling Cytochrome B, Which Trans

COG id: COG2181

COG function: function code C; Nitrate reductase gamma subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787738, Length=216, Percent_Identity=36.1111111111111, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI1787480, Length=216, Percent_Identity=34.2592592592593, Blast_Score=125, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003816 [H]

Pfam domain/function: PF02665 Nitrate_red_gam [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 26088; Mature: 26088

Theoretical pI: Translated: 10.47; Mature: 10.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEQILWVILPYIVITIFIGGHIYRYQHDQFGWTAKSSEMLEKKKLALGSSLFHWGIFFV
CHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
IGGHVMGILIPESLYEALGVSEHMYHKIAIGFGLPAGIAALSGLLILTYRRFTDKRIRKT
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
SSAGDLITLVALLFVMTTGLAATFLNIDSKGFDYRTTIGPWFRNIFLFKPDAGLMASVPL
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHEEEECCCCCCEECCHH
WFKLHIIMGYVIFIVWPFTRLVHVFSMPLKYLTRSYVVYRKRAPRKSM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIEQILWVILPYIVITIFIGGHIYRYQHDQFGWTAKSSEMLEKKKLALGSSLFHWGIFFV
CHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
IGGHVMGILIPESLYEALGVSEHMYHKIAIGFGLPAGIAALSGLLILTYRRFTDKRIRKT
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
SSAGDLITLVALLFVMTTGLAATFLNIDSKGFDYRTTIGPWFRNIFLFKPDAGLMASVPL
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHEEEECCCCCCEECCHH
WFKLHIIMGYVIFIVWPFTRLVHVFSMPLKYLTRSYVVYRKRAPRKSM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8846791; 9353933; 9384377; 7557333 [H]